0 Description of Workflow

Previous studies suggested that de novo assembly is beneficial for significantly differentially expressed genes (DEG) even when a reference genome is available. ^[1] Moreover, it is now widely recognized that performing transcript quantification and afterwards obtaining the gene expression by adding together the expression from the individual transcripts will result in improved gene-level analysis. ^[2,
3] Therefore, the current analysis for this bulk RNA-seq data set is based on the transcript-level with de novo transcriptome assembly. The current analysis protocol was modified from a guideline attached to a peer-reviewed bioinformatic software called IsoformSwitchAnalyzeR ^[4] (Please Click HERE to check the origianl protocol). Briefly, the current protocol can be described as a 6-step process:

After QC and potential quality trimming, the reads were mapped to the genome with the STAR aligner.

Then the de novo transcriptome assembly was performed with StringTie software and was guided by existing annoation from ENSEMBL database.

The third step is to merge the assembled transcripts from all individual StringTie runs into one combined reference transcriptome with StringTie software. The merged GTF file was further annotated with SQANTI3 software, which is designed to annotate the data generated from oxford nanopore sequencing. Meanwhile, the Circular RNA was detected with CIRCexplorer2 software ^[5] from the mapping results created in Step 1 based on the merged GTF file.

To make the analysis of the individual samples comparable, all samples were re-quantified using the merged GTF file created in Step 3 with a quasi-mapping aligner called Salmon.

Applying a “rescue” algorithm embbed in the IsoformSwitchAnalyzeR ^[4] software to the merged GTF file, to ensures that all novel isoforms are assigned to genes and that “reference gene_id” and “reference gene_names” are used instead of “Stringtie gene_is” for all already annotated genes.The “rescued” GTF file was annotated by SQANTI3 software again and the still remained novel genes (“Stringtie genes” not overlapping any “Refrence genes”) was removed for the downstream analysis.

Finally, after correction of batch effect by RUVSeq software, the significant differentially-expressed genes (DEGs) were detected by DESeq2 software; the significant differential exon usage (DEU) were detected by DEXseq software; the analysis of isoform switches with functional consequences and the associated alternative splicing was performed using IsoformSwitchAnalyzeR software; the significant differentially-expressed circular RNAs were detected by circRNAprofiler software.

The source code is avaible on the Supplementary section to reproduce this analysis.

In addition, other data set was generated in order to use the leafcutter software to check the alternative splicing at nucleotide level; to use the Ularcirc software to visulize, to predict ORF, and to estimate the potenial biological function of detected circular RNAs.

The above workflow is developed and mantained by Bioinformatics Study Group in Okayama University (BSGOU). BSGOU is an international academic community committed to advancing the digital transformation of biological and biomedical research. We unite students, researchers, clinicians, and engineers to collaboratively explore how high-throughput data and integrative computation can drive new theories, models, and discoveries in life sciences. For more information in details, please visit the homepage (https://labonom.github.io/) of BSGOU.

[1] Wang S, Gribskov M. Comprehensive evaluation of de novo transcriptome assembly programs and their effects on differential gene expression analysis. Bioinformatics. 2017 Feb 1;33(3):327-33.

[2] Soneson C, Love MI, Robinson MD. Differential analyses for RNA-seq: transcript-level estimates improve gene-level inferences. F1000Research. 2015;4.

[3] Yi L, Pimentel H, Bray NL, Pachter L. Gene-level differential analysis at transcript-level resolution. Genome biology. 2018 Dec;19(1):1-1.

[4] Vitting-Seerup K, Sandelin A. IsoformSwitchAnalyzeR: analysis of changes in genome-wide patterns of alternative splicing and its functional consequences. Bioinformatics. 2019 Nov 1;35(21):4469-71.

[5] Zhang XO, Dong R, Zhang Y, Zhang JL, Luo Z, Zhang J, Chen LL, Yang L. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs. Genome research. 2016 Sep 1;26(9):1277-87.

1 Sample Sequencing Statistics

1.1 Reference

Genome sequence reference:
- Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
Coding sequence reference:
- Homo_sapiens.GRCh38.cdna.all.fa.gz
Non-coding RNA reference:
- Homo_sapiens.GRCh38.ncrna.fa.gz
Annotation of features:
- Homo_sapiens.GRCh38.106.gtf.gz

1.2 Quality Control Report

Click HERE to check the FastQC results.

Click HERE to inspect the de novo transcriptome assembly results.

1.3 Quasi-Mapping by Salmon software

*Mapping Rate (%): the number of reads mapped on genome features (i.e. a genome region contains genes)

Automatically detected most likely library type as ISR.

Click HERE to learn more detials about the fragment library types.

Fragment Library Types

1.4 Correction of Batch Effect by RUVSeq

2 Analysis

2.1 Differential Gene Expression Analysis

Click HERE to check the read counts, TPM, and feature length of all mapped genes across all samples in a Microsoft .excel file. Click HERE to check all parameters of DESeq2 model for all detected genes of all samples in a Microsoft .excel file. Click HERE to check the DESeq2 model estimated mean gene expression level of each condition (the intercept of a linear model) and its standard errors. For more detials of the DESeq2 model, please Click HERE.

					Sig. AS
Overview of Differentially Expressed Genes (DEGs)
			Sig. DEGs				in sig. DEGs
	All Dectected Genes	Non Sig. DEGs	Up- DEGs	Down- DEGs	Genes with Sig. AS	in Non Sig. DEGs	in Up- DEGs	in Down- DEGs
HEK293_OSMI2_2hA vs HEK293_DMSO_2hA	16056	16054	2	0	467	466	1	0
HEK293_OSMI2_6hA vs HEK293_DMSO_6hA	16740	16678	2	60	922	915	0	7
HEK293_TMG_2hB vs HEK293_DMSO_2hB	15962	15962	0	0	536	536	0	0
HEK293_TMG_6hB vs HEK293_DMSO_6hB	15529	15506	1	22	490	490	0	0
HEK293_TMG_2hB vs HEK293_OSMI2_2hA	16486	8898	4359	3229	7120	3559	2439	1122
HEK293_TMG_6hB vs HEK293_OSMI2_6hA	16118	7387	4743	3988	3337	1343	1447	547
† TPM, Transcripts Per Kilobase Million; DEGs, Differentially-Expressed Genes; Sig., Significant; Up-DEGs, Significantly Up-Regulated Genes; Down-DEGs, Significantly Down-Regulated Genes; AS, Alternative Splicing

2.2 Functional Enrichment Analysis

	GSEA				ORA
Number of Significantlly-Enriched Terms of Functional Enrichment Analysis (FEA)
					GO-BP			GO-MF			GO-CC			KEGG
	BP	MF	CC	KEGG	From All Sig. DEGs	From Up- DEGs	From Down- DEGs	From All Sig. DEGs	From Up- DEGs	From Down- DEGs	From All Sig. DEGs	From Up- DEGs	From Down- DEGs	From All Sig. DEGs	From Up- DEGs	From Down- DEGs
HEK293_OSMI2_2hA vs HEK293_DMSO_2hA	71	32	24	3	23	1	6	5	23	1	6	5	0	0	0	0
HEK293_OSMI2_6hA vs HEK293_DMSO_6hA	226	48	32	44	208	41	39	0	0	0	0	0	208	41	39	0
HEK293_TMG_2hB vs HEK293_DMSO_2hB	158	66	43	15	0	0	0	0	0	0	0	0	0	0	0	0
HEK293_TMG_6hB vs HEK293_DMSO_6hB	88	42	38	14	10	4	4	10	0	0	0	0	10	4	4	10
HEK293_TMG_2hB vs HEK293_OSMI2_2hA	278	109	80	28	1281	268	266	102	940	228	175	42	693	136	149	89
HEK293_TMG_6hB vs HEK293_OSMI2_6hA	294	122	83	14	1427	275	267	124	1087	229	202	52	755	126	143	107
† GSEA, Gene Set Enrichment Analysis; ORA, Over Representation Analysis; DEGs, Differentially-Expressed Genes; GO-BP, Gene Ontology Biological Processes; GO-MF, Gene Ontology Molecular Function; GO-CC, Gene Ontology Cellular Component; KEGG, Kyoto Encyclopedia of Genes and Genomes; Sig., Significant; Up-DEGs, Significantly Up-Regulated Genes; Down-DEGs, Significantly Down-Regulated Genes

2.3 Analysis of Isoform Switches

Click HERE to download all results of the significantly-switched isoforms analysis (q < 0.05 and dif > 0.05).

Click HERE to download the alternative splicing analysis results for visualization by LeafCutter software.

Amount of Event

Enrichment Analysis

Comparison Analysis

Abbreviations

tss : Test transcripts for whether they use different Transcription Start Site (TSS) (more than ntCutoff from each other).
tts : Test transcripts for whether they use different Transcription Termination Site (TTS) (more than ntCutoff from each other).
last_exon : Test whether transcripts utilizes different last exons (defined as the last exon of each transcript is non-overlapping).
isoform_seq_similarity : Test whether the isoform nucleotide sequences are different (as described above). Reported as different if the measured JCsim is smaller than ntJCsimCutoff and the length difference of the aligned and combined region is larger than ntCutoff.
isoform_length : Test transcripts for differences in isoform length. Only reported if the difference is larger than indicated by the ntCutoff and ntFracCutoff. Please * note that this is a less powerful analysis than implemented in ‘isoform_seq_similarity’ as two equally long sequences might be very different.
exon_number : Test transcripts for differences in exon number.
intron_structure : Test transcripts for differences in intron structure, e.g. usage of exon-exon junctions. This analysis corresponds to analyzing whether all introns in one isoform is also found in the other isoforms.
intron_retention : Test for differences in intron retentions (and their genomic positions). Require that analyzeIntronRetention have been run.
isoform_class_code : Test transcripts for differences in the transcript classification provide by cufflinks. For a updated list of class codes see http://cole-trapnell-lab.github.io/cufflinks/cuffcompare/#transfrag-class-codes.
coding_potential : Test transcripts for differences in coding potential, as indicated by the CPAT or CPC2 analysis. Requires that analyzeCPAT or analyzeCPC2 have been used to add external conding potential analysis to the switchAnalyzeRlist.
ORF_seq_similarity : Test whether the amino acid sequences of the ORFs are different (as described above). Reported as different if the measured JCsim is smaller than AaJCsimCutoff and the length difference of the aligned and combined region is larger than AaCutoff. Requires that least one of the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
ORF_genomic : Test transcripts for differences in genomic position of the Open Reading Frames (ORF). Requires that least one of the isoforms are annotated with an ORF either via identifyORF or by supplying a GTF file and settingaddAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
ORF_length : Test transcripts for differences in length of Open Reading Frames (ORF). Note that this is a less powerful analysis than implemented in ORF_seq_similarity as two equally long sequences might be very different. Only reported if the difference is larger than indicated by the ntCutoff and ntFracCutoff. Requires that least one of the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
5_utr_seq_similarity : Test whether the isoform nucleotide sequences of the 5’ UnTranslated Region (UTR) are different (as described above). The 5’UTR is defined as the region from the transcript start to the ORF start. Reported as different if the measured JCsim is smaller than ntJCsimCutoff and the length difference of the aligned and combined region is larger than ntCutoff. Requires that both the isoforms are annotated with an ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
5_utr_length : Test transcripts for differences in the length of the 5’ UnTranslated Region (UTR). The 5’UTR is defined as the region from the transcript start to the ORF start. Note that this is a less powerful analysis than implemented in ‘5_utr_seq_similarity’ as two equally long sequences might be very different. Only reported if the difference is larger than indicated by the ntCutoff and ntFracCutoff. Requires that both the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
3_utr_seq_similarity : Test whether the isoform nucleotide sequences of the 3’ UnTranslated Region (UTR) are different (as described above). The 3’UTR is defined as the region from the end of the ORF to the transcript end. Reported as different if the measured JCsim is smaller than ntJCsimCutoff and the length difference of the aligned and combined region is larger than ntCutoff. Requires that both the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
3_utr_length : Test transcripts for differences in the length of the 3’ UnTranslated Regions (UTR). The 3’UTR is defined as the region from the end of the ORF to the transcript end. Note that this is a less powerful analysis than implemented in 3_utr_seq_similarity as two equally long sequences might be very different. Requires that identifyORF have been used to predict NMD sensitivity or that the ORF was imported though one of the dedicated import functions implemented in isoformSwitchAnalyzeR. Only reported if the difference is larger than indicated by the ntCutoff and ntFracCutoff. Requires that both the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
NMD_status : Test transcripts for differences in sensitivity to Nonsense Mediated Decay (NMD). Requires that both the isoforms have been annotated with PTC either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
domains_identified : Test transcripts for differences in the name and order of which domains are identified by the Pfam in the transcripts. Requires that analyzePFAM have been used to add external Pfam analysis to the switchAnalyzeRlist. Requires that both the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
domain_length : Test transcripts for differences in the length of overlapping domains of the same type (same hmm_name) thereby enabling analysis of protein domain truncation. Do however note that a small difference in length is will likely not truncate the protein domain. The length difference, measured in AA, must be larger than AaCutoff and AaFracCutoff .Requires that analyzePFAM have been used to add external Pfam analysis to the switchAnalyzeRlist. Requires that both the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist.
genomic_domain_position : Test transcripts for differences in the genomic position of the domains identified by the Pfam analysis (Will be different unless the two isoforms have the same domains at the same genomic location). Requires that analyzePFAM have been used to add external Pfam analysis to the switchAnalyzeRlist. Requires that both the isoforms are annotated with a ORF either via identifyORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist (and are thereby also affected by removeNoncodinORFs=TRUE in analyzeCPAT).
IDR_identified : Test for differences in isoform IDRs. Specifically the two isoforms are analyzed for whether they contain IDRs which do not overlap in genomic coordinates. Requires that analyzeNetSurfP2 or analyzeIUPred2A have been used to add external IDR analysis to the switchAnalyzeRlist.
IDR_length : Test for differences in the length of overlapping (in genomic coordinates) IDRs. The length difference, measured in AA, must be larger than AaCutoff and AaFracCutoff. Requires that analyzeNetSurfP2 or analyzeIUPred2A have been used to add external IDR analysis to the switchAnalyzeRlist.
IDR_type : Test for differences in IDR type. Specifically the two isoforms are tested for overlapping IDRs (genomic coordinates) and overlapping IDRs are compared with regards to their IDR type (IDR vs IDR w binding site). Only available if analyzeIUPred2A was used to add external IDR analysis to the switchAnalyzeRlist.
signal_peptide_identified : Test transcripts for differences in whether a signal peptide was identified or not by the SignalP analysis. Requires that analyzeSignalP have been used to add external SignalP analysis to the switchAnalyzeRlist. Requires that both the isoforms are annotated with a ORF either via analyzeORF or by supplying a GTF file and setting addAnnotatedORFs=TRUE when creating the switchAnalyzeRlist (and are thereby also affected by removeNoncodinORFs=TRUE in analyzeCPAT).

Volcano Plot

2.4 Circular RNA Analysis

Click HERE to download the circRNA analysis results for visualization by Ularcirc software. For using the Ularcirc software, please also download and install the corresponding BSgenome, TxDb, and annotation packages in your R enviroment.

id	gene	allTranscripts	transcript	totExons	strand	chrom	startUpIntron	endUpIntron	startUpBSE	endUpBSE	startDownBSE	endDownBSE	startDownIntron	endDownIntron	exNumUpBSE	exNumDownBSE	numOfExons	lenUpIntron	lenUpBSE	lenDownBSE	lenDownIntron	meanLengthBSEs	meanLengthIntrons	lenCircRNA	Read # on BSJ
ENSG00000065526:+:1:15909320:15936266	ENSG00000065526	MSTRG.376.9,ENST00000375759,MSTRG.376.10,MSTRG.376.4,MSTRG.376.6,MSTRG.376.3,MSTRG.376.5	MSTRG.376.9	14	+	1	15876679	15909320	15909321	15909481	15928091	15936266	15936267	15937162	3	10	8	32641	160	8175	895	4167.5	16768	9145	25
ENSG00000219481:-:1:16564806:16567351	ENSG00000219481	ENST00000430580,MSTRG.409.2,MSTRG.409.3,MSTRG.409.8,MSTRG.409.9	ENST00000430580	29	-	1	16567978	16567352	16567351	16567179	16564918	16564807	16564806	16564187	25	28	4	626	172	111	619	141.5	622.5	510	25
ENSG00000271254:-:KI270711.1:6990:9537	ENSG00000271254	MSTRG.33771.1,ENST00000614336,MSTRG.33771.4,MSTRG.33771.13,MSTRG.33771.6,MSTRG.33771.5,MSTRG.33771.8,MSTRG.33771.7,MSTRG.33771.11,MSTRG.33771.9,MSTRG.33771.14,ENST00000612640,MSTRG.33771.12	MSTRG.33771.1	22	-	KI270711.1	10139	9538	9537	9365	7102	6991	6990	6371	18	21	4	601	172	111	619	141.5	610	510	22
ENSG00000271254:-:KI270711.1:6990:11083	ENSG00000271254	MSTRG.33771.1,MSTRG.33771.3,ENST00000614336,MSTRG.33771.13,MSTRG.33771.6,MSTRG.33771.7,MSTRG.33771.9,MSTRG.33771.14,MSTRG.33771.10,ENST00000612640,MSTRG.33771.12	MSTRG.33771.1	22	-	KI270711.1	11706	11084	11083	10911	7102	6991	6990	6371	16	21	6	622	172	111	619	141.5	620.5	735	13
ENSG00000075151:-:1:20849414:20855071	ENSG00000075151	ENST00000684485,MSTRG.488.12,ENST00000685872,ENST00000602326,MSTRG.488.11,ENST00000686579,MSTRG.488.10,MSTRG.488.18,MSTRG.488.14,ENST00000400422,ENST00000688741,ENST00000693470,MSTRG.488.20,MSTRG.488.13,MSTRG.488.26,ENST00000681717,MSTRG.488.22,MSTRG.488.25,MSTRG.488.24,MSTRG.488.21,ENST00000374935,MSTRG.488.23,ENST00000681767,MSTRG.488.17,MSTRG.488.19,ENST00000264211,ENST00000685814,ENST00000634879,MSTRG.488.27	ENST00000684485	36	-	1	20857402	20855072	20855071	20854978	20849530	20849415	20849414	20841029	25	28	4	2330	93	115	8385	104	5357.5	549	13
ENSG00000147202:+:X:96945526:96965207	ENSG00000147202	ENST00000324765,MSTRG.34576.4,ENST00000373049,MSTRG.34576.3	ENST00000324765	27	+	X	96942137	96945526	96945527	96945591	96965093	96965207	96965208	97072940	14	17	4	3389	64	114	107732	89	55560.5	606	9
ENSG00000197275:-:8:94458267:94467555	ENSG00000197275	ENST00000336148,MSTRG.31822.3,ENST00000297592,ENST00000463267,ENST00000523839	ENST00000336148	15	-	8	94475000	94467556	94467555	94467405	94458436	94458268	94458267	94411316	2	3	2	7444	150	168	46951	159	27197.5	320	5
ENSG00000198363:-:8:61680967:61684188	ENSG00000198363	ENST00000517856,ENST00000379454,ENST00000356457,ENST00000445642,ENST00000519234,ENST00000518068,MSTRG.31562.2,MSTRG.31562.10,MSTRG.31562.4,MSTRG.31562.11,MSTRG.31562.9,MSTRG.31562.16,MSTRG.31562.12,MSTRG.31562.8,ENST00000517903,MSTRG.31562.3,MSTRG.31562.6,MSTRG.31562.15,MSTRG.31562.5,MSTRG.31562.7,ENST00000541428,MSTRG.31562.13,ENST00000517847,MSTRG.31562.30,ENST00000379449,ENST00000389204,ENST00000522835,MSTRG.31562.34,MSTRG.31562.33,ENST00000522603,ENST00000517661,ENST00000518306,ENST00000522349,ENST00000517928	ENST00000517856	4	-	8	61714268	61684189	61684188	61684039	61681036	61680968	61680967	61661927	2	3	2	30079	149	68	19040	108.5	24559.5	219	5
ENSG00000213468:-:X:131749305:131794466	ENSG00000213468	ENST00000649541,ENST00000663405,MSTRG.34881.9,ENST00000427391,ENST00000665076,ENST00000637577,ENST00000654978,MSTRG.34881.6,MSTRG.34881.5,MSTRG.34881.19,MSTRG.34881.8,ENST00000647966,MSTRG.34881.2,MSTRG.34881.12,MSTRG.34881.7,MSTRG.34881.13,MSTRG.34881.26,MSTRG.34881.20,MSTRG.34881.24,MSTRG.34881.10,MSTRG.34881.27,MSTRG.34881.11	ENST00000649541	12	-	X	131825221	131794467	131794466	131794324	131749458	131749306	131749305	131692637	3	11	9	30754	142	152	56668	147	43711	1191	5
ENSG00000158417:+:2:99360235:99371730	ENSG00000158417	MSTRG.18937.2,MSTRG.18937.1,ENST00000617677,MSTRG.18937.4,ENST00000289371,MSTRG.18937.6,MSTRG.18937.5,MSTRG.18937.9	MSTRG.18937.2	24	+	2	99337590	99360235	99360236	99360361	99371662	99371730	99371731	99376346	2	9	8	22645	125	68	4615	96.5	13630	1515	5
ENSG00000146555:+:7:4161785:4178586	ENSG00000146555	ENST00000404826,MSTRG.29412.5,ENST00000389531,MSTRG.29412.4,MSTRG.29412.3,ENST00000476701	ENST00000404826	45	+	7	4158552	4161785	4161786	4161856	4178485	4178586	4178587	4205878	32	35	4	3233	70	101	27291	85.5	15262	369	4
ENSG00000180198_ENSG00000180098:+:1:28508127:28553737	ENSG00000180198_ENSG00000180098	MSTRG.692.8	MSTRG.692.8	22	+	1	28506085	28508127	28508128	28508160	28553640	28553737	28553738	28560632	2	15	14	2042	32	97	6894	64.5	4468	2693	4
ENSG00000136938:+:9:98011270:98012472	ENSG00000136938	MSTRG.33115.2,MSTRG.33115.4,MSTRG.33115.6,MSTRG.33115.5,MSTRG.33115.9,ENST00000339399,MSTRG.33115.3,MSTRG.33115.7,MSTRG.33115.8,MSTRG.33115.10,MSTRG.33115.12,MSTRG.33115.15,MSTRG.33115.18	MSTRG.33115.2	7	+	9	98005154	98011273	98011274	98011389	98012421	98012472	98012473	98015365	5	6	2	6119	115	51	2892	83	4505.5	168	4
ENSG00000163468:-:1:156310557:156310641	ENSG00000163468	ENST00000368261	ENST00000368261	6	-	1	156317535	156310642	156310641	156310558	156310641	156310558	156310557	156309304	5	5	1	6893	83	83	1253	83	4073	84	3
ENSG00000181163:+:5:171391304:171400210	ENSG00000181163	ENST00000676625,ENST00000678267,ENST00000676613,ENST00000678186,ENST00000677467,ENST00000677672,ENST00000677600,ENST00000678280,ENST00000521260,ENST00000677904,ENST00000676589,ENST00000518587,ENST00000677357,ENST00000517671,ENST00000677325,ENST00000678774,ENST00000296930,ENST00000521672,ENST00000351986,ENST00000677907,MSTRG.27289.27,ENST00000393820,ENST00000679006	ENST00000676625	10	+	5	171390131	171391304	171391305	171391424	171400153	171400210	171400211	171400838	3	7	5	1173	119	57	627	88	900	444	3
ENSG00000124788:-:6:16326393:16328470	ENSG00000124788	ENST00000244769,ENST00000436367	ENST00000244769	9	-	6	16485971	16328471	16328470	16326394	16328470	16326394	16326393	16306860	8	8	1	157500	2076	2076	19533	2076	88516.5	2077	3
ENSG00000198034:-:X:72275052:72276234	ENSG00000198034	MSTRG.34456.5,ENST00000316084,MSTRG.34456.2,MSTRG.34456.3,MSTRG.34456.4,ENST00000373626	MSTRG.34456.5	8	-	X	72276790	72276235	72276234	72276157	72275150	72275053	72275052	72273973	2	4	3	555	77	97	1079	87	817	357	3
ENSG00000184903:-:7:111487237:111487341	ENSG00000184903	ENST00000405709,ENST00000447215,ENST00000331762,MSTRG.30505.7,ENST00000452895,ENST00000492938,ENST00000437687	ENST00000405709	6	-	7	111521312	111487342	111487341	111487238	111487341	111487238	111487237	110963566	3	3	1	33970	103	103	523671	103	278820.5	104	3
ENSG00000109919:-:11:47622700:47639051	ENSG00000109919	ENST00000302503,MSTRG.5479.1,MSTRG.5479.7,MSTRG.5479.8,MSTRG.5479.6,MSTRG.5479.12,MSTRG.5479.11,MSTRG.5479.9,MSTRG.5479.2,MSTRG.5479.10,MSTRG.5479.13,MSTRG.5479.16,MSTRG.5479.14,MSTRG.5479.3	ENST00000302503	13	-	11	47642378	47639052	47639051	47638967	47622776	47622701	47622700	47618920	2	12	11	3326	84	75	3780	79.5	3553	738	2
ENSG00000159692:-:4:1241324:1241519	ENSG00000159692	ENST00000382952,MSTRG.24434.3,MSTRG.24434.6,MSTRG.24434.5,MSTRG.24434.9,MSTRG.24434.15,MSTRG.24434.19,MSTRG.24434.14,MSTRG.24434.8,MSTRG.24434.16,MSTRG.24434.13,MSTRG.24434.17,MSTRG.24434.26,ENST00000514210,ENST00000515399,ENST00000515690,ENST00000513420	ENST00000382952	10	-	4	1248915	1241520	1241519	1241325	1241519	1241325	1241324	1238338	2	2	1	7395	194	194	2986	194	5190.5	195	2
ENSG00000118922:-:13:73943980:73995053	ENSG00000118922	ENST00000377669,MSTRG.8871.2,ENST00000472022	ENST00000377669	8	-	13	74133738	73995054	73995053	73994990	73944070	73943981	73943980	73846374	2	3	2	138684	63	89	97606	76	118145	154	2
ENSG00000143748:-:1:224303722:224305166	ENSG00000143748	MSTRG.3125.12,MSTRG.3125.2,ENST00000482491,MSTRG.3125.1,ENST00000281701,ENST00000391875,ENST00000469075,ENST00000469968,MSTRG.3125.15,ENST00000340871,ENST00000467882	MSTRG.3125.12	25	-	1	224307990	224305167	224305166	224305034	224303857	224303723	224303722	224301772	8	10	3	2823	132	134	1950	133	2386.5	345	2
ENSG00000086758:-:X:53645310:53654131	ENSG00000086758	MSTRG.34313.1,ENST00000262854,ENST00000342160,MSTRG.34313.26,MSTRG.34313.6,MSTRG.34313.27,MSTRG.34313.9,MSTRG.34313.8,ENST00000612484,MSTRG.34313.10,MSTRG.34313.24,MSTRG.34313.12,MSTRG.34313.14,MSTRG.34313.13,MSTRG.34313.15,MSTRG.34313.20,MSTRG.34313.19,MSTRG.34313.7,MSTRG.34313.25,MSTRG.34313.23,MSTRG.34313.16,MSTRG.34313.21,MSTRG.34313.17,ENST00000218328	MSTRG.34313.1	84	-	X	53680048	53654132	53654131	53654063	53645463	53645311	53645310	53634299	3	6	4	25916	68	152	11011	110	18463.5	528	2
ENSG00000163635:+:3:63912587:63913225	ENSG00000163635	ENST00000295900,ENST00000674280,ENST00000487717,MSTRG.23192.3,MSTRG.23192.17,ENST00000474112,MSTRG.23192.7,MSTRG.23192.6,MSTRG.23192.16,MSTRG.23192.19,ENST00000522345,MSTRG.23192.11	ENST00000295900	13	+	3	63898498	63912587	63912588	63912923	63913157	63913225	63913226	63952378	3	4	2	14089	335	68	39152	201.5	26620.5	405	2
ENSG00000127022:+:5:179719667:179719781	ENSG00000127022	ENST00000679523,ENST00000680614,ENST00000681168,ENST00000679395,ENST00000680013,ENST00000681076,ENST00000681712,ENST00000679527,ENST00000681903,ENST00000502296,ENST00000513246,ENST00000680042,ENST00000680092,ENST00000681072,ENST00000680984,ENST00000681733,ENST00000681762,ENST00000680406,ENST00000680894,ENST00000681504,ENST00000681894,ENST00000681674,ENST00000680504,ENST00000452673,ENST00000247461,ENST00000681576,ENST00000680618,ENST00000680812,ENST00000680985,ENST00000679471,ENST00000681476,ENST00000680837,ENST00000681431,ENST00000679642,ENST00000681398,ENST00000680006,ENST00000681342,ENST00000506654,ENST00000680827,ENST00000681265,MSTRG.27443.29,MSTRG.27443.24,ENST00000509563,ENST00000504734,ENST00000514032,ENST00000502673	ENST00000679523	14	+	5	179716295	179719667	179719668	179719781	179719668	179719781	179719782	179720403	9	9	1	3372	113	113	621	113	1996.5	114	2
ENSG00000104267:+:8:85465271:85465469	ENSG00000104267	MSTRG.31765.1,ENST00000285379,ENST00000520127,ENST00000522742,ENST00000520996,ENST00000518231	MSTRG.31765.1	8	+	8	85464116	85465271	85465272	85465469	85465272	85465469	85465470	85473692	3	3	1	1155	197	197	8222	197	4688.5	198	2
ENSG00000151292:+:5:123545416:123557564	ENSG00000151292	MSTRG.26826.1,MSTRG.26826.3,MSTRG.26826.2,ENST00000345990,MSTRG.26826.4,MSTRG.26826.7,MSTRG.26826.10,MSTRG.26826.11,ENST00000510842,ENST00000521364	MSTRG.26826.1	12	+	5	123512571	123545416	123545417	123545841	123557495	123557564	123557565	123573392	2	4	3	32845	424	69	15827	246.5	24336	536	2
ENSG00000117114:+:1:81836884:81837057	ENSG00000117114	ENST00000674492,ENST00000674373,ENST00000370725,ENST00000370723,ENST00000370728,ENST00000370727,ENST00000370730,ENST00000370721,MSTRG.1489.45,MSTRG.1489.47,ENST00000674307,ENST00000686636,MSTRG.1489.46,ENST00000674407,MSTRG.1489.49,ENST00000370717,ENST00000674209,ENST00000370715,ENST00000627151,MSTRG.1489.38,MSTRG.1489.35,MSTRG.1489.31,ENST00000674442,ENST00000674168,ENST00000674419,ENST00000674208,ENST00000674393,MSTRG.1489.48,MSTRG.1489.43,MSTRG.1489.26,MSTRG.1489.37,ENST00000319517,MSTRG.1489.32,ENST00000359929,MSTRG.1489.52,MSTRG.1489.34,ENST00000370713,MSTRG.1489.51,MSTRG.1489.50,MSTRG.1489.41,MSTRG.1489.33,ENST00000674489,ENST00000469377,ENST00000674367,ENST00000674410,ENST00000674333,MSTRG.1489.44	ENST00000674492	24	+	1	81800480	81836884	81836885	81837057	81836885	81837057	81837058	81907016	2	2	1	36404	172	172	69958	172	53181	173	2
ENSG00000102572:-:13:98519242:98519473	ENSG00000102572	ENST00000539966,ENST00000397517,ENST00000376547,ENST00000444574,ENST00000481288,ENST00000491878	ENST00000539966	11	-	13	98576744	98519474	98519473	98519243	98519473	98519243	98519242	98482322	2	2	1	57270	230	230	36920	230	47095	231	2
ENSG00000198492:+:1:28742402:28743986	ENSG00000198492	ENST00000478283,ENST00000542507,ENST00000373812,ENST00000541996,ENST00000474884,ENST00000496288,ENST00000468863,ENST00000476976,ENST00000475796	ENST00000478283	4	+	1	28738339	28742402	28742403	28743986	28742403	28743986	28743987	28768928	3	3	1	4063	1583	1583	24941	1583	14502	1584	2
ENSG00000139746:-:13:79319534:79378907	ENSG00000139746	MSTRG.8946.3,MSTRG.8946.4,MSTRG.8946.7,MSTRG.8946.6,MSTRG.8946.8,MSTRG.8946.11,MSTRG.8946.10,MSTRG.8946.12,MSTRG.8946.14,MSTRG.8946.17,MSTRG.8946.19,MSTRG.8946.32,MSTRG.8946.22,MSTRG.8946.30,MSTRG.8946.21,MSTRG.8946.27,MSTRG.8946.29,MSTRG.8946.26,MSTRG.8946.31	MSTRG.8946.3	23	-	13	79405703	79378908	79378907	79378789	79320710	79319535	79319534	79318880	2	21	20	26795	118	1175	654	646.5	13724.5	3967	2
ENSG00000188092:+:1:147986198:147988521	ENSG00000188092	ENST00000488603,ENST00000478307,ENST00000488165,ENST00000314163,ENST00000490955,MSTRG.2061.1	ENST00000488603	7	+	1	147969960	147986198	147986199	147986294	147988432	147988521	147988522	147992501	4	5	2	16238	95	89	3979	92	10108.5	186	2
ENSG00000157933:+:1:2302977:2304585	ENSG00000157933	MSTRG.129.1,ENST00000378536	MSTRG.129.1	7	+	1	2229736	2302977	2302978	2303103	2304293	2304585	2304586	2306019	2	5	4	73241	125	292	1433	208.5	37337	804	2
ENSG00000170035:+:2:180982017:180984093	ENSG00000170035	MSTRG.19601.17,MSTRG.19601.13,ENST00000410062,MSTRG.19601.16,ENST00000602710,MSTRG.19601.15,ENST00000392415,MSTRG.19601.4,MSTRG.19601.19,MSTRG.19601.3,ENST00000602959,MSTRG.19601.2,MSTRG.19601.10,MSTRG.19601.6,ENST00000602291,ENST00000602479,ENST00000602499,ENST00000602762,ENST00000602303,ENST00000602475	MSTRG.19601.17	8	+	2	180981478	180982017	180982018	180982236	180984043	180984093	180984094	181057692	2	3	2	539	218	50	73598	134	37068.5	270	2
ENSG00000114416:+:3:180968050:180970358	ENSG00000114416	ENST00000305586,ENST00000475315,ENST00000480918,ENST00000357559,ENST00000472339,ENST00000445140,ENST00000468861,ENST00000491062,ENST00000482125	ENST00000305586	18	+	3	180963091	180968050	180968051	180968254	180970158	180970358	180970359	180975312	15	16	2	4959	203	200	4953	201.5	4956	405	2
ENSG00000102024:+:X:115610242:115610323	ENSG00000102024	ENST00000481823,MSTRG.34749.1,MSTRG.34749.4,ENST00000289290,ENST00000355899,ENST00000539310,ENST00000489283,ENST00000626746,ENST00000473026	ENST00000481823	17	+	X	115593633	115610242	115610243	115610323	115610243	115610323	115610324	115614152	2	2	1	16609	80	80	3828	80	10218.5	81	2
ENSG00000112699:-:6:1726415:1742586	ENSG00000112699	ENST00000380805,ENST00000380815,ENST00000530927,ENST00000531690	ENST00000380805	6	-	6	1930102	1742587	1742586	1742468	1726512	1726416	1726415	1717644	2	3	2	187515	118	96	8771	107	98143	216	2
ENSG00000156110:+:10:74394140:74398579	ENSG00000156110	ENST00000673310,ENST00000372734,ENST00000539909,ENST00000673352,ENST00000672394,ENST00000672920,MSTRG.4212.10,ENST00000541550,ENST00000286621,ENST00000672429,ENST00000673027,MSTRG.4212.9,ENST00000672604	ENST00000673310	12	+	10	74314746	74394140	74394141	74394313	74398471	74398579	74398580	74525255	5	6	2	79394	172	108	126675	140	103034.5	282	2
ENSG00000197157:+:7:127807483:127991056	ENSG00000197157	ENST00000354725,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.2,MSTRG.30622.4	ENST00000354725	24	+	7	127721401	127807483	127807484	127807573	127990947	127991056	127991057	128074501	11	16	6	86082	89	109	83444	99	84763	627	2
ENSG00000147403:+:X:154399337:154399941	ENSG00000147403	MSTRG.35107.3,MSTRG.35107.4,MSTRG.35107.1,ENST00000485196,ENST00000344746,ENST00000467168,ENST00000436473,ENST00000406022,ENST00000369817,ENST00000458500	MSTRG.35107.3	7	+	X	154398543	154399337	154399338	154399396	154399803	154399941	154399942	154400701	4	6	3	794	58	138	759	98	776.5	306	2
ENSG00000141556:+:17:82763964:82814934	ENSG00000141556	ENST00000681983,ENST00000684361,ENST00000684760,ENST00000684349,ENST00000683444,ENST00000682479,ENST00000684464,ENST00000684429,ENST00000539345,ENST00000355528,ENST00000682722,ENST00000684000,ENST00000683041,ENST00000684544,ENST00000683282,ENST00000684188,ENST00000682213,ENST00000684408,ENST00000683009,ENST00000682921	ENST00000681983	38	+	17	82756216	82763964	82763965	82764062	82814840	82814934	82814935	82870223	3	13	11	7748	97	94	55288	95.5	31518	1083	2
ENSG00000074855:-:19:17333100:17333800	ENSG00000074855	MSTRG.16681.9,MSTRG.16681.25,MSTRG.16681.11,MSTRG.16681.17,MSTRG.16681.18,MSTRG.16681.29,MSTRG.16681.23,ENST00000597643,MSTRG.16681.16,ENST00000159087,MSTRG.16681.5,MSTRG.16681.20,MSTRG.16681.30,ENST00000630631	MSTRG.16681.9	15	-	19	17334564	17333801	17333800	17333690	17333239	17333101	17333100	17333027	2	4	3	763	110	138	73	124	418	383	2
ENSG00000229807:-:X:73826114:73833374	ENSG00000229807	ENST00000650627,ENST00000650186,ENST00000647696,ENST00000669898,ENST00000650637,MSTRG.34462.12,MSTRG.34462.1,ENST00000635841,ENST00000434839	ENST00000650627	8	-	X	73837439	73833375	73833374	73833238	73827984	73826115	73826114	73822217	3	6	4	4064	136	1869	3897	1002.5	3980.5	2380	2
ENSG00000128573:+:7:114162943:114288109	ENSG00000128573	ENST00000441290,MSTRG.30535.2,MSTRG.30535.3,MSTRG.30535.5,ENST00000635638,MSTRG.30535.10,MSTRG.30535.7,ENST00000440349,ENST00000495516	ENST00000441290	23	+	7	114086612	114162943	114162944	114163088	114288019	114288109	114288110	114366504	2	4	3	76331	144	90	78394	117	77362.5	288	2
ENSG00000128710:+:2:176108906:176109358	ENSG00000128710	MSTRG.19550.2,MSTRG.19550.1,MSTRG.19550.13,MSTRG.19550.19,MSTRG.19550.14,MSTRG.19550.21,MSTRG.19550.20,MSTRG.19550.23,MSTRG.19550.24	MSTRG.19550.2	4	+	2	176104627	176108906	176108907	176109358	176108907	176109358	176109359	176111382	2	2	1	4279	451	451	2023	451	3151	452	2
ENSG00000169855:-:3:78938600:78938927	ENSG00000169855	ENST00000436010,ENST00000618846,MSTRG.23281.4,ENST00000464233,MSTRG.23281.5,ENST00000495273,ENST00000467549	ENST00000436010	29	-	3	79018406	78938928	78938927	78938601	78938927	78938601	78938600	78746901	2	2	1	79478	326	326	191699	326	135588.5	327	2
ENSG00000258539:-:10:124681606:124706887	ENSG00000258539	ENST00000494792	ENST00000494792	10	-	10	124760385	124706888	124706887	124706636	124682379	124681607	124681606	124618794	7	9	3	53497	251	772	62812	511.5	58154.5	1080	2
ENSG00000144224:+:2:135769768:135772547	ENSG00000144224	MSTRG.19269.2,ENST00000272638,ENST00000490163,MSTRG.19269.1	MSTRG.19269.2	14	+	2	135761912	135769768	135769769	135769823	135772420	135772547	135772548	135776248	7	9	3	7856	54	127	3700	90.5	5778	348	2
ENSG00000108953:-:17:1400046:1400461	ENSG00000108953	MSTRG.13353.1,MSTRG.13353.8,MSTRG.13353.9,MSTRG.13353.6,MSTRG.13353.10,MSTRG.13353.14	MSTRG.13353.1	7	-	17	1403448	1400462	1400461	1400047	1400461	1400047	1400046	1365059	2	2	1	2986	414	414	34987	414	18986.5	415	2
ENSG00000249319:+:7:66127703:66134374	ENSG00000249319	ENST00000450043	ENST00000450043	12	+	7	66092664	66127703	66127704	66127740	66134280	66134374	66134375	66145442	8	10	3	35039	36	94	11067	65	23053	231	2
ENSG00000042753:-:19:46839464:46839578	ENSG00000042753	MSTRG.17376.5,ENST00000599990,ENST00000601498,ENST00000263270,ENST00000593442,ENST00000597020	MSTRG.17376.5	7	-	19	46845992	46839579	46839578	46839465	46839578	46839465	46839464	46838800	5	5	1	6413	113	113	664	113	3538.5	114	2
ENSG00000106443:+:7:10982371:10990847	ENSG00000106443	ENST00000403050,ENST00000490957,ENST00000634607,MSTRG.29511.9	ENST00000403050	17	+	7	10974946	10982371	10982372	10983159	10990703	10990847	10990848	11013746	3	4	2	7425	787	144	22898	465.5	15161.5	933	2
ENSG00000150403:+:13:113539356:113539507	ENSG00000150403	ENST00000434316,MSTRG.9106.3,ENST00000465556	ENST00000434316	13	+	13	113534241	113539356	113539357	113539507	113539357	113539507	113539508	113547299	10	10	1	5115	150	150	7791	150	6453	151	2
ENSG00000122566:-:7:26197314:26197732	ENSG00000122566	ENST00000476233,ENST00000678075,ENST00000678035,ENST00000356674,ENST00000677839,ENST00000676932,ENST00000354667,ENST00000618183,ENST00000679021,ENST00000677906,ENST00000677669,ENST00000679123,ENST00000677574,ENST00000678277,ENST00000679124,ENST00000678183,ENST00000677571,ENST00000678973,ENST00000677321,ENST00000678962,ENST00000678501,ENST00000679001,ENST00000678449,ENST00000677339,ENST00000608362,ENST00000679243,ENST00000678431,ENST00000677656,ENST00000676903,ENST00000677631,ENST00000678675,ENST00000676749,ENST00000676524,ENST00000676497,MSTRG.29609.62,ENST00000676746,ENST00000678935,ENST00000678998,ENST00000677396,ENST00000678697,ENST00000678631,MSTRG.29609.8,ENST00000678779,MSTRG.29609.65,MSTRG.29609.30,ENST00000360787,MSTRG.29609.70,ENST00000679318,MSTRG.29609.51,ENST00000677037,ENST00000678884,MSTRG.29609.67,MSTRG.29609.50,MSTRG.29609.66,MSTRG.29609.72,MSTRG.29609.69,MSTRG.29609.52,MSTRG.29609.53,MSTRG.29609.49,MSTRG.29609.74,MSTRG.29609.71,MSTRG.29609.55,MSTRG.29609.11,MSTRG.29609.57,MSTRG.29609.60,MSTRG.29609.59,MSTRG.29609.63,MSTRG.29609.68,MSTRG.29609.9,MSTRG.29609.10,MSTRG.29609.73,ENST00000495810	ENST00000476233	9	-	7	26200571	26197733	26197732	26197622	26197461	26197315	26197314	26197018	2	3	2	2838	110	146	296	128	1567	258	2
ENSG00000100726:+:16:1500095:1500699	ENSG00000100726	MSTRG.11782.12,MSTRG.11782.11,MSTRG.11782.14,ENST00000262319,MSTRG.11782.4,MSTRG.11782.6,MSTRG.11782.2,MSTRG.11782.8,MSTRG.11782.13,MSTRG.11782.10,MSTRG.11782.5,MSTRG.11782.3,MSTRG.11782.9	MSTRG.11782.12	21	+	16	1499334	1500095	1500096	1500164	1500563	1500699	1500700	1501419	7	9	3	761	68	136	719	102	740	348	2
ENSG00000115947:-:2:147972738:147975975	ENSG00000115947	ENST00000535373,ENST00000264169,ENST00000392857,MSTRG.19312.6,MSTRG.19312.7,ENST00000457954,ENST00000495601,ENST00000416719,ENST00000440042,MSTRG.19312.19	ENST00000535373	15	-	2	147981927	147975976	147975975	147975902	147972829	147972739	147972738	147958867	3	5	3	5951	73	90	13871	81.5	9911	242	2
ENSG00000122566:-:7:26193250:26193598	ENSG00000122566	ENST00000678973	ENST00000678973	12	-	7	26195846	26193599	26193598	26193575	26193373	26193251	26193250	26192578	8	9	2	2247	23	122	672	72.5	1459.5	147	2
ENSG00000102181:-:X:150795417:150814936	ENSG00000102181	ENST00000346693,ENST00000370377,MSTRG.35038.5,ENST00000418547	ENST00000346693	12	-	X	150816006	150814937	150814936	150814862	150795486	150795418	150795417	150795290	5	6	2	1069	74	68	127	71	598	144	2
ENSG00000267748:+:19:38283626:38287935	ENSG00000267748	MSTRG.17086.1	MSTRG.17086.1	7	+	19	38244135	38283626	38283627	38283797	38287876	38287935	38287936	38289137	2	3	2	39491	170	59	1201	114.5	20346	231	2
ENSG00000198162:+:1:117405548:117466427	ENSG00000198162	ENST00000356554,MSTRG.1897.3,MSTRG.1897.2	ENST00000356554	13	+	1	117402442	117405548	117405549	117405645	117466334	117466427	117466428	117493146	3	8	6	3106	96	93	26718	94.5	14912	610	2
ENSG00000249859:+:8:127939507:128010444	ENSG00000249859	ENST00000660438,ENST00000523068,ENST00000660631,ENST00000657449,ENST00000660456,ENST00000664265,ENST00000659912,ENST00000662413,ENST00000667305,ENST00000662410,ENST00000657289	ENST00000660438	13	+	8	127932475	127939507	127939508	127939676	128010318	128010444	128010445	128016634	6	12	7	7032	168	126	6189	147	6610.5	964	2
ENSG00000061938:-:3:195878252:195888606	ENSG00000061938	ENST00000333602,MSTRG.24313.1,ENST00000672887,ENST00000673420,ENST00000673038,ENST00000672024,ENST00000428187,ENST00000671753,ENST00000381916,MSTRG.24313.9,MSTRG.24313.15,MSTRG.24313.13,MSTRG.24313.14,ENST00000673374,ENST00000439230	ENST00000333602	15	-	3	195908484	195888607	195888606	195888426	195878347	195878253	195878252	195872471	2	9	8	19877	180	94	5781	137	12829	1274	2
ENSG00000164087:-:3:52122378:52125181	ENSG00000164087	ENST00000296484,ENST00000474012,ENST00000394970,MSTRG.23031.1,MSTRG.23031.7,MSTRG.23031.2,MSTRG.23031.6	ENST00000296484	11	-	3	52138168	52125182	52125181	52125113	52122477	52122379	52122378	52096713	8	9	2	12986	68	98	25665	83	19325.5	168	2
ENSG00000137145:+:9:19352072:19361963	ENSG00000137145	ENST00000602925,ENST00000434457,ENST00000380437,ENST00000494124,ENST00000380427,ENST00000361024	ENST00000602925	32	+	9	19350880	19352072	19352073	19352182	19361846	19361963	19361964	19369836	24	29	6	1192	109	117	7872	113	4532	1029	2
ENSG00000153406:-:16:4466152:4469465	ENSG00000153406	ENST00000574733,MSTRG.11971.4,MSTRG.11971.8,MSTRG.11971.1,ENST00000404295,ENST00000574425,ENST00000283429	ENST00000574733	6	-	16	4474092	4469466	4469465	4469227	4466402	4466153	4466152	4463851	3	4	2	4626	238	249	2301	243.5	3463.5	489	2
ENSG00000031823:-:19:5941620:5957973	ENSG00000031823	MSTRG.16270.6,ENST00000340578,ENST00000439268,MSTRG.16270.12,MSTRG.16270.1,MSTRG.16270.2,ENST00000592771,ENST00000591092,ENST00000034275,ENST00000587263,ENST00000591124,ENST00000588010,ENST00000592133,ENST00000585339,ENST00000587159,ENST00000589353,ENST00000587463	MSTRG.16270.6	16	-	19	5978938	5957974	5957973	5957918	5941707	5941621	5941620	5933480	2	4	3	20964	55	86	8140	70.5	14552	180	2
ENSG00000116918:+:1:231554620:231561255	ENSG00000116918	MSTRG.3284.12	MSTRG.3284.12	7	+	1	231542612	231554620	231554621	231554810	231561128	231561255	231561256	231564527	5	6	2	12008	189	127	3271	158	7639.5	318	1
ENSG00000117984:-:11:1753802:1753880	ENSG00000117984	ENST00000637387	ENST00000637387	9	-	11	1753993	1753881	1753880	1753803	1753880	1753803	1753802	1753671	8	8	1	112	77	77	131	77	121.5	78	1
ENSG00000048828:+:9:93471140:93498886	ENSG00000048828	ENST00000277165,MSTRG.33016.2,MSTRG.33016.4,MSTRG.33016.6,MSTRG.33016.5,MSTRG.33016.8,MSTRG.33016.9,MSTRG.33016.10,ENST00000375389,ENST00000446420	ENST00000277165	18	+	9	93452390	93471140	93471141	93471387	93498790	93498886	93498887	93515666	2	5	4	18750	246	96	16779	171	17764.5	556	1
ENSG00000172809:+:17:74209186:74209309	ENSG00000172809	MSTRG.15029.1,ENST00000533498,ENST00000439590,ENST00000311111,ENST00000584577	MSTRG.15029.1	5	+	17	74204191	74209186	74209187	74209309	74209187	74209309	74209310	74209803	4	4	1	4995	122	122	493	122	2744	123	1
ENSG00000115464:-:2:61278164:61288877	ENSG00000115464	ENST00000398571,MSTRG.18490.4,MSTRG.18490.6	ENST00000398571	80	-	2	61293463	61288878	61288877	61288677	61278285	61278165	61278164	61266168	34	41	8	4585	200	120	11996	160	8290.5	885	1
ENSG00000115053:-:2:231456630:231457124	ENSG00000115053	ENST00000322723,ENST00000461347,ENST00000453992,ENST00000678849,ENST00000678828,ENST00000676913,ENST00000679348,MSTRG.20060.9,ENST00000678246,MSTRG.20060.2,ENST00000678405,ENST00000417652,MSTRG.20060.21,ENST00000678131,MSTRG.20060.8,ENST00000676690,ENST00000356936,ENST00000678364,MSTRG.20060.20,ENST00000454824,ENST00000677703,ENST00000436894	ENST00000322723	14	-	2	231457642	231457125	231457124	231457001	231456764	231456631	231456630	231456137	10	11	2	517	123	133	493	128	505	258	1
ENSG00000159140:+:21:33546212:33555391	ENSG00000159140	MSTRG.21290.1,ENST00000455528,ENST00000356577,ENST00000381679,ENST00000300278	MSTRG.21290.1	12	+	21	33543170	33546212	33546213	33546379	33549476	33555391	33555392	33557155	2	3	2	3042	166	5915	1763	3040.5	2402.5	6083	1
ENSG00000186260:+:16:14140543:14140760	ENSG00000186260	ENST00000571589,ENST00000318282,MSTRG.12103.9,MSTRG.12103.10,MSTRG.12103.4,MSTRG.12103.2,ENST00000575833,MSTRG.12103.8,ENST00000574045,ENST00000575537,ENST00000574998	ENST00000571589	17	+	16	14079355	14140543	14140544	14140760	14140544	14140760	14140761	14210242	3	3	1	61188	216	216	69481	216	65334.5	217	1
ENSG00000115484:-:2:61872081:61883548	ENSG00000115484	ENST00000394440,MSTRG.18473.3,MSTRG.18473.6,MSTRG.18473.8,MSTRG.18473.5	ENST00000394440	14	-	2	61885019	61883549	61883548	61883459	61872316	61872082	61872081	61869554	3	12	10	1470	89	234	2527	161.5	1998.5	1311	1
ENSG00000117592:+:1:173485360:173486401	ENSG00000117592	MSTRG.2603.3,ENST00000340385,ENST00000470017,MSTRG.2603.1	MSTRG.2603.3	5	+	1	173481483	173485360	173485361	173485504	173486255	173486401	173486402	173487734	3	4	2	3877	143	146	1332	144.5	2604.5	291	1
ENSG00000206559:+:3:28478813:28492173	ENSG00000206559	MSTRG.22640.1,ENST00000383768,ENST00000419130	MSTRG.22640.1	11	+	3	28435270	28478813	28478814	28478931	28492127	28492173	28492174	28514063	6	7	2	43543	117	46	21889	81.5	32716	165	1
ENSG00000106443:+:7:10982371:11022979	ENSG00000106443	ENST00000403050,ENST00000490957,ENST00000634607,MSTRG.29511.9	ENST00000403050	17	+	7	10974946	10982371	10982372	10983159	11022868	11022979	11022980	11028680	3	6	4	7425	787	111	5700	449	6562.5	1205	1
ENSG00000153786:-:16:84990303:84996007	ENSG00000153786	ENST00000313732,ENST00000344861,MSTRG.13156.3,ENST00000564466	ENST00000313732	8	-	16	85011285	84996008	84996007	84995922	84990635	84990304	84990303	84981995	2	3	2	15277	85	331	8308	208	11792.5	418	1
ENSG00000106683:+:7:74096621:74099238	ENSG00000106683	ENST00000418310,ENST00000336180,ENST00000538333,ENST00000435201,ENST00000491052	ENST00000418310	16	+	7	74085845	74096621	74096622	74096760	74099032	74099238	74099239	74105874	3	5	3	10776	138	206	6635	172	8705.5	456	1
ENSG00000122687:-:7:2239417:2239676	ENSG00000122687	MSTRG.29392.35	MSTRG.29392.35	3	-	7	2242161	2239677	2239676	2239418	2239676	2239418	2239417	2235565	2	2	1	2484	258	258	3852	258	3168	259	1
ENSG00000083720:-:5:41749526:41862750	ENSG00000083720	ENST00000196371	ENST00000196371	17	-	5	41870280	41862751	41862750	41862642	41749607	41749527	41749526	41739492	2	15	14	7529	108	80	10034	94	8781.5	1341	1
ENSG00000136448:+:17:45081643:45086652	ENSG00000136448	ENST00000587014,ENST00000676753,ENST00000592782,ENST00000678576,ENST00000678332,ENST00000258960,ENST00000678938,ENST00000591931,ENST00000677949,ENST00000678606,ENST00000592654,ENST00000676828,MSTRG.14480.4,ENST00000590114,ENST00000590310,ENST00000543908,ENST00000585561	ENST00000587014	10	+	17	45061461	45081643	45081644	45081752	45086508	45086652	45086653	45093684	2	3	2	20182	108	144	7031	126	13606.5	254	1
ENSG00000070961:-:12:89655678:89656107	ENSG00000070961	MSTRG.7745.3,ENST00000428670,ENST00000261173,MSTRG.7745.6,MSTRG.7745.7,ENST00000549585	MSTRG.7745.3	21	-	12	89709218	89656108	89656107	89655679	89656107	89655679	89655678	89642356	2	2	1	53110	428	428	13322	428	33216	429	1
ENSG00000079246:+:2:216141185:216162048	ENSG00000079246	ENST00000460284,ENST00000392133,ENST00000392132,MSTRG.19917.6	ENST00000460284	18	+	2	216138180	216141185	216141186	216141319	216161979	216162048	216162049	216190224	10	13	4	3005	133	69	28175	101	15590	492	1
ENSG00000171843:-:9:20448122:20456786	ENSG00000171843	ENST00000380338,ENST00000630269	ENST00000380338	11	-	9	20620653	20456787	20456786	20456704	20448266	20448123	20448122	20414426	3	4	2	163866	82	143	33696	112.5	98781	227	1
ENSG00000117868:-:7:158788003:158799072	ENSG00000117868	ENST00000275418,ENST00000251527,ENST00000652148,ENST00000483958	ENST00000275418	23	-	7	158829088	158799073	158799072	158799031	158788093	158788004	158788003	158773397	2	6	5	30015	41	89	14606	65	22310.5	417	1
ENSG00000184465_ENSG00000272848:-:6:169602218:169652008	ENSG00000184465_ENSG00000272848	MSTRG.29337.7	MSTRG.29337.7	27	-	6	169658275	169652009	169652008	169651930	169602321	169602219	169602218	169582935	16	23	8	6266	78	102	19283	90	12774.5	790	1
ENSG00000104738:+:8:47967364:47970057	ENSG00000104738	ENST00000262105,ENST00000649973,MSTRG.31497.2,ENST00000648519,ENST00000649838,ENST00000649919,MSTRG.31497.6,ENST00000648407,ENST00000520637	ENST00000262105	16	+	8	47966408	47967364	47967365	47967485	47969798	47970057	47970058	47970510	9	10	2	956	120	259	452	189.5	704	381	1
ENSG00000135250:-:7:105132790:105203785	ENSG00000135250	ENST00000357311,ENST00000393651,MSTRG.30452.3,MSTRG.30452.2,MSTRG.30452.9,ENST00000489828	ENST00000357311	15	-	7	105268805	105203786	105203785	105203628	105132898	105132791	105132790	105127063	2	12	11	65019	157	107	5727	132	35373	1681	1
ENSG00000156170:+:8:94957994:94958179	ENSG00000156170	ENST00000396113,MSTRG.31849.10,MSTRG.31849.21,MSTRG.31849.22,MSTRG.31849.4,MSTRG.31849.1,MSTRG.31849.5,MSTRG.31849.19,MSTRG.31849.15,MSTRG.31849.18,MSTRG.31849.3,MSTRG.31849.12,MSTRG.31849.17,MSTRG.31849.14,MSTRG.31849.13,MSTRG.31849.8,MSTRG.31849.2,MSTRG.31849.16,MSTRG.31849.9,ENST00000523378,ENST00000519136,MSTRG.31849.20,MSTRG.31849.7	ENST00000396113	15	+	8	94945620	94957994	94957995	94958179	94957995	94958179	94958180	94975317	3	3	1	12374	184	184	17137	184	14755.5	185	1
ENSG00000136938:+:9:98011273:98012472	ENSG00000136938	MSTRG.33115.2,MSTRG.33115.4,MSTRG.33115.6,MSTRG.33115.5,MSTRG.33115.9,ENST00000339399,MSTRG.33115.3,MSTRG.33115.7,MSTRG.33115.8,MSTRG.33115.10,MSTRG.33115.12,MSTRG.33115.15,MSTRG.33115.18	MSTRG.33115.2	7	+	9	98005154	98011273	98011274	98011389	98012421	98012472	98012473	98015365	5	6	2	6119	115	51	2892	83	4505.5	168	1
ENSG00000127663:+:19:5039835:5047669	ENSG00000127663	ENST00000611640,ENST00000159111,MSTRG.16238.4,ENST00000536461,ENST00000381759	ENST00000611640	24	+	19	5033032	5039835	5039836	5040011	5047476	5047669	5047670	5071009	4	6	3	6803	175	193	23339	184	15071	485	1
ENSG00000283341:+:11:43750910:43754121	ENSG00000283341	ENST00000532864,ENST00000529261	ENST00000532864	11	+	11	43641035	43750910	43750911	43750957	43754046	43754121	43754122	43798319	4	5	2	109875	46	75	44197	60.5	77036	123	1
ENSG00000167658:-:19:3979328:3982424	ENSG00000167658	ENST00000309311,MSTRG.16189.5,MSTRG.16189.4,MSTRG.16189.7,MSTRG.16189.1,MSTRG.16189.6	ENST00000309311	15	-	19	3982806	3982425	3982424	3982246	3979436	3979329	3979328	3978173	5	11	7	381	178	107	1155	142.5	768	1101	1
ENSG00000007923:-:1:6667708:6681037	ENSG00000007923	MSTRG.209.1,ENST00000689474,ENST00000690452,ENST00000472414,ENST00000686243,ENST00000685129,ENST00000688524,ENST00000690062,ENST00000377577,ENST00000685485,ENST00000294401,ENST00000688162,ENST00000691444,MSTRG.209.17,ENST00000451196,ENST00000426784,ENST00000473993,ENST00000485073,ENST00000460594	MSTRG.209.1	17	-	1	6701728	6681038	6681037	6680908	6667810	6667709	6667708	6654040	2	4	3	20690	129	101	13668	115	17179	306	1
ENSG00000133059:-:1:205187417:205187806	ENSG00000133059	ENST00000367162,ENST00000367161,MSTRG.2916.3	ENST00000367162	13	-	1	205211270	205187807	205187806	205187418	205187806	205187418	205187417	205169833	2	2	1	23463	388	388	17584	388	20523.5	389	1
ENSG00000164045:-:3:48174283:48177988	ENSG00000164045	ENST00000302506,ENST00000351231,MSTRG.22890.5,MSTRG.22890.1,MSTRG.22890.7,MSTRG.22890.6,MSTRG.22890.4	ENST00000302506	15	-	3	48180720	48177989	48177988	48177854	48174457	48174284	48174283	48167945	7	9	3	2731	134	173	6338	153.5	4534.5	381	1
ENSG00000172915:+:13:35040932:35045401	ENSG00000172915	ENST00000379939,ENST00000400445,MSTRG.8591.8,ENST00000691351,ENST00000685717	ENST00000379939	59	+	13	34943115	35040932	35040933	35041164	35045306	35045401	35045402	35048562	2	4	3	97817	231	95	3160	163	50488.5	429	1
ENSG00000174720:+:4:112638617:112644871	ENSG00000174720	MSTRG.25335.14,ENST00000651579	MSTRG.25335.14	14	+	4	112637240	112638617	112638618	112638673	112644668	112644871	112644872	112646350	2	3	2	1377	55	203	1478	129	1427.5	260	1
ENSG00000002822:-:7:2014501:2069338	ENSG00000002822	ENST00000399654,ENST00000406869,ENST00000265854,MSTRG.29392.10,MSTRG.29392.15,ENST00000402746,MSTRG.29392.11,MSTRG.29392.12,MSTRG.29392.14,MSTRG.29392.13,ENST00000438959,ENST00000421113,ENST00000444373	ENST00000399654	19	-	7	2149151	2069339	2069338	2069194	2014642	2014502	2014501	2002122	12	13	2	79812	144	140	12379	142	46095.5	286	1
ENSG00000131467:+:17:42837648:42839380	ENSG00000131467	ENST00000545225,ENST00000441946,ENST00000541124,ENST00000590720,ENST00000293362,ENST00000543428,ENST00000592169,ENST00000589469	ENST00000545225	13	+	17	42834877	42837648	42837649	42837697	42839294	42839380	42839381	42841497	7	12	6	2771	48	86	2116	67	2443.5	441	1
ENSG00000123066:-:12:116237467:116237705	ENSG00000123066	ENST00000281928,MSTRG.8086.3,ENST00000650226,ENST00000548743,ENST00000551197,ENST00000647567,ENST00000648593,ENST00000648067,ENST00000650496,ENST00000649627	ENST00000281928	31	-	12	116277059	116237706	116237705	116237468	116237705	116237468	116237467	116111513	2	2	1	39353	237	237	125954	237	82653.5	238	1
ENSG00000102606:+:13:111217678:111233293	ENSG00000102606	ENST00000317133,ENST00000375741,ENST00000646102,ENST00000375739,ENST00000218789,MSTRG.9077.2,ENST00000375736,ENST00000375723,ENST00000426073,MSTRG.9077.14,MSTRG.9077.13,MSTRG.9077.12,MSTRG.9077.9,ENST00000466143,ENST00000469877	ENST00000317133	19	+	13	111210003	111217678	111217679	111217880	111233205	111233293	111233294	111243871	5	6	2	7675	201	88	10577	144.5	9126	291	1
ENSG00000137834:+:15:66711667:66716498	ENSG00000137834	MSTRG.11086.3,MSTRG.11086.5,MSTRG.11086.2,ENST00000288840,ENST00000557916,MSTRG.11086.10,MSTRG.11086.11,MSTRG.11086.6,MSTRG.11086.9,MSTRG.11086.1	MSTRG.11086.3	5	+	15	66708739	66711667	66711668	66711724	66716421	66716498	66716499	66780996	3	4	2	2928	56	77	64497	66.5	33712.5	135	1
ENSG00000015133:-:14:91305764:91309986	ENSG00000015133	ENST00000389857	ENST00000389857	30	-	14	91313079	91309987	91309986	91309859	91305926	91305765	91305764	91303979	16	19	4	3092	127	161	1785	144	2438.5	621	1
ENSG00000156860:+:16:30664214:30666541	ENSG00000156860	ENST00000356166,MSTRG.12479.1,MSTRG.12479.3,MSTRG.12479.7	ENST00000356166	18	+	16	30662860	30664214	30664215	30664516	30666512	30666541	30666542	30666918	7	12	6	1354	301	29	376	165	865	748	1
ENSG00000130311:+:19:17314022:17315995	ENSG00000130311	ENST00000359866,ENST00000593466,MSTRG.16680.1,ENST00000596582	ENST00000359866	5	+	19	17309658	17314022	17314023	17314103	17315934	17315995	17315996	17319545	2	4	3	4364	80	61	3549	70.5	3956.5	195	1
ENSG00000130414:-:2:239990073:240022340	ENSG00000130414	ENST00000678188,ENST00000678562,ENST00000476216,ENST00000677395,ENST00000678468,ENST00000677764,ENST00000677979,MSTRG.20164.20,ENST00000252711,ENST00000678455,ENST00000678914,ENST00000676784,ENST00000677294,ENST00000677155,ENST00000485344,ENST00000678898,ENST00000678289,ENST00000678158,ENST00000676929,ENST00000676491,ENST00000677407,ENST00000448880,ENST00000677490,ENST00000677263,ENST00000404554,ENST00000444548,ENST00000679183,ENST00000620965,ENST00000679308,ENST00000677567,ENST00000677114,MSTRG.20164.35,MSTRG.20164.1,ENST00000676782,ENST00000307300,MSTRG.20164.40,MSTRG.20164.6,ENST00000443626	ENST00000678188	10	-	2	240025226	240022341	240022340	240022172	239990182	239990074	239990073	239961187	2	9	8	2885	168	108	28886	138	15885.5	4057	1
ENSG00000124541:+:6:43024984:43029190	ENSG00000124541	MSTRG.28286.2,MSTRG.28286.1	MSTRG.28286.2	8	+	6	43021785	43024984	43024985	43025132	43029092	43029190	43029191	43033852	2	7	6	3199	147	98	4661	122.5	3930	660	1
ENSG00000141298:-:17:29648143:29672129	ENSG00000141298	ENST00000540801,ENST00000269033,ENST00000649863,MSTRG.14041.5	ENST00000540801	16	-	17	29676819	29672130	29672129	29671935	29648344	29648144	29648143	29636803	9	14	6	4689	194	200	11340	197	8014.5	813	1
ENSG00000151532:+:10:112460523:112464657	ENSG00000151532	ENST00000393077,MSTRG.4650.12,MSTRG.4650.14,ENST00000432306,MSTRG.4650.9,MSTRG.4650.3,MSTRG.4650.7,MSTRG.4650.10,ENST00000496445,MSTRG.4650.8,ENST00000489142,ENST00000480057,ENST00000489357	ENST00000393077	8	+	10	112447468	112460523	112460524	112460582	112464547	112464657	112464658	112527086	2	3	2	13055	58	110	62428	84	37741.5	170	1
ENSG00000146463:+:1:35381258:35381758	ENSG00000146463	MSTRG.818.2,MSTRG.818.1,ENST00000314607,MSTRG.818.4,MSTRG.818.8,MSTRG.818.5,MSTRG.818.10,MSTRG.818.6,MSTRG.818.7,ENST00000457946	MSTRG.818.2	32	+	1	35370628	35381258	35381259	35381433	35381546	35381758	35381759	35385441	10	11	2	10630	174	212	3682	193	7156	388	1
ENSG00000230124:-:1:180397515:180430262	ENSG00000230124	ENST00000645415,ENST00000642319,MSTRG.2688.5,MSTRG.2688.18,MSTRG.2688.2,MSTRG.2688.3,MSTRG.2688.20,MSTRG.2688.17,MSTRG.2688.1,MSTRG.2688.25,MSTRG.2688.15,MSTRG.2688.16,MSTRG.2688.9,MSTRG.2688.12,MSTRG.2688.8,MSTRG.2688.10,MSTRG.2688.14,MSTRG.2688.21,MSTRG.2688.13,MSTRG.2688.11,MSTRG.2688.22,MSTRG.2688.26,MSTRG.2688.28,MSTRG.2688.27,MSTRG.2688.30,ENST00000367595,ENST00000496993	ENST00000645415	15	-	1	180492268	180430263	180430262	180430180	180397605	180397516	180397515	180326563	4	6	3	62005	82	89	70952	85.5	66478.5	279	1
ENSG00000115239:-:2:53728711:53729570	ENSG00000115239	ENST00000406053,ENST00000263634,ENST00000406687,ENST00000406625,ENST00000482829,MSTRG.18397.11,ENST00000394717,MSTRG.18397.9,MSTRG.18397.10,ENST00000498475	ENST00000406053	10	-	2	53750782	53729571	53729570	53729458	53728847	53728712	53728711	53716744	3	4	2	21211	112	135	11967	123.5	16589	249	1
ENSG00000159692:-:4:1228198:1228343	ENSG00000159692	ENST00000382952,MSTRG.24434.3,ENST00000290921,MSTRG.24434.6,MSTRG.24434.5,MSTRG.24434.9,MSTRG.24434.15,MSTRG.24434.19,MSTRG.24434.14,MSTRG.24434.8,MSTRG.24434.16,MSTRG.24434.13,MSTRG.24434.17,MSTRG.24434.11,MSTRG.24434.26,MSTRG.24434.10,ENST00000514210,ENST00000515399,ENST00000504092,ENST00000513420,ENST00000506180,ENST00000514669,MSTRG.24434.24,ENST00000505826,ENST00000514495	ENST00000382952	10	-	4	1238182	1228344	1228343	1228199	1228343	1228199	1228198	1225567	4	4	1	9838	144	144	2631	144	6234.5	145	1
ENSG00000188191:-:7:596145:596304	ENSG00000188191	ENST00000544935,ENST00000406797,ENST00000537384,MSTRG.29352.4,ENST00000360274,ENST00000403562,ENST00000430040,ENST00000400758	ENST00000544935	11	-	7	606192	596305	596304	596146	596304	596146	596145	584569	7	7	1	9887	158	158	11576	158	10731.5	159	1
ENSG00000197157:+:7:127887901:127888012	ENSG00000197157	ENST00000354725,MSTRG.30622.1,MSTRG.30622.5,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.6,MSTRG.30622.2,MSTRG.30622.4,ENST00000465900,ENST00000468166	ENST00000354725	24	+	7	127844425	127887901	127887902	127888012	127887902	127888012	127888013	127904746	13	13	1	43476	110	110	16733	110	30104.5	111	1
ENSG00000149269:-:11:77358897:77392538	ENSG00000149269	MSTRG.6033.5,MSTRG.6033.4	MSTRG.6033.5	15	-	11	77473551	77392539	77392538	77392331	77359017	77358898	77358897	77355843	2	6	5	81012	207	119	3054	163	42033	615	1
ENSG00000121073_ENSG00000121104:-:17:49716164:49717714	ENSG00000121073_ENSG00000121104	MSTRG.14643.15,MSTRG.14643.11,MSTRG.14643.26,MSTRG.14643.19,MSTRG.14643.25,MSTRG.14643.27	MSTRG.14643.15	13	-	17	49719759	49717715	49717714	49717513	49716315	49716165	49716164	49707069	5	6	2	2044	201	150	9095	175.5	5569.5	353	1
ENSG00000214331:-:16:74353776:74360891	ENSG00000214331	MSTRG.13017.3,MSTRG.13017.14,MSTRG.13017.17,MSTRG.13017.13,MSTRG.13017.10,ENST00000566802,ENST00000429810,MSTRG.13017.16,MSTRG.13017.15,ENST00000563377	MSTRG.13017.3	17	-	16	74368064	74360892	74360891	74360633	74353910	74353777	74353776	74352379	2	3	2	7172	258	133	1397	195.5	4284.5	393	1
ENSG00000105176:+:19:29985222:30012531	ENSG00000105176	ENST00000392271,ENST00000574110,MSTRG.16897.8,MSTRG.16897.11,MSTRG.16897.6,MSTRG.16897.10,ENST00000360605	ENST00000392271	11	+	19	29971228	29985222	29985223	29985301	30012285	30012531	30012532	30014886	3	10	8	13994	78	246	2354	162	8174	1273	1
ENSG00000175866:+:17:81053667:81086580	ENSG00000175866	ENST00000428708,ENST00000321300,ENST00000575712,MSTRG.15251.10,ENST00000575245,ENST00000572329,ENST00000435091,ENST00000321280,MSTRG.15251.9,MSTRG.15251.2,ENST00000572918,ENST00000573017,ENST00000575750,ENST00000573894,ENST00000575989,ENST00000571530	ENST00000428708	14	+	17	81035309	81053667	81053668	81053743	81086443	81086580	81086581	81099927	2	6	5	18358	75	137	13346	106	15852	435	1
ENSG00000097021:-:1:6294863:6318578	ENSG00000097021	ENST00000418124,ENST00000377860,ENST00000377842,ENST00000608083,ENST00000361521,ENST00000545482,MSTRG.194.9,ENST00000377855,ENST00000377845,ENST00000473466	ENST00000418124	10	-	1	6327298	6318579	6318578	6318492	6294980	6294864	6294863	6282889	6	7	2	8719	86	116	11974	101	10346.5	204	1
ENSG00000004487:+:1:23030468:23059167	ENSG00000004487	MSTRG.523.27,ENST00000687202,ENST00000691256,ENST00000689366,ENST00000688235,ENST00000688122,ENST00000692214,ENST00000688680,ENST00000691404,ENST00000692975,ENST00000691682,ENST00000685102,ENST00000400181,ENST00000465864,ENST00000356634,ENST00000689853,ENST00000691694,ENST00000686934,ENST00000690627,ENST00000692853,MSTRG.523.28,ENST00000686771,ENST00000686270,ENST00000692056,ENST00000693156,ENST00000692209,ENST00000691813,ENST00000686339	MSTRG.523.27	20	+	1	23019948	23030468	23030469	23030634	23059073	23059167	23059168	23068538	2	9	8	10520	165	94	9370	129.5	9945	816	1
ENSG00000090273:+:1:26942659:26943065	ENSG00000090273	MSTRG.630.2	MSTRG.630.2	10	+	1	26941819	26942656	26942657	26942776	26942871	26943065	26943066	26945389	6	7	2	837	119	194	2323	156.5	1580	315	1
ENSG00000155903:+:3:141512162:141540609	ENSG00000155903	MSTRG.23817.1,ENST00000286364,ENST00000515549	MSTRG.23817.1	24	+	3	141487217	141512162	141512163	141512280	141540533	141540609	141540610	141553856	2	5	4	24945	117	76	13246	96.5	19095.5	394	1
ENSG00000158417:+:2:99360235:99368591	ENSG00000158417	MSTRG.18937.2,MSTRG.18937.1,ENST00000617677,MSTRG.18937.4,ENST00000289371,MSTRG.18937.6,MSTRG.18937.5,MSTRG.18937.9,MSTRG.18937.8	MSTRG.18937.2	24	+	2	99337590	99360235	99360236	99360361	99368493	99368591	99368592	99369391	2	7	6	22645	125	98	799	111.5	11722	1352	1
ENSG00000188177:+:2:112299848:112300029	ENSG00000188177	ENST00000409871,ENST00000343936,MSTRG.19047.1	ENST00000409871	12	+	2	112276027	112299848	112299849	112300029	112299849	112300029	112300030	112303228	2	2	1	23821	180	180	3198	180	13509.5	181	1
ENSG00000134910_ENSG00000149554:+:11:125629231:125633351	ENSG00000134910_ENSG00000149554	MSTRG.6529.8,MSTRG.6529.2	MSTRG.6529.8	35	+	11	125627831	125629231	125629232	125629296	125633163	125633351	125633352	125635428	23	25	3	1400	64	188	2076	126	1738	324	1
ENSG00000181090_ENSG00000197070:+:9:137790847:137818138	ENSG00000181090_ENSG00000197070	MSTRG.33733.4,MSTRG.33733.1	MSTRG.33733.4	31	+	9	137782398	137790847	137790848	137790970	137818060	137818138	137818139	137834348	20	29	10	8449	122	78	16209	100	12329	1158	1
ENSG00000075223:-:7:80789305:80828745	ENSG00000075223	ENST00000265361,ENST00000419255	ENST00000265361	18	-	7	80916678	80828746	80828745	80828585	80789528	80789306	80789305	80765244	3	12	10	87932	160	222	24061	191	55996.5	1251	1
ENSG00000143401:-:1:150223185:150229237	ENSG00000143401	MSTRG.2148.1,ENST00000583931,ENST00000616917,MSTRG.2148.10,MSTRG.2148.6,ENST00000436748,MSTRG.2148.11,MSTRG.2148.13	MSTRG.2148.1	8	-	1	150230570	150229238	150229237	150229072	150223240	150223186	150223185	150220762	5	7	3	1332	165	54	2423	109.5	1877.5	409	1
ENSG00000135336:+:6:87656905:87658018	ENSG00000135336	MSTRG.28634.3,ENST00000257789,ENST00000392844,ENST00000546266,MSTRG.28634.5,MSTRG.28634.4	MSTRG.28634.3	21	+	6	87653250	87656902	87656903	87656982	87657921	87658018	87658019	87663002	16	17	2	3652	79	97	4983	88	4317.5	178	1
ENSG00000138814:-:4:101195915:101196116	ENSG00000138814	ENST00000394854,ENST00000323055,ENST00000512215,ENST00000394853,MSTRG.25235.5,ENST00000492351,ENST00000529324,ENST00000525819	ENST00000394854	14	-	4	101346738	101196117	101196116	101195916	101196116	101195916	101195915	101109079	2	2	1	150621	200	200	86836	200	118728.5	201	1
ENSG00000196739:+:9:114162714:114183075	ENSG00000196739	ENST00000356083,MSTRG.33270.2,MSTRG.33270.4,MSTRG.33270.3	ENST00000356083	61	+	9	114156013	114162714	114162715	114162785	114183022	114183075	114183076	114194403	2	5	4	6701	70	53	11327	61.5	9014	1954	1
ENSG00000140612:-:15:84687624:84691644	ENSG00000140612	MSTRG.11445.7,ENST00000455959,ENST00000560266,ENST00000268220,ENST00000558134,ENST00000558217,ENST00000560409,ENST00000558196	MSTRG.11445.7	7	-	15	84714730	84691645	84691644	84691535	84687774	84687625	84687624	84680833	3	4	2	23085	109	149	6791	129	14938	260	1
ENSG00000196365:-:19:5705771:5714271	ENSG00000196365	ENST00000360614,ENST00000540670,ENST00000590558,ENST00000593119,ENST00000585374,ENST00000587552,ENST00000590729	ENST00000360614	18	-	19	5719703	5714272	5714271	5714183	5705992	5705772	5705771	5700928	2	8	7	5431	88	220	4843	154	5137	938	1
ENSG00000186660:+:11:58610297:58611738	ENSG00000186660	ENST00000316059	ENST00000316059	11	+	11	58610040	58610297	58610298	58610334	58611604	58611738	58611739	58612277	4	6	3	257	36	134	538	85	397.5	277	1
ENSG00000186716:+:22:23271531:23273774	ENSG00000186716	ENST00000359540,ENST00000305877,MSTRG.21779.4,MSTRG.21779.5,ENST00000427791	ENST00000359540	22	+	22	23268516	23271531	23271532	23271592	23273634	23273774	23273775	23283976	6	8	3	3015	60	140	10201	100	6608	255	1
ENSG00000140332:-:15:70074502:70076158	ENSG00000140332	ENST00000559929,ENST00000654081	ENST00000559929	20	-	15	70094531	70076159	70076158	70076096	70074607	70074503	70074502	70066219	5	6	2	18372	62	104	8283	83	13327.5	168	1
ENSG00000151292:+:5:123545416:123545841	ENSG00000151292	MSTRG.26826.1,MSTRG.26826.3,MSTRG.26826.2,ENST00000345990,MSTRG.26826.4,MSTRG.26826.7,MSTRG.26826.10,MSTRG.26826.11,ENST00000510842,ENST00000521364	MSTRG.26826.1	12	+	5	123512571	123545416	123545417	123545841	123545417	123545841	123545842	123553106	2	2	1	32845	424	424	7264	424	20054.5	425	1
ENSG00000134775:+:18:36501931:36512543	ENSG00000134775	ENST00000590592,ENST00000257209,MSTRG.15710.1,ENST00000359247,MSTRG.15710.6,MSTRG.15710.2,MSTRG.15710.8,ENST00000589114,MSTRG.15710.7	ENST00000590592	29	+	18	36372745	36501931	36501932	36501999	36512438	36512543	36512544	36576450	4	5	2	129186	67	105	63906	86	96546	174	1
ENSG00000065526:+:1:15909323:15936266	ENSG00000065526	MSTRG.376.9,ENST00000375759,MSTRG.376.10,MSTRG.376.4,MSTRG.376.6,MSTRG.376.3,MSTRG.376.5	MSTRG.376.9	14	+	1	15876679	15909320	15909321	15909481	15928091	15936266	15936267	15937162	3	10	8	32641	160	8175	895	4167.5	16768	9145	1
ENSG00000075151:-:1:20941490:21111383	ENSG00000075151	MSTRG.488.20,MSTRG.488.27,ENST00000356916,ENST00000682284	MSTRG.488.20	36	-	1	21176721	21111384	21111383	21111323	20942330	20941491	20941490	20904972	2	12	11	65337	60	839	36518	449.5	50927.5	1941	1
ENSG00000134255:+:1:111147641:111148053	ENSG00000134255	MSTRG.1799.1,MSTRG.1799.7,ENST00000498239,ENST00000357172,MSTRG.1799.5,ENST00000478042,ENST00000480324,ENST00000476865,ENST00000460443	MSTRG.1799.1	10	+	1	111139762	111147641	111147642	111148053	111147642	111148053	111148054	111159379	2	2	1	7879	411	411	11325	411	9602	412	1
ENSG00000197157:+:7:127721286:127807573	ENSG00000197157	ENST00000354725,MSTRG.30622.1,MSTRG.30622.5,MSTRG.30622.3,MSTRG.30622.7,ENST00000465900,ENST00000468166	ENST00000354725	24	+	7	127707648	127721286	127721287	127721400	127807484	127807573	127807574	127844323	10	11	2	13638	113	89	36749	101	25193.5	204	1
ENSG00000125266:-:13:106512528:106512812	ENSG00000125266	ENST00000646441,MSTRG.9043.1	ENST00000646441	5	-	13	106534842	106512813	106512812	106512529	106512812	106512529	106512528	106495841	2	2	1	22029	283	283	16687	283	19358	284	1
ENSG00000271793:-:6:85663673:85666147	ENSG00000271793	ENST00000682106	ENST00000682106	44	-	6	85677423	85666148	85666147	85666004	85663726	85663674	85663673	85641452	4	5	2	11275	143	52	22221	97.5	16748	197	1
ENSG00000162337:+:11:68347846:68365702	ENSG00000162337	MSTRG.5853.1,MSTRG.5853.4,ENST00000294304,ENST00000529993	MSTRG.5853.1	24	+	11	68312806	68347846	68347847	68348243	68365571	68365702	68365703	68386315	2	5	4	35040	396	131	20612	263.5	27826	924	1
ENSG00000115677:-:2:241238648:241249980	ENSG00000115677	ENST00000310931,ENST00000391975,ENST00000427183,MSTRG.20183.4,ENST00000391976,MSTRG.20183.6,MSTRG.20183.9,MSTRG.20183.14,MSTRG.20183.13,ENST00000373292	ENST00000310931	28	-	2	241252956	241249981	241249980	241249841	241238787	241238649	241238648	241236770	12	20	9	2975	139	138	1878	138.5	2426.5	1377	1
ENSG00000204764:+:5:170892386:171170203	ENSG00000204764	ENST00000389118,ENST00000522066,ENST00000519949,ENST00000520864,ENST00000522533,ENST00000523189,ENST00000519256	ENST00000389118	30	+	5	170881897	170892386	170892387	170892553	171170130	171170203	171170204	171171205	4	16	13	10489	166	73	1001	119.5	5745	1646	1
ENSG00000138802:+:4:109462900:109473186	ENSG00000138802	ENST00000504968,ENST00000265175,ENST00000399100,MSTRG.25299.4,MSTRG.25299.5	ENST00000504968	25	+	4	109449510	109462900	109462901	109463644	109473004	109473186	109473187	109481676	3	4	2	13390	743	182	8489	462.5	10939.5	927	1
ENSG00000147677:-:8:116655855:116656005	ENSG00000147677	ENST00000521861,ENST00000276682,MSTRG.32012.2,ENST00000518949,ENST00000520289	ENST00000521861	8	-	8	116657214	116656006	116656005	116655856	116656005	116655856	116655855	116648927	5	5	1	1208	149	149	6928	149	4068	150	1
ENSG00000141551:-:17:82252433:82253244	ENSG00000141551	ENST00000581241,ENST00000314028,MSTRG.15343.9,MSTRG.15343.19,MSTRG.15343.15,ENST00000392334,ENST00000398519,ENST00000578904,ENST00000269361,ENST00000582844	ENST00000581241	8	-	17	82265685	82253245	82253244	82253016	82252604	82252434	82252433	82251528	3	4	2	12440	228	170	905	199	6672.5	400	1
ENSG00000124164:+:20:58418210:58418363	ENSG00000124164	ENST00000475243,ENST00000265619,MSTRG.20904.3,MSTRG.20904.4,ENST00000520497,ENST00000395802	ENST00000475243	6	+	20	58389518	58418210	58418211	58418363	58418211	58418363	58418364	58434601	2	2	1	28692	152	152	16237	152	22464.5	153	1
ENSG00000131697:-:1:5961793:5986327	ENSG00000131697	ENST00000489180,ENST00000378156,MSTRG.186.8,ENST00000378169,ENST00000622020	ENST00000489180	33	-	1	5992243	5986328	5986327	5986155	5961949	5961794	5961793	5952837	2	6	5	5915	172	155	8956	163.5	7435.5	711	1
ENSG00000197157:+:7:127929187:127991056	ENSG00000197157	ENST00000354725,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.2,MSTRG.30622.4,MSTRG.30622.17,MSTRG.30622.18,ENST00000486037,ENST00000492840	ENST00000354725	24	+	7	127904820	127929187	127929188	127929329	127990947	127991056	127991057	128074501	15	16	2	24367	141	109	83444	125	53905.5	252	1
ENSG00000141337:+:17:68306942:68401450	ENSG00000141337	ENST00000448504,ENST00000621439,MSTRG.14992.4,MSTRG.14992.1	ENST00000448504	12	+	17	68259427	68306942	68306943	68307711	68401360	68401450	68401451	68420188	2	11	10	47515	768	90	18737	429	33126	1854	1
ENSG00000141376:+:17:60808003:61084564	ENSG00000141376	ENST00000589222,MSTRG.14829.2,MSTRG.14829.6,ENST00000588462,ENST00000390652,ENST00000407086,ENST00000408905	ENST00000589222	26	+	17	60747280	60808003	60808004	60808076	61084467	61084564	61084565	61368326	7	22	16	60723	72	97	283761	84.5	172242	2022	1
ENSG00000182771:-:10:85613406:85916239	ENSG00000182771	ENST00000327946,MSTRG.4288.2,ENST00000464741	ENST00000327946	16	-	10	86138818	85916240	85916239	85916186	85613647	85613407	85613406	85602702	5	15	11	222578	53	240	10704	146.5	116641	1875	1
ENSG00000184465_ENSG00000272848:-:6:169632946:169638660	ENSG00000184465_ENSG00000272848	MSTRG.29337.7	MSTRG.29337.7	27	-	6	169643696	169638661	169638660	169638539	169633068	169632947	169632946	169602322	20	22	3	5035	121	121	30624	121	17829.5	342	1
ENSG00000151233:-:12:42097441:42119171	ENSG00000151233	ENST00000398675,MSTRG.7122.2,MSTRG.7122.3,MSTRG.7122.4,MSTRG.7122.5,ENST00000280876	ENST00000398675	8	-	12	42129758	42119172	42119171	42119000	42097614	42097442	42097441	42087948	3	7	5	10586	171	172	9493	171.5	10039.5	847	1
ENSG00000144354:+:2:173358711:173359491	ENSG00000144354	MSTRG.19509.2,ENST00000695912,ENST00000306721,MSTRG.19509.6,ENST00000410101,ENST00000435616	MSTRG.19509.2	11	+	2	173354985	173358711	173358712	173358837	173359255	173359491	173359492	173363225	2	3	2	3726	125	236	3733	180.5	3729.5	363	1
ENSG00000158019:+:2:28025225:28129380	ENSG00000158019	ENST00000344773,MSTRG.18137.2,MSTRG.18137.6,ENST00000379632,ENST00000361704,ENST00000342045,ENST00000379624,MSTRG.18137.3,MSTRG.18137.1,ENST00000379629	ENST00000344773	13	+	2	27988088	28025225	28025226	28025420	28129271	28129380	28129381	28237201	5	7	3	37137	194	109	107820	151.5	72478.5	380	1
ENSG00000153107:-:2:111831285:111838512	ENSG00000153107	ENST00000341068,MSTRG.19062.115,MSTRG.19062.117,ENST00000427997	ENST00000341068	48	-	2	111843411	111838513	111838512	111838438	111831434	111831286	111831285	111825856	18	21	4	4898	74	148	5429	111	5163.5	585	1
ENSG00000128683:+:2:170836792:170854022	ENSG00000128683	ENST00000493875,ENST00000625689,MSTRG.19471.6,ENST00000358196,ENST00000414527	ENST00000493875	17	+	2	170831193	170836792	170836793	170836883	170853873	170854022	170854023	170857017	5	14	10	5599	90	149	2994	119.5	4296.5	978	1
ENSG00000081760:+:12:125102678:125107268	ENSG00000081760	ENST00000316519,MSTRG.8295.1,MSTRG.8295.4,MSTRG.8295.6,MSTRG.8295.7,MSTRG.8295.5,ENST00000418937,ENST00000537564,ENST00000537477	ENST00000316519	18	+	12	125091524	125102678	125102679	125102793	125107121	125107268	125107269	125114476	6	8	3	11154	114	147	7207	130.5	9180.5	345	1
ENSG00000071127:-:4:10097710:10103986	ENSG00000071127	ENST00000499869,ENST00000382452,ENST00000508079,ENST00000505851,ENST00000264785	ENST00000499869	15	-	4	10116112	10103987	10103986	10103896	10097891	10097711	10097710	10088742	3	5	3	12125	90	180	8968	135	10546.5	420	1
ENSG00000185842:+:1:225264196:225290082	ENSG00000185842	ENST00000682510,ENST00000430092,ENST00000439375,ENST00000327794,ENST00000638635	ENST00000682510	86	+	1	225259254	225264196	225264197	225264261	225289885	225290082	225290083	225300868	47	55	9	4942	64	197	10785	130.5	7863.5	1312	1
ENSG00000138095:-:2:43918024:43918398	ENSG00000138095	ENST00000684743,ENST00000683802,ENST00000260665,ENST00000683645,ENST00000683459,ENST00000683329,ENST00000682585,ENST00000682308,ENST00000683125,ENST00000684306,ENST00000683072,ENST00000684619,ENST00000683220,ENST00000682546,ENST00000683213,ENST00000683994,ENST00000683796,ENST00000683623,ENST00000683833,MSTRG.18303.16,ENST00000682595,ENST00000682779,ENST00000682933,MSTRG.18303.38,ENST00000683346,ENST00000682885,ENST00000682303,ENST00000682480,ENST00000684341,ENST00000684383,ENST00000684290,ENST00000682607,ENST00000682845,ENST00000683080	ENST00000684743	24	-	2	43925066	43918399	43918398	43918256	43918133	43918025	43918024	43912559	15	16	2	6667	142	108	5465	125	6066	252	1
ENSG00000133424:-:22:33316084:33337801	ENSG00000133424	ENST00000610186,ENST00000608642,ENST00000609799,ENST00000674543,ENST00000397394,ENST00000354992,ENST00000413114,ENST00000676070,ENST00000676370,ENST00000676132,ENST00000675416,ENST00000676031,ENST00000674999,ENST00000674668,ENST00000674816,ENST00000676126,ENST00000674708,ENST00000674780,ENST00000674789,ENST00000675277,ENST00000421768	ENST00000610186	13	-	22	33381918	33337802	33337801	33337646	33316248	33316085	33316084	33304508	9	10	2	44116	155	163	11576	159	27846	320	1
ENSG00000172340:-:3:67495797:67508903	ENSG00000172340	ENST00000307227,ENST00000493112,ENST00000492795	ENST00000307227	11	-	3	67518246	67508904	67508903	67508807	67495940	67495798	67495797	67400852	7	9	3	9342	96	142	94945	119	52143.5	402	1
ENSG00000175470:+:10:131934457:131947791	ENSG00000175470	ENST00000470416,ENST00000455566,MSTRG.4826.7,MSTRG.4826.8,MSTRG.4826.22,MSTRG.4826.4,MSTRG.4826.15,MSTRG.4826.19,MSTRG.4826.24,MSTRG.4826.9,MSTRG.4826.5,MSTRG.4826.21,ENST00000616467,ENST00000482010,MSTRG.4826.12,MSTRG.4826.20,MSTRG.4826.17	ENST00000470416	9	+	10	131912599	131934457	131934458	131934555	131947530	131947791	131947792	131955683	3	8	6	21858	97	261	7891	179	14874.5	982	1
ENSG00000100629:-:14:80743074:80862873	ENSG00000100629	ENST00000555265,ENST00000281129,MSTRG.10021.2	ENST00000555265	25	-	14	80895717	80862874	80862873	80862757	80743267	80743075	80743074	80580424	9	19	11	32843	116	192	162650	154	97746.5	2161	1
ENSG00000141027:-:17:16108785:16126209	ENSG00000141027	ENST00000268712,MSTRG.13733.2,MSTRG.13733.4,MSTRG.13733.5,MSTRG.13733.6,MSTRG.13733.7,MSTRG.13733.3,MSTRG.13733.8,MSTRG.13733.17,MSTRG.13733.13,MSTRG.13733.15,MSTRG.13733.16,ENST00000395851,MSTRG.13733.12,MSTRG.13733.22,MSTRG.13733.20,MSTRG.13733.19,MSTRG.13733.21,MSTRG.13733.32,MSTRG.13733.33,MSTRG.13733.31,ENST00000395848	ENST00000268712	46	-	17	16137310	16126207	16126206	16126082	16108912	16108786	16108785	16101758	15	19	5	11103	124	126	7027	125	9065	673	1
ENSG00000145743:-:5:108348398:108348530	ENSG00000145743	ENST00000542267,ENST00000496714,ENST00000481160	ENST00000542267	9	-	5	108364737	108348531	108348530	108348399	108348530	108348399	108348398	108224229	4	4	1	16206	131	131	124169	131	70187.5	132	1
ENSG00000177302:-:17:18285141:18306966	ENSG00000177302	ENST00000321105,ENST00000542570,MSTRG.13793.9,ENST00000584582,MSTRG.13793.1,MSTRG.13793.5,ENST00000582981,ENST00000580095,MSTRG.13793.4	ENST00000321105	19	-	17	18308350	18306967	18306966	18306891	18285307	18285142	18285141	18282842	4	15	12	1383	75	165	2299	120	1841	1563	1
ENSG00000197312:+:1:15638306:15643650	ENSG00000197312	ENST00000480945,MSTRG.365.4	ENST00000480945	10	+	1	15633566	15638306	15638307	15638434	15643522	15643650	15643651	15649719	5	6	2	4740	127	128	6068	127.5	5404	257	1
ENSG00000106976:+:9:128218230:128222860	ENSG00000106976	ENST00000627543,ENST00000628346,ENST00000475805,ENST00000341179,ENST00000393594,MSTRG.33445.8,MSTRG.33445.6,MSTRG.33445.16,ENST00000372923,MSTRG.33445.11,MSTRG.33445.10,MSTRG.33445.15,ENST00000486160,ENST00000634267,ENST00000627061,MSTRG.33445.13	ENST00000627543	22	+	9	128203632	128218230	128218231	128218304	128222793	128222860	128222861	128224250	2	9	8	14598	73	67	1389	70	7993.5	1035	1
ENSG00000067177:-:X:72666150:72667473	ENSG00000067177	ENST00000373542,ENST00000339490,ENST00000373545,ENST00000541944,MSTRG.34446.5,ENST00000373539	ENST00000373542	32	-	X	72676069	72667474	72667473	72667375	72666297	72666151	72666150	72657642	7	8	2	8595	98	146	8508	122	8551.5	246	1
ENSG00000124784:+:6:7411331:7414390	ENSG00000124784	MSTRG.27608.2,MSTRG.27608.1,ENST00000379834,ENST00000264874	MSTRG.27608.2	17	+	6	7410452	7411331	7411332	7411451	7414254	7414390	7414391	7417330	14	16	3	879	119	136	2939	127.5	1909	311	1
ENSG00000108094:-:10:35025131:35033273	ENSG00000108094	ENST00000688044,ENST00000688252,ENST00000374748,ENST00000537177,ENST00000374749,ENST00000374751,ENST00000626172,ENST00000690123,ENST00000374746,ENST00000692866,ENST00000687282,ENST00000688390,ENST00000691457,ENST00000691974,ENST00000688571,ENST00000673636,ENST00000690393,ENST00000693739,ENST00000374754,ENST00000687194,ENST00000685681,MSTRG.3809.13,ENST00000691962,MSTRG.3809.19,ENST00000691858,ENST00000691263,ENST00000691272,ENST00000687524,ENST00000691201,ENST00000421317,ENST00000690092,ENST00000684965,ENST00000688541,ENST00000689330	ENST00000688044	11	-	10	35035171	35033274	35033273	35033166	35025198	35025132	35025131	35016395	2	7	6	1897	107	66	8736	86.5	5316.5	1497	1
ENSG00000144224:+:2:135769768:135770735	ENSG00000144224	MSTRG.19269.2,ENST00000272638,ENST00000490163,MSTRG.19269.1	MSTRG.19269.2	14	+	2	135761912	135769768	135769769	135769823	135770571	135770735	135770736	135772419	7	8	2	7856	54	164	1683	109	4769.5	220	1
ENSG00000120306:+:5:140194445:140194652	ENSG00000120306	MSTRG.27034.6,ENST00000261811,ENST00000509589,ENST00000504227,ENST00000644078	MSTRG.27034.6	3	+	5	140176245	140194445	140194446	140194652	140194446	140194652	140194653	140243304	2	2	1	18200	206	206	48651	206	33425.5	207	1
ENSG00000104375:-:8:98767242:98774819	ENSG00000104375	ENST00000523601,ENST00000419617,ENST00000617590,ENST00000424861,ENST00000518165,ENST00000521649	ENST00000523601	13	-	8	98883646	98774820	98774819	98774739	98767371	98767243	98767242	98749391	4	5	2	108826	80	128	17851	104	63338.5	210	1
ENSG00000063046:+:12:53016472:53022627	ENSG00000063046	MSTRG.7336.2,MSTRG.7336.3,MSTRG.7336.14,ENST00000420463,ENST00000262056,ENST00000416762,ENST00000550704,ENST00000549077,ENST00000550390,ENST00000552490	MSTRG.7336.2	16	+	12	53008644	53016472	53016473	53016610	53022493	53022627	53022628	53027781	3	7	5	7828	137	134	5153	135.5	6490.5	654	1
ENSG00000175029:-:10:125038996:125039155	ENSG00000175029	MSTRG.4789.1,ENST00000337195,MSTRG.4789.4,MSTRG.4789.8,MSTRG.4789.7,MSTRG.4789.5,MSTRG.4789.6,MSTRG.4789.9,MSTRG.4789.13,MSTRG.4789.20,ENST00000411419,MSTRG.4789.10,MSTRG.4789.12,MSTRG.4789.11,ENST00000494626,ENST00000531469,MSTRG.4789.17,ENST00000476817,ENST00000460976	MSTRG.4789.1	12	-	10	125110989	125039156	125039155	125038997	125039155	125038997	125038996	125003493	4	4	1	71833	158	158	35503	158	53668	159	1
ENSG00000157985:+:2:236049058:236049281	ENSG00000157985	ENST00000409538,ENST00000304032,ENST00000336665,ENST00000428334,ENST00000635100,ENST00000614409,ENST00000453371,MSTRG.20106.4,ENST00000418654,ENST00000466575	ENST00000409538	17	+	2	236040842	236049058	236049059	236049281	236049059	236049281	236049282	236120191	15	15	1	8216	222	222	70909	222	39562.5	223	1
ENSG00000102024:+:X:115629834:115643508	ENSG00000102024	ENST00000481823,MSTRG.34749.1,MSTRG.34749.4,ENST00000289290,ENST00000355899,ENST00000539310	ENST00000481823	17	+	X	115629328	115629834	115629835	115629967	115643313	115643508	115643509	115645020	6	11	6	506	132	195	1511	163.5	1008.5	816	1
ENSG00000164587:-:5:150446801:150446963	ENSG00000164587	ENST00000407193,ENST00000401695,ENST00000521466,MSTRG.27163.8,ENST00000312037,MSTRG.27163.3,ENST00000518139,ENST00000642507,MSTRG.27163.13,ENST00000519855	ENST00000407193	5	-	5	150447584	150446964	150446963	150446802	150446963	150446802	150446801	150445686	3	3	1	620	161	161	1115	161	867.5	162	1
ENSG00000197157:+:7:127807483:127807573	ENSG00000197157	ENST00000354725,MSTRG.30622.1,MSTRG.30622.5,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.2,MSTRG.30622.4,ENST00000465900,ENST00000468166	ENST00000354725	24	+	7	127721401	127807483	127807484	127807573	127807484	127807573	127807574	127844323	11	11	1	86082	89	89	36749	89	61415.5	90	1
ENSG00000095209:+:9:105748072:105748190	ENSG00000095209	ENST00000374688,MSTRG.33187.9,MSTRG.33187.2,MSTRG.33187.7,MSTRG.33187.3,ENST00000374692,MSTRG.33187.5,MSTRG.33187.11,ENST00000435034,ENST00000451560	ENST00000374688	6	+	9	105722622	105748072	105748073	105748190	105748073	105748190	105748191	105773864	5	5	1	25450	117	117	25673	117	25561.5	118	1
ENSG00000078304:+:14:101856685:101856885	ENSG00000078304	ENST00000694906,ENST00000422945,ENST00000328724,ENST00000334743,ENST00000350249,MSTRG.10194.10,MSTRG.10194.8,MSTRG.10194.23,ENST00000445439,MSTRG.10194.19,ENST00000557095,ENST00000553730,ENST00000557621,ENST00000556307,ENST00000556493,ENST00000556373	ENST00000694906	16	+	14	101786184	101856685	101856686	101856885	101856686	101856885	101856886	101882160	4	4	1	70501	199	199	25274	199	47887.5	200	1
ENSG00000198589:-:4:151014426:151014861	ENSG00000198589	ENST00000357115,ENST00000651943,MSTRG.25594.4,ENST00000510413,MSTRG.25594.7,MSTRG.25594.11,ENST00000651035,MSTRG.25594.15,ENST00000507224	ENST00000357115	58	-	4	151015472	151014862	151014861	151014427	151014861	151014427	151014426	150929066	2	2	1	610	434	434	85360	434	42985	435	1
ENSG00000115286:+:19:1383707:1383942	ENSG00000115286	MSTRG.16055.1,MSTRG.16055.3,ENST00000546283	MSTRG.16055.1	9	+	19	1383609	1383707	1383708	1383942	1383708	1383942	1383943	1387810	2	2	1	98	234	234	3867	234	1982.5	235	1
ENSG00000107643:+:10:48401611:48410168	ENSG00000107643	MSTRG.3948.2,ENST00000374179,MSTRG.3948.4,MSTRG.3948.9,MSTRG.3948.5,ENST00000395611,ENST00000374189,MSTRG.3948.8,ENST00000360332,ENST00000432379,ENST00000426557,ENST00000429041	MSTRG.3948.2	14	+	10	48325966	48401611	48401612	48401782	48410030	48410168	48410169	48420154	4	7	4	75645	170	138	9985	154	42815	499	1
ENSG00000138363:+:2:215318156:215326138	ENSG00000138363	ENST00000435675,ENST00000443953,ENST00000236959,MSTRG.19922.1,ENST00000427397,ENST00000488712,ENST00000444305	ENST00000435675	15	+	2	215312625	215318156	215318157	215318233	215325987	215326138	215326139	215326821	2	5	4	5531	76	151	682	113.5	3106.5	385	1
ENSG00000106070:-:7:50669721:50703908	ENSG00000106070	ENST00000644769,ENST00000401949,MSTRG.29861.1,ENST00000335866,ENST00000403097,ENST00000398810,ENST00000398812,MSTRG.29861.22,MSTRG.29861.15,MSTRG.29861.18,MSTRG.29861.14,ENST00000407526,ENST00000406641,MSTRG.29861.20,MSTRG.29861.19,ENST00000643299,MSTRG.29861.23,MSTRG.29861.28,MSTRG.29861.25,MSTRG.29861.26,ENST00000402497,MSTRG.29861.24,MSTRG.29861.17,MSTRG.29861.16,ENST00000645075,ENST00000357271	ENST00000644769	18	-	7	50710874	50703909	50703908	50703821	50669863	50669722	50669721	50626979	4	6	3	6965	87	141	42742	114	24853.5	453	1
ENSG00000124795:-:6:18249650:18249839	ENSG00000124795	ENST00000652689,ENST00000507591,ENST00000515770,ENST00000651992,MSTRG.27700.2,MSTRG.27700.5,MSTRG.27700.8,ENST00000651624,MSTRG.27700.11,MSTRG.27700.12,ENST00000244776,ENST00000505224,ENST00000652576,ENST00000512145	ENST00000652689	11	-	6	18255730	18249840	18249839	18249651	18249839	18249651	18249650	18237517	7	7	1	5890	188	188	12133	188	9011.5	189	1
ENSG00000187109:-:12:76059797:76059878	ENSG00000187109	ENST00000618691,MSTRG.7677.2,ENST00000552056,ENST00000547773,MSTRG.7677.4,ENST00000393263,ENST00000549596,ENST00000535020,ENST00000542344,ENST00000544816,ENST00000552342,ENST00000548044,ENST00000551992,ENST00000552147,ENST00000547529,ENST00000548273,ENST00000551524	ENST00000618691	15	-	12	76060137	76059879	76059878	76059798	76059878	76059798	76059797	76056162	6	6	1	258	80	80	3635	80	1946.5	81	1
ENSG00000110328:-:11:11327085:11341004	ENSG00000110328	ENST00000227756,MSTRG.5164.1	ENST00000227756	11	-	11	11372514	11341005	11341004	11340819	11327181	11327086	11327085	11293194	7	9	3	31509	185	95	33891	140	32700	420	1
ENSG00000110958:-:12:56670274:56670364	ENSG00000110958	ENST00000537473,ENST00000614328,ENST00000262033,MSTRG.7477.5,MSTRG.7477.8,MSTRG.7477.11,MSTRG.7477.12,ENST00000436399,ENST00000448157,MSTRG.7477.16,ENST00000456859	ENST00000537473	8	-	12	56671748	56670365	56670364	56670275	56670364	56670275	56670274	56666267	5	5	1	1383	89	89	4007	89	2695	90	1
ENSG00000141556:+:17:82797756:82884202	ENSG00000141556	ENST00000681983,ENST00000684361,ENST00000684760,ENST00000684349,ENST00000683444,ENST00000682479,ENST00000684464,ENST00000684429,ENST00000539345,ENST00000355528,ENST00000682722,ENST00000684000,ENST00000683041,ENST00000684544,ENST00000683282,ENST00000683184,ENST00000684188,ENST00000684776,ENST00000682213,ENST00000684408,ENST00000683009,ENST00000682921	ENST00000681983	38	+	17	82781722	82797756	82797757	82797802	82884145	82884202	82884203	82889667	8	15	8	16034	45	57	5464	51	10749	762	1
ENSG00000254536:+:10:133398434:133402773	ENSG00000254536	ENST00000468317	ENST00000468317	16	+	10	133396268	133398434	133398435	133398515	133402692	133402773	133402774	133419479	9	14	6	2166	80	81	16705	80.5	9435.5	470	1
ENSG00000131747:-:17:40400208:40411455	ENSG00000131747	ENST00000423485,MSTRG.14296.3	ENST00000423485	35	-	17	40411644	40411456	40411455	40411354	40400409	40400209	40400208	40400068	9	23	15	188	101	200	140	150.5	164	2037	1
ENSG00000182446:-:17:81613317:81613494	ENSG00000182446	ENST00000374747,ENST00000331134,ENST00000574897,ENST00000574344,ENST00000570300,ENST00000570324,ENST00000571562,ENST00000625705	ENST00000374747	16	-	17	81622165	81613495	81613494	81613318	81613494	81613318	81613317	81610259	4	4	1	8670	176	176	3058	176	5864	177	1
ENSG00000165916:-:11:47425866:47425946	ENSG00000165916	MSTRG.5472.5,ENST00000530651,ENST00000527906	MSTRG.5472.5	12	-	11	47426116	47425947	47425946	47425867	47425946	47425867	47425866	47425247	2	2	1	169	79	79	619	79	394	80	1
ENSG00000112308:-:6:24708776:24716324	ENSG00000112308	ENST00000378119,MSTRG.27734.2,MSTRG.27734.3,ENST00000378102	ENST00000378119	5	-	6	24718539	24716325	24716324	24716148	24708911	24708777	24708776	24706263	2	4	3	2214	176	134	2513	155	2363.5	435	1
ENSG00000122566:-:7:26193250:26193694	ENSG00000122566	ENST00000463181,ENST00000490912,ENST00000677075,ENST00000678075,ENST00000678035,ENST00000356674,ENST00000677839,ENST00000676932,ENST00000354667,ENST00000618183,ENST00000679021,ENST00000677906,ENST00000677669,ENST00000679123,ENST00000677574,ENST00000678277,ENST00000679124,ENST00000677571,ENST00000678962,ENST00000678501,ENST00000679001,ENST00000678449,ENST00000677339,ENST00000608362,ENST00000679243,ENST00000678431,ENST00000677656,ENST00000676903,ENST00000677631,ENST00000678675,ENST00000676749,ENST00000676524,ENST00000676497,MSTRG.29609.62,ENST00000676746,ENST00000678935,ENST00000678998,ENST00000677396,ENST00000678697,ENST00000678779,MSTRG.29609.65,MSTRG.29609.30,ENST00000360787,MSTRG.29609.70,ENST00000679318,ENST00000677037,ENST00000678884,MSTRG.29609.50,MSTRG.29609.66,MSTRG.29609.72,MSTRG.29609.69,MSTRG.29609.52,MSTRG.29609.53,MSTRG.29609.71,MSTRG.29609.55,MSTRG.29609.11,MSTRG.29609.63	ENST00000463181	9	-	7	26195846	26193695	26193694	26193575	26193373	26193251	26193250	26192578	7	8	2	2151	119	122	672	120.5	1411.5	243	1
ENSG00000166225:+:12:69530864:69532056	ENSG00000166225	MSTRG.7651.2,ENST00000549921,MSTRG.7651.7,MSTRG.7651.11,ENST00000550937,ENST00000547219,ENST00000549092,ENST00000548154,ENST00000550169,ENST00000550316,ENST00000547414	MSTRG.7651.2	10	+	12	69486298	69530864	69530865	69530960	69532014	69532056	69532057	69562179	3	4	2	44566	95	42	30122	68.5	37344	139	1
ENSG00000277149:-:7:72694686:72713798	ENSG00000277149	ENST00000620995,MSTRG.29992.3,ENST00000612372	ENST00000620995	14	-	7	72728821	72713799	72713798	72713621	72694822	72694687	72694686	72628998	10	11	2	15022	177	135	65688	156	40355	314	1
ENSG00000055130:+:7:148729961:148730283	ENSG00000055130	ENST00000671421,MSTRG.30880.15	ENST00000671421	24	+	7	148699030	148729961	148729962	148730283	148729962	148730283	148730284	148737621	2	2	1	30931	321	321	7337	321	19134	322	1
ENSG00000067798:+:12:77571858:77572266	ENSG00000067798	ENST00000550042	ENST00000550042	9	+	12	77325215	77571858	77571859	77572266	77571859	77572266	77572267	77940318	2	2	1	246643	407	407	368051	407	307347	408	1
ENSG00000136938:+:9:98011273:98011389	ENSG00000136938	MSTRG.33115.2,MSTRG.33115.4,MSTRG.33115.6,MSTRG.33115.5,MSTRG.33115.9,ENST00000339399,MSTRG.33115.3,MSTRG.33115.7,MSTRG.33115.8,MSTRG.33115.10,MSTRG.33115.12,MSTRG.33115.15,MSTRG.33115.18,MSTRG.33115.17	MSTRG.33115.2	7	+	9	98005154	98011273	98011274	98011389	98011274	98011389	98011390	98012420	5	5	1	6119	115	115	1030	115	3574.5	116	1
ENSG00000067225:-:15:72217408:72219110	ENSG00000067225	MSTRG.11164.7,MSTRG.11164.1,ENST00000319622,ENST00000565184,ENST00000335181,MSTRG.11164.10,ENST00000565154,ENST00000568459,ENST00000566809,ENST00000561609,ENST00000567087,ENST00000569857,ENST00000564276,ENST00000562997,ENST00000564178	MSTRG.11164.7	11	-	15	72231115	72219111	72219110	72218944	72217500	72217409	72217408	72210479	2	3	2	12004	166	91	6929	128.5	9466.5	259	1
ENSG00000135624:+:2:73239642:73250438	ENSG00000135624	MSTRG.18661.3,ENST00000539919,ENST00000258091,MSTRG.18661.7,MSTRG.18661.13,MSTRG.18661.6,MSTRG.18661.22,MSTRG.18661.11,MSTRG.18661.10,MSTRG.18661.23	MSTRG.18661.3	12	+	2	73234385	73239642	73239643	73239796	73250306	73250438	73250439	73251225	2	10	9	5257	153	132	786	142.5	3021.5	1201	1
ENSG00000184575:+:12:64409961:64430287	ENSG00000184575	ENST00000332707,MSTRG.7579.2,MSTRG.7579.4	ENST00000332707	25	+	12	64404805	64409961	64409962	64410095	64430049	64430287	64430288	64431537	2	17	16	5156	133	238	1249	185.5	3202.5	2050	1
ENSG00000114316:-:3:49276426:49327816	ENSG00000114316	MSTRG.22947.1,MSTRG.22947.5,MSTRG.22947.6,MSTRG.22947.7,MSTRG.22947.8	MSTRG.22947.1	24	-	3	49335468	49327817	49327816	49327686	49276841	49276427	49276426	49274141	3	22	20	7651	130	414	2285	272	4968	3151	1
ENSG00000142856:-:1:63478763:63508570	ENSG00000142856	MSTRG.1337.12,ENST00000371092,ENST00000460251,ENST00000489099,ENST00000271002,MSTRG.1337.2,MSTRG.1337.1,ENST00000460394,MSTRG.1337.14	MSTRG.1337.12	8	-	1	63523128	63508571	63508570	63508528	63478833	63478764	63478763	63454969	2	4	3	14557	42	69	23794	55.5	19175.5	249	1
ENSG00000136813:-:9:111386376:111391824	ENSG00000136813	ENST00000259335,ENST00000684092,ENST00000338205,MSTRG.33224.5,MSTRG.33224.11,MSTRG.33224.9,MSTRG.33224.7,MSTRG.33224.4,MSTRG.33224.10,MSTRG.33224.8	ENST00000259335	51	-	9	111392767	111391825	111391824	111391756	111386456	111386377	111386376	111385443	30	33	4	942	68	79	933	73.5	937.5	435	1
ENSG00000130414:-:2:239990073:240007370	ENSG00000130414	ENST00000678188,ENST00000678562,ENST00000476216,ENST00000677395,ENST00000678468,ENST00000677764,ENST00000677979,MSTRG.20164.20,ENST00000252711,ENST00000678455,ENST00000678832,ENST00000678914,ENST00000676784,ENST00000677155,ENST00000485344,ENST00000678898,ENST00000414580,ENST00000678289,ENST00000678158,ENST00000676929,ENST00000676491,ENST00000677407,ENST00000448880,ENST00000677490,ENST00000677263,ENST00000404554,ENST00000444548,ENST00000679183,ENST00000620965,ENST00000679308,ENST00000677567,ENST00000677114,MSTRG.20164.35,MSTRG.20164.1,ENST00000676782,ENST00000307300,MSTRG.20164.40,MSTRG.20164.6,ENST00000443626,ENST00000419408	ENST00000678188	10	-	2	240011616	240007371	240007370	240007316	239990182	239990074	239990073	239961187	7	9	3	4245	54	108	28886	81	16565.5	250	1
ENSG00000148498:-:10:34470084:34517159	ENSG00000148498	ENST00000696673,ENST00000374789,ENST00000374788,ENST00000545693,ENST00000346874,ENST00000350537,ENST00000374790,ENST00000545260,ENST00000374794,MSTRG.3777.10,ENST00000340077,MSTRG.3777.16,ENST00000374773,ENST00000374776,MSTRG.3777.17,MSTRG.3777.18,MSTRG.3777.20	ENST00000696673	24	-	10	34678860	34517160	34517159	34516979	34470263	34470085	34470084	34450449	2	3	2	161700	180	178	19635	179	90667.5	360	1
ENSG00000143207:-:1:175986942:176149074	ENSG00000143207	ENST00000649803,ENST00000367669,MSTRG.2642.7,MSTRG.2642.4,ENST00000367667,ENST00000308769,ENST00000367666	ENST00000649803	21	-	1	176162868	176149075	176149074	176149006	175987103	175986943	175986942	175947240	7	19	13	13793	68	160	39702	114	26747.5	1371	1
ENSG00000166813:-:15:89645012:89649941	ENSG00000166813	ENST00000696512,ENST00000394412,MSTRG.11504.5,MSTRG.11504.1,MSTRG.11504.7,MSTRG.11504.9,MSTRG.11504.10,MSTRG.11504.11	ENST00000696512	19	-	15	89652602	89649942	89649941	89649741	89645165	89645013	89645012	89642406	3	10	8	2660	200	152	2606	176	2633	1863	1
ENSG00000197905:+:12:2994737:2994992	ENSG00000197905	MSTRG.6628.3,MSTRG.6628.4,ENST00000359864,MSTRG.6628.2,ENST00000358409,ENST00000543035,ENST00000540314,ENST00000443986,ENST00000536826	MSTRG.6628.3	13	+	12	2960354	2994737	2994738	2994992	2994738	2994992	2994993	3011003	3	3	1	34383	254	254	16010	254	25196.5	255	1
ENSG00000150760:+:10:127008731:127061776	ENSG00000150760	MSTRG.4806.1,ENST00000623213,ENST00000280333	MSTRG.4806.1	38	+	10	127000308	127008731	127008732	127008804	127061668	127061776	127061777	127106230	11	23	13	8423	72	108	44453	90	26438	1353	1
ENSG00000089154:-:12:120134290:120153909	ENSG00000089154	ENST00000300648	ENST00000300648	58	-	12	120154969	120153910	120153909	120153744	120134405	120134291	120134290	120132023	32	53	22	1059	165	114	2267	139.5	1663	3616	1
ENSG00000136381:+:15:78465250:78483434	ENSG00000136381	ENST00000258886,ENST00000558570,MSTRG.11310.6	ENST00000258886	22	+	15	78463088	78465250	78465251	78465388	78483318	78483434	78483435	78484760	4	11	8	2162	137	116	1325	126.5	1743.5	1141	1
ENSG00000048828:+:9:93471140:93476338	ENSG00000048828	ENST00000277165,MSTRG.33016.2,MSTRG.33016.4,MSTRG.33016.6,MSTRG.33016.5,MSTRG.33016.8,MSTRG.33016.9,MSTRG.33016.10,ENST00000375389,ENST00000446420	ENST00000277165	18	+	9	93452390	93471140	93471141	93471387	93476256	93476338	93476339	93497470	2	3	2	18750	246	82	21131	164	19940.5	330	1
ENSG00000168246:-:5:172234121:172234358	ENSG00000168246	ENST00000393792,MSTRG.27301.3,MSTRG.27301.1,MSTRG.27301.4	ENST00000393792	3	-	5	172283595	172234359	172234358	172234122	172234358	172234122	172234121	172212228	2	2	1	49236	236	236	21893	236	35564.5	237	1
ENSG00000116539:-:1:155370503:155395553	ENSG00000116539	ENST00000679097,ENST00000678117,ENST00000677213,ENST00000676814,ENST00000392403,ENST00000368346,MSTRG.2319.1,ENST00000679133,ENST00000679333,ENST00000677042,ENST00000678699,ENST00000677825,MSTRG.2319.14	ENST00000679097	29	-	1	155415743	155395554	155395553	155395459	155370650	155370504	155370503	155360410	8	13	6	20189	94	146	10093	120	15141	678	1
ENSG00000106066:-:7:29064060:29072423	ENSG00000106066	ENST00000409850,ENST00000265394,ENST00000396276	ENST00000409850	17	-	7	29086483	29072424	29072423	29072301	29064234	29064061	29064060	29030760	12	15	4	14059	122	173	33300	147.5	23679.5	528	1
ENSG00000070961:-:12:89630504:89642355	ENSG00000070961	MSTRG.7745.3,ENST00000428670,ENST00000359142,ENST00000261173,MSTRG.7745.6,MSTRG.7745.7	MSTRG.7745.3	21	-	12	89655678	89642356	89642355	89642158	89630645	89630505	89630504	89627717	3	6	4	13322	197	140	2787	168.5	8054.5	720	1
ENSG00000085491:-:1:108148278:108161293	ENSG00000085491	ENST00000565488,ENST00000648874,ENST00000264128,ENST00000370041,MSTRG.1719.5	ENST00000565488	10	-	1	108181940	108161294	108161293	108161182	108148386	108148279	108148278	108143711	4	7	4	20646	111	107	4567	109	12606.5	532	1
ENSG00000148484:-:10:16752538:16764510	ENSG00000148484	ENST00000377921,ENST00000345264,ENST00000602389,MSTRG.3609.4,ENST00000464074,MSTRG.3609.5,ENST00000377911	ENST00000377921	8	-	10	16782033	16764511	16764510	16764390	16752653	16752539	16752538	16695156	3	6	4	17522	120	114	57382	117	37452	438	1
ENSG00000156374:-:10:103314185:103326632	ENSG00000156374	ENST00000369847,ENST00000647574,ENST00000337211,ENST00000490296,MSTRG.4563.6,MSTRG.4563.5	ENST00000369847	10	-	10	103333924	103326633	103326632	103326534	103314272	103314186	103314185	103303962	8	9	2	7291	98	86	10223	92	8757	186	1
ENSG00000288637:+:4:153322647:153394336	ENSG00000288637	ENST00000674680,ENST00000675079,ENST00000675838,ENST00000674967,MSTRG.25634.65,ENST00000675518	ENST00000674680	19	+	4	153316000	153322647	153322648	153322816	153394262	153394336	153394337	153397218	9	17	9	6647	168	74	2881	121	4764	2351	1
ENSG00000243156:-:22:17864898:17906886	ENSG00000243156	ENST00000672019,ENST00000441493,MSTRG.21576.3,MSTRG.21576.5,MSTRG.21576.6,MSTRG.21576.7,MSTRG.21576.8,MSTRG.21576.10,MSTRG.21576.9,MSTRG.21576.12,ENST00000495076,ENST00000414725,ENST00000400561,ENST00000383094	ENST00000672019	33	-	22	18024280	17906887	17906886	17906549	17864986	17864899	17864898	17842018	2	19	18	117393	337	87	22880	212	70136.5	2679	1
ENSG00000131437:-:5:132699170:132711057	ENSG00000131437	ENST00000403231,ENST00000378746,ENST00000618515,ENST00000378735	ENST00000403231	19	-	5	132715756	132711058	132711057	132710959	132699295	132699171	132699170	132696683	9	18	10	4698	98	124	2487	111	3592.5	1003	1
ENSG00000060749:+:11:32927156:32935435	ENSG00000060749	ENST00000650167,ENST00000399302,MSTRG.5334.3,MSTRG.5334.4,MSTRG.5334.5,ENST00000527788	ENST00000650167	13	+	11	32893335	32927156	32927157	32927269	32931743	32935435	32935436	32953856	2	4	3	33821	112	3692	18420	1902	26120.5	3968	1
ENSG00000156639:+:6:38061592:38116739	ENSG00000156639	ENST00000287218,ENST00000373391,MSTRG.28232.3,ENST00000474522,ENST00000373389	ENST00000287218	6	+	6	37930000	38061592	38061593	38061775	38116572	38116739	38116740	38152234	3	5	3	131592	182	167	35494	174.5	83543	417	1
ENSG00000171490:-:16:11841694:11847806	ENSG00000171490	ENST00000571133,ENST00000396503,ENST00000355674,ENST00000573251	ENST00000571133	9	-	16	11850278	11847807	11847806	11847668	11841820	11841695	11841694	11839986	3	7	5	2471	138	125	1708	131.5	2089.5	610	1
ENSG00000112290:-:6:110127468:110127629	ENSG00000112290	ENST00000392588,ENST00000359451,ENST00000392589,ENST00000392586,ENST00000392587,MSTRG.28774.13,MSTRG.28774.7,MSTRG.28774.8,MSTRG.28774.10,MSTRG.28774.12,MSTRG.28774.15,MSTRG.28774.9,MSTRG.28774.16,MSTRG.28774.6,MSTRG.28774.14,MSTRG.28774.11,ENST00000265601,ENST00000444391,ENST00000447287,ENST00000368938,ENST00000419252	ENST00000392588	10	-	6	110178597	110127630	110127629	110127469	110127629	110127469	110127468	110113461	3	3	1	50967	160	160	14007	160	32487	161	1
ENSG00000242259_ENSG00000070010:-:22:19447377:19458139	ENSG00000242259_ENSG00000070010	MSTRG.21629.23,MSTRG.21629.24	MSTRG.21629.23	15	-	22	19465201	19458140	19458139	19458071	19447545	19447378	19447377	19444391	7	13	7	7061	68	167	2986	117.5	5023.5	3602	1
ENSG00000159335:+:12:6769602:6769999	ENSG00000159335	ENST00000389462,ENST00000309083,MSTRG.6713.3,MSTRG.6713.5,MSTRG.6713.6,MSTRG.6713.7,ENST00000538057,ENST00000540667	ENST00000389462	5	+	12	6766751	6769602	6769603	6769674	6769844	6769999	6770000	6770156	2	3	2	2851	71	155	156	113	1503.5	228	1
ENSG00000163468:-:1:156333546:156335888	ENSG00000163468	ENST00000368262,MSTRG.2358.4,ENST00000295688,MSTRG.2358.6,ENST00000472765,MSTRG.2358.7,ENST00000368259,ENST00000368256,ENST00000496684,ENST00000413555,ENST00000415548,ENST00000478640,ENST00000446905	ENST00000368262	15	-	1	156337078	156335889	156335888	156335827	156333643	156333547	156333546	156325090	3	6	4	1189	61	96	8456	78.5	4822.5	273	1
ENSG00000174915:+:11:460186:460288	ENSG00000174915	ENST00000308020,MSTRG.4894.3,MSTRG.4894.7,MSTRG.4894.2,MSTRG.4894.11,ENST00000531520,ENST00000525727,ENST00000528617,ENST00000527479,ENST00000532614	ENST00000308020	12	+	11	450638	460186	460187	460288	460187	460288	460289	473894	2	2	1	9548	101	101	13605	101	11576.5	102	1
ENSG00000169855:-:3:78639743:78647655	ENSG00000169855	ENST00000436010,MSTRG.23281.4,ENST00000464233,MSTRG.23281.5,ENST00000498428	ENST00000436010	29	-	3	78651731	78647656	78647655	78647629	78639898	78639744	78639743	78636109	18	20	3	4075	26	154	3634	90	3854.5	225	1
ENSG00000284946:-:15:90984017:90999975	ENSG00000284946	ENST00000643536,MSTRG.11570.1,ENST00000647331	ENST00000643536	35	-	15	91000489	90999976	90999975	90999900	90984140	90984018	90984017	90981982	21	24	4	513	75	122	2035	98.5	1274	449	1
ENSG00000285043:+:16:30069305:30070029	ENSG00000285043	ENST00000566897,ENST00000395248,MSTRG.12431.1,ENST00000338110,MSTRG.12431.6	ENST00000566897	12	+	16	30068979	30069305	30069306	30069389	30069830	30070029	30070030	30070116	9	11	3	326	83	199	86	141	206	459	1
ENSG00000174564:+:3:136980465:136995556	ENSG00000174564	ENST00000329582	ENST00000329582	7	+	3	136958202	136980465	136980466	136980592	136995414	136995556	136995557	137010112	2	6	5	22263	126	142	14555	134	18409	737	1
ENSG00000068885:-:3:160356012:160381718	ENSG00000068885	ENST00000483465,ENST00000487943,ENST00000496589,MSTRG.24013.3	ENST00000483465	19	-	3	160383436	160381719	160381718	160381503	160356150	160356013	160356012	160319940	2	7	6	1717	215	137	36072	176	18894.5	734	1
ENSG00000179818:-:2:70051202:70051305	ENSG00000179818	ENST00000682294,ENST00000684630,ENST00000630975,MSTRG.18633.158,MSTRG.18633.166,ENST00000419542,ENST00000655086,ENST00000653606,ENST00000653596,ENST00000654336,ENST00000660903,ENST00000668711,ENST00000669583,ENST00000659941,ENST00000661703,ENST00000654601,ENST00000655058,ENST00000670785,ENST00000664754,ENST00000625955,ENST00000654716,ENST00000661989,ENST00000665838,ENST00000670313,ENST00000654878,ENST00000665172,ENST00000663209,ENST00000662103,ENST00000654958,ENST00000687961,ENST00000656308,ENST00000630520,ENST00000653064,ENST00000664773,ENST00000655140,ENST00000439670,ENST00000689275,ENST00000667952,ENST00000664419,ENST00000663375,ENST00000668191,ENST00000626509,ENST00000416395,ENST00000670974,ENST00000664014,ENST00000449178,ENST00000425601,ENST00000435880,ENST00000626558,ENST00000659531,ENST00000664049,ENST00000659305,ENST00000656326,ENST00000458698,ENST00000670786,ENST00000667307,ENST00000686055,ENST00000657575,ENST00000653289,MSTRG.18633.9,ENST00000658454,ENST00000653811,ENST00000661863,ENST00000670036,ENST00000612202,MSTRG.18633.190,ENST00000664958,ENST00000688473,ENST00000655096,ENST00000655481,MSTRG.18633.254,ENST00000657164,ENST00000657291,ENST00000629339,ENST00000670845,ENST00000667874,MSTRG.18633.255,ENST00000437019,ENST00000662224,ENST00000685037,ENST00000659586,ENST00000692564,ENST00000420309,ENST00000691287,ENST00000609543,MSTRG.18633.257,ENST00000669812,ENST00000686517,ENST00000664533,ENST00000653114,ENST00000689138,ENST00000685127,MSTRG.18633.28,ENST00000665708,ENST00000660404,ENST00000688056,ENST00000688045,ENST00000457770,ENST00000630214,ENST00000665264,ENST00000690200,ENST00000692333,ENST00000687866,ENST00000625788,ENST00000629836,ENST00000685079,ENST00000684907,ENST00000691987,ENST00000629533,MSTRG.18633.256,ENST00000685803,ENST00000627327,ENST00000664483,ENST00000684857,ENST00000601431,ENST00000627918,ENST00000596028,ENST00000663033,ENST00000594376,ENST00000413436,ENST00000452431,ENST00000442040,ENST00000630283,ENST00000429599,ENST00000625625,ENST00000413791,ENST00000458686,ENST00000626515,ENST00000628305,ENST00000688311,ENST00000628308,ENST00000423402,ENST00000434781,ENST00000630746,ENST00000625518,ENST00000457076,ENST00000690464,ENST00000599427,ENST00000626898,ENST00000630377,ENST00000425333,ENST00000627050,ENST00000627398,ENST00000629605,ENST00000630759,ENST00000413069,ENST00000366234,ENST00000626574,ENST00000456161,ENST00000629188,ENST00000415222,ENST00000628883,ENST00000628659,ENST00000629603,ENST00000418308,ENST00000627074,ENST00000630522,ENST00000625447,ENST00000625215,ENST00000631107,ENST00000631110,ENST00000625783,ENST00000627487,ENST00000628551,ENST00000444320,ENST00000628837,ENST00000626609,ENST00000629407,ENST00000629212,ENST00000625864,ENST00000629825,ENST00000628374,ENST00000629485,ENST00000629084,ENST00000597318,ENST00000628263,ENST00000626495,ENST00000626842,ENST00000628667,ENST00000627189,ENST00000603347,ENST00000629374,ENST00000626370,ENST00000629415,ENST00000629184,ENST00000626162,ENST00000625227,ENST00000601396,ENST00000421255,ENST00000421843,ENST00000604346,ENST00000599673,ENST00000628322,ENST00000626632,ENST00000629506,ENST00000625490,ENST00000630685,ENST00000627325,ENST00000690924,ENST00000630985,ENST00000419963,ENST00000416506,ENST00000630280,ENST00000411429,ENST00000418564,ENST00000601395,ENST00000628147,ENST00000614826,ENST00000627155,ENST00000432604,ENST00000629178,ENST00000415742,ENST00000626440,ENST00000444410,MSTRG.18633.258	ENST00000682294	8	-	2	70085546	70051306	70051305	70051203	70051305	70051203	70051202	70018132	3	3	1	34240	102	102	33070	102	33655	103	1
ENSG00000188352:+:9:20819795:20823115	ENSG00000188352	ENST00000380249,MSTRG.32470.1,MSTRG.32470.4,ENST00000338382,MSTRG.32470.5	ENST00000380249	46	+	9	20789609	20819795	20819796	20819900	20822989	20823115	20823116	20862577	14	17	4	30186	104	126	39461	115	34823.5	465	1
ENSG00000124788:-:6:16657775:16657901	ENSG00000124788	ENST00000244769,ENST00000436367,ENST00000646259,ENST00000473388,ENST00000675689,MSTRG.27684.7,MSTRG.27684.9,MSTRG.27684.8,ENST00000483591,ENST00000495178,MSTRG.27684.4	ENST00000244769	9	-	6	16753232	16657902	16657901	16657776	16657901	16657776	16657775	16585908	4	4	1	95330	125	125	71867	125	83598.5	126	1
ENSG00000113013:-:5:138568924:138571141	ENSG00000113013	ENST00000649692,ENST00000677693,ENST00000677988,ENST00000297185,ENST00000677553,ENST00000678300,ENST00000678794,ENST00000677066,ENST00000677527,ENST00000649578,ENST00000677425,ENST00000677064,ENST00000678384,ENST00000678051,ENST00000524109,ENST00000678551,ENST00000508003,MSTRG.27005.17,ENST00000507115,ENST00000507097,ENST00000504902	ENST00000649692	12	-	5	138573762	138571142	138571141	138570960	138569049	138568925	138568924	138567723	4	5	2	2620	181	124	1201	152.5	1910.5	307	1
ENSG00000075218:+:22:46326435:46326654	ENSG00000075218	MSTRG.22339.2,ENST00000454366,MSTRG.22339.4,MSTRG.22339.7,ENST00000479645	MSTRG.22339.2	11	+	22	46323263	46326435	46326436	46326654	46326436	46326654	46326655	46328687	8	8	1	3172	218	218	2032	218	2602	219	1
ENSG00000075240:+:22:46626750:46626961	ENSG00000075240	MSTRG.22342.2,ENST00000406902,ENST00000447351	MSTRG.22342.2	19	+	22	46577291	46626750	46626751	46626961	46626751	46626961	46626962	46637839	2	2	1	49459	210	210	10877	210	30168	211	1
ENSG00000171316:+:8:60741258:60781430	ENSG00000171316	ENST00000423902,MSTRG.31555.2,MSTRG.31555.6,ENST00000695853,MSTRG.31555.7,MSTRG.31555.8,ENST00000695849,ENST00000695848	ENST00000423902	38	+	8	60679083	60741258	60741259	60743097	60781000	60781430	60781431	60794985	2	3	2	62175	1838	430	13554	1134	37864.5	2270	1
ENSG00000189159:-:17:75148528:75174823	ENSG00000189159	MSTRG.15055.9,MSTRG.15055.15	MSTRG.15055.9	8	-	17	75181056	75174824	75174823	75174752	75148671	75148529	75148528	75147654	3	5	3	6232	71	142	874	106.5	3553	383	1
ENSG00000124299:-:19:33462994:33464062	ENSG00000124299	MSTRG.16937.1,MSTRG.16937.7,ENST00000588328,ENST00000244137,ENST00000651901,ENST00000436370,MSTRG.16937.11,ENST00000590731,ENST00000609145,ENST00000588719	MSTRG.16937.1	15	-	19	33478045	33464063	33464062	33463987	33463041	33462995	33462994	33413644	8	9	2	13982	75	46	49350	60.5	31666	123	1
ENSG00000120694:-:13:31145562:31145768	ENSG00000120694	ENST00000320027,ENST00000445273,ENST00000626866,MSTRG.8556.3,ENST00000630972,ENST00000602786,ENST00000380405,MSTRG.8556.9,MSTRG.8556.8	ENST00000320027	18	-	13	31147958	31145769	31145768	31145563	31145768	31145563	31145562	31143924	11	11	1	2189	205	205	1638	205	1913.5	206	1
ENSG00000160194:+:21:42896926:42897047	ENSG00000160194	ENST00000354250,MSTRG.21397.8,MSTRG.21397.10,MSTRG.21397.13,MSTRG.21397.15,MSTRG.21397.7,ENST00000340344,MSTRG.21397.9,MSTRG.21397.17,MSTRG.21397.5,MSTRG.21397.11,MSTRG.21397.12,MSTRG.21397.14,MSTRG.21397.18,ENST00000460259,MSTRG.21397.6	ENST00000354250	4	+	21	42893382	42896926	42896927	42897047	42896927	42897047	42897048	42903181	2	2	1	3544	120	120	6133	120	4838.5	121	1
ENSG00000109606:-:4:24570653:24576678	ENSG00000109606	ENST00000336812,MSTRG.24700.6,MSTRG.24700.2,MSTRG.24700.11,MSTRG.24700.5,MSTRG.24700.9,MSTRG.24700.12,MSTRG.24700.10	ENST00000336812	14	-	4	24584322	24576679	24576678	24576243	24570847	24570654	24570653	24556411	2	3	2	7643	435	193	14242	314	10942.5	630	1
ENSG00000033867:-:3:27383152:27452498	ENSG00000033867	ENST00000454389,ENST00000425128,ENST00000295736,ENST00000428386,ENST00000440156,MSTRG.22628.6,ENST00000445684,ENST00000446700,ENST00000438530,ENST00000455077,ENST00000437179,MSTRG.22628.14,ENST00000457377,ENST00000437266	ENST00000454389	26	-	3	27484066	27452499	27452498	27452417	27383250	27383153	27383152	27379357	2	24	23	31567	81	97	3795	89	17681	3530	1
ENSG00000044115:+:5:138827514:138887642	ENSG00000044115	ENST00000627109,ENST00000302763,ENST00000518825,MSTRG.27006.5,MSTRG.27006.11,ENST00000521724,ENST00000523275	ENST00000627109	19	+	5	138824800	138827514	138827515	138827718	138887490	138887642	138887643	138904348	7	9	3	2714	203	152	16705	177.5	9709.5	438	1
ENSG00000141564:+:17:80625690:80925480	ENSG00000141564	ENST00000306801,MSTRG.15244.6,MSTRG.15244.1,MSTRG.15244.2,ENST00000544334	ENST00000306801	34	+	17	80545792	80625690	80625691	80625793	80925370	80925480	80925481	80940495	2	24	23	79898	102	110	15014	106	47456	2757	1
ENSG00000103202:+:16:399378:399739	ENSG00000103202	MSTRG.11721.3,MSTRG.11721.18,MSTRG.11721.15,MSTRG.11721.22,ENST00000460297,MSTRG.11721.2,MSTRG.11721.4,ENST00000450036,MSTRG.11721.9,MSTRG.11721.11,MSTRG.11721.23,ENST00000397722,ENST00000620944,ENST00000448828,ENST00000444498,ENST00000433358,ENST00000382940,ENST00000219479	MSTRG.11721.3	13	+	16	399124	399378	399379	399480	399627	399739	399740	404955	4	5	2	254	101	112	5215	106.5	2734.5	215	1
ENSG00000149311:+:11:108333885:108347365	ENSG00000149311	ENST00000683174,MSTRG.6277.10,ENST00000452508,ENST00000675843,ENST00000278616,MSTRG.6277.12,MSTRG.6277.5,ENST00000675595,ENST00000527805,ENST00000684152,ENST00000683524,ENST00000682286,ENST00000682302,ENST00000524792,ENST00000638786,ENST00000684180	ENST00000683174	63	+	11	108332901	108333885	108333886	108333968	108347279	108347365	108347366	108353765	54	59	6	984	82	86	6399	84	3691.5	744	1
ENSG00000131773:+:8:135542653:135582073	ENSG00000131773	MSTRG.32133.1,ENST00000355849,ENST00000524199	MSTRG.32133.1	11	+	8	135521356	135542653	135542654	135542770	135581878	135582073	135582074	135606954	3	6	4	21297	116	195	24880	155.5	23088.5	600	1
ENSG00000179526:-:8:144099928:144103225	ENSG00000179526	ENST00000359551,MSTRG.32246.7,MSTRG.32246.1,MSTRG.32246.16,MSTRG.32246.3,MSTRG.32246.12,MSTRG.32246.15,MSTRG.32246.4,MSTRG.32246.2,ENST00000398712,MSTRG.32246.8,MSTRG.32246.11,MSTRG.32246.6,MSTRG.32246.9,MSTRG.32246.17,MSTRG.32246.10,ENST00000533948,ENST00000534242	ENST00000359551	8	-	8	144103552	144103226	144103225	144103051	144100069	144099929	144099928	144099845	2	3	2	326	174	140	83	157	204.5	316	1
ENSG00000160087:-:1:1263345:1267992	ENSG00000160087	ENST00000450390,ENST00000347370,ENST00000349431,MSTRG.77.4,ENST00000464036,ENST00000360466,MSTRG.77.6,ENST00000435198,MSTRG.77.10,ENST00000400930,ENST00000473215,ENST00000466752,ENST00000503294,ENST00000477894,ENST00000422076,ENST00000502382	ENST00000450390	8	-	1	1273665	1267993	1267992	1267862	1263386	1263346	1263345	1257311	2	4	3	5672	130	40	6034	85	5853	365	1
ENSG00000136527:-:3:185923795:185931852	ENSG00000136527	ENST00000487615,ENST00000456380	ENST00000487615	10	-	3	185934428	185931853	185931852	185931577	185923984	185923796	185923795	185922127	2	5	4	2575	275	188	1668	231.5	2121.5	762	1
ENSG00000159692:-:4:1228198:1238337	ENSG00000159692	ENST00000382952,MSTRG.24434.3,ENST00000290921,MSTRG.24434.6,MSTRG.24434.5,MSTRG.24434.9,MSTRG.24434.15,MSTRG.24434.19,MSTRG.24434.14,MSTRG.24434.8,MSTRG.24434.16,MSTRG.24434.13,MSTRG.24434.17,MSTRG.24434.11,MSTRG.24434.26,ENST00000514210,ENST00000515399,ENST00000513420,ENST00000506180,MSTRG.24434.24	ENST00000382952	10	-	4	1241324	1238338	1238337	1238183	1228343	1228199	1228198	1225567	3	4	2	2986	154	144	2631	149	2808.5	300	1
ENSG00000075151:-:1:21050865:21111383	ENSG00000075151	MSTRG.488.20,MSTRG.488.27,ENST00000356916,ENST00000682284	MSTRG.488.20	36	-	1	21176721	21111384	21111383	21111323	21050994	21050866	21050865	21002809	2	4	3	65337	60	128	48056	94	56696.5	266	1
ENSG00000205323:-:12:55789074:55800900	ENSG00000205323	MSTRG.7426.1,ENST00000546604,MSTRG.7426.9,MSTRG.7426.6,ENST00000336133,ENST00000552080	MSTRG.7426.1	11	-	12	55803628	55800901	55800900	55800854	55789143	55789075	55789074	55760641	3	9	7	2727	46	68	28433	57	15580	365	1
ENSG00000153560:-:3:33411580:33425741	ENSG00000153560	ENST00000283629,ENST00000447368,MSTRG.22687.1,MSTRG.22687.4,MSTRG.22687.8,MSTRG.22687.6,MSTRG.22687.14,MSTRG.22687.9,ENST00000283628,MSTRG.22687.10,MSTRG.22687.12,MSTRG.22687.16	ENST00000283629	16	-	3	33439735	33425742	33425741	33425590	33411687	33411581	33411580	33409602	2	5	4	13993	151	106	1978	128.5	7985.5	442	1
ENSG00000197157:+:7:127887901:127929329	ENSG00000197157	ENST00000354725,MSTRG.30622.1,MSTRG.30622.5,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.6,MSTRG.30622.2,MSTRG.30622.4,ENST00000465900	ENST00000354725	24	+	7	127844425	127887901	127887902	127888012	127929188	127929329	127929330	127990946	13	15	3	43476	110	141	61616	125.5	52546	326	1
ENSG00000234545:-:7:92575770:92579395	ENSG00000234545	ENST00000438306,ENST00000445716,ENST00000481407,ENST00000415397,MSTRG.30217.5,ENST00000427372,MSTRG.30217.6	ENST00000438306	12	-	7	92581505	92579396	92579395	92579317	92575821	92575771	92575770	92569916	4	9	6	2109	78	50	5854	64	3981.5	394	1
ENSG00000136938:+:9:97998555:97998678	ENSG00000136938	MSTRG.33115.2,MSTRG.33115.4,MSTRG.33115.6,MSTRG.33115.5,MSTRG.33115.9,MSTRG.33115.1,ENST00000339399,MSTRG.33115.15,MSTRG.33115.14,ENST00000473205	MSTRG.33115.2	7	+	9	97994781	97998555	97998556	97998678	97998556	97998678	97998679	98004963	3	3	1	3774	122	122	6284	122	5029	123	1
ENSG00000181090_ENSG00000197070:+:9:137754170:137800984	ENSG00000181090_ENSG00000197070	MSTRG.33733.4,MSTRG.33733.1	MSTRG.33733.4	31	+	9	137744091	137754170	137754171	137754291	137800880	137800984	137800985	137811460	12	22	11	10079	120	104	10475	112	10277	1464	1
ENSG00000135945:-:2:99462495:99464985	ENSG00000135945	MSTRG.18938.7,ENST00000258428,ENST00000393445,ENST00000413697,MSTRG.18938.4,MSTRG.18938.10	MSTRG.18938.7	21	-	2	99489816	99464986	99464985	99464922	99462622	99462496	99462495	99449505	2	3	2	24830	63	126	12990	94.5	18910	191	1
ENSG00000137815:+:15:41438320:41470392	ENSG00000137815	ENST00000389629,MSTRG.10601.4	ENST00000389629	18	+	15	41417314	41438320	41438321	41438431	41470257	41470392	41470393	41471171	2	7	6	21006	110	135	778	122.5	10892	827	1
ENSG00000105281:-:19:46776974:46784559	ENSG00000105281	ENST00000542575,MSTRG.17384.5,ENST00000412532,MSTRG.17384.6,MSTRG.17384.7	ENST00000542575	8	-	19	46787399	46784560	46784559	46784517	46777109	46776975	46776974	46775748	2	7	6	2839	42	134	1226	88	2032.5	822	1
ENSG00000079156:+:2:178284926:178285121	ENSG00000079156	ENST00000190611,ENST00000359685,MSTRG.19582.1,ENST00000392505,MSTRG.19582.7,MSTRG.19582.4,ENST00000409045,MSTRG.19582.10,MSTRG.19582.11,ENST00000409631,ENST00000357080,ENST00000477646	ENST00000190611	25	+	2	178194675	178284926	178284927	178285121	178284927	178285121	178285122	178306029	2	2	1	90251	194	194	20907	194	55579	195	1
ENSG00000138777:-:4:105424195:105456745	ENSG00000138777	ENST00000341695,ENST00000348706,ENST00000509426,MSTRG.25266.3,ENST00000509031,MSTRG.25266.10,MSTRG.25266.19,MSTRG.25266.18,ENST00000432483,ENST00000502833,ENST00000457404,ENST00000508518,ENST00000510015	ENST00000341695	12	-	4	105473893	105456746	105456745	105456681	105424322	105424196	105424195	105399165	2	7	6	17147	64	126	25030	95	21088.5	498	1
ENSG00000127663:+:19:5143966:5144902	ENSG00000127663	ENST00000611640,ENST00000159111,MSTRG.16238.4,ENST00000536461,ENST00000589104	ENST00000611640	24	+	19	5138071	5143966	5143967	5144152	5144783	5144902	5144903	5150357	20	22	3	5895	185	119	5454	152	5674.5	471	1
ENSG00000086200:+:5:62437273:62515501	ENSG00000086200	ENST00000409296,ENST00000325324,MSTRG.26266.26,ENST00000424533	ENST00000409296	30	+	5	62419128	62437273	62437274	62437417	62515388	62515501	62515502	62526141	2	20	19	18145	143	113	10639	128	14392	1902	1
ENSG00000158417:+:2:99360235:99364421	ENSG00000158417	MSTRG.18937.2,MSTRG.18937.1,ENST00000617677,MSTRG.18937.4,ENST00000289371,MSTRG.18937.6,MSTRG.18937.5,MSTRG.18937.9,MSTRG.18937.8	MSTRG.18937.2	24	+	2	99337590	99360235	99360236	99360361	99364271	99364421	99364422	99368492	2	6	5	22645	125	150	4070	137.5	13357.5	1253	1
ENSG00000126870:+:7:158869854:158876691	ENSG00000126870	MSTRG.31006.2,ENST00000407559,MSTRG.31006.6,MSTRG.31006.4,MSTRG.31006.1,MSTRG.31006.5	MSTRG.31006.2	27	+	7	158856751	158869854	158869855	158869908	158876609	158876691	158876692	158879683	2	4	3	13103	53	82	2991	67.5	8047	558	1
ENSG00000173889:-:3:170122590:170129552	ENSG00000173889	ENST00000495893,ENST00000494943,MSTRG.24051.3,MSTRG.24051.2,MSTRG.24051.1,ENST00000467570	ENST00000495893	15	-	3	170136418	170129553	170129552	170128684	170122744	170122591	170122590	170117477	8	9	2	6865	868	153	5113	510.5	5989	1023	1
ENSG00000263001:+:7:74705163:74718941	ENSG00000263001	MSTRG.30058.5,ENST00000650807,MSTRG.30058.6,ENST00000573035,ENST00000614986	MSTRG.30058.5	34	+	7	74700635	74705163	74705164	74705218	74718879	74718941	74718942	74728785	8	12	5	4528	54	62	9843	58	7185.5	297	1
ENSG00000102189:-:12:92809016:92813093	ENSG00000102189	ENST00000322349,ENST00000418984	ENST00000322349	29	-	12	92816199	92813094	92813093	92812980	92809156	92809017	92809016	92802735	16	18	3	3105	113	139	6281	126	4693	410	1
ENSG00000135365:-:11:46076753:46092223	ENSG00000135365	ENST00000676320,ENST00000693049,MSTRG.5442.5,ENST00000530587,MSTRG.5442.3,ENST00000323180,ENST00000690620,ENST00000689695,MSTRG.5442.6,ENST00000418153,MSTRG.5442.12,MSTRG.5442.16,MSTRG.5442.19,ENST00000687985,ENST00000688222,ENST00000525676,MSTRG.5442.20,MSTRG.5442.18,MSTRG.5442.15,MSTRG.5442.21,ENST00000685188,ENST00000691784,ENST00000693122,ENST00000532883,ENST00000524497,ENST00000529782,ENST00000531959,ENST00000533757	ENST00000676320	19	-	11	46120934	46092224	46092223	46092183	46076819	46076754	46076753	45979967	2	6	5	28710	40	65	96786	52.5	62748	389	1
ENSG00000157869:-:4:13376544:13460828	ENSG00000157869	ENST00000288723,ENST00000338176,ENST00000330852,MSTRG.24631.2,ENST00000511649	ENST00000288723	8	-	4	13474317	13460829	13460828	13460699	13376622	13376545	13376544	13369966	4	6	3	13488	129	77	6578	103	10033	312	1
ENSG00000101057:+:20:43673805:43687072	ENSG00000101057	MSTRG.20728.21,MSTRG.20728.9,MSTRG.20728.26,MSTRG.20728.31,MSTRG.20728.16,ENST00000396863,ENST00000217026,MSTRG.20728.13,MSTRG.20728.36,MSTRG.20728.10,MSTRG.20728.28,MSTRG.20728.2,MSTRG.20728.33,MSTRG.20728.5,MSTRG.20728.1,MSTRG.20728.7,MSTRG.20728.38,MSTRG.20728.11,MSTRG.20728.27,MSTRG.20728.37,MSTRG.20728.14,MSTRG.20728.34,MSTRG.20728.8,MSTRG.20728.29,MSTRG.20728.39,MSTRG.20728.15,MSTRG.20728.3	MSTRG.20728.21	15	+	20	43671434	43673805	43673806	43673899	43686852	43687072	43687073	43692156	3	6	4	2371	93	220	5083	156.5	3727	509	1
ENSG00000164638:+:7:5287805:5287985	ENSG00000164638	ENST00000396872,ENST00000297195,ENST00000406453,MSTRG.29434.4,MSTRG.29434.6,MSTRG.29434.7,MSTRG.29434.8,ENST00000434816,ENST00000444741	ENST00000396872	11	+	7	5283083	5287808	5287809	5287985	5287809	5287985	5287986	5290731	2	2	1	4725	176	176	2745	176	3735	177	1
ENSG00000175581:+:11:73843246:73844976	ENSG00000175581	ENST00000497094	ENST00000497094	6	+	11	73825797	73843246	73843247	73843367	73844807	73844976			5	6	2	17449	120	169		144.5	17449	291	1
ENSG00000095380:+:9:98060781:98060997	ENSG00000095380	MSTRG.33120.3,ENST00000210444,MSTRG.33120.5,MSTRG.33120.2,MSTRG.33120.6,ENST00000495319	MSTRG.33120.3	6	+	9	98045840	98060781	98060782	98060997	98060782	98060997	98060998	98076917	2	2	1	14941	215	215	15919	215	15430	216	1
ENSG00000272391:-:7:75439143:75441115	ENSG00000272391	MSTRG.30089.1,ENST00000615331,MSTRG.30089.2,ENST00000607367,MSTRG.30089.7,MSTRG.30089.8,MSTRG.30089.12	MSTRG.30089.1	17	-	7	75441431	75441116	75441115	75440954	75439224	75439144	75439143	75437687	6	7	2	315	161	80	1456	120.5	885.5	243	1
ENSG00000166598:+:12:103947310:103947430	ENSG00000166598	ENST00000681704,ENST00000680478,ENST00000680653,ENST00000679779,ENST00000681910,ENST00000681245,ENST00000680989,ENST00000680281,ENST00000680391,ENST00000548462,ENST00000681174,ENST00000550595,ENST00000681949,ENST00000680899,ENST00000680370,ENST00000681172,ENST00000681941,ENST00000299767,ENST00000679538,ENST00000679861,ENST00000680663,ENST00000614327,ENST00000680316,MSTRG.7908.20,MSTRG.7908.19,MSTRG.7908.18,MSTRG.7908.25,ENST00000681698	ENST00000681704	15	+	12	103946942	103947310	103947311	103947430	103947311	103947430	103947431	103947632	14	14	1	368	119	119	201	119	284.5	120	1
ENSG00000165704:+:X:134473358:134475364	ENSG00000165704	MSTRG.34904.4,MSTRG.34904.5,ENST00000298556,MSTRG.34904.2,MSTRG.34904.3,MSTRG.34904.6,MSTRG.34904.1,MSTRG.34904.13,MSTRG.34904.10,MSTRG.34904.9,MSTRG.34904.7,MSTRG.34904.12,ENST00000462974	MSTRG.34904.4	13	+	X	134460339	134473358	134473359	134473465	134475181	134475364	134475365	134486464	2	3	2	13019	106	183	11099	144.5	12059	291	1
ENSG00000182287:-:X:15845378:15852524	ENSG00000182287	ENST00000380291,ENST00000673591,ENST00000672987,ENST00000329235,ENST00000450644,ENST00000479184,MSTRG.33927.5,ENST00000671830,ENST00000672063,ENST00000673445,ENST00000452376,MSTRG.33927.4,MSTRG.33927.3	ENST00000380291	5	-	X	15854687	15852525	15852524	15852346	15845516	15845379	15845378	15833008	2	4	3	2162	178	137	12370	157.5	7266	426	1
ENSG00000130816:-:19:10177291:10182077	ENSG00000130816	ENST00000676610,ENST00000676820,ENST00000678804,ENST00000677946,MSTRG.16395.4,ENST00000340748,ENST00000359526,ENST00000592705,MSTRG.16395.2,MSTRG.16395.8,ENST00000677013,ENST00000677634,ENST00000677250,ENST00000679103,ENST00000679313,ENST00000677685,ENST00000588118,ENST00000588952,ENST00000586988,ENST00000592054,ENST00000592342	ENST00000676610	38	-	19	10194819	10182078	10182077	10182041	10177367	10177292	10177291	10175619	2	5	4	12741	36	75	1672	55.5	7206.5	441	1
ENSG00000178028:+:1:44218194:44218755	ENSG00000178028	MSTRG.1045.3	MSTRG.1045.3	11	+	1	44214899	44218194	44218195	44218469	44218588	44218755	44218756	44219055	5	6	2	3295	274	167	299	220.5	1797	443	1
ENSG00000171824_ENSG00000116649:-:1:11055780:11066748	ENSG00000171824_ENSG00000116649	MSTRG.296.4	MSTRG.296.4	32	-	1	11068007	11066749	11066748	11066529	11055926	11055781	11055780	11055085	25	30	6	1258	219	145	695	182	976.5	818	1
ENSG00000181163:+:5:171391705:171400925	ENSG00000181163	ENST00000676625,ENST00000677467,ENST00000677672,ENST00000677600,ENST00000677741,ENST00000678280,ENST00000676504,ENST00000677904,ENST00000676589,ENST00000518587,ENST00000677357,ENST00000517671,ENST00000677325,ENST00000678774,ENST00000296930,ENST00000521672,MSTRG.27289.26,ENST00000393820,ENST00000679006	ENST00000676625	10	+	5	171391425	171391705	171391706	171391799	171400839	171400925	171400926	171405301	4	8	5	280	93	86	4375	89.5	2327.5	411	1
ENSG00000153107:-:2:111862388:111868096	ENSG00000153107	ENST00000341068,MSTRG.19062.115,MSTRG.19062.117	ENST00000341068	48	-	2	111872629	111868097	111868096	111868023	111862598	111862389	111862388	111858402	7	10	4	4532	73	209	3986	141	4259	651	1
ENSG00000137269:+:6:53878992:53913969	ENSG00000137269	ENST00000370888,MSTRG.28422.2	ENST00000370888	14	+	6	53842228	53878992	53878993	53879071	53913854	53913969	53913970	53919497	3	11	9	36764	78	115	5527	96.5	21145.5	829	1
ENSG00000113558:-:5:134157706:134167243	ENSG00000113558	MSTRG.26934.1	MSTRG.26934.1	7	-	5	134173925	134167244	134167243	134167170	134157768	134157707	134157706	134146220	3	6	4	6681	73	61	11486	67	9083.5	421	1
ENSG00000153048:-:16:8858349:8859335	ENSG00000153048	ENST00000567626	ENST00000567626	3	-	16	8860104	8859336	8859335	8859331	8859335	8859331			3	3	1	768	4	4		4	768	5	1
ENSG00000213468:-:X:131743095:131794466	ENSG00000213468	ENST00000637577,MSTRG.34881.8,MSTRG.34881.27,MSTRG.34881.11	ENST00000637577	13	-	X	131795808	131794467	131794466	131794297	131743248	131743096	131743095	131711745	2	10	9	1341	169	152	31350	160.5	16345.5	1217	1
ENSG00000105819_ENSG00000161057:+:7:103310314:103310475	ENSG00000105819_ENSG00000161057	MSTRG.30436.6	MSTRG.30436.6	25	+	7	103309096	103310314	103310315	103310475	103310315	103310475	103310476	103311642	9	9	1	1218	160	160	1166	160	1192	161	1
ENSG00000198876:-:9:34106433:34107565	ENSG00000198876	ENST00000361264,MSTRG.32564.2,ENST00000396990,ENST00000450964,MSTRG.32564.5	ENST00000361264	9	-	9	34125022	34107566	34107565	34107359	34106494	34106434	34106433	34098518	3	4	2	17456	206	60	7915	133	12685.5	268	1
ENSG00000008277:+:7:87964633:87978412	ENSG00000008277	MSTRG.30194.4,MSTRG.30194.14,MSTRG.30194.12,MSTRG.30194.9,ENST00000412441	MSTRG.30194.4	31	+	7	87935187	87964633	87964634	87964684	87978336	87978412	87978413	88075625	3	4	2	29446	50	76	97212	63	63329	128	1
ENSG00000255872:-:9:37899828:37956054	ENSG00000255872	ENST00000540557	ENST00000540557	12	-	9	37974621	37956055	37956054	37955883	37899950	37899829	37899828	37832142	5	7	3	18566	171	121	67686	146	43126	414	1
ENSG00000134884:-:13:106557047:106559657	ENSG00000134884	ENST00000472226,ENST00000400198,MSTRG.9046.4,MSTRG.9046.3,ENST00000375926	ENST00000472226	3	-	13	106567572	106559658	106559657	106557048	106559657	106557048	106557047	106544161	2	2	1	7914	2609	2609	12886	2609	10400	2610	1
ENSG00000137815:+:15:41438320:41438431	ENSG00000137815	ENST00000389629,MSTRG.10601.4,ENST00000462276,ENST00000558298	ENST00000389629	18	+	15	41417314	41438320	41438321	41438431	41438321	41438431	41438432	41452900	2	2	1	21006	110	110	14468	110	17737	111	1
ENSG00000081803:-:7:122663236:122663569	ENSG00000081803	ENST00000449022,ENST00000313070,ENST00000412584,MSTRG.30582.5	ENST00000449022	30	-	7	122736954	122663570	122663569	122663237	122663569	122663237	122663236	122629329	3	3	1	73384	332	332	33907	332	53645.5	333	1
ENSG00000133424:-:22:33761370:33761558	ENSG00000133424	ENST00000610186,ENST00000608642,ENST00000609799,ENST00000674543,ENST00000397394,ENST00000354992,ENST00000402320,ENST00000413114,ENST00000676070,ENST00000676370,ENST00000676132,ENST00000675416,ENST00000676031,ENST00000674668,ENST00000675382,ENST00000676126,ENST00000674789,ENST00000423375,ENST00000432776,ENST00000434071,ENST00000430220,ENST00000476315	ENST00000610186	13	-	22			33761558	33761371	33761558	33761371	33761370	33650669	1	1	1		187	187	110701	187	110701	188	1
ENSG00000133424:-:22:33761370:33856802	ENSG00000133424	ENST00000354992,ENST00000413114,ENST00000676132,ENST00000423375,ENST00000434071	ENST00000354992	16	-	22	33919994	33856803	33856802	33856740	33761558	33761371	33761370	33650669	2	3	2	63191	62	187	110701	124.5	86946	251	1
ENSG00000101752:+:18:21765771:21779685	ENSG00000101752	ENST00000261537,MSTRG.15558.3,MSTRG.15558.4,ENST00000578646	ENST00000261537	21	+	18	21741813	21765771	21765772	21765943	21779481	21779685	21779686	21791373	2	6	5	23958	171	204	11687	187.5	17822.5	679	1
ENSG00000062194:+:5:57214073:57214193	ENSG00000062194	ENST00000424459,MSTRG.26229.2,MSTRG.26229.10,MSTRG.26229.7,MSTRG.26229.8,MSTRG.26229.4,ENST00000506184,MSTRG.26229.9,MSTRG.26229.6,MSTRG.26229.3,MSTRG.26229.15,ENST00000511209,MSTRG.26229.14	ENST00000424459	13	+	5	57176401	57214073	57214074	57214193	57214074	57214193	57214194	57230845	3	3	1	37672	119	119	16651	119	27161.5	120	1
ENSG00000163938:+:3:52687245:52688192	ENSG00000163938	ENST00000496254,ENST00000394799,ENST00000418458,MSTRG.23062.7,MSTRG.23062.5,MSTRG.23062.11,ENST00000468146,ENST00000479230,ENST00000460073	ENST00000496254	14	+	3	52686828	52687245	52687246	52687383	52688109	52688192	52688193	52689073	3	5	3	417	137	83	880	110	648.5	336	1
ENSG00000138778:-:4:103174735:103185861	ENSG00000138778	ENST00000380026,ENST00000265148,MSTRG.25259.3,MSTRG.25259.4,MSTRG.25259.7,MSTRG.25259.6,MSTRG.25259.9,MSTRG.25259.8,MSTRG.25259.5	ENST00000380026	47	-	4	103194228	103185862	103185861	103185810	103174903	103174736	103174735	103163257	9	16	8	8366	51	167	11478	109	9922	954	1
ENSG00000109919:-:11:47625673:47627127	ENSG00000109919	ENST00000302503,MSTRG.5479.1,MSTRG.5479.7,MSTRG.5479.8,MSTRG.5479.6,MSTRG.5479.2,MSTRG.5479.15,MSTRG.5479.16,MSTRG.5479.3,ENST00000530428,MSTRG.5479.19,ENST00000534074,ENST00000525649	ENST00000302503	13	-	11	47628952	47627128	47627127	47627080	47625741	47625674	47625673	47622777	10	11	2	1824	47	67	2896	57	2360	116	1
ENSG00000110075:+:11:68505144:68596218	ENSG00000110075	ENST00000265636,MSTRG.5855.1,MSTRG.5855.3,MSTRG.5855.4,ENST00000529710	ENST00000265636	23	+	11	68460828	68505144	68505145	68505249	68596097	68596218	68596219	68600340	2	18	17	44316	104	121	4121	112.5	24218.5	2060	1
ENSG00000020426:+:14:60879713:60879835	ENSG00000020426	ENST00000261245,ENST00000539616,MSTRG.9681.5,MSTRG.9681.6,MSTRG.9681.3,MSTRG.9681.2,MSTRG.9681.1,ENST00000557134,ENST00000554002	ENST00000261245	8	+	14	60818848	60879713	60879714	60879835	60879714	60879835	60879836	60968228	7	7	1	60865	121	121	88392	121	74628.5	122	1
ENSG00000111412:-:12:116719762:116723223	ENSG00000111412	ENST00000261318,MSTRG.8092.5	ENST00000261318	5	-	12	116737789	116723224	116723223	116723067	116719876	116719763	116719762	116717894	2	4	3	14565	156	113	1868	134.5	8216.5	423	1
ENSG00000170144:+:2:177217704:177217845	ENSG00000170144	ENST00000676762,ENST00000483137,ENST00000677508,ENST00000678421,ENST00000676629,MSTRG.19566.2,ENST00000679159,ENST00000677337,ENST00000677863,ENST00000411529,MSTRG.19566.10,ENST00000676736,MSTRG.19566.30,MSTRG.19566.39,MSTRG.19566.46,MSTRG.19566.43,MSTRG.19566.24,MSTRG.19566.27,ENST00000392524,MSTRG.19566.5,ENST00000435711,MSTRG.19566.4,ENST00000679328,MSTRG.19566.12,MSTRG.19566.7,MSTRG.19566.11,MSTRG.19566.52,MSTRG.19566.42,MSTRG.19566.41,MSTRG.19566.31,MSTRG.19566.48,MSTRG.19566.44,MSTRG.19566.3,ENST00000678111,MSTRG.19566.50,MSTRG.19566.53,ENST00000676874,MSTRG.19566.25,MSTRG.19566.40,MSTRG.19566.35,MSTRG.19566.47	ENST00000676762	8	+	2	177216941	177217704	177217705	177217845	177217705	177217845	177217846	177219036	5	5	1	763	140	140	1190	140	976.5	141	1
ENSG00000182831:+:16:9102983:9103259	ENSG00000182831	ENST00000327827,MSTRG.12000.2	ENST00000327827	4	+	16	9093002	9102983	9102984	9103259	9102984	9103259	9103260	9116667	3	3	1	9981	275	275	13407	275	11694	276	1
ENSG00000137073:-:9:33971650:33973237	ENSG00000137073	ENST00000684298,ENST00000682209,ENST00000683252,ENST00000682239,ENST00000684158,MSTRG.32567.21,ENST00000379238,ENST00000681980,MSTRG.32567.14,ENST00000682914,MSTRG.32567.22,MSTRG.32567.24,ENST00000684754,MSTRG.32567.20,MSTRG.32567.26,MSTRG.32567.25,ENST00000412543,ENST00000421278	ENST00000684298	15	-	9	33986759	33973238	33973237	33973183	33971754	33971651	33971650	33963792	6	7	2	13521	54	103	7858	78.5	10689.5	159	1
ENSG00000198722:+:9:35228014:35259050	ENSG00000198722	ENST00000635942,ENST00000396787,ENST00000617908,ENST00000619578,MSTRG.32610.1,ENST00000634487,ENST00000378495	ENST00000635942	40	+	9	35162306	35228014	35228015	35228044	35258993	35259050	35259051	35295695	2	7	6	65708	29	57	36644	43	51176	504	1
ENSG00000140470:-:15:100254135:100281401	ENSG00000140470	ENST00000268070,MSTRG.11651.2,MSTRG.11651.4,MSTRG.11651.5,ENST00000568565,ENST00000378898	ENST00000268070	22	-	15	100330888	100281402	100281401	100281229	100254179	100254136	100254135	100199424	4	7	4	49486	172	43	54711	107.5	52098.5	459	1
ENSG00000115241:-:2:27384672:27384714	ENSG00000115241	MSTRG.18123.18	MSTRG.18123.18	11	-	2	27384789	27384715	27384714	27384673	27384714	27384673	27384672	27384093	6	6	1	74	41	41	579	41	326.5	42	1
ENSG00000107560:-:10:118009081:118015110	ENSG00000107560	MSTRG.4703.4,MSTRG.4703.3,ENST00000476207	MSTRG.4703.4	6	-	10	118038971	118015111	118015110	118015065	118009225	118009082	118009081	118005839	4	5	2	23860	45	143	3242	94	13551	190	1
ENSG00000156931:+:3:184964467:184999861	ENSG00000156931	ENST00000287546,MSTRG.24207.6,ENST00000625842,ENST00000436792,ENST00000446204,ENST00000492449	ENST00000287546	47	+	3	184957522	184964467	184964468	184964557	184999696	184999861	184999862	185024335	37	44	8	6945	89	165	24473	127	15709	819	1
ENSG00000176715:+:16:89098590:89133262	ENSG00000176715	MSTRG.13252.9,ENST00000614302,ENST00000317447,MSTRG.13252.16,MSTRG.13252.10,MSTRG.13252.13	MSTRG.13252.9	11	+	16	89093997	89098590	89098591	89098763	89133136	89133262	89133263	89145213	2	8	7	4593	172	126	11950	149	8271.5	1559	1
ENSG00000110435:+:11:34931403:34970286	ENSG00000110435	ENST00000448838,ENST00000227868,MSTRG.5375.3	ENST00000448838	11	+	11	34916487	34931403	34931404	34931484	34970139	34970286	34970287	34978123	2	7	6	14916	80	147	7836	113.5	11376	804	1
ENSG00000037637:-:1:16294782:16315435	ENSG00000037637	ENST00000375592,MSTRG.396.2,MSTRG.396.3,MSTRG.396.11,MSTRG.396.9,MSTRG.396.7,MSTRG.396.6,MSTRG.396.10	ENST00000375592	10	-	1	16352254	16315436	16315435	16315169	16294917	16294783	16294782	16256760	2	4	3	36818	266	134	38022	200	37420	519	1
ENSG00000169062:+:13:114286518:114291792	ENSG00000169062	ENST00000375299,MSTRG.9117.12,ENST00000351487,MSTRG.9117.2,MSTRG.9117.8,ENST00000480362,ENST00000475218,ENST00000493727,ENST00000481131,ENST00000484246	ENST00000375299	10	+	13	114286401	114286518	114286519	114286629	114291634	114291792	114291793	114298839	5	7	3	117	110	158	7046	134	3581.5	326	1
ENSG00000242259_ENSG00000070010:-:22:19447377:19456918	ENSG00000242259_ENSG00000070010	MSTRG.21629.23,MSTRG.21629.24	MSTRG.21629.23	15	-	22	19458070	19456919	19456918	19456853	19447545	19447378	19447377	19444391	8	13	6	1151	65	167	2986	116	2068.5	3533	1
ENSG00000099942:+:22:20933778:20934244	ENSG00000099942	ENST00000354336,MSTRG.21687.3,ENST00000411769	ENST00000354336	3	+	22	20918246	20933778	20933779	20934244	20933779	20934244	20934245	20949710	2	2	1	15532	465	465	15465	465	15498.5	466	1
ENSG00000131747:-:17:40391505:40411818	ENSG00000131747	ENST00000423485,MSTRG.14296.3	ENST00000423485	35	-	17	40412758	40411819	40411818	40411645	40391640	40391506	40391505	40390165	8	33	26	939	173	134	1340	153.5	1139.5	3478	1
ENSG00000171863_ENSG00000234171:+:2:3575591:3576630	ENSG00000171863_ENSG00000234171	MSTRG.17912.8	MSTRG.17912.8	9	+	2	3575351	3575591	3575592	3575684	3576487	3576630	3576631	3577709	4	6	3	240	92	143	1078	117.5	659	309	1
ENSG00000117523:+:1:171523220:171537473	ENSG00000117523	MSTRG.2588.1,MSTRG.2588.5,ENST00000647382,MSTRG.2588.15,MSTRG.2588.3,MSTRG.2588.8,MSTRG.2588.16,MSTRG.2588.6,MSTRG.2588.10,MSTRG.2588.18,ENST00000367742,ENST00000338920,MSTRG.2588.9,MSTRG.2588.2,MSTRG.2588.14,MSTRG.2588.7,MSTRG.2588.19,MSTRG.2588.20,ENST00000426496	MSTRG.2588.1	35	+	1	171522260	171523220	171523221	171523354	171537263	171537473	171537474	171539970	8	15	8	960	133	210	2496	171.5	1728	1665	1
ENSG00000241962:+:2:99186176:99188257	ENSG00000241962	ENST00000424491	ENST00000424491	14	+	2	99150520	99186176	99186177	99186254	99188177	99188257	99188258	99194750	5	6	2	35656	77	80	6492	78.5	21074	159	1
ENSG00000171103:-:2:28850327:28852499	ENSG00000171103	ENST00000306108,MSTRG.18153.5,MSTRG.18153.6,MSTRG.18153.9,MSTRG.18153.7,MSTRG.18153.8,ENST00000439947,MSTRG.18153.1	ENST00000306108	7	-	2	28861117	28852500	28852499	28852408	28850405	28850328	28850327	28850243	4	6	3	8617	91	77	84	84	4350.5	397	1
ENSG00000101868:+:X:24888005:24888122	ENSG00000101868	ENST00000611764,ENST00000379068,ENST00000379059,ENST00000672178,ENST00000679301	ENST00000611764	24	+	X	24843678	24888005	24888006	24888122	24888006	24888122	24888123	24930452	22	22	1	44327	116	116	42329	116	43328	117	1
ENSG00000271793:-:6:85636966:85637356	ENSG00000271793	ENST00000682106,ENST00000682083,ENST00000683074,ENST00000682504,ENST00000681965,ENST00000683364,ENST00000684220,ENST00000684462,ENST00000683436	ENST00000682106	44	-	6	85640220	85637357	85637356	85637243	85637143	85636967	85636966	85624113	9	10	2	2863	113	176	12853	144.5	7858	291	1
ENSG00000139514:-:13:29553760:29553860	ENSG00000139514	ENST00000380752,MSTRG.8545.2,MSTRG.8545.4,ENST00000450494	ENST00000380752	13	-	13	29595415	29553861	29553860	29553761	29553860	29553761	29553760	29536203	2	2	1	41554	99	99	17557	99	29555.5	100	1
ENSG00000089597:-:11:62626334:62626440	ENSG00000089597	ENST00000346178,MSTRG.5609.8,MSTRG.5609.9,ENST00000648273,MSTRG.5609.6,ENST00000356638,MSTRG.5609.12,MSTRG.5609.10,MSTRG.5609.13,ENST00000532402,ENST00000540933,MSTRG.5609.11,MSTRG.5609.15,MSTRG.5609.14,MSTRG.5609.16,MSTRG.5609.17,ENST00000534779,ENST00000528503	ENST00000346178	25	-	11	62626570	62626448	62626447	62626335	62626447	62626335	62626334	62626166	23	23	1	122	112	112	168	112	145	113	1
ENSG00000092470:+:15:43835060:43851245	ENSG00000092470	ENST00000263795,MSTRG.10703.8,MSTRG.10703.5,MSTRG.10703.2,ENST00000381246,MSTRG.10703.7,MSTRG.10703.1,MSTRG.10703.11,MSTRG.10703.10,MSTRG.10703.4	ENST00000263795	13	+	15	43828367	43835060	43835061	43835150	43851087	43851245	43851246	43856945	3	9	7	6693	89	158	5699	123.5	6196	729	1
ENSG00000048471:+:16:12477718:12477859	ENSG00000048471	ENST00000566228,ENST00000564791	ENST00000566228	21	+	16	12403530	12477718	12477719	12477859	12477719	12477859	12477860	12524701	19	19	1	74188	140	140	46841	140	60514.5	141	1
ENSG00000166441:+:11:8683201:8684892	ENSG00000166441	ENST00000314138,MSTRG.5123.1,ENST00000534599,ENST00000524496,ENST00000526562,ENST00000532359	ENST00000314138	5	+	11	8682817	8683201	8683202	8683265	8684718	8684892	8684893	8685677	2	4	3	384	63	174	784	118.5	584	315	1
ENSG00000099203:-:19:10834933:10836430	ENSG00000099203	MSTRG.16429.1,ENST00000214869,ENST00000591157,ENST00000588259,ENST00000589638	MSTRG.16429.1	5	-	19	10836533	10836431	10836430	10836009	10835117	10834934	10834933	10833214	2	4	3	102	421	183	1719	302	910.5	704	1
ENSG00000110911:-:12:50990794:51010766	ENSG00000110911	ENST00000394904,ENST00000262052,ENST00000646740,ENST00000642227,ENST00000547688,ENST00000643884,ENST00000644495,MSTRG.7297.8,MSTRG.7297.9,ENST00000541174,ENST00000547198,ENST00000546636,MSTRG.7297.2,MSTRG.7297.7,ENST00000546743	ENST00000394904	16	-	12	51028176	51010767	51010766	51010695	50990948	50990795	50990794	50988436	2	15	14	17409	71	153	2358	112	9883.5	1613	1
ENSG00000223573_ENSG00000130254:-:19:5591747:5595497	ENSG00000223573_ENSG00000130254	MSTRG.16244.7,MSTRG.16244.5,MSTRG.16244.6,MSTRG.16244.3,MSTRG.16244.10	MSTRG.16244.7	22	-	19	5598792	5595498	5595497	5595361	5591793	5591748	5591747	5590409	14	17	4	3294	136	45	1338	90.5	2316	612	1
ENSG00000171045:-:8:142330900:142354763	ENSG00000171045	MSTRG.32164.6,MSTRG.32164.7,MSTRG.32164.8,ENST00000519651,MSTRG.32164.12,MSTRG.32164.14,MSTRG.32164.5,ENST00000307180,ENST00000524325,ENST00000662555,MSTRG.32164.9,ENST00000520166,MSTRG.32164.11	MSTRG.32164.6	14	-	8	142403103	142354764	142354763	142354637	142330970	142330901	142330900	142318635	2	6	5	48339	126	69	12265	97.5	30302	932	1
ENSG00000197157:+:7:127686612:127721400	ENSG00000197157	ENST00000354725,MSTRG.30622.1,MSTRG.30622.5,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.6	ENST00000354725	24	+	7	127652452	127686612	127686613	127686762	127721287	127721400	127721401	127807483	2	10	9	34160	149	113	86082	131	60121	1074	1
ENSG00000181090_ENSG00000197070:+:9:137612895:137619049	ENSG00000181090_ENSG00000197070	MSTRG.33733.4,MSTRG.33733.1,MSTRG.33733.2	MSTRG.33733.4	31	+	9	137605836	137612895	137612896	137613510	137615074	137619049	137619050	137716625	2	7	6	7059	614	3975	97575	2294.5	52317	5548	1
ENSG00000130741:+:X:24062415:24066092	ENSG00000130741	ENST00000253039,MSTRG.34026.2,MSTRG.34026.6,MSTRG.34026.12,MSTRG.34026.4,MSTRG.34026.5,MSTRG.34026.7,MSTRG.34026.11	ENST00000253039	12	+	X	24060183	24062415	24062416	24062574	24065998	24066092	24066093	24067963	6	8	3	2232	158	94	1870	126	2051	389	1
ENSG00000165671:+:5:177209635:177239865	ENSG00000165671	ENST00000439151,MSTRG.27376.5,ENST00000687453,ENST00000685206,MSTRG.27376.12,ENST00000688613,ENST00000354179,ENST00000689345,ENST00000686993,ENST00000347982,ENST00000689549,ENST00000689326,MSTRG.27376.8	ENST00000439151	23	+	5	177204293	177209635	177209636	177212195	177239756	177239865	177239866	177244194	5	8	4	5342	2559	109	4328	1334	4835	3066	1
ENSG00000103342:-:16:11875760:11898035	ENSG00000103342	ENST00000434724,MSTRG.12076.3,ENST00000439887,ENST00000420576	ENST00000434724	15	-	16	11915368	11898036	11898035	11897994	11875929	11875761	11875760	11873172	2	14	13	17332	41	168	2588	104.5	9960	1509	1
ENSG00000089006:-:20:17947485:17957037	ENSG00000089006	ENST00000483485,MSTRG.20408.9,MSTRG.20408.4,ENST00000377759,MSTRG.20408.8,ENST00000377768,MSTRG.20408.13,ENST00000490175,MSTRG.20408.11,MSTRG.20408.14,MSTRG.20408.12	ENST00000483485	14	-	20	17961139	17957038	17957037	17956933	17947645	17947486	17947485	17943196	2	12	11	4101	104	159	4289	131.5	4195	1334	1
ENSG00000111358:+:12:123645454:123652735	ENSG00000111358	MSTRG.8256.3,MSTRG.8256.1,ENST00000543341,ENST00000543415,ENST00000618160,ENST00000228955,ENST00000536375,ENST00000538845,ENST00000538533,ENST00000537695,ENST00000539994,ENST00000542279	MSTRG.8256.3	13	+	12	123639344	123645454	123645455	123645561	123652707	123652735	123652736	123654923	3	7	5	6110	106	28	2187	67	4148.5	393	1
ENSG00000185721:+:22:31403028:31411081	ENSG00000185721	MSTRG.21971.1,ENST00000331457,MSTRG.21971.5,ENST00000433341,ENST00000486584	MSTRG.21971.1	11	+	22	31400744	31403028	31403029	31403204	31411012	31411081	31411082	31420255	3	4	2	2284	175	69	9173	122	5728.5	246	1
ENSG00000131467:+:17:42838730:42839380	ENSG00000131467	ENST00000545225,ENST00000441946,ENST00000590720,ENST00000543428,ENST00000592169	ENST00000545225	13	+	17	42838206	42838730	42838731	42838799	42839294	42839380	42839381	42841497	9	12	4	524	68	86	2116	77	1320	279	1
ENSG00000058091:+:7:90726566:90726812	ENSG00000058091	ENST00000380050,ENST00000406263,ENST00000265741,ENST00000436577,ENST00000431029,ENST00000449528,ENST00000496279,ENST00000456689,ENST00000446224,ENST00000446790,ENST00000430760	ENST00000380050	15	+	7	90604250	90726566	90726567	90726812	90726567	90726812	90726813	90747680	3	3	1	122316	245	245	20867	245	71591.5	246	1
ENSG00000169379:+:3:93995873:94003908	ENSG00000169379	ENST00000679872,ENST00000679404,ENST00000679601,ENST00000681380,ENST00000335438,ENST00000680414,ENST00000681655,MSTRG.23316.22,ENST00000394222,ENST00000680430,ENST00000681013,ENST00000471138,ENST00000679587,MSTRG.23316.2	ENST00000679872	12	+	3	93989117	93995873	93995874	93995944	94003659	94003908	94003909	94035330	4	5	2	6756	70	249	31421	159.5	19088.5	321	1
ENSG00000131018:-:6:152201823:152215060	ENSG00000131018	ENST00000367255,ENST00000423061,ENST00000367256,ENST00000409694,MSTRG.29204.16,ENST00000367251,ENST00000367257	ENST00000367255	146	-	6	152218256	152215061	152215060	152214906	152201949	152201824	152201823	152189408	122	127	6	3195	154	125	12415	139.5	7805	954	1
ENSG00000120860:-:12:102039867:102046119	ENSG00000120860	ENST00000538761,ENST00000240079,ENST00000535501,ENST00000540536,ENST00000544341,ENST00000545679,ENST00000539515,ENST00000541569	ENST00000538761	8	-	12	102048289	102046120	102046119	102046054	102039978	102039868	102039867	102026039	4	6	3	2169	65	110	13828	87.5	7998.5	285	1
ENSG00000196912:-:2:97549418:97551480	ENSG00000196912	ENST00000258459,ENST00000359901,ENST00000443455	ENST00000258459	44	-	2	97553167	97551481	97551480	97551452	97549491	97549419	97549418	97547732	17	20	4	1686	28	72	1686	50	1686	204	1
ENSG00000169402:+:7:6759083:6768766	ENSG00000169402	ENST00000403107,ENST00000404077,ENST00000297186	ENST00000403107	20	+	7	6757932	6759083	6759084	6759159	6768590	6768766	6768767	6771546	3	8	6	1151	75	176	2779	125.5	1965	703	1
ENSG00000145907:+:5:151786571:151803996	ENSG00000145907	MSTRG.27198.12	MSTRG.27198.12	14	+	5	151772037	151786571	151786572	151786715	151803885	151803996	151803997	151804405	2	12	11	14534	143	111	408	127	7471	1355	1
ENSG00000181163:+:5:171391304:171407774	ENSG00000181163	ENST00000676625,ENST00000678267,ENST00000676613,ENST00000678186,ENST00000677467,ENST00000677672,ENST00000677600,ENST00000678280,ENST00000676504,ENST00000521260,ENST00000677904,ENST00000676589,ENST00000518587,ENST00000677357,ENST00000517671,ENST00000677325,ENST00000678774,ENST00000296930,ENST00000521672,ENST00000351986,ENST00000677907,MSTRG.27289.27,ENST00000677297,ENST00000679190	ENST00000676625	10	+	5	171390131	171391304	171391305	171391424	171405302	171407774	171407775	171410526	3	9	7	1173	119	2472	2751	1295.5	1962	3004	1
ENSG00000053900:+:4:25396718:25396764	ENSG00000053900	ENST00000505842	ENST00000505842	8	+	4			25396719	25396764	25396719	25396764	25396765	25396847	1	1	1		45	45	82	45	82	46	1
ENSG00000219481:-:1:16579192:16583696	ENSG00000219481	ENST00000430580,MSTRG.409.2,MSTRG.409.3,MSTRG.409.4,MSTRG.409.8,MSTRG.409.10,MSTRG.409.9	ENST00000430580	29	-	1	16585488	16583697	16583696	16583594	16579402	16579193	16579192	16577420	13	17	5	1791	102	209	1772	155.5	1781.5	813	1
ENSG00000116273:+:1:6619802:6620337	ENSG00000116273	ENST00000377648,MSTRG.198.3	ENST00000377648	4	+	1	6616859	6619802	6619803	6620337	6619803	6620337	6620338	6621410	3	3	1	2943	534	534	1072	534	2007.5	535	1
ENSG00000164535:-:7:6432836:6436533	ENSG00000164535	MSTRG.29469.1,ENST00000297056,MSTRG.29469.2,ENST00000436575,ENST00000425398	MSTRG.29469.1	15	-	7	6445952	6436534	6436533	6436362	6432959	6432837	6432836	6430608	3	5	3	9418	171	122	2228	146.5	5823	522	1
ENSG00000288622:+:5:314416:428006	ENSG00000288622	MSTRG.25844.34	MSTRG.25844.34	16	+	5	311403	314416	314417	314596	427807	428006	428007	432462	6	13	8	3013	179	199	4455	189	3734	1098	1
ENSG00000196284:-:6:45020545:45020632	ENSG00000196284	ENST00000371459,ENST00000674231,ENST00000371460,ENST00000475057,MSTRG.28371.3,ENST00000637763	ENST00000371459	11	-	6	45105921	45020633	45020632	45020546	45020632	45020546	45020545	45014892	4	4	1	85288	86	86	5653	86	45470.5	87	1
ENSG00000189283:-:3:60821918:60822011	ENSG00000189283	ENST00000492590,ENST00000490952,ENST00000468189,ENST00000476844,MSTRG.23171.3,MSTRG.23171.4,ENST00000488467	ENST00000492590	10	-	3	61042046	60822012	60822011	60821919	60822011	60821919	60821918	60536980	4	4	1	220034	92	92	284938	92	252486	93	1
ENSG00000107829_ENSG00000224817:-:10:101667885:101673673	ENSG00000107829_ENSG00000224817	MSTRG.4529.1	MSTRG.4529.1	10	-	10	101676340	101673674	101673673	101673488	101667980	101667886	101667885	101624811	4	6	3	2666	185	94	43074	139.5	22870	414	1
ENSG00000159140:+:21:33567156:33569087	ENSG00000159140	MSTRG.21290.1,ENST00000455528,ENST00000356577,MSTRG.21290.12,MSTRG.21290.11,ENST00000436227,MSTRG.21290.16,MSTRG.21290.15,ENST00000695559,ENST00000381692	MSTRG.21290.1	12	+	21	33559776	33567156	33567157	33567267	33568971	33569087	33569088	33573307	7	8	2	7380	110	116	4219	113	5799.5	228	1
ENSG00000183570:+:21:45735391:45755452	ENSG00000183570	ENST00000681687,MSTRG.21495.1,MSTRG.21495.2,MSTRG.21495.7,MSTRG.21495.10	ENST00000681687	18	+	21	45668953	45735391	45735392	45735429	45755417	45755452	45755453	45849960	3	4	2	66438	37	35	94507	36	80472.5	74	1
ENSG00000072736:+:16:68121986:68123121	ENSG00000072736	ENST00000535127,ENST00000563319,ENST00000569766,ENST00000349223,ENST00000346183,ENST00000566301,ENST00000329524,ENST00000563288,ENST00000563796,ENST00000562926,ENST00000567152,ENST00000570212,ENST00000553077,ENST00000575270,ENST00000549350,ENST00000379165	ENST00000535127	11	+	16	68089203	68121986	68121987	68123121	68121987	68123121	68123122	68126447	2	2	1	32783	1134	1134	3325	1134	18054	1135	1
ENSG00000164080_ENSG00000164081:+:3:51674270:51674562	ENSG00000164080_ENSG00000164081	MSTRG.23022.4,MSTRG.23022.3,MSTRG.23022.6,MSTRG.23022.1,MSTRG.23022.7,MSTRG.23022.11	MSTRG.23022.4	26	+	3	51662877	51674270	51674271	51674562	51674271	51674562	51674563	51684412	23	23	1	11393	291	291	9849	291	10621	292	1
ENSG00000198815:-:1:42278347:42311110	ENSG00000198815	ENST00000372572,ENST00000372573,ENST00000361346,ENST00000545068,ENST00000361776,MSTRG.976.7,ENST00000445886,MSTRG.976.10,MSTRG.976.9	ENST00000372572	15	-	1	42315345	42311111	42311110	42311050	42278672	42278348	42278347	42265190	4	5	2	4234	60	324	13157	192	8695.5	386	1
ENSG00000117262:+:1:145616233:145647267	ENSG00000117262	MSTRG.2007.5,ENST00000460277,ENST00000313835,MSTRG.2007.4,MSTRG.2007.1,ENST00000534502,ENST00000462900,MSTRG.2007.9,ENST00000528944	MSTRG.2007.5	15	+	1	145608176	145616233	145616234	145616293	145647175	145647267	145647268	145663328	2	11	10	8057	59	92	16060	75.5	12058.5	944	1
ENSG00000197157:+:7:127844323:127991056	ENSG00000197157	ENST00000354725,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.2,MSTRG.30622.4	ENST00000354725	24	+	7	127807574	127844323	127844324	127844424	127990947	127991056	127991057	128074501	12	16	5	36749	100	109	83444	104.5	60096.5	537	1
ENSG00000106443:+:7:10982371:10983159	ENSG00000106443	ENST00000403050,ENST00000490957,ENST00000634607,MSTRG.29511.9	ENST00000403050	17	+	7	10974946	10982371	10982372	10983159	10982372	10983159	10983160	10990702	3	3	1	7425	787	787	7542	787	7483.5	788	1
ENSG00000234545:-:7:92569822:92581603	ENSG00000234545	ENST00000438306,ENST00000445716,ENST00000481407,ENST00000415397,MSTRG.30217.5,ENST00000427372,MSTRG.30217.6	ENST00000438306	12	-	7	92585297	92581604	92581603	92581506	92569915	92569823	92569822	92566062	3	10	8	3693	97	92	3760	94.5	3726.5	585	1
ENSG00000204628:-:5:181238098:181238239	ENSG00000204628	MSTRG.27493.12,ENST00000513060,MSTRG.27493.2,MSTRG.27493.1,ENST00000508682,MSTRG.27493.22,ENST00000511473,ENST00000512805,MSTRG.27493.13,MSTRG.27493.21,MSTRG.27493.23,ENST00000512968,ENST00000504325,ENST00000376817,ENST00000504128,MSTRG.27493.6,ENST00000511900,ENST00000507756,ENST00000502905,ENST00000509535	MSTRG.27493.12	9	-	5	181239066	181238240	181238239	181238099	181238239	181238099	181238098	181237720	7	7	1	826	140	140	378	140	602	141	1
ENSG00000137154:-:9:19378367:19378506	ENSG00000137154	MSTRG.32455.7,MSTRG.32455.13	MSTRG.32455.7	6	-	9	19378707	19378507	19378506	19378368	19378506	19378368	19378367	19376652	4	4	1	200	138	138	1715	138	957.5	139	1
ENSG00000171843:-:9:20413720:20414425	ENSG00000171843	ENST00000380338,ENST00000475957,ENST00000630269	ENST00000380338	11	-	9	20448122	20414426	20414425	20413721	20414425	20413721	20413720	20365745	5	5	1	33696	704	704	47975	704	40835.5	705	1
ENSG00000198604:-:14:34792774:34862322	ENSG00000198604	ENST00000360310,ENST00000358716,ENST00000382422,MSTRG.9366.7,MSTRG.9366.5,MSTRG.9366.6	ENST00000360310	27	-	14	34874491	34862323	34862322	34862044	34792921	34792775	34792774	34786222	3	12	10	12168	278	146	6552	212	9360	1397	1
ENSG00000099783:+:19:8455404:8463686	ENSG00000099783	MSTRG.16353.1,MSTRG.16353.2,ENST00000348943,ENST00000325495,ENST00000620401,ENST00000600806,ENST00000600092,ENST00000594907,ENST00000601645,ENST00000596984	MSTRG.16353.1	15	+	19	8445112	8455404	8455405	8455574	8463593	8463686	8463687	8465323	2	5	4	10292	169	93	1636	131	5964	325	1
ENSG00000168488:+:16:28825623:28832567	ENSG00000168488	ENST00000563314,ENST00000336783,ENST00000395547,MSTRG.12366.28,ENST00000564304,ENST00000340394,ENST00000325215,ENST00000382686,MSTRG.12366.13,ENST00000570200,MSTRG.12366.19,MSTRG.12366.17,MSTRG.12366.15,MSTRG.12366.16,MSTRG.12366.20,MSTRG.12366.22,MSTRG.12366.18,MSTRG.12366.21,MSTRG.12366.10,MSTRG.12366.9,MSTRG.12366.25,MSTRG.12366.24,MSTRG.12366.29,MSTRG.12366.11	ENST00000563314	21	+	16	28825403	28825623	28825624	28825680	28832496	28832567	28832568	28832816	3	12	10	220	56	71	248	63.5	234	1252	1
ENSG00000064419:-:7:129014978:129018157	ENSG00000064419	ENST00000627585,ENST00000265388,MSTRG.30659.3,ENST00000482320,ENST00000471234,ENST00000471166	ENST00000627585	23	-	7	129054650	129018158	129018157	129017957	129015135	129014979	129014978	129005160	2	4	3	36492	200	156	9818	178	23155	432	1
ENSG00000095951:+:6:12015525:12015668	ENSG00000095951	ENST00000379388,ENST00000541134,ENST00000627968,MSTRG.27632.5,ENST00000491710,ENST00000478545,ENST00000487103,MSTRG.27632.4,ENST00000442081	ENST00000379388	9	+	6	12012567	12015525	12015526	12015668	12015526	12015668	12015669	12089183	2	2	1	2958	142	142	73514	142	38236	143	1
ENSG00000144040:-:2:73020238:73040931	ENSG00000144040	ENST00000474528,ENST00000272433,ENST00000461352,ENST00000450185,MSTRG.18653.4,MSTRG.18653.16,ENST00000411783,MSTRG.18653.15,MSTRG.18653.13,ENST00000416579,ENST00000497144,ENST00000410065,ENST00000464825,ENST00000487508,ENST00000442582	ENST00000474528	14	-	2	73058527	73040932	73040931	73040854	73020264	73020239	73020238	73001579	3	6	4	17595	77	25	18659	51	18127	186	1
ENSG00000113269:-:5:180040452:180040647	ENSG00000113269	ENST00000522208,ENST00000521389,MSTRG.27444.2,ENST00000261947,MSTRG.27444.6,MSTRG.27444.5,MSTRG.27444.11	ENST00000522208	8	-	5	180071455	180040648	180040647	180040453	180040647	180040453	180040452	180013312	2	2	1	30807	194	194	27140	194	28973.5	195	1
ENSG00000156299:-:21:31135932:31141504	ENSG00000156299	ENST00000286827,MSTRG.21214.6,ENST00000541036,MSTRG.21214.5,MSTRG.21214.13,MSTRG.21214.11,MSTRG.21214.9,MSTRG.21214.10,MSTRG.21214.8,MSTRG.21214.12,MSTRG.21214.14,MSTRG.21214.15,ENST00000455508,ENST00000636887	ENST00000286827	29	-	21	31146894	31141505	31141504	31141325	31136041	31135933	31135932	31130949	22	24	3	5389	179	108	4983	143.5	5186	408	1
ENSG00000132356:-:5:40764513:40777586	ENSG00000132356	ENST00000397128,ENST00000354209	ENST00000397128	9	-	5	40798062	40777587	40777586	40777445	40764640	40764514	40764513	40763023	2	8	7	20475	141	126	1490	133.5	10982.5	1308	1
ENSG00000029725:+:17:5308693:5308822	ENSG00000029725	MSTRG.13506.1,ENST00000537505,MSTRG.13506.8,MSTRG.13506.6,MSTRG.13506.10,MSTRG.13506.5,ENST00000341923,MSTRG.13506.4,ENST00000575991	MSTRG.13506.1	19	+	17	5284070	5308693	5308694	5308822	5308694	5308822	5308823	5331948	3	3	1	24623	128	128	23125	128	23874	129	1
ENSG00000105821:-:7:103321931:103322794	ENSG00000105821	ENST00000379263,MSTRG.30437.5,ENST00000464253,ENST00000249270,ENST00000475065	ENST00000379263	17	-	7	103323597	103322795	103322794	103322703	103322081	103321932	103321931	103319845	8	10	3	802	91	149	2086	120	1444	364	1
ENSG00000164933:-:8:103404775:103407784	ENSG00000164933	ENST00000297578,ENST00000521645,MSTRG.31960.7,MSTRG.31960.6	ENST00000297578	7	-	8	103414783	103407785	103407784	103407634	103404861	103404776	103404775	103403325	2	3	2	6998	150	85	1450	117.5	4224	237	1
ENSG00000165914:-:14:90676522:90680535	ENSG00000165914	ENST00000328459,MSTRG.10070.2,ENST00000555005,MSTRG.10070.8,MSTRG.10070.6,ENST00000555239	ENST00000328459	20	-	14	90689539	90680536	90680535	90680472	90676660	90676523	90676522	90658388	8	9	2	9003	63	137	18134	100	13568.5	202	1
ENSG00000009307:-:1:114719578:114725333	ENSG00000009307	ENST00000690784,ENST00000686581,ENST00000693644,ENST00000687548,ENST00000688211,ENST00000692950,ENST00000610726,ENST00000689217,ENST00000689989,ENST00000438362,ENST00000691403,ENST00000684913,ENST00000686123,ENST00000358528,ENST00000689732,ENST00000686235,ENST00000693467,ENST00000690013,ENST00000685676,ENST00000690207,ENST00000693311,ENST00000690420,ENST00000339438,ENST00000686025,ENST00000692672,ENST00000692629,ENST00000689885,ENST00000692044,ENST00000686781,ENST00000692719,ENST00000689510,ENST00000688611,ENST00000691032,ENST00000369530,ENST00000687759,ENST00000686667,ENST00000691377,ENST00000530886,ENST00000529046,ENST00000261443,MSTRG.1873.45,ENST00000689631,ENST00000534699,MSTRG.1873.47,MSTRG.1873.48	ENST00000690784	19	-	1	114726210	114725334	114725333	114725221	114720717	114719579	114719578	114718746	15	17	3	876	112	1138	832	625	854	1372	1
ENSG00000090273:+:1:26934248:26934419	ENSG00000090273	MSTRG.630.2	MSTRG.630.2	10	+	1	26924167	26934248	26934249	26934419	26934249	26934419	26934420	26941456	3	3	1	10081	170	170	7036	170	8558.5	171	1
ENSG00000061938:-:3:195872275:195888606	ENSG00000061938	ENST00000333602,MSTRG.24313.1,ENST00000672887,ENST00000673420,ENST00000673038,ENST00000672024,ENST00000428187,ENST00000671753,ENST00000381916,MSTRG.24313.9,MSTRG.24313.15,MSTRG.24313.13,MSTRG.24313.14,ENST00000673374,ENST00000439230	ENST00000333602	15	-	3	195908484	195888607	195888606	195888426	195872470	195872276	195872275	195870206	2	10	9	19877	180	194	2069	187	10973	1469	1
ENSG00000101868:+:X:24809897:24815111	ENSG00000101868	ENST00000611764,ENST00000379068,ENST00000379059,ENST00000672178,MSTRG.34044.1,ENST00000679301	ENST00000611764	24	+	X	24748993	24809897	24809898	24809930	24814979	24815111	24815112	24821451	14	17	4	60904	32	132	6339	82	33621.5	465	1
ENSG00000107854:+:10:91848382:91851336	ENSG00000107854	MSTRG.4372.2,ENST00000371627,MSTRG.4372.3,MSTRG.4372.4,MSTRG.4372.5	MSTRG.4372.2	27	+	10	91845941	91848382	91848383	91848635	91851216	91851336	91851337	91855028	19	21	3	2441	252	120	3691	186	3066	457	1
ENSG00000102181:-:X:150814861:150831293	ENSG00000102181	ENST00000346693,ENST00000613030,ENST00000370377,ENST00000437787,MSTRG.35038.5,ENST00000418547	ENST00000346693	12	-	X	150898521	150831294	150831293	150831231	150814936	150814862	150814861	150795487	2	5	4	67227	62	74	19374	68	43300.5	1509	1
ENSG00000189091:+:16:70532478:70569141	ENSG00000189091	ENST00000302516,MSTRG.12933.3,MSTRG.12933.1,MSTRG.12933.4,MSTRG.12933.5,MSTRG.12933.7,MSTRG.12933.6	ENST00000302516	26	+	16	70530918	70532478	70532479	70532620	70569043	70569141	70569142	70570005	5	23	19	1560	141	98	863	119.5	1211.5	2694	1
ENSG00000086189:-:5:62390875:62394607	ENSG00000086189	ENST00000680267,ENST00000651114,ENST00000509182,ENST00000681244,ENST00000199320,ENST00000680785,ENST00000680214,MSTRG.26267.3,ENST00000680062,ENST00000681393,ENST00000680960,ENST00000681192,ENST00000681672,ENST00000679751,ENST00000681272	ENST00000680267	9	-	5	62398510	62394608	62394607	62394484	62392990	62390876	62390875	62389053	6	8	3	3902	123	2114	1822	1118.5	2862	2332	1
ENSG00000158987:-:5:131548046:131548190	ENSG00000158987	ENST00000509018,ENST00000507093,ENST00000296859,ENST00000627212,ENST00000308008,ENST00000515170,ENST00000510071	ENST00000509018	28	-	5	131561977	131548191	131548190	131548047	131548190	131548047	131548046	131521522	6	6	1	13786	143	143	26524	143	20155	144	1
ENSG00000141568:+:17:82563353:82571870	ENSG00000141568	ENST00000335255,ENST00000473637,MSTRG.15360.4,ENST00000526383,ENST00000531030	ENST00000335255	9	+	17	82520308	82563353	82563354	82563548	82571724	82571870	82571871	82582740	2	4	3	43045	194	146	10869	170	26957	490	1
ENSG00000130313:+:19:17516172:17517850	ENSG00000130313	ENST00000594761,MSTRG.16692.2,ENST00000252603,ENST00000600923	ENST00000594761	6	+	19	17511961	17516172	17516173	17516280	17517710	17517850	17517851	17520943	2	5	4	4211	107	140	3092	123.5	3651.5	455	1
ENSG00000002822:-:7:1980452:2149238	ENSG00000002822	ENST00000399654,ENST00000406869,ENST00000265854,MSTRG.29392.10,MSTRG.29392.15,ENST00000402746,MSTRG.29392.11,MSTRG.29392.12,MSTRG.29392.14,MSTRG.29392.13	ENST00000399654	19	-	7	2213211	2149239	2149238	2149152	1980541	1980453	1980452	1957720	11	15	5	63972	86	88	22732	87	43352	519	1
ENSG00000124571:-:6:43560177:43561007	ENSG00000124571	ENST00000265351,MSTRG.28337.3,MSTRG.28337.4,ENST00000496341	ENST00000265351	32	-	6	43562246	43561008	43561007	43560924	43560303	43560178	43560177	43558592	10	11	2	1238	83	125	1585	104	1411.5	210	1
ENSG00000146555:+:7:4049347:4114274	ENSG00000146555	ENST00000404826,MSTRG.29412.5,ENST00000389531,MSTRG.29412.4,MSTRG.29412.3	ENST00000404826	45	+	7	4017353	4049347	4049348	4049463	4114037	4114274	4114275	4127380	18	25	8	31994	115	237	13105	176	22549.5	1221	1
ENSG00000168763:+:2:96825057:96829134	ENSG00000168763	MSTRG.18891.3,MSTRG.18891.1,ENST00000305510,MSTRG.18891.2	MSTRG.18891.3	9	+	2	96817503	96825057	96825058	96825201	96828996	96829134	96829135	96832551	3	8	6	7554	143	138	3416	140.5	5485	834	1
ENSG00000112877:+:5:647804:655584	ENSG00000112877	MSTRG.25859.4,MSTRG.25859.9,MSTRG.25859.2,MSTRG.25859.15,MSTRG.25859.14,MSTRG.25859.5,MSTRG.25859.16,MSTRG.25859.8,MSTRG.25859.1,MSTRG.25859.6,MSTRG.25859.7,MSTRG.25859.11,MSTRG.25859.13,MSTRG.25859.12	MSTRG.25859.4	16	+	5	644426	647804	647805	647916	655456	655584	655585	663415	11	13	3	3378	111	128	7830	119.5	5604	445	1
ENSG00000067225:-:15:72206727:72206880	ENSG00000067225	MSTRG.11164.7,MSTRG.11164.1,ENST00000319622,ENST00000565184,ENST00000335181,ENST00000567118,ENST00000389093,MSTRG.11164.10,ENST00000565154,ENST00000568459,ENST00000561609,ENST00000569857,ENST00000563986,ENST00000570166	MSTRG.11164.7	11	-	15	72207126	72206881	72206880	72206728	72206880	72206728	72206727	72203189	8	8	1	245	152	152	3538	152	1891.5	153	1
ENSG00000133612:+:7:151116792:151134568	ENSG00000133612	MSTRG.30937.6,MSTRG.30937.7,ENST00000397238,MSTRG.30937.10,ENST00000622464,MSTRG.30937.19,MSTRG.30937.3,MSTRG.30937.14,MSTRG.30937.8,MSTRG.30937.9,ENST00000463381,MSTRG.30937.20	MSTRG.30937.6	17	+	7	151087073	151116792	151116793	151116851	151134400	151134568	151134569	151141897	2	12	11	29719	58	168	7328	113	18523.5	2459	1
ENSG00000112699:-:6:1930102:1960966	ENSG00000112699	ENST00000380815,ENST00000530927,ENST00000531690,ENST00000530459	ENST00000380815	11	-	6	2115770	1960967	1960966	1960774	1930230	1930103	1930102	1742587	5	7	3	154803	192	127	187515	159.5	171159	426	1
ENSG00000100139:+:22:37912350:37932888	ENSG00000100139	MSTRG.22088.4,ENST00000215957,MSTRG.22088.2,MSTRG.22088.13,ENST00000680578,ENST00000424008,ENST00000680501,ENST00000445494,ENST00000454685,ENST00000681920	MSTRG.22088.4	18	+	22	37912001	37912350	37912351	37912492	37932798	37932888	37932889	37933038	4	13	10	349	141	90	149	115.5	249	2039	1
ENSG00000105835:-:7:106263391:106269312	ENSG00000105835	ENST00000680991,ENST00000489732,ENST00000424768,ENST00000681494,ENST00000680708,ENST00000681391,ENST00000681255,ENST00000681456,ENST00000680077,ENST00000681653,ENST00000222553,ENST00000680584,ENST00000680864,ENST00000681550,ENST00000681491,ENST00000679873,ENST00000680468,ENST00000680675,ENST00000679461,ENST00000679643,ENST00000679717,ENST00000681878,ENST00000680823,ENST00000679951,ENST00000681936,ENST00000679894,ENST00000681223,ENST00000680129,ENST00000680786,ENST00000681887,ENST00000354289,ENST00000484527	ENST00000680991	11	-	7	106272529	106269313	106269312	106269154	106263617	106263392	106263391	106261708	5	7	3	3216	158	225	1683	191.5	2449.5	522	1
ENSG00000131149:+:16:85633913:85634132	ENSG00000131149	MSTRG.13159.1,MSTRG.13159.7,ENST00000253458,MSTRG.13159.11,MSTRG.13159.9,MSTRG.13159.18,ENST00000637419	MSTRG.13159.1	17	+	16	85357644	85633913	85633914	85634132	85633914	85634132	85634133	85648551	3	3	1	276269	218	218	14418	218	145343.5	219	1
ENSG00000112118:-:6:52269085:52279599	ENSG00000112118	ENST00000616552,ENST00000596288,ENST00000229854,MSTRG.28402.4,MSTRG.28402.7,MSTRG.28402.6,MSTRG.28402.20,MSTRG.28402.21,MSTRG.28402.8,MSTRG.28402.10,MSTRG.28402.16,MSTRG.28402.14,ENST00000419835,MSTRG.28402.13,MSTRG.28402.22,MSTRG.28402.18	ENST00000616552	17	-	6	52282044	52279600	52279599	52279361	52269226	52269086	52269085	52267969	5	13	9	2444	238	140	1116	189	1780	1437	1
ENSG00000171456:+:20:32366383:32369123	ENSG00000171456	ENST00000375687,ENST00000613218,ENST00000646985,ENST00000306058,MSTRG.20555.1,ENST00000620121,MSTRG.20555.12,MSTRG.20555.10,ENST00000651418,MSTRG.20555.11,MSTRG.20555.13,MSTRG.20555.20,MSTRG.20555.9,MSTRG.20555.18,ENST00000649901,ENST00000555343,ENST00000470145,ENST00000644587,ENST00000646367,ENST00000645337,ENST00000645035,ENST00000643168,ENST00000642676,ENST00000375689,ENST00000497249,ENST00000645688	ENST00000375687	13	+	20	32358833	32366383	32366384	32366466	32369015	32369123	32369124	32428127	2	4	3	7550	82	108	59003	95	33276.5	195	1
ENSG00000137073:-:9:33941648:33953474	ENSG00000137073	ENST00000684298,ENST00000682209,ENST00000683252,ENST00000682239,ENST00000684158,MSTRG.32567.21,ENST00000379238,ENST00000683084,ENST00000681980,ENST00000683659,ENST00000683140,ENST00000360802,MSTRG.32567.14,ENST00000379239,MSTRG.32567.22,MSTRG.32567.24,ENST00000684754,MSTRG.32567.20,MSTRG.32567.26,MSTRG.32567.25,ENST00000629575,ENST00000683717,ENST00000412543	ENST00000684298	15	-	9	33960825	33953475	33953474	33953285	33941862	33941649	33941648	33935879	10	14	5	7350	189	213	5769	201	6559.5	1063	1
ENSG00000183527:-:21:39177434:39181878	ENSG00000183527	ENST00000331573,ENST00000411828,MSTRG.21356.3,MSTRG.21356.7,MSTRG.21356.2,ENST00000380900	ENST00000331573	7	-	21	39183251	39181879	39181878	39181772	39177571	39177435	39177434	39175665	2	6	5	1372	106	136	1769	121	1570.5	658	1
ENSG00000144935:+:3:142736378:142748460	ENSG00000144935	ENST00000476941,MSTRG.23851.2,ENST00000273482,MSTRG.23851.6	ENST00000476941	13	+	3	142724732	142736378	142736379	142736533	142748258	142748460	142748461	142777631	2	4	3	11646	154	202	29170	178	20408	460	1
ENSG00000100403:+:22:41320654:41332227	ENSG00000100403	ENST00000352645,MSTRG.22204.3,MSTRG.22204.9,MSTRG.22204.7	ENST00000352645	23	+	22	41301773	41320654	41320655	41320713	41332171	41332227	41332228	41338312	2	7	6	18881	58	56	6084	57	12482.5	588	1
ENSG00000171634:+:17:67975771:67975958	ENSG00000171634	ENST00000321892,ENST00000644067,ENST00000306378,MSTRG.14973.6,ENST00000342579,ENST00000424123,MSTRG.14973.10,MSTRG.14973.12,MSTRG.14973.17,MSTRG.14973.7,MSTRG.14973.2,MSTRG.14973.19,MSTRG.14973.13,MSTRG.14973.5,MSTRG.14973.11,MSTRG.14973.14,MSTRG.14973.16,MSTRG.14973.15,ENST00000578307,MSTRG.14973.27,MSTRG.14973.24,MSTRG.14973.30,ENST00000581258	ENST00000321892	30	+	17	67966657	67975771	67975772	67975958	67975772	67975958	67975959	67982251	29	29	1	9114	186	186	6292	186	7703	187	1
ENSG00000148516:+:10:31355143:31387798	ENSG00000148516	ENST00000493835	ENST00000493835	6	+	10			31355144	31355192	31387720	31387798	31387799	31389604	1	4	4		48	78	1805	63	1805	427	1
ENSG00000109674:+:4:177322458:177353728	ENSG00000109674	ENST00000264596,MSTRG.25756.3,MSTRG.25756.2	ENST00000264596	10	+	4	177310110	177322458	177322459	177322580	177353308	177353728	177353729	177360502	2	8	7	12348	121	420	6773	270.5	9560.5	1304	1
ENSG00000273416_ENSG00000173726:-:1:235119817:235122372	ENSG00000273416_ENSG00000173726	MSTRG.3310.1	MSTRG.3310.1	5	-	1	235128594	235122373	235122372	235122326	235119899	235119818	235119817	235113911	2	3	2	6221	46	81	5906	63.5	6063.5	129	1
ENSG00000188352:+:9:20862577:20867012	ENSG00000188352	ENST00000380249,MSTRG.32470.1,MSTRG.32470.4,ENST00000338382,ENST00000605086,MSTRG.32470.5	ENST00000380249	46	+	9	20823116	20862577	20862578	20862712	20866929	20867012	20867013	20874680	18	20	3	39461	134	83	7667	108.5	23564	270	1
ENSG00000276418:-:8:80002998:80042668	ENSG00000276418	ENST00000522938,MSTRG.31712.6,MSTRG.31712.3	ENST00000522938	8	-	8	80051526	80042669	80042668	80042620	80003180	80002999	80002998	79958436	5	6	2	8857	48	181	44562	114.5	26709.5	231	1
ENSG00000157450:+:15:59052304:59058550	ENSG00000157450	ENST00000348370,ENST00000557998,ENST00000559209,ENST00000561186,MSTRG.10928.3,MSTRG.10928.4,MSTRG.10928.11,MSTRG.10928.10	ENST00000348370	14	+	15	59031703	59052304	59052305	59052431	59058356	59058550	59058551	59066763	3	5	3	20601	126	194	8212	160	14406.5	486	1
ENSG00000158417:+:2:99371661:99371730	ENSG00000158417	MSTRG.18937.2,ENST00000617677	MSTRG.18937.2	24	+	2	99369486	99371661	99371662	99371730	99371662	99371730	99371731	99376346	9	9	1	2175	68	68	4615	68	3395	69	1
ENSG00000285733:-:6:169582835:169689012	ENSG00000285733	MSTRG.29337.6,ENST00000648086	MSTRG.29337.6	19	-	6	169689126	169689013	169689012	169688817	169582934	169582836	169582835	169433403	3	17	15	113	195	98	149432	146.5	74772.5	1643	1
ENSG00000155561:+:7:135577795:135593192	ENSG00000155561	MSTRG.30755.2,MSTRG.30755.5,MSTRG.30755.6,MSTRG.30755.9,MSTRG.30755.3,MSTRG.30755.1,MSTRG.30755.4,MSTRG.30755.10,ENST00000285968,MSTRG.30755.7,MSTRG.30755.8,MSTRG.30755.11	MSTRG.30755.2	43	+	7	135577129	135577795	135577796	135578024	135592987	135593192	135593193	135594546	6	12	7	666	228	205	1353	216.5	1009.5	1193	1
ENSG00000163738:+:4:74174505:74201370	ENSG00000163738	MSTRG.25035.10,ENST00000359107,ENST00000395759,ENST00000325278,ENST00000429519,ENST00000433372,MSTRG.25035.8,MSTRG.25035.14,MSTRG.25035.9	MSTRG.25035.10	9	+	4	74158282	74174505	74174506	74174690	74201263	74201370	74201371	74225301	2	6	5	16223	184	107	23930	145.5	20076.5	669	1
ENSG00000172943:-:X:53984913:54002682	ENSG00000172943	ENST00000686349,ENST00000687764,ENST00000338154,MSTRG.34303.4,MSTRG.34303.3,ENST00000357988,ENST00000338946,MSTRG.34303.12,MSTRG.34303.13,MSTRG.34303.10,ENST00000691629,MSTRG.34303.15,MSTRG.34303.14,MSTRG.34303.11,MSTRG.34303.17,ENST00000396282,ENST00000322659,ENST00000443302	ENST00000686349	21	-	X	54011121	54002683	54002682	54002595	53985949	53984914	53984913	53962940	9	17	9	8438	87	1035	21973	561	15205.5	2085	1
ENSG00000037637:-:1:16305802:16305919	ENSG00000037637	ENST00000375592,ENST00000478089,MSTRG.396.2,MSTRG.396.3,MSTRG.396.11,MSTRG.396.9,MSTRG.396.8	ENST00000375592	10	-	1	16315168	16305920	16305919	16305803	16305919	16305803	16305802	16294918	3	3	1	9248	116	116	10884	116	10066	117	1
ENSG00000132155:-:3:12618514:12618747	ENSG00000132155	ENST00000686409,ENST00000687326,ENST00000686455,ENST00000692558,ENST00000687257,ENST00000689540,ENST00000688444,ENST00000689418,ENST00000688625,ENST00000685348,ENST00000688543,ENST00000685653,ENST00000688803,ENST00000690625,ENST00000692311,ENST00000688269,ENST00000685738,ENST00000442415,ENST00000689876,ENST00000689914,ENST00000251849,ENST00000692093,ENST00000691724,ENST00000686762,MSTRG.22508.42,ENST00000690460,ENST00000689097,ENST00000693664,ENST00000692773,ENST00000691396,ENST00000423275,ENST00000684903,ENST00000687923,ENST00000690397,ENST00000691779,ENST00000691899,ENST00000689389,ENST00000685437,ENST00000693705,ENST00000692830,ENST00000687940,ENST00000689481,ENST00000686479,ENST00000491290,ENST00000692777,ENST00000692959,ENST00000693069,ENST00000691718,ENST00000689226,ENST00000685740,ENST00000689033,ENST00000685959,ENST00000416093,ENST00000687348,ENST00000688753	ENST00000686409	11	-	3	12663812	12618748	12618747	12618515	12618747	12618515	12618514	12612063	2	2	1	45064	232	232	6451	232	25757.5	233	1
ENSG00000169519:+:11:28211061:28290397	ENSG00000169519	ENST00000406787,ENST00000407364,ENST00000303459,ENST00000451385,MSTRG.5321.6,MSTRG.5321.7,MSTRG.5321.4,MSTRG.5321.9,ENST00000634973,ENST00000634627,MSTRG.5321.12,MSTRG.5321.11	ENST00000406787	7	+	11	28113605	28211061	28211062	28211198	28290206	28290397	28290398	28296796	4	5	2	97456	136	191	6398	163.5	51927	329	1
ENSG00000183098:+:13:93545262:93545421	ENSG00000183098	ENST00000377047	ENST00000377047	9	+	13	93227617	93545262	93545263	93545421	93545263	93545421	93545422	93830153	2	2	1	317645	158	158	284731	158	301188	159	1
ENSG00000061676:-:2:182934751:182942163	ENSG00000061676	ENST00000361354,ENST00000360982,ENST00000478449,ENST00000471640	ENST00000361354	31	-	2	182952404	182942164	182942163	182942070	182934832	182934752	182934751	182930789	24	26	3	10240	93	80	3962	86.5	7101	258	1
ENSG00000187079:+:11:12764178:12764434	ENSG00000187079	ENST00000527636,MSTRG.5183.3,MSTRG.5183.2,MSTRG.5183.6,MSTRG.5183.8,ENST00000334310,ENST00000527575,MSTRG.5183.7,MSTRG.5183.5,ENST00000527376	ENST00000527636	13	+	11	12675562	12764178	12764179	12764434	12764179	12764434	12764435	12862249	3	3	1	88616	255	255	97814	255	93215	256	1
ENSG00000057663:-:6:106293027:106293106	ENSG00000057663	ENST00000369076,ENST00000343245,ENST00000635758,ENST00000636437,ENST00000646025,ENST00000636335,MSTRG.28708.9,ENST00000613993	ENST00000369076	8	-	6	106308363	106293107	106293106	106293028	106293106	106293028	106293027	106279824	4	4	1	15256	78	78	13203	78	14229.5	79	1
ENSG00000170315:+:17:16381901:16382130	ENSG00000170315	ENST00000535788,ENST00000577958	ENST00000535788	3	+	17	16381185	16381901	16381902	16382130	16381902	16382130	16382131	16382358	2	2	1	716	228	228	227	228	471.5	229	1
ENSG00000022277:+:20:56473300:56484189	ENSG00000022277	ENST00000023939,ENST00000357348,ENST00000395881,MSTRG.20883.7,MSTRG.20883.5,MSTRG.20883.3,ENST00000449062	ENST00000023939	10	+	20	56472416	56473300	56473301	56473395	56484111	56484189	56484190	56513314	3	6	4	884	94	78	29124	86	15004	408	1
ENSG00000104714:-:8:668597:674047	ENSG00000104714	ENST00000262109,ENST00000522706,MSTRG.31028.6	ENST00000262109	6	-	8	692477	674048	674047	673289	668792	668598	668597	664677	4	5	2	18429	758	194	3920	476	11174.5	954	1
ENSG00000167081:+:9:125929654:125935607	ENSG00000167081	ENST00000672674,ENST00000373487,ENST00000373489,ENST00000447726,ENST00000342287,ENST00000373482,ENST00000538998,ENST00000373492,ENST00000673571,ENST00000428092	ENST00000672674	10	+	9	125915928	125929654	125929655	125929845	125935472	125935607	125935608	125960683	6	7	2	13726	190	135	25075	162.5	19400.5	327	1
ENSG00000111358:+:12:123639263:123652735	ENSG00000111358	MSTRG.8256.3,MSTRG.8256.1,ENST00000543341,ENST00000543415,ENST00000618160,ENST00000536375,ENST00000538845,ENST00000538533,ENST00000537695,ENST00000542279	MSTRG.8256.3	13	+	12	123633873	123639263	123639264	123639343	123652707	123652735	123652736	123654923	2	7	6	5390	79	28	2187	53.5	3788.5	473	1
ENSG00000155313:+:21:15808808:15849872	ENSG00000155313	ENST00000400183,ENST00000285681	ENST00000400183	26	+	21	15805259	15808808	15808809	15808885	15849777	15849872	15849873	15864267	8	20	13	3549	76	95	14394	85.5	8971.5	1767	1
ENSG00000117114:+:1:81836884:81907230	ENSG00000117114	ENST00000674492,ENST00000674373,ENST00000370725,ENST00000370723,ENST00000370728,ENST00000370727,ENST00000370730,ENST00000370721,MSTRG.1489.45,MSTRG.1489.47,ENST00000674307,ENST00000686636,MSTRG.1489.46,ENST00000674407,MSTRG.1489.49,ENST00000370717,ENST00000674209,ENST00000370715,ENST00000627151,MSTRG.1489.38,MSTRG.1489.35,MSTRG.1489.31,ENST00000674442,ENST00000674168,ENST00000674419,ENST00000674208,ENST00000674393,MSTRG.1489.48,MSTRG.1489.43,MSTRG.1489.26,MSTRG.1489.37,ENST00000319517,MSTRG.1489.32,ENST00000359929,MSTRG.1489.52,MSTRG.1489.34,ENST00000370713,MSTRG.1489.51,MSTRG.1489.50,MSTRG.1489.41,MSTRG.1489.33,ENST00000674489,ENST00000469377,ENST00000674367,ENST00000674410,ENST00000674333,MSTRG.1489.44	ENST00000674492	24	+	1	81800480	81836884	81836885	81837057	81907017	81907230	81907231	81936727	2	3	2	36404	172	213	29496	192.5	32950	387	1
ENSG00000122566:-:7:26193250:26193373	ENSG00000122566	ENST00000463181,ENST00000490912,ENST00000677075,ENST00000678075,ENST00000678035,ENST00000356674,ENST00000677839,ENST00000354667,ENST00000618183,ENST00000679021,ENST00000677906,ENST00000677669,ENST00000679123,ENST00000677574,ENST00000678277,ENST00000679124,ENST00000678183,ENST00000677571,ENST00000678973,ENST00000677321,ENST00000678962,ENST00000678501,ENST00000679001,ENST00000678449,ENST00000677339,ENST00000608362,ENST00000679243,ENST00000678431,ENST00000677656,ENST00000676903,ENST00000677631,ENST00000678675,ENST00000676749,ENST00000676524,ENST00000676497,MSTRG.29609.62,ENST00000676746,ENST00000678935,ENST00000678998,ENST00000677396,ENST00000678697,MSTRG.29609.8,ENST00000678779,MSTRG.29609.65,MSTRG.29609.30,ENST00000360787,MSTRG.29609.70,ENST00000679318,MSTRG.29609.51,ENST00000677037,ENST00000678884,MSTRG.29609.67,MSTRG.29609.50,MSTRG.29609.66,MSTRG.29609.72,MSTRG.29609.69,MSTRG.29609.52,MSTRG.29609.53,MSTRG.29609.49,MSTRG.29609.74,MSTRG.29609.71,MSTRG.29609.55,MSTRG.29609.11,MSTRG.29609.57,MSTRG.29609.60,MSTRG.29609.59,MSTRG.29609.63,MSTRG.29609.68,MSTRG.29609.9,MSTRG.29609.10	ENST00000463181	9	-	7	26193574	26193374	26193373	26193251	26193373	26193251	26193250	26192578	8	8	1	200	122	122	672	122	436	123	1
ENSG00000143207:-:1:175986942:176085890	ENSG00000143207	ENST00000649803,ENST00000367669,MSTRG.2642.7,MSTRG.2642.4,ENST00000367667,MSTRG.2642.9,ENST00000308769,ENST00000367666	ENST00000649803	21	-	1	176116623	176085891	176085890	176085776	175987103	175986943	175986942	175947240	11	19	9	30732	114	160	39702	137	35217	1107	1
ENSG00000144741:+:3:66262050:66346408	ENSG00000144741	MSTRG.23213.14,MSTRG.23213.5,ENST00000691461,ENST00000464350,ENST00000686511,ENST00000690560,ENST00000688696,ENST00000691582,ENST00000354883,ENST00000690634,ENST00000691525,ENST00000483224,MSTRG.23213.1,MSTRG.23213.8,MSTRG.23213.4,MSTRG.23213.20,ENST00000676754,MSTRG.23213.3,ENST00000413054,ENST00000336733,ENST00000484768	MSTRG.23213.14	11	+	3	66243313	66262050	66262051	66262155	66346364	66346408	66346409	66362859	4	6	3	18737	104	44	16450	74	17593.5	198	1
ENSG00000139746:-:13:79365577:79366195	ENSG00000139746	MSTRG.8946.3,MSTRG.8946.4,MSTRG.8946.7,MSTRG.8946.8,MSTRG.8946.11,MSTRG.8946.10,MSTRG.8946.5,MSTRG.8946.12,MSTRG.8946.14,MSTRG.8946.17,ENST00000622611,ENST00000438737,ENST00000438724,MSTRG.8946.22,MSTRG.8946.9,MSTRG.8946.30,MSTRG.8946.27,MSTRG.8946.26,ENST00000267229,MSTRG.8946.25,MSTRG.8946.24	MSTRG.8946.3	23	-	13	79366632	79366196	79366195	79366055	79365718	79365578	79365577	79359687	8	9	2	436	140	140	5890	140	3163	282	1
ENSG00000144935:+:3:142748257:142781029	ENSG00000144935	ENST00000476941,MSTRG.23851.2,ENST00000273482,MSTRG.23851.6,MSTRG.23851.5,MSTRG.23851.4	ENST00000476941	13	+	3	142743587	142748257	142748258	142748460	142780834	142781029	142781030	142784703	4	6	3	4670	202	195	3673	198.5	4171.5	531	1
ENSG00000148356:+:9:127479382:127479505	ENSG00000148356	ENST00000676318,ENST00000674516,ENST00000674970,ENST00000498513,ENST00000323301,ENST00000676349,ENST00000675141,ENST00000676170,ENST00000675883,ENST00000675224,ENST00000675253,ENST00000675641,ENST00000300417,ENST00000675657,ENST00000675945,ENST00000675448,ENST00000373324,ENST00000675445,ENST00000674771,ENST00000675572,ENST00000676336,ENST00000676014,ENST00000674784,ENST00000675789,ENST00000675213,ENST00000373322,ENST00000676409,ENST00000676106,ENST00000676137,ENST00000675012,ENST00000675521,ENST00000676399,ENST00000675198,ENST00000675662,ENST00000472068,ENST00000676035,ENST00000674511,ENST00000674621	ENST00000676318	25	+	9	127478964	127479382	127479383	127479505	127479383	127479505	127479506	127479838	12	12	1	418	122	122	332	122	375	123	1
ENSG00000111885:-:6:119290682:119306992	ENSG00000111885	ENST00000368468	ENST00000368468	13	-	6	119348462	119306993	119306992	119306896	119290763	119290683	119290682	119248355	3	5	3	41469	96	80	42327	88	41898	294	1
ENSG00000103227:-:16:954356:954666	ENSG00000103227	ENST00000545827,ENST00000262301,ENST00000568897,ENST00000570014,ENST00000562226,ENST00000568964,ENST00000567595,ENST00000566609,ENST00000562380	ENST00000545827	12	-	16	981144	954667	954666	954357	954666	954357	954356	934255	2	2	1	26477	309	309	20101	309	23289	310	1
ENSG00000066735:+:14:104152014:104167048	ENSG00000066735	MSTRG.10248.2,ENST00000423312,MSTRG.10248.5,MSTRG.10248.1,MSTRG.10248.4,ENST00000315264	MSTRG.10248.2	15	+	14	104139289	104152014	104152015	104152461	104166859	104167048	104167049	104171722	3	5	3	12725	446	189	4673	317.5	8699	825	1
ENSG00000197157:+:7:127686612:127807573	ENSG00000197157	ENST00000354725,MSTRG.30622.1,MSTRG.30622.5,MSTRG.30622.3,MSTRG.30622.7,MSTRG.30622.2,MSTRG.30622.4	ENST00000354725	24	+	7	127652452	127686612	127686613	127686762	127807484	127807573	127807574	127844323	2	11	10	34160	149	89	36749	119	35454.5	1164	1
ENSG00000177885:-:17:75393550:75393765	ENSG00000177885	ENST00000392562,ENST00000316804,ENST00000316615,ENST00000392563,MSTRG.15060.9,MSTRG.15060.8,ENST00000648046,MSTRG.15060.10,MSTRG.15060.1,ENST00000582582	ENST00000392562	6	-	17	75404840	75393766	75393765	75393551	75393765	75393551	75393550	75332798	2	2	1	11074	214	214	60752	214	35913	215	1
ENSG00000272325:-:6:34341861:34341972	ENSG00000272325	ENST00000607016	ENST00000607016	5	-	6	34392263	34341973	34341972	34341862	34341972	34341862	34341861	34295686	2	2	1	50290	110	110	46175	110	48232.5	111	1
ENSG00000146143:+:6:57379900:57382168	ENSG00000146143	ENST00000672107,ENST00000615550	ENST00000672107	16	+	6	57326046	57379900	57379901	57379996	57382031	57382168	57382169	57398004	6	7	2	53854	95	137	15835	116	34844.5	234	1
ENSG00000134871:+:13:110357471:110357552	ENSG00000134871	ENST00000360467,ENST00000650540,ENST00000400163	ENST00000360467	48	+	13	110308124	110357471	110357472	110357552	110357472	110357552	110357553	110424733	4	4	1	49347	80	80	67180	80	58263.5	81	1
ENSG00000139746:-:13:79318820:79320710	ENSG00000139746	MSTRG.8946.3,MSTRG.8946.4,MSTRG.8946.7,MSTRG.8946.6,MSTRG.8946.8,MSTRG.8946.11,MSTRG.8946.5,MSTRG.8946.12,MSTRG.8946.14,MSTRG.8946.19,MSTRG.8946.32,MSTRG.8946.22,MSTRG.8946.30,MSTRG.8946.29,MSTRG.8946.26,MSTRG.8946.31,MSTRG.8946.15,MSTRG.8946.25	MSTRG.8946.3	23	-	13	79322348	79320711	79320710	79319535	79318879	79318821	79318820	79314994	21	22	2	1637	1175	58	3826	616.5	2731.5	1235	1
ENSG00000186298:-:12:110730528:110731901	ENSG00000186298	ENST00000335007,ENST00000550991,MSTRG.8013.7,ENST00000551676,MSTRG.8013.1,MSTRG.8013.9,ENST00000340766,MSTRG.8013.2,MSTRG.8013.12,ENST00000620030	ENST00000335007	7	-	12	110742652	110731902	110731901	110731770	110730759	110730529	110730528	110724765	2	3	2	10750	131	230	5763	180.5	8256.5	363	1
ENSG00000204217:+:2:202464808:202467689	ENSG00000204217	ENST00000374580,ENST00000374574	ENST00000374580	13	+	2	202377551	202464808	202464809	202464979	202467519	202467689	202467690	202513718	2	3	2	87257	170	170	46028	170	66642.5	342	1
ENSG00000124795:-:6:18255730:18256455	ENSG00000124795	ENST00000652689,ENST00000507591,ENST00000515770,ENST00000651992,MSTRG.27700.2,MSTRG.27700.5,MSTRG.27700.8,MSTRG.27700.11,MSTRG.27700.12,ENST00000244776,ENST00000505224,ENST00000652576,ENST00000512145,ENST00000503715	ENST00000652689	11	-	6	18257952	18256456	18256455	18256361	18255851	18255731	18255730	18249840	5	6	2	1496	94	120	5890	107	3693	216	1
ENSG00000083720:-:5:41840450:41862750	ENSG00000083720	ENST00000196371	ENST00000196371	17	-	5	41870280	41862751	41862750	41862642	41840511	41840451	41840450	41807439	2	7	6	7529	108	60	33011	84	20270	654	1
ENSG00000044115:+:5:138781922:138783372	ENSG00000044115	ENST00000627109,ENST00000302763,ENST00000518825,MSTRG.27006.5,MSTRG.27006.11,ENST00000519309,ENST00000519113,ENST00000520339,ENST00000517980,ENST00000520158,ENST00000522227,ENST00000523912,ENST00000524127	ENST00000627109	19	+	5	138753511	138781922	138781923	138782029	138783177	138783372	138783373	138810037	2	3	2	28411	106	195	26664	150.5	27537.5	303	1
ENSG00000066135:+:1:43655590:43689095	ENSG00000066135	MSTRG.1029.1,MSTRG.1029.4,ENST00000372396,MSTRG.1029.5	MSTRG.1029.1	23	+	1	43653800	43655590	43655591	43655766	43688914	43689095	43689096	43690844	4	14	11	1790	175	181	1748	178	1769	1899	1
ENSG00000185104:-:1:50535368:50596216	ENSG00000185104	ENST00000396153,MSTRG.1146.2,MSTRG.1146.3,MSTRG.1146.4,ENST00000494400	ENST00000396153	19	-	1	50655441	50596217	50596216	50596121	50535457	50535369	50535368	50491802	9	15	7	59224	95	88	43566	91.5	51395	750	1
ENSG00000198162:+:1:117402185:117420649	ENSG00000198162	ENST00000356554,MSTRG.1897.3,MSTRG.1897.5,MSTRG.1897.2	ENST00000356554	13	+	1	117368486	117402185	117402186	117402441	117420569	117420649	117420650	117442230	2	5	4	33699	255	80	21580	167.5	27639.5	553	1
ENSG00000198589:-:4:150415437:150435708	ENSG00000198589	ENST00000357115,ENST00000651943,MSTRG.25594.4,ENST00000510413,MSTRG.25594.7,MSTRG.25594.11,ENST00000651035,MSTRG.25594.15,ENST00000507224,ENST00000651695,ENST00000509835,ENST00000503716,ENST00000648823,ENST00000648878,ENST00000649874	ENST00000357115	58	-	4	150436723	150435709	150435708	150435589	150415590	150415438	150415437	150350160	47	48	2	1014	119	152	65277	135.5	33145.5	273	1
ENSG00000109762:+:4:185247293:185310816	ENSG00000109762	ENST00000652585,ENST00000504273,ENST00000264694,ENST00000618785,MSTRG.25817.4,MSTRG.25817.5	ENST00000652585	19	+	4	185210256	185247293	185247294	185247378	185310635	185310816	185310817	185320732	2	7	6	37037	84	181	9915	132.5	23476	915	1
ENSG00000138430:-:2:174223032:174229451	ENSG00000138430	ENST00000284719,ENST00000409546,ENST00000344357,MSTRG.19514.2,ENST00000428402	ENST00000284719	11	-	2	174246714	174229452	174229451	174229308	174223160	174223033	174223032	174142001	3	4	2	17262	143	127	81031	135	49146.5	272	1
ENSG00000130816:-:19:10166597:10168364	ENSG00000130816	ENST00000676610,ENST00000676820,ENST00000678024,ENST00000678804,ENST00000677946,MSTRG.16395.4,ENST00000340748,ENST00000359526,ENST00000592705,MSTRG.16395.2,MSTRG.16395.8,ENST00000677013,ENST00000677634,ENST00000679103,ENST00000679313,ENST00000677616,ENST00000677685,ENST00000676604	ENST00000676610	38	-	19	10173089	10168365	10168364	10168330	10166685	10166598	10166597	10163361	9	10	2	4724	34	87	3236	60.5	3980	123	1
ENSG00000136877:+:9:127813194:127813323	ENSG00000136877	MSTRG.33427.5,MSTRG.33427.6,ENST00000467826,ENST00000488506	MSTRG.33427.5	16	+	9	127811012	127813194	127813195	127813323	127813195	127813323	127813324	127814207	15	15	1	2182	128	128	883	128	1532.5	129	1
ENSG00000068383:+:10:132726820:132765846	ENSG00000068383	ENST00000368594,MSTRG.4833.3,MSTRG.4833.4,ENST00000368593,ENST00000342652	ENST00000368594	16	+	10	132710457	132726820	132726821	132726905	132765773	132765846	132765847	132777670	9	12	4	16363	84	73	11823	78.5	14093	330	1
ENSG00000215158:-:5:34179012:34182867	ENSG00000215158	ENST00000514048,ENST00000507274	ENST00000514048	6	-	5	34189471	34182868	34182867	34182680	34179097	34179013	34179012	34175958	2	3	2	6603	187	84	3054	135.5	4828.5	273	1
ENSG00000146263:-:6:97246627:97254733	ENSG00000146263	ENST00000275053,ENST00000683635,ENST00000369251,MSTRG.28676.4,ENST00000509383,ENST00000510018,ENST00000482634	ENST00000275053	25	-	6	97263334	97254734	97254733	97254557	97246690	97246628	97246627	97233981	10	11	2	8600	176	62	12646	119	10623	240	1
ENSG00000229807:-:X:73829067:73833374	ENSG00000229807	ENST00000429829,ENST00000650627,ENST00000650366,ENST00000650186,ENST00000648970,ENST00000647913,ENST00000647696,MSTRG.34462.23,ENST00000669898,ENST00000648927,ENST00000421322,ENST00000650637,ENST00000666309,MSTRG.34462.12,MSTRG.34462.1,MSTRG.34462.5,ENST00000665247,ENST00000635841,ENST00000434839,MSTRG.34462.33,MSTRG.34462.28,MSTRG.34462.30	ENST00000429829	6	-	X	73837439	73833375	73833374	73833238	73829231	73829068	73829067	73827985	3	5	3	4064	136	163	1082	149.5	2573	510	1
ENSG00000141556:+:17:82805874:82900731	ENSG00000141556	ENST00000681983,ENST00000684361,ENST00000684760,ENST00000683444,ENST00000682479,ENST00000684464,ENST00000684429,ENST00000539345,ENST00000355528,ENST00000682722,ENST00000684000,ENST00000683041,ENST00000683282,ENST00000683184,ENST00000684188,ENST00000682213,ENST00000682610,ENST00000683009	ENST00000681983	38	+	17	82800997	82805874	82805875	82806011	82900651	82900731	82900732	82903404	10	18	9	4877	136	80	2672	108	3774.5	780	1
ENSG00000060237:+:12:813641:830160	ENSG00000060237	ENST00000530271,ENST00000340908,ENST00000537687,ENST00000315939,ENST00000535572,MSTRG.6611.10,MSTRG.6611.9,MSTRG.6611.8,MSTRG.6611.12,MSTRG.6611.6,MSTRG.6611.15,MSTRG.6611.14,MSTRG.6611.13,MSTRG.6611.11,MSTRG.6611.16,MSTRG.6611.17	ENST00000530271	31	+	12	754325	813641	813642	813814	830003	830160	830161	857160	2	4	3	59316	172	157	26999	164.5	43157.5	552	1
ENSG00000138768:+:4:75774675:75812375	ENSG00000138768	ENST00000264904,ENST00000514213,MSTRG.25047.2	ENST00000264904	26	+	4	75771138	75774675	75774676	75774796	75812160	75812375	75812376	75813205	9	25	17	3537	120	215	829	167.5	2183	2265	1
ENSG00000151466:-:4:128888678:128999794	ENSG00000151466	ENST00000651532,ENST00000281142,MSTRG.25461.2,ENST00000503215	ENST00000651532	23	-	4	129003740	128999795	128999794	128999672	128888774	128888679	128888678	128874467	7	19	13	3945	122	95	14211	108.5	9078	1385	1
ENSG00000049618:+:6:156829226:156935576	ENSG00000049618	ENST00000346085,ENST00000636930,MSTRG.29225.3,ENST00000647938,ENST00000350026,MSTRG.29225.12,MSTRG.29225.10,MSTRG.29225.15,ENST00000414678,MSTRG.29225.11,MSTRG.29225.13,MSTRG.29225.20,MSTRG.29225.14,MSTRG.29225.9,MSTRG.29225.4,ENST00000674298,ENST00000674190,ENST00000638000	ENST00000346085	21	+	6	156779472	156829226	156829227	156829421	156935466	156935576	156935577	157084661	3	6	4	49754	194	110	149084	152	99419	495	1
ENSG00000198879:-:10:7276891:7285954	ENSG00000198879	ENST00000397167,ENST00000361972,ENST00000673876,ENST00000683762,MSTRG.3508.6,ENST00000682896	ENST00000397167	21	-	10	7367648	7285955	7285954	7285866	7276989	7276892	7276891	7248650	5	7	3	81693	88	97	28241	92.5	54967	434	1
ENSG00000171793:+:1:40983277:40984991	ENSG00000171793	ENST00000649864,ENST00000650634,ENST00000648801,ENST00000648020,ENST00000696107,ENST00000470271,ENST00000696109,ENST00000649124,ENST00000696108,ENST00000498694,ENST00000650070,ENST00000480420,ENST00000648914,MSTRG.977.18,ENST00000479480,MSTRG.977.13,ENST00000463285	ENST00000649864	17	+	1	40979830	40983277	40983278	40983456	40984821	40984991	40984992	40987371	2	3	2	3447	178	170	2379	174	2913	350	1
ENSG00000019995:+:10:124942456:124943307	ENSG00000019995	MSTRG.4787.1,MSTRG.4787.2,MSTRG.4787.5,MSTRG.4787.8,MSTRG.4787.9,MSTRG.4787.4	MSTRG.4787.1	10	+	10	124917273	124942456	124942457	124943307	124942457	124943307	124943308	124966593	2	2	1	25183	850	850	23285	850	24234	851	1
ENSG00000126934:-:19:4117418:4117629	ENSG00000126934	MSTRG.16197.5,ENST00000262948,MSTRG.16197.13,MSTRG.16197.12,MSTRG.16197.2,MSTRG.16197.14,ENST00000599345	MSTRG.16197.5	12	-	19	4123783	4117630	4117629	4117419	4117629	4117419	4117418	4114121	2	2	1	6153	210	210	3297	210	4725	211	1
ENSG00000113163:-:5:75411010:75426478	ENSG00000113163	ENST00000644072,ENST00000647512,ENST00000644516,ENST00000646713,ENST00000642556,ENST00000643780,MSTRG.26421.15,ENST00000645483,ENST00000646511,ENST00000642225,ENST00000643158,ENST00000405807,ENST00000644912,ENST00000261415,ENST00000644445,ENST00000642809,MSTRG.26421.20,ENST00000644377	ENST00000644072	18	-	5	75459064	75426479	75426478	75426371	75411103	75411011	75411010	75403059	4	8	5	32585	107	92	7951	99.5	20268	582	1
ENSG00000147050:+:X:44961283:44974715	ENSG00000147050	ENST00000674739,ENST00000675816,ENST00000676062,ENST00000611820,ENST00000676389,ENST00000674586,ENST00000382899,ENST00000377967,ENST00000675514,ENST00000675577,ENST00000674564,ENST00000676343,ENST00000536777,MSTRG.34148.18,ENST00000543216,ENST00000674541,ENST00000674659,MSTRG.34148.21,ENST00000675182,ENST00000676085,ENST00000684352,MSTRG.34148.23,ENST00000683021,ENST00000682908,MSTRG.34148.24,ENST00000675157,ENST00000675440,ENST00000621147,ENST00000475233	ENST00000674739	28	+	X	44873988	44961283	44961284	44961392	44974666	44974715	44974716	45010960	3	4	2	87295	108	49	36244	78.5	61769.5	159	1
ENSG00000139734:-:13:59774188:59924870	ENSG00000139734	ENST00000400324,ENST00000377908,ENST00000400320,ENST00000400319	ENST00000400324	28	-	13	59969943	59924871	59924870	59924775	59774248	59774189	59774188	59666847	18	27	10	45072	95	59	107341	77	76206.5	1245	1
ENSG00000259024:-:17:15503097:15503232	ENSG00000259024	MSTRG.13711.6,ENST00000557349	MSTRG.13711.6	9	-	17	15540432	15503233	15503232	15503098	15503232	15503098	15503097	15440286	7	7	1	37199	134	134	62811	134	50005	135	1
ENSG00000151466:-:4:128936654:128943188	ENSG00000151466	ENST00000506233,ENST00000281142,ENST00000502495	ENST00000506233	11	-	4	128946006	128943189	128943188	128942996	128936851	128936655	128936654	128891138	7	8	2	2817	192	196	45516	194	24166.5	390	1
ENSG00000168003:+:11:62884456:62884525	ENSG00000168003	ENST00000681563,ENST00000680654,ENST00000680002,MSTRG.5646.3,ENST00000538084,ENST00000681569,MSTRG.5646.7,MSTRG.5646.4,ENST00000680729,ENST00000377890,ENST00000377891,ENST00000681467,ENST00000680725,MSTRG.5646.6,MSTRG.5646.8,ENST00000681215,ENST00000535296,MSTRG.5646.5,ENST00000680297,ENST00000544377,ENST00000541425,ENST00000377889,MSTRG.5646.9,ENST00000680610,ENST00000681107,ENST00000541649,ENST00000681232,ENST00000680134,ENST00000338663,ENST00000680631,MSTRG.5646.17,ENST00000538682,ENST00000535768,ENST00000539891,ENST00000679594,ENST00000536981,ENST00000457660,ENST00000546312,ENST00000537508,ENST00000546253	ENST00000681563	7	+	11	62883000	62884456	62884457	62884525	62884457	62884525	62884526	62884631	3	3	1	1456	68	68	105	68	780.5	69	1
ENSG00000125826:+:20:417525:429094	ENSG00000125826	ENST00000382214,ENST00000415942,ENST00000640614,ENST00000356286,ENST00000353660,MSTRG.20225.1,ENST00000382181	ENST00000382214	12	+	20	410026	417525	417526	417619	428951	429094	429095	430349	3	11	9	7499	93	143	1254	118	4376.5	1260	1
ENSG00000125351:-:X:119837756:119838527	ENSG00000125351	ENST00000276201,ENST00000345865,MSTRG.34799.2,MSTRG.34799.4,MSTRG.34799.3	ENST00000276201	11	-	X	119840645	119838528	119838527	119838367	119838051	119837757	119837756	119835028	9	10	2	2117	160	294	2728	227	2422.5	456	1
ENSG00000099974:+:22:23971285:23971356	ENSG00000099974	MSTRG.21806.2,MSTRG.21806.3	MSTRG.21806.2	4	+	22	23967562	23971285	23971286	23971356	23971286	23971356	23971357	23971435	3	3	1	3723	70	70	78	70	1900.5	71	1
ENSG00000033170:+:14:65455620:65629606	ENSG00000033170	ENST00000360689,ENST00000673929,ENST00000674118,ENST00000358307,ENST00000342677,ENST00000557164	ENST00000360689	11	+	14	65411994	65455620	65455621	65455718	65629492	65629606	65629607	65669242	2	6	5	43626	97	114	39635	105.5	41630.5	922	1
ENSG00000113643:+:5:168488601:168492847	ENSG00000113643	MSTRG.27275.1,MSTRG.27275.3,ENST00000231572,ENST00000520013,MSTRG.27275.5,MSTRG.27275.9,MSTRG.27275.2,ENST00000521939,ENST00000521329	MSTRG.27275.1	16	+	5	168486544	168488601	168488602	168488736	168492659	168492847	168492848	168493893	3	4	2	2057	134	188	1045	161	1551	324	1
ENSG00000180198_ENSG00000180098:+:1:28529857:28553737	ENSG00000180198_ENSG00000180098	MSTRG.692.8	MSTRG.692.8	22	+	1	28518309	28529857	28529858	28529939	28553640	28553737	28553738	28560632	6	15	10	11548	81	97	6894	89	9221	2110	1
ENSG00000127663:+:19:5047475:5082504	ENSG00000127663	ENST00000611640,ENST00000159111,MSTRG.16238.4,ENST00000536461,ENST00000381759	ENST00000611640	24	+	19	5041252	5047475	5047476	5047669	5082367	5082504	5082505	5110621	6	9	4	6223	193	137	28116	165	17169.5	486	1
ENSG00000104375:-:8:98706466:98707311	ENSG00000104375	ENST00000523601,ENST00000419617,ENST00000424861	ENST00000523601	13	-	8	98749275	98707312	98707311	98707147	98706634	98706467	98706466	98596170	7	8	2	41963	164	167	110296	165.5	76129.5	333	1
ENSG00000102181:-:X:150795205:150795486	ENSG00000102181	ENST00000346693,ENST00000613030,ENST00000370377,MSTRG.35038.5,ENST00000466436,ENST00000418547,ENST00000634795	ENST00000346693	12	-	X	150814861	150795487	150795486	150795418	150795289	150795206	150795205	150793757	6	7	2	19374	68	83	1448	75.5	10411	153	1
ENSG00000144224:+:2:135748266:135748369	ENSG00000144224	MSTRG.19269.2,ENST00000272638,MSTRG.19269.1,ENST00000416538,ENST00000415164,ENST00000426921,ENST00000467065	MSTRG.19269.2	14	+	2	135742012	135748266	135748267	135748369	135748267	135748369	135748370	135753538	2	2	1	6254	102	102	5168	102	5711	103	1
ENSG00000259112:-:11:78072997:78073141	ENSG00000259112	ENST00000530054,ENST00000612612	ENST00000530054	3	-	11	78079578	78073142	78073141	78072998	78073141	78072998	78072997	78017271	2	2	1	6436	143	143	55726	143	31081	144	1
ENSG00000106066:-:7:29030576:29096217	ENSG00000106066	ENST00000409850,ENST00000265394,ENST00000396276,MSTRG.29645.2	ENST00000409850	17	-	7	29112703	29096218	29096217	29096103	29030759	29030577	29030576	28995883	8	16	9	16485	114	182	34693	148	25589	1032	1
ENSG00000136021:+:12:100312431:100317925	ENSG00000136021	ENST00000360820,MSTRG.7866.1,ENST00000635101,MSTRG.7866.3,ENST00000549687	ENST00000360820	18	+	12	100311194	100312431	100312432	100312653	100317803	100317925	100317926	100323524	6	10	5	1237	221	122	5598	171.5	3417.5	765	1
ENSG00000014824:+:4:42001615:42001780	ENSG00000014824	ENST00000264451,MSTRG.24796.2,MSTRG.24796.1,MSTRG.24796.6,MSTRG.24796.3,ENST00000513699,MSTRG.24796.5,ENST00000510460	ENST00000264451	18	+	4	41990761	42001615	42001616	42001780	42001616	42001780	42001781	42018110	2	2	1	10854	164	164	16329	164	13591.5	165	1
ENSG00000065243:+:1:88740987:88786213	ENSG00000065243	MSTRG.1553.1,ENST00000370521,MSTRG.1553.2,MSTRG.1553.7,ENST00000370513,MSTRG.1553.8,ENST00000316005	MSTRG.1553.1	22	+	1	88684629	88740987	88740988	88741288	88786104	88786213	88786214	88804390	2	8	7	56358	300	109	18176	204.5	37267	1185	1
ENSG00000106723:+:9:88415606:88426591	ENSG00000106723	ENST00000469017,MSTRG.32951.5,MSTRG.32951.7	ENST00000469017	9	+	9	88388539	88415606	88415607	88415699	88426382	88426591	88426592	88448940	2	5	4	27067	92	209	22348	150.5	24707.5	600	1
ENSG00000204469:+:6:31623731:31628239	ENSG00000204469	MSTRG.28032.2,MSTRG.28032.4,ENST00000376033,ENST00000376007,MSTRG.28032.1,MSTRG.28032.3	MSTRG.28032.2	31	+	6	31622902	31623731	31623732	31623909	31627765	31628239	31628240	31629143	3	12	10	829	177	474	903	325.5	866	1650	1
ENSG00000140391:-:15:77052384:77056255	ENSG00000140391	ENST00000267970,ENST00000346495,ENST00000561277,ENST00000558745	ENST00000267970	7	-	15	77070891	77056256	77056255	77056064	77052468	77052385	77052384	77046928	2	6	5	14635	191	83	5456	137	10045.5	606	1
ENSG00000140374:-:15:76274411:76292700	ENSG00000140374	ENST00000688154,ENST00000690610,ENST00000693064,ENST00000692691,ENST00000687975,ENST00000691021,ENST00000687293,ENST00000557943,ENST00000689730,ENST00000691695,ENST00000688389,ENST00000688908,ENST00000685863,ENST00000559075,ENST00000560595,ENST00000565910,ENST00000689739,ENST00000685548,ENST00000433983,ENST00000560044,ENST00000685118,ENST00000559386,ENST00000688637,ENST00000560345	ENST00000688154	12	-	15	76295590	76292701	76292700	76292619	76274494	76274412	76274411	76231399	3	9	7	2889	81	82	43012	81.5	22950.5	630	1
ENSG00000180376:+:3:56566593:56567053	ENSG00000180376	MSTRG.23130.17,MSTRG.23130.8,MSTRG.23130.7,MSTRG.23130.18,MSTRG.23130.10,MSTRG.23130.23,MSTRG.23130.11,ENST00000471681,ENST00000394672,MSTRG.23130.15,ENST00000326595,ENST00000341455,MSTRG.23130.9,MSTRG.23130.2,ENST00000434467,MSTRG.23130.6,ENST00000442522,ENST00000422222,MSTRG.23130.16	MSTRG.23130.17	17	+	3	56564126	56566593	56566594	56566759	56566950	56567053	56567054	56571180	6	7	2	2467	165	103	4126	134	3296.5	270	1
ENSG00000130695:+:1:26257596:26259802	ENSG00000130695	MSTRG.607.1,ENST00000252992,ENST00000451429,ENST00000640292	MSTRG.607.1	15	+	1	26255866	26257596	26257597	26257730	26259617	26259802	26259803	26268482	6	8	3	1730	133	185	8679	159	5204.5	438	1
ENSG00000121774:+:1:32030297:32033334	ENSG00000121774	ENST00000307714,ENST00000327300,MSTRG.757.4,MSTRG.757.1,ENST00000484270,ENST00000492989	ENST00000307714	9	+	1	32014378	32030297	32030298	32030422	32033188	32033334	32033335	32036909	2	4	3	15919	124	146	3574	135	9746.5	389	1
ENSG00000116221:+:1:54205017:54205410	ENSG00000116221	MSTRG.1246.2,MSTRG.1246.5,MSTRG.1246.10,ENST00000605337,MSTRG.1246.11,MSTRG.1246.3,ENST00000360840,MSTRG.1246.14,MSTRG.1246.4,MSTRG.1246.6,MSTRG.1246.9,ENST00000487096,ENST00000490670	MSTRG.1246.2	8	+	1	54200590	54205017	54205018	54205201	54205295	54205410	54205411	54207359	2	3	2	4427	183	115	1948	149	3187.5	300	1
ENSG00000198160:+:1:66958058:66959743	ENSG00000198160	ENST00000357692,ENST00000401041,ENST00000355356,MSTRG.1372.4,MSTRG.1372.13,MSTRG.1372.12,ENST00000371018,MSTRG.1372.24,MSTRG.1372.5,MSTRG.1372.3,ENST00000371016,MSTRG.1372.14,ENST00000371014,ENST00000401042,ENST00000355977,MSTRG.1372.9,MSTRG.1372.11	ENST00000357692	15	+	1	66946296	66958058	66958059	66958220	66959679	66959743	66959744	66963087	6	8	3	11762	161	64	3343	112.5	7552.5	360	1
ENSG00000186522:-:2:109564365:109585838	ENSG00000186522	ENST00000397712,ENST00000356688,ENST00000397714,ENST00000437928,MSTRG.19014.4,ENST00000415095	ENST00000397712	11	-	2	109593050	109585839	109585838	109585721	109564534	109564366	109564365	109553220	3	8	6	7211	117	168	11145	142.5	9178	929	1
ENSG00000152127:+:2:134253094:134254644	ENSG00000152127	MSTRG.19256.9,MSTRG.19256.1,MSTRG.19256.2,MSTRG.19256.7,ENST00000468758	MSTRG.19256.9	18	+	2	134250146	134253094	134253095	134253218	134254265	134254644	134254645	134270385	2	3	2	2948	123	379	15740	251	9344	504	1
ENSG00000145982:+:6:5404541:5431172	ENSG00000145982	ENST00000648580,ENST00000324331,MSTRG.27588.2,ENST00000274680,MSTRG.27588.5,ENST00000445533	ENST00000648580	9	+	6	5369183	5404541	5404542	5404701	5431041	5431172	5431173	5545179	3	4	2	35358	159	131	114006	145	74682	292	1
ENSG00000132153:+:3:47805325:47843255	ENSG00000132153	MSTRG.22894.7,MSTRG.22894.1,ENST00000348968,ENST00000395745,ENST00000445061,MSTRG.22894.15,MSTRG.22894.5,MSTRG.22894.13,MSTRG.22894.2,MSTRG.22894.4	MSTRG.22894.7	24	+	3	47803213	47805325	47805326	47805420	47843106	47843255	47843256	47845699	2	11	10	2112	94	149	2443	121.5	2277.5	1571	1
ENSG00000177479:+:3:48922747:48927813	ENSG00000177479	MSTRG.22928.3,MSTRG.22928.5,MSTRG.22928.6,MSTRG.22928.2,MSTRG.22928.19,MSTRG.22928.28,MSTRG.22928.7,ENST00000449376,MSTRG.22928.30,ENST00000356401,ENST00000420814,MSTRG.22928.27,ENST00000430423,ENST00000449729	MSTRG.22928.3	17	+	3	48918999	48922747	48922748	48922811	48927462	48927813	48927814	48961611	2	4	3	3748	63	351	33797	207	18772.5	670	1
ENSG00000126016:-:X:112804946:112832370	ENSG00000126016	ENST00000371959,MSTRG.34738.5,MSTRG.34738.3,ENST00000304758,MSTRG.34738.4,MSTRG.34738.9	ENST00000371959	14	-	X	112840451	112832371	112832370	112832294	112805092	112804947	112804946	112791982	2	8	7	8080	76	145	12964	110.5	10522	2064	1
ENSG00000198887:+:9:70297965:70298221	ENSG00000198887	ENST00000361138,MSTRG.32851.1,MSTRG.32851.3,MSTRG.32851.5	ENST00000361138	25	+	9	70286272	70297965	70297966	70298221	70297966	70298221	70298222	70300045	9	9	1	11693	255	255	1823	255	6758	256	1
ENSG00000005810:-:13:77055557:77062697	ENSG00000005810	ENST00000682321,ENST00000683823,ENST00000695079,ENST00000684354,ENST00000695078,ENST00000683697,ENST00000357337,ENST00000544440,MSTRG.8919.6,MSTRG.8919.14,ENST00000695080,MSTRG.8919.17,MSTRG.8919.15,ENST00000695081,MSTRG.8919.19,MSTRG.8919.18,MSTRG.8919.20,ENST00000695083,ENST00000429715	ENST00000682321	85	-	13	77064614	77062698	77062697	77062596	77055767	77055558	77055557	77051919	76	82	7	1916	101	209	3638	155	2777	975	1
ENSG00000055917:-:2:20318536:20327378	ENSG00000055917	MSTRG.18016.1,ENST00000361078,MSTRG.18016.4,MSTRG.18016.5,MSTRG.18016.7,MSTRG.18016.9,MSTRG.18016.8,ENST00000442400,ENST00000432105,ENST00000420234,ENST00000446940	MSTRG.18016.1	24	-	2	20331716	20327379	20327378	20327310	20318645	20318537	20318536	20312424	5	6	2	4337	68	108	6112	88	5224.5	178	1
ENSG00000082898:-:2:61522610:61533903	ENSG00000082898	ENST00000428210,ENST00000676667,ENST00000677290,MSTRG.18496.4,ENST00000679035,ENST00000678360,ENST00000401558,ENST00000676783,ENST00000678182,ENST00000677150,ENST00000678172,ENST00000677239,ENST00000678081,ENST00000677928,ENST00000677814,ENST00000678790,ENST00000676771,MSTRG.18496.38,MSTRG.18496.3,ENST00000676553,ENST00000677813,MSTRG.18496.42,ENST00000676889,ENST00000676789,MSTRG.18496.35,ENST00000677417,ENST00000678741,ENST00000680228,ENST00000676999,ENST00000678263,ENST00000677422,ENST00000678877,ENST00000404992,ENST00000406957,ENST00000677850,ENST00000451765,ENST00000443240	ENST00000428210	24	-	2	61537561	61533904	61533903	61533772	61522683	61522611	61522610	61502311	2	5	4	3657	131	72	20299	101.5	11978	1299	1
ENSG00000064393:-:7:139715931:139717015	ENSG00000064393	ENST00000406875,MSTRG.30809.2,ENST00000428878,MSTRG.30809.4,ENST00000342645	ENST00000406875	15	-	7	139777604	139717016	139717015	139715932	139717015	139715932	139715931	139631726	2	2	1	60588	1083	1083	84205	1083	72396.5	1084	1
ENSG00000184863:+:7:155672866:155680908	ENSG00000184863	MSTRG.30985.1,MSTRG.30985.7,MSTRG.30985.5,ENST00000401878,MSTRG.30985.6,MSTRG.30985.3,ENST00000287912,ENST00000392759	MSTRG.30985.1	18	+	7	155665254	155672866	155672867	155672915	155680590	155680908	155680909	155700772	3	5	3	7612	48	318	19863	183	13737.5	445	1
ENSG00000169398:-:8:140846598:140890769	ENSG00000169398	ENST00000696786,ENST00000522684,ENST00000517887,ENST00000340930,ENST00000521059,MSTRG.32160.5,ENST00000395218,ENST00000519419,MSTRG.32160.15,ENST00000519993,MSTRG.32160.9,ENST00000519654,MSTRG.32160.18,MSTRG.32160.22,MSTRG.32160.19,MSTRG.32160.24,MSTRG.32160.27,MSTRG.32160.20,MSTRG.32160.23,MSTRG.32160.25,ENST00000521791,ENST00000524357	ENST00000696786	36	-	8	140925660	140890770	140890769	140890543	140846678	140846599	140846598	140846323	3	6	4	34890	226	79	275	152.5	17582.5	562	1
ENSG00000145495:+:5:10415487:10417404	ENSG00000145495	ENST00000274140,ENST00000511802,MSTRG.25940.1,ENST00000449913,ENST00000503788,MSTRG.25940.10,MSTRG.25940.9,ENST00000510792	ENST00000274140	26	+	5	10414503	10415487	10415488	10415669	10417270	10417404	10417405	10423734	21	22	2	984	181	134	6329	157.5	3656.5	317	1
ENSG00000132842:-:5:78089392:78116234	ENSG00000132842	ENST00000519295,ENST00000695505,ENST00000695451,ENST00000695507,ENST00000695455,ENST00000695511,ENST00000695488,ENST00000695454,ENST00000255194,ENST00000695513,ENST00000695453,ENST00000695515,ENST00000695510,ENST00000519888,ENST00000695450	ENST00000519295	27	-	5	78128029	78116235	78116234	78116126	78089499	78089393	78089392	78039275	18	22	5	11794	108	106	50117	107	30955.5	609	1
ENSG00000118420:-:6:82957311:83044659	ENSG00000118420	ENST00000369747,ENST00000237186,MSTRG.28587.4,ENST00000430071,ENST00000509102	ENST00000369747	10	-	6	83054147	83044660	83044659	83044428	82957450	82957312	82957311	82893043	4	9	6	9487	231	138	64268	184.5	36877.5	784	1
ENSG00000229807:-:X:73829067:73831274	ENSG00000229807	ENST00000429829,ENST00000650627,ENST00000648970,ENST00000647913,ENST00000648991,ENST00000647696,MSTRG.34462.23,ENST00000669898,ENST00000648927,ENST00000650548,ENST00000648607,ENST00000421322,ENST00000666309,MSTRG.34462.12,MSTRG.34462.1,MSTRG.34462.5,ENST00000635841,ENST00000434839,MSTRG.34462.33,MSTRG.34462.28,ENST00000602587,MSTRG.34462.30,ENST00000445814	ENST00000429829	6	-	X	73833237	73831275	73831274	73831066	73829231	73829068	73829067	73827985	4	5	2	1962	208	163	1082	185.5	1522	373	1
ENSG00000163738:+:4:74174505:74225394	ENSG00000163738	MSTRG.25035.10,ENST00000359107,ENST00000395759,ENST00000325278,ENST00000429519,ENST00000433372,MSTRG.25035.8,MSTRG.25035.14,MSTRG.25035.9	MSTRG.25035.10	9	+	4	74158282	74174505	74174506	74174690	74225302	74225394	74225395	74281424	2	7	6	16223	184	92	56029	138	36126	762	1
ENSG00000153147:+:4:143543508:143543972	ENSG00000153147	ENST00000283131	ENST00000283131	24	+	4	143540496	143543508	143543509	143543657	143543853	143543972	143543973	143544736	15	16	2	3012	148	119	763	133.5	1887.5	269	1
ENSG00000106100:-:7:30446140:30455311	ENSG00000106100	MSTRG.29665.1,MSTRG.29665.2,MSTRG.29665.4,ENST00000434755,ENST00000222823,MSTRG.29665.3,MSTRG.29665.5,MSTRG.29665.11,MSTRG.29665.10,MSTRG.29665.9	MSTRG.29665.1	16	-	7	30456720	30455312	30455311	30455137	30446224	30446141	30446140	30437657	6	11	6	1408	174	83	8483	128.5	4945.5	2116	1
ENSG00000048405:-:7:127373341:127377329	ENSG00000048405	ENST00000265827,ENST00000393313,MSTRG.30605.1,ENST00000393312,MSTRG.30605.3	ENST00000265827	6	-	7	127386059	127377330	127377329	127377186	127375034	127373342	127373341	127371815	4	5	2	8729	143	1692	1526	917.5	5127.5	1837	1
ENSG00000164944:-:8:94530962:94538346	ENSG00000164944	ENST00000297591,MSTRG.31824.4,MSTRG.31824.9,MSTRG.31824.7,MSTRG.31824.8,MSTRG.31824.6,ENST00000421249,ENST00000519001	ENST00000297591	24	-	8	94543826	94538347	94538346	94538260	94531085	94530963	94530962	94529343	3	6	4	5479	86	122	1619	104	3549	428	1
ENSG00000104375:-:8:98526741:98548161	ENSG00000104375	ENST00000523601,ENST00000419617,ENST00000617590	ENST00000523601	13	-	8	98579663	98548162	98548161	98547969	98526917	98526742	98526741	98456001	11	12	2	31501	192	175	70740	183.5	51120.5	369	1
ENSG00000170310:-:17:9378551:9378653	ENSG00000170310	MSTRG.13639.1,ENST00000306357,ENST00000574431,ENST00000575858,ENST00000575294,ENST00000570583	MSTRG.13639.1	9	-	17	9491828	9378654	9378653	9378552	9378653	9378552	9378551	9250646	7	7	1	113174	101	101	127905	101	120539.5	102	1
ENSG00000145996:+:6:21000226:21108463	ENSG00000145996	ENST00000274695,ENST00000378610	ENST00000274695	16	+	6	20955586	21000226	21000227	21000372	21108401	21108463	21108464	21198020	11	13	3	44640	145	62	89556	103.5	67098	390	1
ENSG00000112701:+:6:75621531:75647801	ENSG00000112701	ENST00000370010,ENST00000447266,MSTRG.28542.4,MSTRG.28542.10,MSTRG.28542.8,MSTRG.28542.6,MSTRG.28542.12,MSTRG.28542.5,MSTRG.28542.11,MSTRG.28542.7,MSTRG.28542.9,ENST00000327284,ENST00000483859	ENST00000370010	23	+	6	75602577	75621531	75621532	75621625	75647731	75647801	75647802	75659261	2	6	5	18954	93	70	11459	81.5	15206.5	477	1
ENSG00000149311:+:11:108315822:108335961	ENSG00000149311	ENST00000683174,MSTRG.6277.10,ENST00000452508,ENST00000675843,ENST00000278616,MSTRG.6277.12,MSTRG.6277.5,ENST00000675595,ENST00000527805,ENST00000684152,ENST00000683524,ENST00000682286,ENST00000682302,ENST00000524792	ENST00000683174	63	+	11	108312499	108315822	108315823	108315911	108335845	108335961	108335962	108343221	41	56	16	3323	88	116	7259	102	5291	2262	1
ENSG00000140374:-:15:76274411:76295737	ENSG00000140374	ENST00000688154,ENST00000690610,ENST00000693064,ENST00000692691,ENST00000687975,ENST00000691021,ENST00000687293,ENST00000557943,ENST00000689730,ENST00000688389,ENST00000559075,ENST00000560595,ENST00000565910,ENST00000689739,ENST00000685548,ENST00000560044,ENST00000685118,ENST00000267950,ENST00000559386,ENST00000688637,ENST00000691071	ENST00000688154	12	-	15	76311349	76295738	76295737	76295591	76274494	76274412	76274411	76231399	2	9	8	15611	146	82	43012	114	29311.5	777	1
ENSG00000139746:-:13:79334343:79337302	ENSG00000139746	MSTRG.8946.3,MSTRG.8946.4,MSTRG.8946.7,MSTRG.8946.6,MSTRG.8946.8,MSTRG.8946.1,MSTRG.8946.11,MSTRG.8946.10,MSTRG.8946.5,MSTRG.8946.12,MSTRG.8946.14,MSTRG.8946.17,ENST00000622611,ENST00000438737,ENST00000438724,MSTRG.8946.19,MSTRG.8946.32,MSTRG.8946.22,MSTRG.8946.9,MSTRG.8946.30,MSTRG.8946.21,MSTRG.8946.27,MSTRG.8946.29,MSTRG.8946.26,MSTRG.8946.31,MSTRG.8946.13,MSTRG.8946.15,MSTRG.8946.16,MSTRG.8946.18,ENST00000267229,MSTRG.8946.37,MSTRG.8946.23,ENST00000449987,MSTRG.8946.25,MSTRG.8946.28,MSTRG.8946.20,MSTRG.8946.24	MSTRG.8946.3	23	-	13	79341122	79337303	79337302	79337102	79334430	79334344	79334343	79322463	18	19	2	3819	200	86	11880	143	7849.5	288	1
ENSG00000156232:+:15:82813102:82826846	ENSG00000156232	MSTRG.11397.3,ENST00000286760,MSTRG.11397.1,MSTRG.11397.2	MSTRG.11397.3	11	+	15	82810336	82813102	82813103	82813276	82826751	82826846	82826847	82830598	2	9	8	2766	173	95	3751	134	3258.5	1161	1
ENSG00000170681_ENSG00000251349:+:9:100498764:100516771	ENSG00000170681_ENSG00000251349	MSTRG.33150.11,MSTRG.33150.10	MSTRG.33150.11	12	+	9	100442357	100498764	100498765	100498874	100516675	100516771	100516772	100547743	2	5	4	56407	109	96	30971	102.5	43689	364	1
ENSG00000106771:-:9:109064476:109080943	ENSG00000106771	ENST00000374586,MSTRG.33202.2,MSTRG.33202.3,MSTRG.33202.4,ENST00000491854,ENST00000413712	ENST00000374586	18	-	9	109085996	109080944	109080943	109080839	109064567	109064477	109064476	109060453	8	10	3	5052	104	90	4023	97	4537.5	279	1
ENSG00000196814:+:9:126340507:126386658	ENSG00000196814	ENST00000361171,MSTRG.33395.3,ENST00000489637,ENST00000402437	ENST00000361171	10	+	9	126327011	126340507	126340508	126340630	126386562	126386658	126386659	126392065	2	4	3	13496	122	96	5406	109	9451	328	1
ENSG00000111445:+:12:118019071:118025828	ENSG00000111445	MSTRG.8107.4,ENST00000392542,MSTRG.8107.2,ENST00000454402	MSTRG.8107.4	12	+	12	118016893	118019071	118019072	118019136	118025747	118025828	118025829	118026888	2	7	6	2178	64	81	1059	72.5	1618.5	598	1
ENSG00000090565:+:16:482524:482736	ENSG00000090565	ENST00000262305,MSTRG.11712.4,MSTRG.11712.3,MSTRG.11712.5,ENST00000434585,ENST00000450428,MSTRG.11712.2,MSTRG.11712.6,ENST00000452814,ENST00000449879,ENST00000483002	ENST00000262305	14	+	16	471390	482524	482525	482736	482525	482736	482737	488850	4	4	1	11134	211	211	6113	211	8623.5	212	1
ENSG00000141127:+:17:18865468:18911251	ENSG00000141127	MSTRG.13816.21,MSTRG.13816.23,MSTRG.13816.10,ENST00000268835,MSTRG.13816.36,MSTRG.13816.30,MSTRG.13816.17,ENST00000610773,MSTRG.13816.15,ENST00000492129,ENST00000419071,ENST00000422237,ENST00000487101,ENST00000455992	MSTRG.13816.21	13	+	17	18859476	18865468	18865469	18865564	18911103	18911251	18911252	18923913	3	10	8	5992	95	148	12661	121.5	9326.5	861	1
ENSG00000198879:-:10:7367648:7381949	ENSG00000198879	ENST00000397167,ENST00000361972,ENST00000684547,ENST00000673876,ENST00000683762,MSTRG.3508.6,ENST00000682896,ENST00000379713,ENST00000682101,ENST00000379711	ENST00000397167	21	-	10	7410860	7381950	7381949	7381799	7367889	7367649	7367648	7285955	2	4	3	28910	150	240	81693	195	55301.5	487	1
ENSG00000092020:-:14:35107303:35110629	ENSG00000092020	MSTRG.9376.1,ENST00000557217,ENST00000554222,ENST00000553273,ENST00000261475,ENST00000554361,ENST00000555630,ENST00000554488,ENST00000555726,ENST00000554563	MSTRG.9376.1	14	-	14	35116609	35110630	35110629	35110525	35107374	35107304	35107303	35099385	4	7	4	5979	104	70	7918	87	6948.5	387	1
ENSG00000085415:+:18:12951854:12978892	ENSG00000085415	MSTRG.15529.1,ENST00000262124,MSTRG.15529.12,MSTRG.15529.7,MSTRG.15529.5,ENST00000399892,ENST00000587761	MSTRG.15529.1	10	+	18	12948233	12951854	12951855	12951905	12978752	12978892	12978893	12982517	3	7	5	3621	50	140	3624	95	3622.5	650	1
ENSG00000141556:+:17:82756164:82800996	ENSG00000141556	ENST00000681983,ENST00000684361,ENST00000684760,ENST00000684349,ENST00000683444,ENST00000682479,ENST00000684464,ENST00000684429,ENST00000539345,ENST00000355528,ENST00000684000,ENST00000683041,ENST00000684544,ENST00000683282,ENST00000683184,ENST00000684188,ENST00000684776,ENST00000682213,ENST00000684408,ENST00000683009,ENST00000682921,ENST00000682107	ENST00000681983	38	+	17	82752378	82756164	82756165	82756215	82800864	82800996	82800997	82805874	2	9	8	3786	50	132	4877	91	4331.5	766	1
ENSG00000102606:+:13:111217678:111244294	ENSG00000102606	ENST00000317133,ENST00000375741,ENST00000646102,ENST00000375739,ENST00000218789,MSTRG.9077.2,ENST00000375736,ENST00000375723,ENST00000426073,MSTRG.9077.14,MSTRG.9077.13,MSTRG.9077.12,MSTRG.9077.9,ENST00000466143	ENST00000317133	19	+	13	111210003	111217678	111217679	111217880	111244199	111244294	111244295	111267547	5	8	4	7675	201	95	23252	148	15463.5	482	1
ENSG00000137815:+:15:41464770:41466252	ENSG00000137815	ENST00000389629,MSTRG.10601.4	ENST00000389629	18	+	15	41457877	41464770	41464771	41464885	41466141	41466252	41466253	41470256	5	6	2	6893	114	111	4003	112.5	5448	227	1
ENSG00000136492:-:17:61847100:61857231	ENSG00000136492	ENST00000682589,ENST00000259008,ENST00000682755,ENST00000682477,ENST00000683039,ENST00000683235,ENST00000682453,MSTRG.14846.10,MSTRG.14846.11,ENST00000682989,MSTRG.14846.12,ENST00000577598,MSTRG.14846.19,ENST00000584322,ENST00000683381,ENST00000577913,ENST00000682369	ENST00000682589	17	-	17	61859795	61857232	61857231	61857058	61847220	61847101	61847100	61808758	3	5	3	2563	173	119	38342	146	20452.5	422	1
ENSG00000239900:+:22:40353072:40363071	ENSG00000239900	ENST00000623063,ENST00000681159,ENST00000680978,ENST00000637666,ENST00000480775,ENST00000680444,ENST00000625194,ENST00000680378,ENST00000342312,ENST00000637669,ENST00000636714	ENST00000623063	13	+	22	40350036	40353072	40353073	40353117	40362981	40363071	40363072	40364275	3	10	8	3036	44	90	1203	67	2119.5	744	1
ENSG00000116711:+:1:186977592:186979472	ENSG00000116711	ENST00000367466	ENST00000367466	18	+	1	186965594	186977592	186977593	186977788	186979315	186979472	186979473	186988376	16	17	2	11998	195	157	8903	176	10450.5	354	1
ENSG00000106443:+:7:10990702:11022979	ENSG00000106443	ENST00000642461,ENST00000403050,ENST00000490957,ENST00000634607,ENST00000521747,MSTRG.29511.9,ENST00000423760,MSTRG.29511.5,ENST00000476009	ENST00000642461	16	+	7	10974946	10990702	10990703	10990847	11022868	11022979	11022980	11028680	3	5	3	15756	144	111	5700	127.5	10728	417	1
ENSG00000164649:-:7:21908420:21911754	ENSG00000164649	ENST00000435031	ENST00000435031	4	-	7	21916753	21911755	21911754	21911617	21908507	21908421			3	4	2	4998	137	86		111.5	4998	225	1
ENSG00000111880:-:6:88769773:88904955	ENSG00000111880	ENST00000369485,ENST00000627296,ENST00000369475,ENST00000538899	ENST00000369485	16	-	6	88906364	88904956	88904955	88904715	88890596	88890495	88890494	88853765	6	8	3	1408	240	101	36729	170.5	19068.5	453	1
ENSG00000114541:-:3:69432633:69432761	ENSG00000114541	ENST00000459638,ENST00000497757,ENST00000497880	ENST00000459638	6	-	3	69542205	69432762	69432761	69432634	69432761	69432634	69432633	69313518	2	2	1	109443	127	127	119115	127	114279	128	1
ENSG00000143653:+:1:246726891:246736055	ENSG00000143653	ENST00000366510,MSTRG.3433.6,MSTRG.3433.1,MSTRG.3433.5,MSTRG.3433.3	ENST00000366510	12	+	1	246724613	246726891	246726892	246727004	246735975	246736055	246736056	246740171	2	3	2	2278	112	80	4115	96	3196.5	194	1
ENSG00000144749:-:3:66405197:66462509	ENSG00000144749	ENST00000273261,ENST00000383703,MSTRG.23216.5,MSTRG.23216.4	ENST00000273261	19	-	3	66500189	66462510	66462509	66462438	66405278	66405198	66405197	66399042	2	9	8	37679	71	80	6155	75.5	21917	942	1
ENSG00000135387:+:11:34071725:34076642	ENSG00000135387	MSTRG.5367.1,ENST00000532820,ENST00000341394,MSTRG.5367.7,ENST00000529307,MSTRG.5367.8,ENST00000389645,ENST00000530820,ENST00000530008,ENST00000534042	MSTRG.5367.1	17	+	11	34052637	34071725	34071726	34071788	34076560	34076642	34076643	34079627	3	6	4	19088	62	82	2984	72	11036	472	1
ENSG00000156471:+:8:96295097:96295256	ENSG00000156471	ENST00000517309,ENST00000337004,MSTRG.31858.3,ENST00000518776	ENST00000517309	13	+	8	96287147	96295097	96295098	96295256	96295098	96295256	96295257	96299693	5	5	1	7950	158	158	4436	158	6193	159	1
ENSG00000145147:+:4:20256671:20268881	ENSG00000145147	ENST00000504154,ENST00000503823,ENST00000622093,ENST00000273739,MSTRG.24678.9,ENST00000503837,MSTRG.24678.11,MSTRG.24678.2,MSTRG.24678.7,MSTRG.24678.8,MSTRG.24678.10,MSTRG.24678.3,ENST00000508824	ENST00000504154	37	+	4	20253995	20256671	20256672	20256743	20268810	20268881	20268882	20467751	2	4	3	2676	71	71	198869	71	100772.5	216	1
ENSG00000198589:-:4:150302624:150590859	ENSG00000198589	ENST00000357115,ENST00000651943,MSTRG.25594.4,ENST00000510413,MSTRG.25594.7,MSTRG.25594.11,ENST00000651035,MSTRG.25594.15,ENST00000651695,ENST00000509835	ENST00000357115	58	-	4	150597082	150590860	150590859	150590713	150302792	150302625	150302624	150286035	40	54	15	6222	146	167	16589	156.5	11405.5	1971	1
ENSG00000139163:+:12:22643762:22673660	ENSG00000139163	ENST00000671733,ENST00000538218,ENST00000266517,ENST00000673406	ENST00000671733	8	+	12	22625587	22643762	22643763	22644022	22673500	22673660	22673661	22684482	2	6	5	18175	259	160	10821	209.5	14498	789	1
ENSG00000135049:-:9:85692688:85692813	ENSG00000135049	ENST00000357081,ENST00000337006,ENST00000376083,ENST00000628899,MSTRG.32921.6,MSTRG.32921.5,MSTRG.32921.7,MSTRG.32921.8,ENST00000376081,ENST00000376080,ENST00000491784	ENST00000357081	26	-	9	85712501	85692814	85692813	85692689	85692813	85692689	85692688	85681336	3	3	1	19687	124	124	11352	124	15519.5	125	1
ENSG00000083642:+:13:32648753:32652007	ENSG00000083642	ENST00000315596,MSTRG.8571.2,MSTRG.8571.5,MSTRG.8571.4,ENST00000450460,MSTRG.8571.6,ENST00000498550,ENST00000482955	ENST00000315596	35	+	13	32586594	32648753	32648754	32648880	32651804	32652007	32652008	32658238	2	3	2	62159	126	203	6230	164.5	34194.5	331	1
ENSG00000119487:-:9:125669817:125669907	ENSG00000119487	ENST00000265960,ENST00000350766,ENST00000373511,ENST00000373498,ENST00000373503,MSTRG.33390.12,MSTRG.33390.13,ENST00000394060,MSTRG.33390.10,MSTRG.33390.19,ENST00000373496	ENST00000265960	12	-	9	125672315	125669908	125669907	125669818	125669907	125669818	125669817	125657800	3	3	1	2407	89	89	12017	89	7212	90	1
ENSG00000119487:-:9:125669817:125672643	ENSG00000119487	ENST00000265960,ENST00000350766,ENST00000373511,ENST00000373498,MSTRG.33390.12,MSTRG.33390.13,ENST00000394060,ENST00000373496	ENST00000265960	12	-	9	125706970	125672644	125672643	125672316	125669907	125669818	125669817	125657800	2	3	2	34326	327	89	12017	208	23171.5	418	1
ENSG00000105221:-:19:40237968:40242069	ENSG00000105221	ENST00000392038,ENST00000424901,ENST00000476266,MSTRG.17169.4,MSTRG.17169.3,MSTRG.17169.10,MSTRG.17169.13,MSTRG.17169.12,ENST00000579047,ENST00000391844,ENST00000584288,ENST00000311278,ENST00000578615	ENST00000392038	14	-	19	40242533	40242070	40242069	40241938	40238091	40237969	40237968	40236386	6	9	4	463	131	122	1582	126.5	1022.5	390	1
ENSG00000119487:-:9:125657650:125672643	ENSG00000119487	ENST00000265960,ENST00000350766,ENST00000373511,ENST00000373498,MSTRG.33390.12,MSTRG.33390.13,ENST00000394060	ENST00000265960	12	-	9	125706970	125672644	125672643	125672316	125657799	125657651	125657650	125585728	2	4	3	34326	327	148	71922	237.5	53124	567	1
ENSG00000172172:-:8:120413990:120425366	ENSG00000172172	ENST00000306185,ENST00000518696,MSTRG.32022.4,ENST00000518918,MSTRG.32022.5	ENST00000306185	7	-	8	120432029	120425367	120425366	120425306	120414112	120413991	120413990	120396126	4	6	3	6662	60	121	17864	90.5	12263	270	1
ENSG00000083845:+:19:58392975:58393487	ENSG00000083845	MSTRG.17847.3,MSTRG.17847.4,ENST00000596046,MSTRG.17847.1,MSTRG.17847.6,MSTRG.17847.7,MSTRG.17847.5,ENST00000601521,MSTRG.17847.2,MSTRG.17847.11,MSTRG.17847.8,MSTRG.17847.12,MSTRG.17847.13,ENST00000598495,ENST00000596314,ENST00000196551	MSTRG.17847.3	9	+	19	58388246	58392975	58392976	58393185	58393359	58393487	58393488	58394496	6	7	2	4729	209	128	1008	168.5	2868.5	339	1
ENSG00000129515:-:14:34581560:34609742	ENSG00000129515	ENST00000396526,ENST00000362031,ENST00000652385,MSTRG.9362.4,ENST00000556162,MSTRG.9362.5,ENST00000555416	ENST00000396526	13	-	14	34629906	34609743	34609742	34609638	34581600	34581561	34581560	34575843	2	9	8	20163	104	39	5717	71.5	12940	780	1
ENSG00000124422:-:17:21006895:21007996	ENSG00000124422	ENST00000261497,ENST00000537526,MSTRG.13900.5,MSTRG.13900.4,ENST00000579645,MSTRG.13900.1	ENST00000261497	13	-	17	21011150	21007997	21007996	21007870	21006987	21006896	21006895	21004991	9	10	2	3153	126	91	1904	108.5	2528.5	219	1
ENSG00000005175:-:12:47679492:47697719	ENSG00000005175	ENST00000005386,MSTRG.7172.5,ENST00000432584	ENST00000005386	17	-	12	47701463	47697720	47697719	47697597	47679594	47679493	47679492	47670346	4	12	9	3743	122	101	9146	111.5	6444.5	993	1
ENSG00000141627:-:18:49331863:49379758	ENSG00000141627	ENST00000675505,ENST00000269445,MSTRG.15787.5,MSTRG.15787.7,MSTRG.15787.8,MSTRG.15787.6,ENST00000418472	ENST00000675505	18	-	18	49391592	49379759	49379758	49379665	49332006	49331864	49331863	49286617	4	8	5	11833	93	142	45246	117.5	28539.5	570	1

Supplementary

S1. Source Code

Please check my personal GitHub to find the source code.

S2. Running log

Welcome to Ubuntu 20.04.4 LTS (GNU/Linux 5.13.0-41-generic x86_64)

Documentation: https://help.ubuntu.com
Management: https://landscape.canonical.com
Support: https://ubuntu.com/advantage

50 updates can be applied immediately. To see these additional updates run: apt list –upgradable

20 updates could not be installed automatically. For more details, see /var/log/unattended-upgrades/unattended-upgrades.log Your Hardware Enablement Stack (HWE) is supported until 4月 2025.

[2022年 5月 15日日曜日 14:40:13 JST] Create folder for FastQC of origin and filterd fastq files

[2022年 5月 15日日曜日 14:40:13 JST] Create folder for FastQC of bam files from STAR

[2022年 5月 15日日曜日 14:40:13 JST] Download genome reference and unzip it

[2022年 5月 15日日曜日 14:43:09 JST] Download conding DNA reference and unzip it

[2022年 5月 15日日曜日 14:43:46 JST] Download non-coding RNA reference and unzip it

[2022年 5月 15日日曜日 14:43:58 JST] Download genome annotation and unzip it Writing new genes GTF file (may take 10 minutes for a 1GB input GTF file)… …done

Reading in genome_filtered.gtf Processing… Warning: there are empty ‘gene_name’ attributes, using ‘gene_id’ for them Saving exons to genome_filtered_exons.txt

[2022年 5月 15日日曜日 14:47:09 JST] Downloading Pfam-A.hmm Working… done. Pressed and indexed 19632 HMMs (19632 names and 19632 accessions). Models pressed into binary file: ./Pfam_database/Pfam-A.hmm.h3m SSI index for binary model file: ./Pfam_database/Pfam-A.hmm.h3i Profiles (MSV part) pressed into: ./Pfam_database/Pfam-A.hmm.h3f Profiles (remainder) pressed into: ./Pfam_database/Pfam-A.hmm.h3p

[2022年 5月 15日日曜日 14:48:55 JST] Did not find sample list file, now create it and check the extension of FASTQ files. The extension of all FASTQ files in this folder will be changed to .fastq

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hA_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:55 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_2hB_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hA_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_DMSO_6hB_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_2hA_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_OSMI2_6hA_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:56 JST] Extension chekced for ./HEK293_TMG_2hB_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_1.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_2.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L1.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L1.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L2.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L2.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L3.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L3.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L4.R1.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] Extension chekced for ./HEK293_TMG_6hB_3.L4.R2.fastq.gz, process to the next.

[2022年 5月 15日日曜日 14:48:57 JST] FastQC of origin fastq files

Analysis complete for HEK293_DMSO_2hA_2.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_2.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_1.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_3.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_1.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_3.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_2.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L4.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L2.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L1.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_3.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hB_1.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_2hA_1.L1.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L2.R1.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L2.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L4.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L3.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L4.R1.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_2hB_2.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_1.L3.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L2.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L4.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_2.L3.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L3.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L4.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L2.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L4.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_3.L1.R2.fastq.gz Analysis complete for HEK293_DMSO_6hA_3.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L4.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L2.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L3.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L4.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L2.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L1.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L2.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L1.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_1.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L2.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L2.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L3.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L4.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L2.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L1.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L3.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L3.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L2.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L4.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L1.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L3.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L2.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L4.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L1.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L2.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L4.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_2.L1.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L1.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L2.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L4.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L2.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L4.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L2.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L1.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L3.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L2.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_1.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_3.L4.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L2.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L4.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L1.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L3.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L4.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L4.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L4.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L3.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L3.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L2.R1.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L4.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L4.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L4.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L4.R2.fastq.gz Analysis complete for HEK293_TMG_2hB_3.L4.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L2.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L3.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_3.L3.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L3.R1.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L1.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L2.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L2.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L3.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L3.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_2.L1.R2.fastq.gz Analysis complete for HEK293_OSMI2_2hA_2.L1.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L1.R1.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L3.R2.fastq.gz Analysis complete for HEK293_TMG_6hB_1.L1.R2.fastq.gz Analysis complete for HEK293_OSMI2_6hA_2.L1.R1.fastq.gz Analysis complete for HEK293_OSMI2_6hA_2.L1.R2.fastq.gz

[2022年 5月 15日日曜日 15:18:32 JST] Check FastQC results ./HEK293_DMSO_2hA_1.L1.R1.fastq.gz [2022年 5月 15日日曜日 15:18:32 JST] READ_LENGTH: 75 [2022年 5月 15日日曜日 15:18:32 JST] ADAPTER_QC: pass [2022年 5月 15日日曜日 15:18:32 JST] READ_QC: pass

[2022年 5月 15日日曜日 15:18:32 JST] DO_QC: 1

[2022年 5月 15日日曜日 15:18:32 JST] Some of your fastq files have adapter contamination or reads with too low quality. [2022年 5月 15日日曜日 15:18:32 JST] Create folder for FastQC of qulity trimmed fastq files

[2022年 5月 15日日曜日 15:18:32 JST] Did not find STAR index, now building STAR index: STAR –runThreadN 128 –runMode genomeGenerate –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74/ –genomeFastaFiles genome.fa –sjdbGTFfile genome_filtered.gtf –sjdbOverhang 74 –limitGenomeGenerateRAM 211310333132 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 15:18:32 ….. started STAR run May 15 15:18:32 … starting to generate Genome files May 15 15:19:03 ….. processing annotations GTF May 15 15:19:18 … starting to sort Suffix Array. This may take a long time… May 15 15:19:30 … sorting Suffix Array chunks and saving them to disk… May 15 15:25:23 … loading chunks from disk, packing SA… May 15 15:26:20 … finished generating suffix array May 15 15:26:20 … generating Suffix Array index May 15 15:29:16 … completed Suffix Array index May 15 15:29:17 ….. inserting junctions into the genome indices May 15 15:30:53 … writing Genome to disk … May 15 15:30:55 … writing Suffix Array to disk … May 15 15:31:05 … writing SAindex to disk May 15 15:31:07 ….. finished successfully

[2022年 5月 15日日曜日 15:31:07 JST] Building salmon index **************** *** getDecoy ************* -j = 128 -a = /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genome_filtered.gtf -g = /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genome.fa -t = /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genomeTx.fa -o

= decoys [1/10] Extracting exonic features from the gtf [2/10] Masking the genome fasta [3/10] Aligning transcriptome to genome >>>>>>>>>>>>>>>>>> Reference = [reference.masked.genome.fa] Query = [/mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genomeTx.fa] Kmer size = 16 Window size = 5 Segment length = 500 (read split allowed) Alphabet = DNA Percentage identity threshold = 80% Mapping output file = mashmap.out Filter mode = 1 (1 = map, 2 = one-to-one, 3 = none) Execution threads = 128 >>>>>>>>>>>>>>>>>> INFO, skch::Sketch::build, minimizers picked from reference = 932319207 INFO, skch::Sketch::index, unique minimizers = 284408864 INFO, skch::Sketch::computeFreqHist, Frequency histogram of minimizers = (1, 140256445) … (1047003, 1) INFO, skch::Sketch::computeFreqHist, With threshold 0.001%, ignore minimizers occurring >= 7020 times during lookup. INFO, skch::main, Time spent computing the reference index: 229.305 sec INFO, skch::Map::mapQuery, [count of mapped reads, reads qualified for mapping, total input reads] = [224285, 224654, 267165] INFO, skch::main, Time spent mapping the query : 1288.1 sec INFO, skch::main, mapping results saved in : mashmap.out [4/10] Extracting intervals from mashmap alignments [5/10] Merging the intervals [6/10] Extracting sequences from the genome [7/10] Concatenating to get decoy sequences [8/10] Making gentrome [9/10] Extracting decoy sequence ids [10/10] Removing temporary files Threads = 128 Vertex length = 31 Hash functions = 5 Filter size = 8589934592 Capacity = 2 Files: salmon_index/ref_k31_fixed.fa ——————————————————————————– Round 0, 0:8589934592 Pass Filling Filtering 1 23 18
2 17 1 True junctions count = 2269231 False junctions count = 397935 Hash table size = 2667166 Candidate marks count = 25053468 ——————————————————————————– Reallocating bifurcations time: 1 True marks count: 24590400 Edges construction time: 45 ——————————————————————————– Distinct junctions = 2269231

for info, total work write each : 2.331 total work inram from level 3 : 4.322 total work raw : 25.000 Bitarray 1623037376 bits (100.00 %) (array + ranks ) final hash 6048 bits (0.00 %) (nb in final hash 18)

[2022年 5月 15日日曜日 16:09:05 JST] This folder has 186 fastq files. |—-Now start mapping and counting using STAR and featureCounts [2022年 5月 15日日曜日 16:09:05 JST] Please Check Output Folder: HEK293_DMSO_2hA_1

[2022年 5月 15日日曜日 16:09:05 JST] Please Check Read1 file(s): HEK293_DMSO_2hA_1.L1.R1.fastq.gz,HEK293_DMSO_2hA_1.L2.R1.fastq.gz,HEK293_DMSO_2hA_1.L3.R1.fastq.gz,HEK293_DMSO_2hA_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 16:09:05 JST] Please Check Read2 file(s): HEK293_DMSO_2hA_1.L1.R2.fastq.gz,HEK293_DMSO_2hA_1.L2.R2.fastq.gz,HEK293_DMSO_2hA_1.L3.R2.fastq.gz,HEK293_DMSO_2hA_1.L4.R2.fastq.gz

HEK293_DMSO_2hA_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 16:12:24 JST] Using Salmon to Map sample 1 with 128 CPU cores: HEK293_DMSO_2hA_1.L1.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L2.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L3.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L1.R2_val_2.fq.gz HEK293_DMSO_2hA_1.L2.R2_val_2.fq.gz HEK293_DMSO_2hA_1.L3.R2_val_2.fq.gz HEK293_DMSO_2hA_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 16:13:54 JST] Mapping sample 1 with 128 CPU cores: HEK293_DMSO_2hA_1.L1.R1_val_1.fq.gz,HEK293_DMSO_2hA_1.L2.R1_val_1.fq.gz,HEK293_DMSO_2hA_1.L3.R1_val_1.fq.gz,HEK293_DMSO_2hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L1.R2_val_2.fq.gz,HEK293_DMSO_2hA_1.L2.R2_val_2.fq.gz,HEK293_DMSO_2hA_1.L3.R2_val_2.fq.gz,HEK293_DMSO_2hA_1.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_2hA_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_2hA_1.L1.R1_val_1.fq.gz,HEK293_DMSO_2hA_1.L2.R1_val_1.fq.gz,HEK293_DMSO_2hA_1.L3.R1_val_1.fq.gz,HEK293_DMSO_2hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L1.R2_val_2.fq.gz,HEK293_DMSO_2hA_1.L2.R2_val_2.fq.gz,HEK293_DMSO_2hA_1.L3.R2_val_2.fq.gz,HEK293_DMSO_2hA_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_2hA_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 16:13:54 ….. started STAR run May 15 16:13:54 ….. loading genome May 15 16:14:01 ….. started 1st pass mapping May 15 16:14:44 ….. finished 1st pass mapping May 15 16:14:44 ….. inserting junctions into the genome indices May 15 16:15:39 ….. started mapping May 15 16:16:27 ….. finished mapping May 15 16:16:29 ….. started sorting BAM May 15 16:18:15 ….. finished successfully

[2022年 5月 15日日曜日 16:18:18 JST] Indexes HEK293_DMSO_2hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 16:18:24 JST] Converting HEK293_DMSO_2hA_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 16:19:34 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 16:19:34 JST] Run featureCounts to HEK293_DMSO_2hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 16:19:43 JST] FastQC of trimmed fastq files Analysis complete for HEK293_DMSO_2hA_1.L1.R1_val_1.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L1.R2_val_2.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L2.R1_val_1.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L2.R2_val_2.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L4.R1_val_1.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L3.R1_val_1.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L4.R2_val_2.fq.gz Analysis complete for HEK293_DMSO_2hA_1.L3.R2_val_2.fq.gz

[2022年 5月 15日日曜日 16:20:18 JST] Please Check Output Folder: HEK293_DMSO_2hA_2

[2022年 5月 15日日曜日 16:20:18 JST] Please Check Read1 file(s): HEK293_DMSO_2hA_2.L1.R1.fastq.gz,HEK293_DMSO_2hA_2.L2.R1.fastq.gz,HEK293_DMSO_2hA_2.L3.R1.fastq.gz,HEK293_DMSO_2hA_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 16:20:18 JST] Please Check Read2 file(s): HEK293_DMSO_2hA_2.L1.R2.fastq.gz,HEK293_DMSO_2hA_2.L2.R2.fastq.gz,HEK293_DMSO_2hA_2.L3.R2.fastq.gz,HEK293_DMSO_2hA_2.L4.R2.fastq.gz

HEK293_DMSO_2hA_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 16:22:55 JST] Using Salmon to Map sample 2 with 128 CPU cores: HEK293_DMSO_2hA_2.L1.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L2.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L3.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L1.R2_val_2.fq.gz HEK293_DMSO_2hA_2.L2.R2_val_2.fq.gz HEK293_DMSO_2hA_2.L3.R2_val_2.fq.gz HEK293_DMSO_2hA_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 16:24:17 JST] Mapping sample 2 with 128 CPU cores: HEK293_DMSO_2hA_2.L1.R1_val_1.fq.gz,HEK293_DMSO_2hA_2.L2.R1_val_1.fq.gz,HEK293_DMSO_2hA_2.L3.R1_val_1.fq.gz,HEK293_DMSO_2hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L1.R2_val_2.fq.gz,HEK293_DMSO_2hA_2.L2.R2_val_2.fq.gz,HEK293_DMSO_2hA_2.L3.R2_val_2.fq.gz,HEK293_DMSO_2hA_2.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_2hA_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_2hA_2.L1.R1_val_1.fq.gz,HEK293_DMSO_2hA_2.L2.R1_val_1.fq.gz,HEK293_DMSO_2hA_2.L3.R1_val_1.fq.gz,HEK293_DMSO_2hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L1.R2_val_2.fq.gz,HEK293_DMSO_2hA_2.L2.R2_val_2.fq.gz,HEK293_DMSO_2hA_2.L3.R2_val_2.fq.gz,HEK293_DMSO_2hA_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_2hA_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 16:24:17 ….. started STAR run May 15 16:24:17 ….. loading genome May 15 16:24:24 ….. started 1st pass mapping May 15 16:25:02 ….. finished 1st pass mapping May 15 16:25:03 ….. inserting junctions into the genome indices May 15 16:25:57 ….. started mapping May 15 16:26:38 ….. finished mapping May 15 16:26:39 ….. started sorting BAM May 15 16:28:06 ….. finished successfully

[2022年 5月 15日日曜日 16:28:08 JST] Indexes HEK293_DMSO_2hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 16:28:13 JST] Converting HEK293_DMSO_2hA_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 16:29:12 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 16:29:12 JST] Run featureCounts to HEK293_DMSO_2hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 16:29:21 JST] Please Check Output Folder: HEK293_DMSO_2hA_3

[2022年 5月 15日日曜日 16:29:21 JST] Please Check Read1 file(s): HEK293_DMSO_2hA_3.L1.R1.fastq.gz,HEK293_DMSO_2hA_3.L2.R1.fastq.gz,HEK293_DMSO_2hA_3.L3.R1.fastq.gz,HEK293_DMSO_2hA_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 16:29:21 JST] Please Check Read2 file(s): HEK293_DMSO_2hA_3.L1.R2.fastq.gz,HEK293_DMSO_2hA_3.L2.R2.fastq.gz,HEK293_DMSO_2hA_3.L3.R2.fastq.gz,HEK293_DMSO_2hA_3.L4.R2.fastq.gz

HEK293_DMSO_2hA_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 16:34:09 JST] Using Salmon to Map sample 3 with 128 CPU cores: HEK293_DMSO_2hA_3.L1.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L2.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L3.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L1.R2_val_2.fq.gz HEK293_DMSO_2hA_3.L2.R2_val_2.fq.gz HEK293_DMSO_2hA_3.L3.R2_val_2.fq.gz HEK293_DMSO_2hA_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 16:36:01 JST] Mapping sample 3 with 128 CPU cores: HEK293_DMSO_2hA_3.L1.R1_val_1.fq.gz,HEK293_DMSO_2hA_3.L2.R1_val_1.fq.gz,HEK293_DMSO_2hA_3.L3.R1_val_1.fq.gz,HEK293_DMSO_2hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L1.R2_val_2.fq.gz,HEK293_DMSO_2hA_3.L2.R2_val_2.fq.gz,HEK293_DMSO_2hA_3.L3.R2_val_2.fq.gz,HEK293_DMSO_2hA_3.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_2hA_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_2hA_3.L1.R1_val_1.fq.gz,HEK293_DMSO_2hA_3.L2.R1_val_1.fq.gz,HEK293_DMSO_2hA_3.L3.R1_val_1.fq.gz,HEK293_DMSO_2hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L1.R2_val_2.fq.gz,HEK293_DMSO_2hA_3.L2.R2_val_2.fq.gz,HEK293_DMSO_2hA_3.L3.R2_val_2.fq.gz,HEK293_DMSO_2hA_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_2hA_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 16:36:01 ….. started STAR run May 15 16:36:01 ….. loading genome May 15 16:39:21 ….. started 1st pass mapping May 15 16:40:06 ….. finished 1st pass mapping May 15 16:40:06 ….. inserting junctions into the genome indices May 15 16:41:01 ….. started mapping May 15 16:42:58 ….. finished mapping May 15 16:42:59 ….. started sorting BAM May 15 16:45:09 ….. finished successfully

[2022年 5月 15日日曜日 16:45:14 JST] Indexes HEK293_DMSO_2hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 16:45:19 JST] Converting HEK293_DMSO_2hA_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 16:46:29 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 16:46:29 JST] Run featureCounts to HEK293_DMSO_2hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 16:46:43 JST] Please Check Output Folder: HEK293_OSMI2_2hA_1

[2022年 5月 15日日曜日 16:46:43 JST] Please Check Read1 file(s): HEK293_OSMI2_2hA_1.L1.R1.fastq.gz,HEK293_OSMI2_2hA_1.L2.R1.fastq.gz,HEK293_OSMI2_2hA_1.L3.R1.fastq.gz,HEK293_OSMI2_2hA_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 16:46:43 JST] Please Check Read2 file(s): HEK293_OSMI2_2hA_1.L1.R2.fastq.gz,HEK293_OSMI2_2hA_1.L2.R2.fastq.gz,HEK293_OSMI2_2hA_1.L3.R2.fastq.gz,HEK293_OSMI2_2hA_1.L4.R2.fastq.gz

HEK293_OSMI2_2hA_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 16:50:59 JST] Using Salmon to Map sample 4 with 128 CPU cores: HEK293_OSMI2_2hA_1.L1.R1_val_1.fq.gz HEK293_OSMI2_2hA_1.L2.R1_val_1.fq.gz HEK293_OSMI2_2hA_1.L3.R1_val_1.fq.gz HEK293_OSMI2_2hA_1.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_1.L1.R2_val_2.fq.gz HEK293_OSMI2_2hA_1.L2.R2_val_2.fq.gz HEK293_OSMI2_2hA_1.L3.R2_val_2.fq.gz HEK293_OSMI2_2hA_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 16:53:11 JST] Mapping sample 4 with 128 CPU cores: HEK293_OSMI2_2hA_1.L1.R1_val_1.fq.gz,HEK293_OSMI2_2hA_1.L2.R1_val_1.fq.gz,HEK293_OSMI2_2hA_1.L3.R1_val_1.fq.gz,HEK293_OSMI2_2hA_1.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_1.L1.R2_val_2.fq.gz,HEK293_OSMI2_2hA_1.L2.R2_val_2.fq.gz,HEK293_OSMI2_2hA_1.L3.R2_val_2.fq.gz,HEK293_OSMI2_2hA_1.L4.R2_val_2.fq.gz with prefix:HEK293_OSMI2_2hA_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_OSMI2_2hA_1.L1.R1_val_1.fq.gz,HEK293_OSMI2_2hA_1.L2.R1_val_1.fq.gz,HEK293_OSMI2_2hA_1.L3.R1_val_1.fq.gz,HEK293_OSMI2_2hA_1.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_1.L1.R2_val_2.fq.gz,HEK293_OSMI2_2hA_1.L2.R2_val_2.fq.gz,HEK293_OSMI2_2hA_1.L3.R2_val_2.fq.gz,HEK293_OSMI2_2hA_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_OSMI2_2hA_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 16:53:11 ….. started STAR run May 15 16:53:11 ….. loading genome May 15 16:56:30 ….. started 1st pass mapping May 15 16:57:28 ….. finished 1st pass mapping May 15 16:57:29 ….. inserting junctions into the genome indices May 15 16:58:25 ….. started mapping May 15 17:04:45 ….. finished mapping May 15 17:04:47 ….. started sorting BAM May 15 17:08:29 ….. finished successfully

[2022年 5月 15日日曜日 17:08:32 JST] Indexes HEK293_OSMI2_2hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 17:08:40 JST] Converting HEK293_OSMI2_2hA_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 17:10:20 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 17:10:20 JST] Run featureCounts to HEK293_OSMI2_2hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 17:10:38 JST] Please Check Output Folder: HEK293_OSMI2_2hA_2

[2022年 5月 15日日曜日 17:10:38 JST] Please Check Read1 file(s): HEK293_OSMI2_2hA_2.L1.R1.fastq.gz,HEK293_OSMI2_2hA_2.L2.R1.fastq.gz,HEK293_OSMI2_2hA_2.L3.R1.fastq.gz,HEK293_OSMI2_2hA_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 17:10:38 JST] Please Check Read2 file(s): HEK293_OSMI2_2hA_2.L1.R2.fastq.gz,HEK293_OSMI2_2hA_2.L2.R2.fastq.gz,HEK293_OSMI2_2hA_2.L3.R2.fastq.gz,HEK293_OSMI2_2hA_2.L4.R2.fastq.gz

HEK293_OSMI2_2hA_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 17:16:12 JST] Using Salmon to Map sample 5 with 128 CPU cores: HEK293_OSMI2_2hA_2.L1.R1_val_1.fq.gz HEK293_OSMI2_2hA_2.L2.R1_val_1.fq.gz HEK293_OSMI2_2hA_2.L3.R1_val_1.fq.gz HEK293_OSMI2_2hA_2.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_2.L1.R2_val_2.fq.gz HEK293_OSMI2_2hA_2.L2.R2_val_2.fq.gz HEK293_OSMI2_2hA_2.L3.R2_val_2.fq.gz HEK293_OSMI2_2hA_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 17:18:24 JST] Mapping sample 5 with 128 CPU cores: HEK293_OSMI2_2hA_2.L1.R1_val_1.fq.gz,HEK293_OSMI2_2hA_2.L2.R1_val_1.fq.gz,HEK293_OSMI2_2hA_2.L3.R1_val_1.fq.gz,HEK293_OSMI2_2hA_2.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_2.L1.R2_val_2.fq.gz,HEK293_OSMI2_2hA_2.L2.R2_val_2.fq.gz,HEK293_OSMI2_2hA_2.L3.R2_val_2.fq.gz,HEK293_OSMI2_2hA_2.L4.R2_val_2.fq.gz with prefix:HEK293_OSMI2_2hA_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_OSMI2_2hA_2.L1.R1_val_1.fq.gz,HEK293_OSMI2_2hA_2.L2.R1_val_1.fq.gz,HEK293_OSMI2_2hA_2.L3.R1_val_1.fq.gz,HEK293_OSMI2_2hA_2.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_2.L1.R2_val_2.fq.gz,HEK293_OSMI2_2hA_2.L2.R2_val_2.fq.gz,HEK293_OSMI2_2hA_2.L3.R2_val_2.fq.gz,HEK293_OSMI2_2hA_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_OSMI2_2hA_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 17:18:24 ….. started STAR run May 15 17:18:24 ….. loading genome May 15 17:21:44 ….. started 1st pass mapping May 15 17:23:09 ….. finished 1st pass mapping May 15 17:23:09 ….. inserting junctions into the genome indices May 15 17:24:07 ….. started mapping May 15 17:30:52 ….. finished mapping May 15 17:30:54 ….. started sorting BAM May 15 17:37:27 ….. finished successfully

[2022年 5月 15日日曜日 17:37:31 JST] Indexes HEK293_OSMI2_2hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 17:37:42 JST] Converting HEK293_OSMI2_2hA_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 17:39:54 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 17:39:54 JST] Run featureCounts to HEK293_OSMI2_2hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 17:40:17 JST] Please Check Output Folder: HEK293_OSMI2_2hA_3

[2022年 5月 15日日曜日 17:40:17 JST] Please Check Read1 file(s): HEK293_OSMI2_2hA_3.L1.R1.fastq.gz,HEK293_OSMI2_2hA_3.L2.R1.fastq.gz,HEK293_OSMI2_2hA_3.L3.R1.fastq.gz,HEK293_OSMI2_2hA_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 17:40:17 JST] Please Check Read2 file(s): HEK293_OSMI2_2hA_3.L1.R2.fastq.gz,HEK293_OSMI2_2hA_3.L2.R2.fastq.gz,HEK293_OSMI2_2hA_3.L3.R2.fastq.gz,HEK293_OSMI2_2hA_3.L4.R2.fastq.gz

HEK293_OSMI2_2hA_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_2hA_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 17:43:54 JST] Using Salmon to Map sample 6 with 128 CPU cores: HEK293_OSMI2_2hA_3.L1.R1_val_1.fq.gz HEK293_OSMI2_2hA_3.L2.R1_val_1.fq.gz HEK293_OSMI2_2hA_3.L3.R1_val_1.fq.gz HEK293_OSMI2_2hA_3.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_3.L1.R2_val_2.fq.gz HEK293_OSMI2_2hA_3.L2.R2_val_2.fq.gz HEK293_OSMI2_2hA_3.L3.R2_val_2.fq.gz HEK293_OSMI2_2hA_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 17:45:31 JST] Mapping sample 6 with 128 CPU cores: HEK293_OSMI2_2hA_3.L1.R1_val_1.fq.gz,HEK293_OSMI2_2hA_3.L2.R1_val_1.fq.gz,HEK293_OSMI2_2hA_3.L3.R1_val_1.fq.gz,HEK293_OSMI2_2hA_3.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_3.L1.R2_val_2.fq.gz,HEK293_OSMI2_2hA_3.L2.R2_val_2.fq.gz,HEK293_OSMI2_2hA_3.L3.R2_val_2.fq.gz,HEK293_OSMI2_2hA_3.L4.R2_val_2.fq.gz with prefix:HEK293_OSMI2_2hA_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_OSMI2_2hA_3.L1.R1_val_1.fq.gz,HEK293_OSMI2_2hA_3.L2.R1_val_1.fq.gz,HEK293_OSMI2_2hA_3.L3.R1_val_1.fq.gz,HEK293_OSMI2_2hA_3.L4.R1_val_1.fq.gz HEK293_OSMI2_2hA_3.L1.R2_val_2.fq.gz,HEK293_OSMI2_2hA_3.L2.R2_val_2.fq.gz,HEK293_OSMI2_2hA_3.L3.R2_val_2.fq.gz,HEK293_OSMI2_2hA_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_OSMI2_2hA_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 17:45:31 ….. started STAR run May 15 17:45:31 ….. loading genome May 15 17:48:51 ….. started 1st pass mapping May 15 17:49:47 ….. finished 1st pass mapping May 15 17:49:47 ….. inserting junctions into the genome indices May 15 17:50:43 ….. started mapping May 15 17:54:32 ….. finished mapping May 15 17:54:34 ….. started sorting BAM May 15 17:59:05 ….. finished successfully

[2022年 5月 15日日曜日 17:59:08 JST] Indexes HEK293_OSMI2_2hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 17:59:14 JST] Converting HEK293_OSMI2_2hA_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 18:00:38 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 18:00:38 JST] Run featureCounts to HEK293_OSMI2_2hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:00:54 JST] Please Check Output Folder: HEK293_DMSO_6hA_1

[2022年 5月 15日日曜日 18:00:54 JST] Please Check Read1 file(s): HEK293_DMSO_6hA_1.L1.R1.fastq.gz,HEK293_DMSO_6hA_1.L2.R1.fastq.gz,HEK293_DMSO_6hA_1.L3.R1.fastq.gz,HEK293_DMSO_6hA_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 18:00:54 JST] Please Check Read2 file(s): HEK293_DMSO_6hA_1.L1.R2.fastq.gz,HEK293_DMSO_6hA_1.L2.R2.fastq.gz,HEK293_DMSO_6hA_1.L3.R2.fastq.gz,HEK293_DMSO_6hA_1.L4.R2.fastq.gz

HEK293_DMSO_6hA_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 18:03:49 JST] Using Salmon to Map sample 7 with 128 CPU cores: HEK293_DMSO_6hA_1.L1.R1_val_1.fq.gz HEK293_DMSO_6hA_1.L2.R1_val_1.fq.gz HEK293_DMSO_6hA_1.L3.R1_val_1.fq.gz HEK293_DMSO_6hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_1.L1.R2_val_2.fq.gz HEK293_DMSO_6hA_1.L2.R2_val_2.fq.gz HEK293_DMSO_6hA_1.L3.R2_val_2.fq.gz HEK293_DMSO_6hA_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 18:05:30 JST] Mapping sample 7 with 128 CPU cores: HEK293_DMSO_6hA_1.L1.R1_val_1.fq.gz,HEK293_DMSO_6hA_1.L2.R1_val_1.fq.gz,HEK293_DMSO_6hA_1.L3.R1_val_1.fq.gz,HEK293_DMSO_6hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_1.L1.R2_val_2.fq.gz,HEK293_DMSO_6hA_1.L2.R2_val_2.fq.gz,HEK293_DMSO_6hA_1.L3.R2_val_2.fq.gz,HEK293_DMSO_6hA_1.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_6hA_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_6hA_1.L1.R1_val_1.fq.gz,HEK293_DMSO_6hA_1.L2.R1_val_1.fq.gz,HEK293_DMSO_6hA_1.L3.R1_val_1.fq.gz,HEK293_DMSO_6hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_1.L1.R2_val_2.fq.gz,HEK293_DMSO_6hA_1.L2.R2_val_2.fq.gz,HEK293_DMSO_6hA_1.L3.R2_val_2.fq.gz,HEK293_DMSO_6hA_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_6hA_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 18:05:30 ….. started STAR run May 15 18:05:30 ….. loading genome May 15 18:08:49 ….. started 1st pass mapping May 15 18:09:36 ….. finished 1st pass mapping May 15 18:09:36 ….. inserting junctions into the genome indices May 15 18:10:32 ….. started mapping May 15 18:12:47 ….. finished mapping May 15 18:12:49 ….. started sorting BAM May 15 18:16:19 ….. finished successfully

[2022年 5月 15日日曜日 18:16:21 JST] Indexes HEK293_DMSO_6hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:16:26 JST] Converting HEK293_DMSO_6hA_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 18:17:26 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 18:17:26 JST] Run featureCounts to HEK293_DMSO_6hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:17:39 JST] Please Check Output Folder: HEK293_DMSO_6hA_2

[2022年 5月 15日日曜日 18:17:39 JST] Please Check Read1 file(s): HEK293_DMSO_6hA_2.L1.R1.fastq.gz,HEK293_DMSO_6hA_2.L2.R1.fastq.gz,HEK293_DMSO_6hA_2.L3.R1.fastq.gz,HEK293_DMSO_6hA_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 18:17:39 JST] Please Check Read2 file(s): HEK293_DMSO_6hA_2.L1.R2.fastq.gz,HEK293_DMSO_6hA_2.L2.R2.fastq.gz,HEK293_DMSO_6hA_2.L3.R2.fastq.gz,HEK293_DMSO_6hA_2.L4.R2.fastq.gz

HEK293_DMSO_6hA_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 18:20:58 JST] Using Salmon to Map sample 8 with 128 CPU cores: HEK293_DMSO_6hA_2.L1.R1_val_1.fq.gz HEK293_DMSO_6hA_2.L2.R1_val_1.fq.gz HEK293_DMSO_6hA_2.L3.R1_val_1.fq.gz HEK293_DMSO_6hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_2.L1.R2_val_2.fq.gz HEK293_DMSO_6hA_2.L2.R2_val_2.fq.gz HEK293_DMSO_6hA_2.L3.R2_val_2.fq.gz HEK293_DMSO_6hA_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 18:22:49 JST] Mapping sample 8 with 128 CPU cores: HEK293_DMSO_6hA_2.L1.R1_val_1.fq.gz,HEK293_DMSO_6hA_2.L2.R1_val_1.fq.gz,HEK293_DMSO_6hA_2.L3.R1_val_1.fq.gz,HEK293_DMSO_6hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_2.L1.R2_val_2.fq.gz,HEK293_DMSO_6hA_2.L2.R2_val_2.fq.gz,HEK293_DMSO_6hA_2.L3.R2_val_2.fq.gz,HEK293_DMSO_6hA_2.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_6hA_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_6hA_2.L1.R1_val_1.fq.gz,HEK293_DMSO_6hA_2.L2.R1_val_1.fq.gz,HEK293_DMSO_6hA_2.L3.R1_val_1.fq.gz,HEK293_DMSO_6hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_2.L1.R2_val_2.fq.gz,HEK293_DMSO_6hA_2.L2.R2_val_2.fq.gz,HEK293_DMSO_6hA_2.L3.R2_val_2.fq.gz,HEK293_DMSO_6hA_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_6hA_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 18:22:49 ….. started STAR run May 15 18:22:49 ….. loading genome May 15 18:26:09 ….. started 1st pass mapping May 15 18:27:02 ….. finished 1st pass mapping May 15 18:27:03 ….. inserting junctions into the genome indices May 15 18:27:59 ….. started mapping May 15 18:31:01 ….. finished mapping May 15 18:31:03 ….. started sorting BAM May 15 18:35:41 ….. finished successfully

[2022年 5月 15日日曜日 18:35:44 JST] Indexes HEK293_DMSO_6hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:35:50 JST] Converting HEK293_DMSO_6hA_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 18:37:02 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 18:37:02 JST] Run featureCounts to HEK293_DMSO_6hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:37:17 JST] Please Check Output Folder: HEK293_DMSO_6hA_3

[2022年 5月 15日日曜日 18:37:17 JST] Please Check Read1 file(s): HEK293_DMSO_6hA_3.L1.R1.fastq.gz,HEK293_DMSO_6hA_3.L2.R1.fastq.gz,HEK293_DMSO_6hA_3.L3.R1.fastq.gz,HEK293_DMSO_6hA_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 18:37:17 JST] Please Check Read2 file(s): HEK293_DMSO_6hA_3.L1.R2.fastq.gz,HEK293_DMSO_6hA_3.L2.R2.fastq.gz,HEK293_DMSO_6hA_3.L3.R2.fastq.gz,HEK293_DMSO_6hA_3.L4.R2.fastq.gz

HEK293_DMSO_6hA_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hA_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 18:40:41 JST] Using Salmon to Map sample 9 with 128 CPU cores: HEK293_DMSO_6hA_3.L1.R1_val_1.fq.gz HEK293_DMSO_6hA_3.L2.R1_val_1.fq.gz HEK293_DMSO_6hA_3.L3.R1_val_1.fq.gz HEK293_DMSO_6hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_3.L1.R2_val_2.fq.gz HEK293_DMSO_6hA_3.L2.R2_val_2.fq.gz HEK293_DMSO_6hA_3.L3.R2_val_2.fq.gz HEK293_DMSO_6hA_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 18:42:16 JST] Mapping sample 9 with 128 CPU cores: HEK293_DMSO_6hA_3.L1.R1_val_1.fq.gz,HEK293_DMSO_6hA_3.L2.R1_val_1.fq.gz,HEK293_DMSO_6hA_3.L3.R1_val_1.fq.gz,HEK293_DMSO_6hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_3.L1.R2_val_2.fq.gz,HEK293_DMSO_6hA_3.L2.R2_val_2.fq.gz,HEK293_DMSO_6hA_3.L3.R2_val_2.fq.gz,HEK293_DMSO_6hA_3.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_6hA_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_6hA_3.L1.R1_val_1.fq.gz,HEK293_DMSO_6hA_3.L2.R1_val_1.fq.gz,HEK293_DMSO_6hA_3.L3.R1_val_1.fq.gz,HEK293_DMSO_6hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_6hA_3.L1.R2_val_2.fq.gz,HEK293_DMSO_6hA_3.L2.R2_val_2.fq.gz,HEK293_DMSO_6hA_3.L3.R2_val_2.fq.gz,HEK293_DMSO_6hA_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_6hA_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 18:42:16 ….. started STAR run May 15 18:42:16 ….. loading genome May 15 18:45:35 ….. started 1st pass mapping May 15 18:46:26 ….. finished 1st pass mapping May 15 18:46:27 ….. inserting junctions into the genome indices May 15 18:47:23 ….. started mapping May 15 18:51:18 ….. finished mapping May 15 18:51:20 ….. started sorting BAM May 15 18:56:00 ….. finished successfully

[2022年 5月 15日日曜日 18:56:02 JST] Indexes HEK293_DMSO_6hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:56:08 JST] Converting HEK293_DMSO_6hA_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 18:57:24 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 18:57:24 JST] Run featureCounts to HEK293_DMSO_6hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 18:57:38 JST] Please Check Output Folder: HEK293_OSMI2_6hA_1

[2022年 5月 15日日曜日 18:57:38 JST] Please Check Read1 file(s): HEK293_OSMI2_6hA_1.L1.R1.fastq.gz,HEK293_OSMI2_6hA_1.L2.R1.fastq.gz,HEK293_OSMI2_6hA_1.L3.R1.fastq.gz,HEK293_OSMI2_6hA_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 18:57:38 JST] Please Check Read2 file(s): HEK293_OSMI2_6hA_1.L1.R2.fastq.gz,HEK293_OSMI2_6hA_1.L2.R2.fastq.gz,HEK293_OSMI2_6hA_1.L3.R2.fastq.gz,HEK293_OSMI2_6hA_1.L4.R2.fastq.gz

HEK293_OSMI2_6hA_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 19:00:55 JST] Using Salmon to Map sample 10 with 128 CPU cores: HEK293_OSMI2_6hA_1.L1.R1_val_1.fq.gz HEK293_OSMI2_6hA_1.L2.R1_val_1.fq.gz HEK293_OSMI2_6hA_1.L3.R1_val_1.fq.gz HEK293_OSMI2_6hA_1.L4.R1_val_1.fq.gz HEK293_OSMI2_6hA_1.L1.R2_val_2.fq.gz HEK293_OSMI2_6hA_1.L2.R2_val_2.fq.gz HEK293_OSMI2_6hA_1.L3.R2_val_2.fq.gz HEK293_OSMI2_6hA_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 19:02:33 JST] Mapping sample 10 with 128 CPU cores: HEK293_OSMI2_6hA_1.L1.R1_val_1.fq.gz,HEK293_OSMI2_6hA_1.L2.R1_val_1.fq.gz,HEK293_OSMI2_6hA_1.L3.R1_val_1.fq.gz,HEK293_OSMI2_6hA_1.L4.R1_val_1.fq.gz HEK293_OSMI2_6hA_1.L1.R2_val_2.fq.gz,HEK293_OSMI2_6hA_1.L2.R2_val_2.fq.gz,HEK293_OSMI2_6hA_1.L3.R2_val_2.fq.gz,HEK293_OSMI2_6hA_1.L4.R2_val_2.fq.gz with prefix:HEK293_OSMI2_6hA_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_OSMI2_6hA_1.L1.R1_val_1.fq.gz,HEK293_OSMI2_6hA_1.L2.R1_val_1.fq.gz,HEK293_OSMI2_6hA_1.L3.R1_val_1.fq.gz,HEK293_OSMI2_6hA_1.L4.R1_val_1.fq.gz HEK293_OSMI2_6hA_1.L1.R2_val_2.fq.gz,HEK293_OSMI2_6hA_1.L2.R2_val_2.fq.gz,HEK293_OSMI2_6hA_1.L3.R2_val_2.fq.gz,HEK293_OSMI2_6hA_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_OSMI2_6hA_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 19:02:33 ….. started STAR run May 15 19:02:33 ….. loading genome May 15 19:05:53 ….. started 1st pass mapping May 15 19:06:39 ….. finished 1st pass mapping May 15 19:06:39 ….. inserting junctions into the genome indices May 15 19:07:35 ….. started mapping May 15 19:08:25 ….. finished mapping May 15 19:08:27 ….. started sorting BAM May 15 19:10:18 ….. finished successfully

[2022年 5月 15日日曜日 19:10:21 JST] Indexes HEK293_OSMI2_6hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:10:27 JST] Converting HEK293_OSMI2_6hA_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 19:11:41 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 19:11:41 JST] Run featureCounts to HEK293_OSMI2_6hA_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:11:51 JST] Please Check Output Folder: HEK293_OSMI2_6hA_2

[2022年 5月 15日日曜日 19:11:51 JST] Please Check Read1 file(s): HEK293_OSMI2_6hA_2.L1.R1.fastq.gz [2022年 5月 15日日曜日 19:11:51 JST] Please Check Read2 file(s): HEK293_OSMI2_6hA_2.L1.R2.fastq.gz

HEK293_OSMI2_6hA_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_2.L1.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 19:16:33 JST] Using Salmon to Map sample 11 with 128 CPU cores: HEK293_OSMI2_6hA_2.L1.R1_val_1.fq.gz HEK293_OSMI2_6hA_2.L1.R2_val_2.fq.gz

[2022年 5月 15日日曜日 19:19:14 JST] Mapping sample 11 with 128 CPU cores: HEK293_OSMI2_6hA_2.L1.R1_val_1.fq.gz HEK293_OSMI2_6hA_2.L1.R2_val_2.fq.gz with prefix:HEK293_OSMI2_6hA_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_OSMI2_6hA_2.L1.R1_val_1.fq.gz HEK293_OSMI2_6hA_2.L1.R2_val_2.fq.gz –outFileNamePrefix HEK293_OSMI2_6hA_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 19:19:14 ….. started STAR run May 15 19:19:14 ….. loading genome May 15 19:19:22 ….. started 1st pass mapping May 15 19:20:29 ….. finished 1st pass mapping May 15 19:20:29 ….. inserting junctions into the genome indices May 15 19:21:27 ….. started mapping May 15 19:22:37 ….. finished mapping May 15 19:22:38 ….. started sorting BAM May 15 19:25:21 ….. finished successfully

[2022年 5月 15日日曜日 19:25:26 JST] Indexes HEK293_OSMI2_6hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:25:36 JST] Converting HEK293_OSMI2_6hA_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 19:27:32 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 19:27:32 JST] Run featureCounts to HEK293_OSMI2_6hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:27:46 JST] Please Check Output Folder: HEK293_OSMI2_6hA_3

[2022年 5月 15日日曜日 19:27:46 JST] Please Check Read1 file(s): HEK293_OSMI2_6hA_3.L1.R1.fastq.gz,HEK293_OSMI2_6hA_3.L2.R1.fastq.gz,HEK293_OSMI2_6hA_3.L3.R1.fastq.gz,HEK293_OSMI2_6hA_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 19:27:46 JST] Please Check Read2 file(s): HEK293_OSMI2_6hA_3.L1.R2.fastq.gz,HEK293_OSMI2_6hA_3.L2.R2.fastq.gz,HEK293_OSMI2_6hA_3.L3.R2.fastq.gz,HEK293_OSMI2_6hA_3.L4.R2.fastq.gz

HEK293_OSMI2_6hA_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_OSMI2_6hA_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 19:30:48 JST] Using Salmon to Map sample 12 with 128 CPU cores: HEK293_OSMI2_6hA_3.L1.R1_val_1.fq.gz HEK293_OSMI2_6hA_3.L2.R1_val_1.fq.gz HEK293_OSMI2_6hA_3.L3.R1_val_1.fq.gz HEK293_OSMI2_6hA_3.L4.R1_val_1.fq.gz HEK293_OSMI2_6hA_3.L1.R2_val_2.fq.gz HEK293_OSMI2_6hA_3.L2.R2_val_2.fq.gz HEK293_OSMI2_6hA_3.L3.R2_val_2.fq.gz HEK293_OSMI2_6hA_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 19:32:01 JST] Mapping sample 12 with 128 CPU cores: HEK293_OSMI2_6hA_3.L1.R1_val_1.fq.gz,HEK293_OSMI2_6hA_3.L2.R1_val_1.fq.gz,HEK293_OSMI2_6hA_3.L3.R1_val_1.fq.gz,HEK293_OSMI2_6hA_3.L4.R1_val_1.fq.gz HEK293_OSMI2_6hA_3.L1.R2_val_2.fq.gz,HEK293_OSMI2_6hA_3.L2.R2_val_2.fq.gz,HEK293_OSMI2_6hA_3.L3.R2_val_2.fq.gz,HEK293_OSMI2_6hA_3.L4.R2_val_2.fq.gz with prefix:HEK293_OSMI2_6hA_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_OSMI2_6hA_3.L1.R1_val_1.fq.gz,HEK293_OSMI2_6hA_3.L2.R1_val_1.fq.gz,HEK293_OSMI2_6hA_3.L3.R1_val_1.fq.gz,HEK293_OSMI2_6hA_3.L4.R1_val_1.fq.gz HEK293_OSMI2_6hA_3.L1.R2_val_2.fq.gz,HEK293_OSMI2_6hA_3.L2.R2_val_2.fq.gz,HEK293_OSMI2_6hA_3.L3.R2_val_2.fq.gz,HEK293_OSMI2_6hA_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_OSMI2_6hA_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 19:32:01 ….. started STAR run May 15 19:32:01 ….. loading genome May 15 19:32:08 ….. started 1st pass mapping May 15 19:32:51 ….. finished 1st pass mapping May 15 19:32:52 ….. inserting junctions into the genome indices May 15 19:33:48 ….. started mapping May 15 19:34:34 ….. finished mapping May 15 19:34:35 ….. started sorting BAM May 15 19:36:12 ….. finished successfully

[2022年 5月 15日日曜日 19:36:15 JST] Indexes HEK293_OSMI2_6hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:36:20 JST] Converting HEK293_OSMI2_6hA_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 19:37:23 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 19:37:23 JST] Run featureCounts to HEK293_OSMI2_6hA_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:37:32 JST] Please Check Output Folder: HEK293_DMSO_2hB_1

[2022年 5月 15日日曜日 19:37:32 JST] Please Check Read1 file(s): HEK293_DMSO_2hB_1.L1.R1.fastq.gz,HEK293_DMSO_2hB_1.L2.R1.fastq.gz,HEK293_DMSO_2hB_1.L3.R1.fastq.gz,HEK293_DMSO_2hB_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 19:37:32 JST] Please Check Read2 file(s): HEK293_DMSO_2hB_1.L1.R2.fastq.gz,HEK293_DMSO_2hB_1.L2.R2.fastq.gz,HEK293_DMSO_2hB_1.L3.R2.fastq.gz,HEK293_DMSO_2hB_1.L4.R2.fastq.gz

HEK293_DMSO_2hB_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 19:40:26 JST] Using Salmon to Map sample 13 with 128 CPU cores: HEK293_DMSO_2hB_1.L1.R1_val_1.fq.gz HEK293_DMSO_2hB_1.L2.R1_val_1.fq.gz HEK293_DMSO_2hB_1.L3.R1_val_1.fq.gz HEK293_DMSO_2hB_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_1.L1.R2_val_2.fq.gz HEK293_DMSO_2hB_1.L2.R2_val_2.fq.gz HEK293_DMSO_2hB_1.L3.R2_val_2.fq.gz HEK293_DMSO_2hB_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 19:41:47 JST] Mapping sample 13 with 128 CPU cores: HEK293_DMSO_2hB_1.L1.R1_val_1.fq.gz,HEK293_DMSO_2hB_1.L2.R1_val_1.fq.gz,HEK293_DMSO_2hB_1.L3.R1_val_1.fq.gz,HEK293_DMSO_2hB_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_1.L1.R2_val_2.fq.gz,HEK293_DMSO_2hB_1.L2.R2_val_2.fq.gz,HEK293_DMSO_2hB_1.L3.R2_val_2.fq.gz,HEK293_DMSO_2hB_1.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_2hB_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_2hB_1.L1.R1_val_1.fq.gz,HEK293_DMSO_2hB_1.L2.R1_val_1.fq.gz,HEK293_DMSO_2hB_1.L3.R1_val_1.fq.gz,HEK293_DMSO_2hB_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_1.L1.R2_val_2.fq.gz,HEK293_DMSO_2hB_1.L2.R2_val_2.fq.gz,HEK293_DMSO_2hB_1.L3.R2_val_2.fq.gz,HEK293_DMSO_2hB_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_2hB_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 19:41:47 ….. started STAR run May 15 19:41:47 ….. loading genome May 15 19:41:54 ….. started 1st pass mapping May 15 19:42:33 ….. finished 1st pass mapping May 15 19:42:33 ….. inserting junctions into the genome indices May 15 19:43:29 ….. started mapping May 15 19:44:12 ….. finished mapping May 15 19:44:14 ….. started sorting BAM May 15 19:45:55 ….. finished successfully

[2022年 5月 15日日曜日 19:45:58 JST] Indexes HEK293_DMSO_2hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:46:04 JST] Converting HEK293_DMSO_2hB_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 19:47:16 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 19:47:16 JST] Run featureCounts to HEK293_DMSO_2hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 19:47:26 JST] Please Check Output Folder: HEK293_DMSO_2hB_2

[2022年 5月 15日日曜日 19:47:26 JST] Please Check Read1 file(s): HEK293_DMSO_2hB_2.L1.R1.fastq.gz,HEK293_DMSO_2hB_2.L2.R1.fastq.gz,HEK293_DMSO_2hB_2.L3.R1.fastq.gz,HEK293_DMSO_2hB_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 19:47:26 JST] Please Check Read2 file(s): HEK293_DMSO_2hB_2.L1.R2.fastq.gz,HEK293_DMSO_2hB_2.L2.R2.fastq.gz,HEK293_DMSO_2hB_2.L3.R2.fastq.gz,HEK293_DMSO_2hB_2.L4.R2.fastq.gz

HEK293_DMSO_2hB_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 19:52:31 JST] Using Salmon to Map sample 14 with 128 CPU cores: HEK293_DMSO_2hB_2.L1.R1_val_1.fq.gz HEK293_DMSO_2hB_2.L2.R1_val_1.fq.gz HEK293_DMSO_2hB_2.L3.R1_val_1.fq.gz HEK293_DMSO_2hB_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_2.L1.R2_val_2.fq.gz HEK293_DMSO_2hB_2.L2.R2_val_2.fq.gz HEK293_DMSO_2hB_2.L3.R2_val_2.fq.gz HEK293_DMSO_2hB_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 19:54:03 JST] Mapping sample 14 with 128 CPU cores: HEK293_DMSO_2hB_2.L1.R1_val_1.fq.gz,HEK293_DMSO_2hB_2.L2.R1_val_1.fq.gz,HEK293_DMSO_2hB_2.L3.R1_val_1.fq.gz,HEK293_DMSO_2hB_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_2.L1.R2_val_2.fq.gz,HEK293_DMSO_2hB_2.L2.R2_val_2.fq.gz,HEK293_DMSO_2hB_2.L3.R2_val_2.fq.gz,HEK293_DMSO_2hB_2.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_2hB_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_2hB_2.L1.R1_val_1.fq.gz,HEK293_DMSO_2hB_2.L2.R1_val_1.fq.gz,HEK293_DMSO_2hB_2.L3.R1_val_1.fq.gz,HEK293_DMSO_2hB_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_2.L1.R2_val_2.fq.gz,HEK293_DMSO_2hB_2.L2.R2_val_2.fq.gz,HEK293_DMSO_2hB_2.L3.R2_val_2.fq.gz,HEK293_DMSO_2hB_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_2hB_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 19:54:03 ….. started STAR run May 15 19:54:03 ….. loading genome May 15 19:57:22 ….. started 1st pass mapping May 15 19:58:13 ….. finished 1st pass mapping May 15 19:58:14 ….. inserting junctions into the genome indices May 15 19:59:10 ….. started mapping May 15 20:02:36 ….. finished mapping May 15 20:02:40 ….. started sorting BAM May 15 20:07:28 ….. finished successfully

[2022年 5月 15日日曜日 20:07:32 JST] Indexes HEK293_DMSO_2hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:07:38 JST] Converting HEK293_DMSO_2hB_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 20:08:57 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 20:08:57 JST] Run featureCounts to HEK293_DMSO_2hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:09:11 JST] Please Check Output Folder: HEK293_DMSO_2hB_3

[2022年 5月 15日日曜日 20:09:11 JST] Please Check Read1 file(s): HEK293_DMSO_2hB_3.L1.R1.fastq.gz,HEK293_DMSO_2hB_3.L2.R1.fastq.gz,HEK293_DMSO_2hB_3.L3.R1.fastq.gz,HEK293_DMSO_2hB_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 20:09:11 JST] Please Check Read2 file(s): HEK293_DMSO_2hB_3.L1.R2.fastq.gz,HEK293_DMSO_2hB_3.L2.R2.fastq.gz,HEK293_DMSO_2hB_3.L3.R2.fastq.gz,HEK293_DMSO_2hB_3.L4.R2.fastq.gz

HEK293_DMSO_2hB_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hB_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 20:11:52 JST] Using Salmon to Map sample 15 with 128 CPU cores: HEK293_DMSO_2hB_3.L1.R1_val_1.fq.gz HEK293_DMSO_2hB_3.L2.R1_val_1.fq.gz HEK293_DMSO_2hB_3.L3.R1_val_1.fq.gz HEK293_DMSO_2hB_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_3.L1.R2_val_2.fq.gz HEK293_DMSO_2hB_3.L2.R2_val_2.fq.gz HEK293_DMSO_2hB_3.L3.R2_val_2.fq.gz HEK293_DMSO_2hB_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 20:13:21 JST] Mapping sample 15 with 128 CPU cores: HEK293_DMSO_2hB_3.L1.R1_val_1.fq.gz,HEK293_DMSO_2hB_3.L2.R1_val_1.fq.gz,HEK293_DMSO_2hB_3.L3.R1_val_1.fq.gz,HEK293_DMSO_2hB_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_3.L1.R2_val_2.fq.gz,HEK293_DMSO_2hB_3.L2.R2_val_2.fq.gz,HEK293_DMSO_2hB_3.L3.R2_val_2.fq.gz,HEK293_DMSO_2hB_3.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_2hB_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_2hB_3.L1.R1_val_1.fq.gz,HEK293_DMSO_2hB_3.L2.R1_val_1.fq.gz,HEK293_DMSO_2hB_3.L3.R1_val_1.fq.gz,HEK293_DMSO_2hB_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hB_3.L1.R2_val_2.fq.gz,HEK293_DMSO_2hB_3.L2.R2_val_2.fq.gz,HEK293_DMSO_2hB_3.L3.R2_val_2.fq.gz,HEK293_DMSO_2hB_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_2hB_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 20:13:21 ….. started STAR run May 15 20:13:21 ….. loading genome May 15 20:16:40 ….. started 1st pass mapping May 15 20:17:22 ….. finished 1st pass mapping May 15 20:17:23 ….. inserting junctions into the genome indices May 15 20:18:19 ….. started mapping May 15 20:21:03 ….. finished mapping May 15 20:21:05 ….. started sorting BAM May 15 20:24:47 ….. finished successfully

[2022年 5月 15日日曜日 20:24:49 JST] Indexes HEK293_DMSO_2hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:24:54 JST] Converting HEK293_DMSO_2hB_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 20:25:58 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 20:25:58 JST] Run featureCounts to HEK293_DMSO_2hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:26:11 JST] Please Check Output Folder: HEK293_TMG_2hB_1

[2022年 5月 15日日曜日 20:26:11 JST] Please Check Read1 file(s): HEK293_TMG_2hB_1.L1.R1.fastq.gz,HEK293_TMG_2hB_1.L2.R1.fastq.gz,HEK293_TMG_2hB_1.L3.R1.fastq.gz,HEK293_TMG_2hB_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 20:26:11 JST] Please Check Read2 file(s): HEK293_TMG_2hB_1.L1.R2.fastq.gz,HEK293_TMG_2hB_1.L2.R2.fastq.gz,HEK293_TMG_2hB_1.L3.R2.fastq.gz,HEK293_TMG_2hB_1.L4.R2.fastq.gz

HEK293_TMG_2hB_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 20:28:58 JST] Using Salmon to Map sample 16 with 128 CPU cores: HEK293_TMG_2hB_1.L1.R1_val_1.fq.gz HEK293_TMG_2hB_1.L2.R1_val_1.fq.gz HEK293_TMG_2hB_1.L3.R1_val_1.fq.gz HEK293_TMG_2hB_1.L4.R1_val_1.fq.gz HEK293_TMG_2hB_1.L1.R2_val_2.fq.gz HEK293_TMG_2hB_1.L2.R2_val_2.fq.gz HEK293_TMG_2hB_1.L3.R2_val_2.fq.gz HEK293_TMG_2hB_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 20:30:25 JST] Mapping sample 16 with 128 CPU cores: HEK293_TMG_2hB_1.L1.R1_val_1.fq.gz,HEK293_TMG_2hB_1.L2.R1_val_1.fq.gz,HEK293_TMG_2hB_1.L3.R1_val_1.fq.gz,HEK293_TMG_2hB_1.L4.R1_val_1.fq.gz HEK293_TMG_2hB_1.L1.R2_val_2.fq.gz,HEK293_TMG_2hB_1.L2.R2_val_2.fq.gz,HEK293_TMG_2hB_1.L3.R2_val_2.fq.gz,HEK293_TMG_2hB_1.L4.R2_val_2.fq.gz with prefix:HEK293_TMG_2hB_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_TMG_2hB_1.L1.R1_val_1.fq.gz,HEK293_TMG_2hB_1.L2.R1_val_1.fq.gz,HEK293_TMG_2hB_1.L3.R1_val_1.fq.gz,HEK293_TMG_2hB_1.L4.R1_val_1.fq.gz HEK293_TMG_2hB_1.L1.R2_val_2.fq.gz,HEK293_TMG_2hB_1.L2.R2_val_2.fq.gz,HEK293_TMG_2hB_1.L3.R2_val_2.fq.gz,HEK293_TMG_2hB_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_TMG_2hB_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 20:30:25 ….. started STAR run May 15 20:30:25 ….. loading genome May 15 20:33:44 ….. started 1st pass mapping May 15 20:34:28 ….. finished 1st pass mapping May 15 20:34:29 ….. inserting junctions into the genome indices May 15 20:35:25 ….. started mapping May 15 20:37:22 ….. finished mapping May 15 20:37:25 ….. started sorting BAM May 15 20:40:53 ….. finished successfully

[2022年 5月 15日日曜日 20:40:56 JST] Indexes HEK293_TMG_2hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:41:01 JST] Converting HEK293_TMG_2hB_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 20:42:06 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 20:42:06 JST] Run featureCounts to HEK293_TMG_2hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:42:19 JST] Please Check Output Folder: HEK293_TMG_2hB_2

[2022年 5月 15日日曜日 20:42:19 JST] Please Check Read1 file(s): HEK293_TMG_2hB_2.L1.R1.fastq.gz,HEK293_TMG_2hB_2.L2.R1.fastq.gz,HEK293_TMG_2hB_2.L3.R1.fastq.gz,HEK293_TMG_2hB_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 20:42:19 JST] Please Check Read2 file(s): HEK293_TMG_2hB_2.L1.R2.fastq.gz,HEK293_TMG_2hB_2.L2.R2.fastq.gz,HEK293_TMG_2hB_2.L3.R2.fastq.gz,HEK293_TMG_2hB_2.L4.R2.fastq.gz

HEK293_TMG_2hB_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 20:45:03 JST] Using Salmon to Map sample 17 with 128 CPU cores: HEK293_TMG_2hB_2.L1.R1_val_1.fq.gz HEK293_TMG_2hB_2.L2.R1_val_1.fq.gz HEK293_TMG_2hB_2.L3.R1_val_1.fq.gz HEK293_TMG_2hB_2.L4.R1_val_1.fq.gz HEK293_TMG_2hB_2.L1.R2_val_2.fq.gz HEK293_TMG_2hB_2.L2.R2_val_2.fq.gz HEK293_TMG_2hB_2.L3.R2_val_2.fq.gz HEK293_TMG_2hB_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 20:46:37 JST] Mapping sample 17 with 128 CPU cores: HEK293_TMG_2hB_2.L1.R1_val_1.fq.gz,HEK293_TMG_2hB_2.L2.R1_val_1.fq.gz,HEK293_TMG_2hB_2.L3.R1_val_1.fq.gz,HEK293_TMG_2hB_2.L4.R1_val_1.fq.gz HEK293_TMG_2hB_2.L1.R2_val_2.fq.gz,HEK293_TMG_2hB_2.L2.R2_val_2.fq.gz,HEK293_TMG_2hB_2.L3.R2_val_2.fq.gz,HEK293_TMG_2hB_2.L4.R2_val_2.fq.gz with prefix:HEK293_TMG_2hB_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_TMG_2hB_2.L1.R1_val_1.fq.gz,HEK293_TMG_2hB_2.L2.R1_val_1.fq.gz,HEK293_TMG_2hB_2.L3.R1_val_1.fq.gz,HEK293_TMG_2hB_2.L4.R1_val_1.fq.gz HEK293_TMG_2hB_2.L1.R2_val_2.fq.gz,HEK293_TMG_2hB_2.L2.R2_val_2.fq.gz,HEK293_TMG_2hB_2.L3.R2_val_2.fq.gz,HEK293_TMG_2hB_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_TMG_2hB_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 20:46:37 ….. started STAR run May 15 20:46:37 ….. loading genome May 15 20:49:25 ….. started 1st pass mapping May 15 20:50:04 ….. finished 1st pass mapping May 15 20:50:05 ….. inserting junctions into the genome indices May 15 20:51:01 ….. started mapping May 15 20:51:45 ….. finished mapping May 15 20:51:46 ….. started sorting BAM May 15 20:53:29 ….. finished successfully

[2022年 5月 15日日曜日 20:53:32 JST] Indexes HEK293_TMG_2hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:53:38 JST] Converting HEK293_TMG_2hB_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 20:54:44 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 20:54:44 JST] Run featureCounts to HEK293_TMG_2hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 20:54:53 JST] Please Check Output Folder: HEK293_TMG_2hB_3

[2022年 5月 15日日曜日 20:54:53 JST] Please Check Read1 file(s): HEK293_TMG_2hB_3.L1.R1.fastq.gz,HEK293_TMG_2hB_3.L2.R1.fastq.gz,HEK293_TMG_2hB_3.L3.R1.fastq.gz,HEK293_TMG_2hB_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 20:54:53 JST] Please Check Read2 file(s): HEK293_TMG_2hB_3.L1.R2.fastq.gz,HEK293_TMG_2hB_3.L2.R2.fastq.gz,HEK293_TMG_2hB_3.L3.R2.fastq.gz,HEK293_TMG_2hB_3.L4.R2.fastq.gz

HEK293_TMG_2hB_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_2hB_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 20:57:38 JST] Using Salmon to Map sample 18 with 128 CPU cores: HEK293_TMG_2hB_3.L1.R1_val_1.fq.gz HEK293_TMG_2hB_3.L2.R1_val_1.fq.gz HEK293_TMG_2hB_3.L3.R1_val_1.fq.gz HEK293_TMG_2hB_3.L4.R1_val_1.fq.gz HEK293_TMG_2hB_3.L1.R2_val_2.fq.gz HEK293_TMG_2hB_3.L2.R2_val_2.fq.gz HEK293_TMG_2hB_3.L3.R2_val_2.fq.gz HEK293_TMG_2hB_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 20:59:01 JST] Mapping sample 18 with 128 CPU cores: HEK293_TMG_2hB_3.L1.R1_val_1.fq.gz,HEK293_TMG_2hB_3.L2.R1_val_1.fq.gz,HEK293_TMG_2hB_3.L3.R1_val_1.fq.gz,HEK293_TMG_2hB_3.L4.R1_val_1.fq.gz HEK293_TMG_2hB_3.L1.R2_val_2.fq.gz,HEK293_TMG_2hB_3.L2.R2_val_2.fq.gz,HEK293_TMG_2hB_3.L3.R2_val_2.fq.gz,HEK293_TMG_2hB_3.L4.R2_val_2.fq.gz with prefix:HEK293_TMG_2hB_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_TMG_2hB_3.L1.R1_val_1.fq.gz,HEK293_TMG_2hB_3.L2.R1_val_1.fq.gz,HEK293_TMG_2hB_3.L3.R1_val_1.fq.gz,HEK293_TMG_2hB_3.L4.R1_val_1.fq.gz HEK293_TMG_2hB_3.L1.R2_val_2.fq.gz,HEK293_TMG_2hB_3.L2.R2_val_2.fq.gz,HEK293_TMG_2hB_3.L3.R2_val_2.fq.gz,HEK293_TMG_2hB_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_TMG_2hB_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 20:59:01 ….. started STAR run May 15 20:59:01 ….. loading genome May 15 20:59:11 ….. started 1st pass mapping May 15 20:59:55 ….. finished 1st pass mapping May 15 20:59:55 ….. inserting junctions into the genome indices May 15 21:00:53 ….. started mapping May 15 21:01:38 ….. finished mapping May 15 21:01:40 ….. started sorting BAM May 15 21:03:23 ….. finished successfully

[2022年 5月 15日日曜日 21:03:26 JST] Indexes HEK293_TMG_2hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:03:32 JST] Converting HEK293_TMG_2hB_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 21:04:40 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 21:04:40 JST] Run featureCounts to HEK293_TMG_2hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:04:49 JST] Please Check Output Folder: HEK293_DMSO_6hB_1

[2022年 5月 15日日曜日 21:04:49 JST] Please Check Read1 file(s): HEK293_DMSO_6hB_1.L1.R1.fastq.gz,HEK293_DMSO_6hB_1.L2.R1.fastq.gz,HEK293_DMSO_6hB_1.L3.R1.fastq.gz,HEK293_DMSO_6hB_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 21:04:49 JST] Please Check Read2 file(s): HEK293_DMSO_6hB_1.L1.R2.fastq.gz,HEK293_DMSO_6hB_1.L2.R2.fastq.gz,HEK293_DMSO_6hB_1.L3.R2.fastq.gz,HEK293_DMSO_6hB_1.L4.R2.fastq.gz

HEK293_DMSO_6hB_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 21:07:41 JST] Using Salmon to Map sample 19 with 128 CPU cores: HEK293_DMSO_6hB_1.L1.R1_val_1.fq.gz HEK293_DMSO_6hB_1.L2.R1_val_1.fq.gz HEK293_DMSO_6hB_1.L3.R1_val_1.fq.gz HEK293_DMSO_6hB_1.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_1.L1.R2_val_2.fq.gz HEK293_DMSO_6hB_1.L2.R2_val_2.fq.gz HEK293_DMSO_6hB_1.L3.R2_val_2.fq.gz HEK293_DMSO_6hB_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 21:09:04 JST] Mapping sample 19 with 128 CPU cores: HEK293_DMSO_6hB_1.L1.R1_val_1.fq.gz,HEK293_DMSO_6hB_1.L2.R1_val_1.fq.gz,HEK293_DMSO_6hB_1.L3.R1_val_1.fq.gz,HEK293_DMSO_6hB_1.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_1.L1.R2_val_2.fq.gz,HEK293_DMSO_6hB_1.L2.R2_val_2.fq.gz,HEK293_DMSO_6hB_1.L3.R2_val_2.fq.gz,HEK293_DMSO_6hB_1.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_6hB_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_6hB_1.L1.R1_val_1.fq.gz,HEK293_DMSO_6hB_1.L2.R1_val_1.fq.gz,HEK293_DMSO_6hB_1.L3.R1_val_1.fq.gz,HEK293_DMSO_6hB_1.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_1.L1.R2_val_2.fq.gz,HEK293_DMSO_6hB_1.L2.R2_val_2.fq.gz,HEK293_DMSO_6hB_1.L3.R2_val_2.fq.gz,HEK293_DMSO_6hB_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_6hB_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 21:09:04 ….. started STAR run May 15 21:09:04 ….. loading genome May 15 21:09:11 ….. started 1st pass mapping May 15 21:09:52 ….. finished 1st pass mapping May 15 21:09:53 ….. inserting junctions into the genome indices May 15 21:10:49 ….. started mapping May 15 21:11:33 ….. finished mapping May 15 21:11:34 ….. started sorting BAM May 15 21:13:12 ….. finished successfully

[2022年 5月 15日日曜日 21:13:16 JST] Indexes HEK293_DMSO_6hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:13:21 JST] Converting HEK293_DMSO_6hB_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 21:14:30 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 21:14:30 JST] Run featureCounts to HEK293_DMSO_6hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:14:38 JST] Please Check Output Folder: HEK293_DMSO_6hB_2

[2022年 5月 15日日曜日 21:14:38 JST] Please Check Read1 file(s): HEK293_DMSO_6hB_2.L1.R1.fastq.gz,HEK293_DMSO_6hB_2.L2.R1.fastq.gz,HEK293_DMSO_6hB_2.L3.R1.fastq.gz,HEK293_DMSO_6hB_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 21:14:38 JST] Please Check Read2 file(s): HEK293_DMSO_6hB_2.L1.R2.fastq.gz,HEK293_DMSO_6hB_2.L2.R2.fastq.gz,HEK293_DMSO_6hB_2.L3.R2.fastq.gz,HEK293_DMSO_6hB_2.L4.R2.fastq.gz

HEK293_DMSO_6hB_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 21:17:27 JST] Using Salmon to Map sample 20 with 128 CPU cores: HEK293_DMSO_6hB_2.L1.R1_val_1.fq.gz HEK293_DMSO_6hB_2.L2.R1_val_1.fq.gz HEK293_DMSO_6hB_2.L3.R1_val_1.fq.gz HEK293_DMSO_6hB_2.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_2.L1.R2_val_2.fq.gz HEK293_DMSO_6hB_2.L2.R2_val_2.fq.gz HEK293_DMSO_6hB_2.L3.R2_val_2.fq.gz HEK293_DMSO_6hB_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 21:18:51 JST] Mapping sample 20 with 128 CPU cores: HEK293_DMSO_6hB_2.L1.R1_val_1.fq.gz,HEK293_DMSO_6hB_2.L2.R1_val_1.fq.gz,HEK293_DMSO_6hB_2.L3.R1_val_1.fq.gz,HEK293_DMSO_6hB_2.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_2.L1.R2_val_2.fq.gz,HEK293_DMSO_6hB_2.L2.R2_val_2.fq.gz,HEK293_DMSO_6hB_2.L3.R2_val_2.fq.gz,HEK293_DMSO_6hB_2.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_6hB_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_6hB_2.L1.R1_val_1.fq.gz,HEK293_DMSO_6hB_2.L2.R1_val_1.fq.gz,HEK293_DMSO_6hB_2.L3.R1_val_1.fq.gz,HEK293_DMSO_6hB_2.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_2.L1.R2_val_2.fq.gz,HEK293_DMSO_6hB_2.L2.R2_val_2.fq.gz,HEK293_DMSO_6hB_2.L3.R2_val_2.fq.gz,HEK293_DMSO_6hB_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_6hB_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 21:18:51 ….. started STAR run May 15 21:18:51 ….. loading genome May 15 21:18:58 ….. started 1st pass mapping May 15 21:19:36 ….. finished 1st pass mapping May 15 21:19:37 ….. inserting junctions into the genome indices May 15 21:20:32 ….. started mapping May 15 21:21:12 ….. finished mapping May 15 21:21:14 ….. started sorting BAM May 15 21:22:39 ….. finished successfully

[2022年 5月 15日日曜日 21:22:42 JST] Indexes HEK293_DMSO_6hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:22:47 JST] Converting HEK293_DMSO_6hB_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 21:23:47 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 21:23:47 JST] Run featureCounts to HEK293_DMSO_6hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:23:55 JST] Please Check Output Folder: HEK293_DMSO_6hB_3

[2022年 5月 15日日曜日 21:23:55 JST] Please Check Read1 file(s): HEK293_DMSO_6hB_3.L1.R1.fastq.gz,HEK293_DMSO_6hB_3.L2.R1.fastq.gz,HEK293_DMSO_6hB_3.L3.R1.fastq.gz,HEK293_DMSO_6hB_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 21:23:55 JST] Please Check Read2 file(s): HEK293_DMSO_6hB_3.L1.R2.fastq.gz,HEK293_DMSO_6hB_3.L2.R2.fastq.gz,HEK293_DMSO_6hB_3.L3.R2.fastq.gz,HEK293_DMSO_6hB_3.L4.R2.fastq.gz

HEK293_DMSO_6hB_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_6hB_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 21:26:19 JST] Using Salmon to Map sample 21 with 128 CPU cores: HEK293_DMSO_6hB_3.L1.R1_val_1.fq.gz HEK293_DMSO_6hB_3.L2.R1_val_1.fq.gz HEK293_DMSO_6hB_3.L3.R1_val_1.fq.gz HEK293_DMSO_6hB_3.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_3.L1.R2_val_2.fq.gz HEK293_DMSO_6hB_3.L2.R2_val_2.fq.gz HEK293_DMSO_6hB_3.L3.R2_val_2.fq.gz HEK293_DMSO_6hB_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 21:27:44 JST] Mapping sample 21 with 128 CPU cores: HEK293_DMSO_6hB_3.L1.R1_val_1.fq.gz,HEK293_DMSO_6hB_3.L2.R1_val_1.fq.gz,HEK293_DMSO_6hB_3.L3.R1_val_1.fq.gz,HEK293_DMSO_6hB_3.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_3.L1.R2_val_2.fq.gz,HEK293_DMSO_6hB_3.L2.R2_val_2.fq.gz,HEK293_DMSO_6hB_3.L3.R2_val_2.fq.gz,HEK293_DMSO_6hB_3.L4.R2_val_2.fq.gz with prefix:HEK293_DMSO_6hB_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_DMSO_6hB_3.L1.R1_val_1.fq.gz,HEK293_DMSO_6hB_3.L2.R1_val_1.fq.gz,HEK293_DMSO_6hB_3.L3.R1_val_1.fq.gz,HEK293_DMSO_6hB_3.L4.R1_val_1.fq.gz HEK293_DMSO_6hB_3.L1.R2_val_2.fq.gz,HEK293_DMSO_6hB_3.L2.R2_val_2.fq.gz,HEK293_DMSO_6hB_3.L3.R2_val_2.fq.gz,HEK293_DMSO_6hB_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_DMSO_6hB_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 21:27:44 ….. started STAR run May 15 21:27:44 ….. loading genome May 15 21:27:52 ….. started 1st pass mapping May 15 21:28:27 ….. finished 1st pass mapping May 15 21:28:28 ….. inserting junctions into the genome indices May 15 21:29:23 ….. started mapping May 15 21:30:02 ….. finished mapping May 15 21:30:04 ….. started sorting BAM May 15 21:31:30 ….. finished successfully

[2022年 5月 15日日曜日 21:31:33 JST] Indexes HEK293_DMSO_6hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:31:38 JST] Converting HEK293_DMSO_6hB_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 21:32:38 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 21:32:38 JST] Run featureCounts to HEK293_DMSO_6hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:32:45 JST] Please Check Output Folder: HEK293_TMG_6hB_1

[2022年 5月 15日日曜日 21:32:45 JST] Please Check Read1 file(s): HEK293_TMG_6hB_1.L1.R1.fastq.gz,HEK293_TMG_6hB_1.L2.R1.fastq.gz,HEK293_TMG_6hB_1.L3.R1.fastq.gz,HEK293_TMG_6hB_1.L4.R1.fastq.gz [2022年 5月 15日日曜日 21:32:45 JST] Please Check Read2 file(s): HEK293_TMG_6hB_1.L1.R2.fastq.gz,HEK293_TMG_6hB_1.L2.R2.fastq.gz,HEK293_TMG_6hB_1.L3.R2.fastq.gz,HEK293_TMG_6hB_1.L4.R2.fastq.gz

HEK293_TMG_6hB_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 21:35:44 JST] Using Salmon to Map sample 22 with 128 CPU cores: HEK293_TMG_6hB_1.L1.R1_val_1.fq.gz HEK293_TMG_6hB_1.L2.R1_val_1.fq.gz HEK293_TMG_6hB_1.L3.R1_val_1.fq.gz HEK293_TMG_6hB_1.L4.R1_val_1.fq.gz HEK293_TMG_6hB_1.L1.R2_val_2.fq.gz HEK293_TMG_6hB_1.L2.R2_val_2.fq.gz HEK293_TMG_6hB_1.L3.R2_val_2.fq.gz HEK293_TMG_6hB_1.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 21:37:08 JST] Mapping sample 22 with 128 CPU cores: HEK293_TMG_6hB_1.L1.R1_val_1.fq.gz,HEK293_TMG_6hB_1.L2.R1_val_1.fq.gz,HEK293_TMG_6hB_1.L3.R1_val_1.fq.gz,HEK293_TMG_6hB_1.L4.R1_val_1.fq.gz HEK293_TMG_6hB_1.L1.R2_val_2.fq.gz,HEK293_TMG_6hB_1.L2.R2_val_2.fq.gz,HEK293_TMG_6hB_1.L3.R2_val_2.fq.gz,HEK293_TMG_6hB_1.L4.R2_val_2.fq.gz with prefix:HEK293_TMG_6hB_1.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_TMG_6hB_1.L1.R1_val_1.fq.gz,HEK293_TMG_6hB_1.L2.R1_val_1.fq.gz,HEK293_TMG_6hB_1.L3.R1_val_1.fq.gz,HEK293_TMG_6hB_1.L4.R1_val_1.fq.gz HEK293_TMG_6hB_1.L1.R2_val_2.fq.gz,HEK293_TMG_6hB_1.L2.R2_val_2.fq.gz,HEK293_TMG_6hB_1.L3.R2_val_2.fq.gz,HEK293_TMG_6hB_1.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_TMG_6hB_1.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 21:37:08 ….. started STAR run May 15 21:37:08 ….. loading genome May 15 21:37:15 ….. started 1st pass mapping May 15 21:37:55 ….. finished 1st pass mapping May 15 21:37:56 ….. inserting junctions into the genome indices May 15 21:38:51 ….. started mapping May 15 21:39:34 ….. finished mapping May 15 21:39:35 ….. started sorting BAM May 15 21:41:14 ….. finished successfully

[2022年 5月 15日日曜日 21:41:17 JST] Indexes HEK293_TMG_6hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:41:22 JST] Converting HEK293_TMG_6hB_1.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 21:42:28 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 21:42:28 JST] Run featureCounts to HEK293_TMG_6hB_1.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:42:36 JST] Please Check Output Folder: HEK293_TMG_6hB_2

[2022年 5月 15日日曜日 21:42:36 JST] Please Check Read1 file(s): HEK293_TMG_6hB_2.L1.R1.fastq.gz,HEK293_TMG_6hB_2.L2.R1.fastq.gz,HEK293_TMG_6hB_2.L3.R1.fastq.gz,HEK293_TMG_6hB_2.L4.R1.fastq.gz [2022年 5月 15日日曜日 21:42:36 JST] Please Check Read2 file(s): HEK293_TMG_6hB_2.L1.R2.fastq.gz,HEK293_TMG_6hB_2.L2.R2.fastq.gz,HEK293_TMG_6hB_2.L3.R2.fastq.gz,HEK293_TMG_6hB_2.L4.R2.fastq.gz

HEK293_TMG_6hB_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 21:45:28 JST] Using Salmon to Map sample 23 with 128 CPU cores: HEK293_TMG_6hB_2.L1.R1_val_1.fq.gz HEK293_TMG_6hB_2.L2.R1_val_1.fq.gz HEK293_TMG_6hB_2.L3.R1_val_1.fq.gz HEK293_TMG_6hB_2.L4.R1_val_1.fq.gz HEK293_TMG_6hB_2.L1.R2_val_2.fq.gz HEK293_TMG_6hB_2.L2.R2_val_2.fq.gz HEK293_TMG_6hB_2.L3.R2_val_2.fq.gz HEK293_TMG_6hB_2.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 21:46:57 JST] Mapping sample 23 with 128 CPU cores: HEK293_TMG_6hB_2.L1.R1_val_1.fq.gz,HEK293_TMG_6hB_2.L2.R1_val_1.fq.gz,HEK293_TMG_6hB_2.L3.R1_val_1.fq.gz,HEK293_TMG_6hB_2.L4.R1_val_1.fq.gz HEK293_TMG_6hB_2.L1.R2_val_2.fq.gz,HEK293_TMG_6hB_2.L2.R2_val_2.fq.gz,HEK293_TMG_6hB_2.L3.R2_val_2.fq.gz,HEK293_TMG_6hB_2.L4.R2_val_2.fq.gz with prefix:HEK293_TMG_6hB_2.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_TMG_6hB_2.L1.R1_val_1.fq.gz,HEK293_TMG_6hB_2.L2.R1_val_1.fq.gz,HEK293_TMG_6hB_2.L3.R1_val_1.fq.gz,HEK293_TMG_6hB_2.L4.R1_val_1.fq.gz HEK293_TMG_6hB_2.L1.R2_val_2.fq.gz,HEK293_TMG_6hB_2.L2.R2_val_2.fq.gz,HEK293_TMG_6hB_2.L3.R2_val_2.fq.gz,HEK293_TMG_6hB_2.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_TMG_6hB_2.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 21:46:57 ….. started STAR run May 15 21:46:57 ….. loading genome May 15 21:47:04 ….. started 1st pass mapping May 15 21:47:42 ….. finished 1st pass mapping May 15 21:47:43 ….. inserting junctions into the genome indices May 15 21:48:37 ….. started mapping May 15 21:49:22 ….. finished mapping May 15 21:49:23 ….. started sorting BAM May 15 21:51:48 ….. finished successfully

[2022年 5月 15日日曜日 21:51:53 JST] Indexes HEK293_TMG_6hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:52:02 JST] Converting HEK293_TMG_6hB_2.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 21:53:26 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 21:53:26 JST] Run featureCounts to HEK293_TMG_6hB_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 21:54:10 JST] Please Check Output Folder: HEK293_TMG_6hB_3

[2022年 5月 15日日曜日 21:54:10 JST] Please Check Read1 file(s): HEK293_TMG_6hB_3.L1.R1.fastq.gz,HEK293_TMG_6hB_3.L2.R1.fastq.gz,HEK293_TMG_6hB_3.L3.R1.fastq.gz,HEK293_TMG_6hB_3.L4.R1.fastq.gz [2022年 5月 15日日曜日 21:54:10 JST] Please Check Read2 file(s): HEK293_TMG_6hB_3.L1.R2.fastq.gz,HEK293_TMG_6hB_3.L2.R2.fastq.gz,HEK293_TMG_6hB_3.L3.R2.fastq.gz,HEK293_TMG_6hB_3.L4.R2.fastq.gz

HEK293_TMG_6hB_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_TMG_6hB_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 15日日曜日 21:56:49 JST] Using Salmon to Map sample 24 with 128 CPU cores: HEK293_TMG_6hB_3.L1.R1_val_1.fq.gz HEK293_TMG_6hB_3.L2.R1_val_1.fq.gz HEK293_TMG_6hB_3.L3.R1_val_1.fq.gz HEK293_TMG_6hB_3.L4.R1_val_1.fq.gz HEK293_TMG_6hB_3.L1.R2_val_2.fq.gz HEK293_TMG_6hB_3.L2.R2_val_2.fq.gz HEK293_TMG_6hB_3.L3.R2_val_2.fq.gz HEK293_TMG_6hB_3.L4.R2_val_2.fq.gz

[2022年 5月 15日日曜日 21:57:57 JST] Mapping sample 24 with 128 CPU cores: HEK293_TMG_6hB_3.L1.R1_val_1.fq.gz,HEK293_TMG_6hB_3.L2.R1_val_1.fq.gz,HEK293_TMG_6hB_3.L3.R1_val_1.fq.gz,HEK293_TMG_6hB_3.L4.R1_val_1.fq.gz HEK293_TMG_6hB_3.L1.R2_val_2.fq.gz,HEK293_TMG_6hB_3.L2.R2_val_2.fq.gz,HEK293_TMG_6hB_3.L3.R2_val_2.fq.gz,HEK293_TMG_6hB_3.L4.R2_val_2.fq.gz with prefix:HEK293_TMG_6hB_3.STAR STAR –runMode alignReads –chimSegmentMin 10 –genomeDir ./GenomeIndex_ENSEMBL_STAR_ReadLength74 –readFilesIn HEK293_TMG_6hB_3.L1.R1_val_1.fq.gz,HEK293_TMG_6hB_3.L2.R1_val_1.fq.gz,HEK293_TMG_6hB_3.L3.R1_val_1.fq.gz,HEK293_TMG_6hB_3.L4.R1_val_1.fq.gz HEK293_TMG_6hB_3.L1.R2_val_2.fq.gz,HEK293_TMG_6hB_3.L2.R2_val_2.fq.gz,HEK293_TMG_6hB_3.L3.R2_val_2.fq.gz,HEK293_TMG_6hB_3.L4.R2_val_2.fq.gz –outFileNamePrefix HEK293_TMG_6hB_3.STAR. –alignEndsType Local –outSAMtype BAM SortedByCoordinate –outFilterMultimapNmax 20 –outFilterMismatchNmax 999 –outFilterMismatchNoverLmax 0.04 –alignIntronMin 70 –alignIntronMax 1000000 –alignMatesGapMax 1000000 –alignSJoverhangMin 8 –alignSJDBoverhangMin 1 –outSAMstrandField intronMotif –outFilterType BySJout –twopassMode Basic –outSAMmapqUnique 60 –limitGenomeGenerateRAM 211310333132 –runThreadN 128 –readFilesCommand zcat STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 15 21:57:57 ….. started STAR run May 15 21:57:57 ….. loading genome May 15 21:58:04 ….. started 1st pass mapping May 15 21:58:41 ….. finished 1st pass mapping May 15 21:58:42 ….. inserting junctions into the genome indices May 15 21:59:37 ….. started mapping May 15 22:00:17 ….. finished mapping May 15 22:00:19 ….. started sorting BAM May 15 22:01:43 ….. finished successfully

[2022年 5月 15日日曜日 22:01:46 JST] Indexes HEK293_TMG_6hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 22:01:51 JST] Converting HEK293_TMG_6hB_3.STAR.Aligned.sortedByCoord.out.bam to junc

[2022年 5月 15日日曜日 22:02:51 JST] The Library Strand Type is: 2 [2022年 5月 15日日曜日 22:02:51 JST] Run featureCounts to HEK293_TMG_6hB_3.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 22:02:59 JST] FastQC of STAR bam files Analysis complete for HEK293_DMSO_6hB_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_6hB_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_TMG_6hB_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_2hB_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_TMG_2hB_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_TMG_6hB_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_TMG_2hB_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_6hB_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_TMG_2hB_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_6hA_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_2hB_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_6hA_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_6hA_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_OSMI2_2hA_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_TMG_6hB_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_OSMI2_2hA_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_2hA_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_OSMI2_2hA_2.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_2hA_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_OSMI2_6hA_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_2hA_3.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_DMSO_2hB_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_OSMI2_6hA_1.STAR.Aligned.sortedByCoord.out.bam Analysis complete for HEK293_OSMI2_6hA_2.STAR.Aligned.sortedByCoord.out.bam

[2022年 5月 15日日曜日 22:14:00 JST] Create txt files that will be used to pass the group of inputs to LeafCutter.

[2022年 5月 15日日曜日 22:14:00 JST] Run LeafCutter on 01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA.

[2022年 5月 15日日曜日 22:14:00 JST] Run LeafCutter STEP1.

[2022年 5月 15日日曜日 22:14:15 JST] Run LeafCutter STEP2. Loading counts from ./LeafCutter.Output/01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA_perind_numers.counts.gz Loading metadata from ./01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA.LeafCutter_groups.txt Encoding as HEK293_OSMI2_2hA =0, HEK293_DMSO_2hA =1 Settings: $output_prefix [1] “./LeafCutter.Output/01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA”

$max_cluster_size [1] Inf

$min_samples_per_intron [1] 2

$min_samples_per_group [1] 2

$min_coverage [1] 20

$timeout [1] 30

$num_threads [1] 128

$exon_file [1] “./genome_filtered_exons.txt”

$init [1] “smart”

$seed [1] 12345

$help [1] FALSE

Running differential splicing analysis… Differential splicing summary: statuses 1 <=1 sample with coverage>0 2 <=1 sample with coverage>min_coverage 3 <2 introns used in >=min_samples_per_intron samples 4 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.0258222, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 5 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.0981426, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 6 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[11] = -0.553801, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 7 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[12] = -0.210575, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 8 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[39] = -0.00343691, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 9 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[4] = -0.00318121, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 10 Not enough valid samples 11 Success Freq 1 42 2 3375 3 1648 4 1 5 1 6 1 7 1 8 1 9 1 10 1977 11 20114 Saving results… Loading exons from ./genome_filtered_exons.txt All done, exiting

[2022年 5月 15日日曜日 22:16:59 JST] Run LeafCutter STEP3. Preparing for visualisation Results to be saved in: ./LeafCutter.Output/01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA.LeafCutter.RData Using annotation at: ./LeafCutter.Annotation/ Loading counts from ./LeafCutter.Output/01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA_perind_numers.counts.gz Loading metadata from ./01.HEK293_OSMI2_2hAvsHEK293_DMSO_2hA.LeafCutter_groups.txt Loading exons from ./LeafCutter.Annotation/_all_exons.txt.gz [1] “Annotating junctions” [1] “Preparing results” [1] “converting counts to ratios” [1] “creating PCA”

[2022年 5月 15日日曜日 22:17:34 JST] Run LeafCutter on 02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA.

[2022年 5月 15日日曜日 22:17:34 JST] Run LeafCutter STEP1.

[2022年 5月 15日日曜日 22:17:50 JST] Run LeafCutter STEP2. Loading counts from ./LeafCutter.Output/02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA_perind_numers.counts.gz Loading metadata from ./02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA.LeafCutter_groups.txt Encoding as HEK293_OSMI2_6hA =0, HEK293_DMSO_6hA =1 Settings: $output_prefix [1] “./LeafCutter.Output/02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA”

$max_cluster_size [1] Inf

$min_samples_per_intron [1] 2

$min_samples_per_group [1] 2

$min_coverage [1] 20

$timeout [1] 30

$num_threads [1] 128

$exon_file [1] “./genome_filtered_exons.txt”

$init [1] “smart”

$seed [1] 12345

$help [1] FALSE

Running differential splicing analysis… Differential splicing summary: statuses 1 <=1 sample with coverage>0 2 <=1 sample with coverage>min_coverage 3 <2 introns used in >=min_samples_per_intron samples 4 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.0472713, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 5 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[2] = -0.0656081, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 6 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[3] = -0.00117711, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 7 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[3] = -0.00565288, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 8 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[3] = -0.0156513, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 9 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[4] = -0.00157862, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 10 Not enough valid samples 11 Success Freq 1 92 2 3553 3 1820 4 1 5 1 6 1 7 1 8 1 9 1 10 2107 11 22366 Saving results… Loading exons from ./genome_filtered_exons.txt All done, exiting

[2022年 5月 15日日曜日 22:21:00 JST] Run LeafCutter STEP3. Preparing for visualisation Results to be saved in: ./LeafCutter.Output/02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA.LeafCutter.RData Using annotation at: ./LeafCutter.Annotation/ Loading counts from ./LeafCutter.Output/02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA_perind_numers.counts.gz Loading metadata from ./02.HEK293_OSMI2_6hAvsHEK293_DMSO_6hA.LeafCutter_groups.txt Loading exons from ./LeafCutter.Annotation/_all_exons.txt.gz [1] “Annotating junctions” [1] “Preparing results” [1] “converting counts to ratios” [1] “creating PCA”

[2022年 5月 15日日曜日 22:21:41 JST] Run LeafCutter on 03.HEK293_TMG_2hBvsHEK293_DMSO_2hB.

[2022年 5月 15日日曜日 22:21:41 JST] Run LeafCutter STEP1.

[2022年 5月 15日日曜日 22:21:59 JST] Run LeafCutter STEP2. Loading counts from ./LeafCutter.Output/03.HEK293_TMG_2hBvsHEK293_DMSO_2hB_perind_numers.counts.gz Loading metadata from ./03.HEK293_TMG_2hBvsHEK293_DMSO_2hB.LeafCutter_groups.txt Encoding as HEK293_TMG_2hB =0, HEK293_DMSO_2hB =1 Settings: $output_prefix [1] “./LeafCutter.Output/03.HEK293_TMG_2hBvsHEK293_DMSO_2hB”

$max_cluster_size [1] Inf

$min_samples_per_intron [1] 2

$min_samples_per_group [1] 2

$min_coverage [1] 20

$timeout [1] 30

$num_threads [1] 128

$exon_file [1] “./genome_filtered_exons.txt”

$init [1] “smart”

$seed [1] 12345

$help [1] FALSE

[2022年 5月 15日日曜日 22:26:17 JST] Run LeafCutter STEP3. Preparing for visualisation Results to be saved in: ./LeafCutter.Output/03.HEK293_TMG_2hBvsHEK293_DMSO_2hB.LeafCutter.RData Using annotation at: ./LeafCutter.Annotation/ Loading counts from ./LeafCutter.Output/03.HEK293_TMG_2hBvsHEK293_DMSO_2hB_perind_numers.counts.gz Loading metadata from ./03.HEK293_TMG_2hBvsHEK293_DMSO_2hB.LeafCutter_groups.txt Loading exons from ./LeafCutter.Annotation/_all_exons.txt.gz [1] “Annotating junctions” [1] “Preparing results” [1] “converting counts to ratios” [1] “creating PCA”

[2022年 5月 15日日曜日 22:26:42 JST] Run LeafCutter on 04.HEK293_TMG_6hBvsHEK293_DMSO_6hB.

[2022年 5月 15日日曜日 22:26:42 JST] Run LeafCutter STEP1.

[2022年 5月 15日日曜日 22:26:58 JST] Run LeafCutter STEP2. Loading counts from ./LeafCutter.Output/04.HEK293_TMG_6hBvsHEK293_DMSO_6hB_perind_numers.counts.gz Loading metadata from ./04.HEK293_TMG_6hBvsHEK293_DMSO_6hB.LeafCutter_groups.txt Encoding as HEK293_TMG_6hB =0, HEK293_DMSO_6hB =1 Settings: $output_prefix [1] “./LeafCutter.Output/04.HEK293_TMG_6hBvsHEK293_DMSO_6hB”

$max_cluster_size [1] Inf

$min_samples_per_intron [1] 2

$min_samples_per_group [1] 2

$min_coverage [1] 20

$timeout [1] 30

$num_threads [1] 128

$exon_file [1] “./genome_filtered_exons.txt”

$init [1] “smart”

$seed [1] 12345

$help [1] FALSE

Running differential splicing analysis… Differential splicing summary: statuses Freq 1 <=1 sample with coverage>0 70 2 <=1 sample with coverage>min_coverage 2884 3 <2 introns used in >=min_samples_per_intron samples 1976 4 Not enough valid samples 1221 5 Success 25539 Saving results… Loading exons from ./genome_filtered_exons.txt All done, exiting

[2022年 5月 15日日曜日 22:30:32 JST] Run LeafCutter STEP3. Preparing for visualisation Results to be saved in: ./LeafCutter.Output/04.HEK293_TMG_6hBvsHEK293_DMSO_6hB.LeafCutter.RData Using annotation at: ./LeafCutter.Annotation/ Loading counts from ./LeafCutter.Output/04.HEK293_TMG_6hBvsHEK293_DMSO_6hB_perind_numers.counts.gz Loading metadata from ./04.HEK293_TMG_6hBvsHEK293_DMSO_6hB.LeafCutter_groups.txt Loading exons from ./LeafCutter.Annotation/_all_exons.txt.gz [1] “Annotating junctions” [1] “Preparing results” [1] “converting counts to ratios” [1] “creating PCA”

[2022年 5月 15日日曜日 22:30:55 JST] Run LeafCutter on 05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA.

[2022年 5月 15日日曜日 22:30:55 JST] Run LeafCutter STEP1.

[2022年 5月 15日日曜日 22:31:13 JST] Run LeafCutter STEP2. Loading counts from ./LeafCutter.Output/05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA_perind_numers.counts.gz Loading metadata from ./05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA.LeafCutter_groups.txt Encoding as HEK293_TMG_2hB =0, HEK293_OSMI2_2hA =1 Settings: $output_prefix [1] “./LeafCutter.Output/05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA”

$max_cluster_size [1] Inf

$min_samples_per_intron [1] 2

$min_samples_per_group [1] 2

$min_coverage [1] 20

$timeout [1] 30

$num_threads [1] 128

$exon_file [1] “./genome_filtered_exons.txt”

$init [1] “smart”

$seed [1] 12345

$help [1] FALSE

Running differential splicing analysis… Differential splicing summary: statuses 1 <=1 sample with coverage>0 2 <=1 sample with coverage>min_coverage 3 <2 introns used in >=min_samples_per_intron samples 4 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.00360336, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 5 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[27] = -0.0406006, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 6 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[35] = -0.0318516, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 7 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[5] = -0.0071526, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 8 Not enough valid samples 9 Success Freq 1 53 2 2883 3 2093 4 1 5 1 6 1 7 1 8 5158 9 22939 Saving results… Loading exons from ./genome_filtered_exons.txt All done, exiting

[2022年 5月 15日日曜日 22:35:04 JST] Run LeafCutter STEP3. Preparing for visualisation Results to be saved in: ./LeafCutter.Output/05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA.LeafCutter.RData Using annotation at: ./LeafCutter.Annotation/ Loading counts from ./LeafCutter.Output/05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA_perind_numers.counts.gz Loading metadata from ./05.HEK293_TMG_2hBvsHEK293_OSMI2_2hA.LeafCutter_groups.txt Loading exons from ./LeafCutter.Annotation/_all_exons.txt.gz [1] “Annotating junctions” [1] “Preparing results” [1] “converting counts to ratios” [1] “creating PCA”

[2022年 5月 15日日曜日 22:38:09 JST] Run LeafCutter on 06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA.

[2022年 5月 15日日曜日 22:38:09 JST] Run LeafCutter STEP1.

[2022年 5月 15日日曜日 22:38:26 JST] Run LeafCutter STEP2. Loading counts from ./LeafCutter.Output/06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA_perind_numers.counts.gz Loading metadata from ./06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA.LeafCutter_groups.txt Encoding as HEK293_TMG_6hB =0, HEK293_OSMI2_6hA =1 Settings: $output_prefix [1] “./LeafCutter.Output/06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA”

$max_cluster_size [1] Inf

$min_samples_per_intron [1] 2

$min_samples_per_group [1] 2

$min_coverage [1] 20

$timeout [1] 30

$num_threads [1] 128

$exon_file [1] “./genome_filtered_exons.txt”

$init [1] “smart”

$seed [1] 12345

$help [1] FALSE

Running differential splicing analysis… Differential splicing summary: statuses 1 <=1 sample with coverage>0 2 <=1 sample with coverage>min_coverage 3 <2 introns used in >=min_samples_per_intron samples 4 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.00101995, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 5 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.00218113, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 6 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[1] = -0.0928339, but should be greater than or equal to 0 (in 'model_dm_glm_multi_conc' at line 11) 7 Error in sampler$call_sampler(c(args, dotlist)): Exception: Error transforming variable beta_raw: stan::io::simplex_unconstrain: Vector is not a valid simplex. Vector[15] = -0.048264, but should be greater than or equal to 0 (in ‘model_dm_glm_multi_conc’ at line 11) 8 Not enough valid samples 9 Success Freq 1 109 2 2676 3 2286 4 1 5 1 6 1 7 1 8 5283 9 22297 Saving results… Loading exons from ./genome_filtered_exons.txt All done, exiting

[2022年 5月 15日日曜日 22:41:57 JST] Run LeafCutter STEP3. Preparing for visualisation Results to be saved in: ./LeafCutter.Output/06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA.LeafCutter.RData Using annotation at: ./LeafCutter.Annotation/ Loading counts from ./LeafCutter.Output/06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA_perind_numers.counts.gz Loading metadata from ./06.HEK293_TMG_6hBvsHEK293_OSMI2_6hA.LeafCutter_groups.txt Loading exons from ./LeafCutter.Annotation/_all_exons.txt.gz [1] “Annotating junctions” [1] “Preparing results” [1] “converting counts to ratios” [1] “creating PCA”

[2022年 5月 15日日曜日 22:43:58 JST] Did not find bam list file, now create it

[2022年 5月 15日日曜日 22:43:58 JST] This folder has 24 BAM files. |—-Now start transcript assemblies using stringtie [2022年 5月 15日日曜日 22:43:58 JST] Transcript assemblies on BAM file No.1: ./HEK293_DMSO_2hA_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 22:47:29 JST] Transcript assemblies on BAM file No.2: ./HEK293_DMSO_2hA_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 22:50:51 JST] Transcript assemblies on BAM file No.3: ./HEK293_DMSO_2hA_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 22:54:11 JST] Transcript assemblies on BAM file No.4: ./HEK293_DMSO_2hB_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 23:44:33 JST] Transcript assemblies on BAM file No.5: ./HEK293_DMSO_2hB_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 23:51:57 JST] Transcript assemblies on BAM file No.6: ./HEK293_DMSO_2hB_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 23:56:45 JST] Transcript assemblies on BAM file No.7: ./HEK293_DMSO_6hA_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 15日日曜日 23:59:19 JST] Transcript assemblies on BAM file No.8: ./HEK293_DMSO_6hA_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:02:38 JST] Transcript assemblies on BAM file No.9: ./HEK293_DMSO_6hA_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:06:11 JST] Transcript assemblies on BAM file No.10: ./HEK293_DMSO_6hB_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:08:58 JST] Transcript assemblies on BAM file No.11: ./HEK293_DMSO_6hB_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:11:52 JST] Transcript assemblies on BAM file No.12: ./HEK293_DMSO_6hB_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:14:16 JST] Transcript assemblies on BAM file No.13: ./HEK293_OSMI2_2hA_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:18:16 JST] Transcript assemblies on BAM file No.14: ./HEK293_OSMI2_2hA_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:23:22 JST] Transcript assemblies on BAM file No.15: ./HEK293_OSMI2_2hA_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:29:39 JST] Transcript assemblies on BAM file No.16: ./HEK293_OSMI2_6hA_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:32:50 JST] Transcript assemblies on BAM file No.17: ./HEK293_OSMI2_6hA_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:42:23 JST] Transcript assemblies on BAM file No.18: ./HEK293_OSMI2_6hA_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:45:18 JST] Transcript assemblies on BAM file No.19: ./HEK293_TMG_2hB_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:48:56 JST] Transcript assemblies on BAM file No.20: ./HEK293_TMG_2hB_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 00:59:05 JST] Transcript assemblies on BAM file No.21: ./HEK293_TMG_2hB_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 01:03:12 JST] Transcript assemblies on BAM file No.22: ./HEK293_TMG_6hB_1.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 01:07:44 JST] Transcript assemblies on BAM file No.23: ./HEK293_TMG_6hB_2.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 01:18:12 JST] Transcript assemblies on BAM file No.24: ./HEK293_TMG_6hB_3.STAR.Aligned.sortedByCoord.out.bam.

[2022年 5月 16日月曜日 01:20:35 JST] Merging transcript assemblies using StringTie.

[2022年 5月 16日月曜日 01:22:02 JST] Remove Unstranded Transcripts.

[2022年 5月 16日月曜日 01:22:08 JST] SQANTI3 QC. List of 1 $ axis.text.x:List of 11 ..$ family : NULL ..$ face : NULL ..$ colour : NULL ..$ size : NULL ..$ hjust : num 1 ..$ vjust : NULL ..$ angle : num 60 ..$ lineheight : NULL ..$ margin : NULL ..$ debug : NULL ..$ inherit.blank: logi FALSE ..- attr(, “class”)= chr [1:2] “element_text” “element” - attr(, “class”)= chr [1:2] “theme” “gg” - attr(, “complete”)= logi FALSE - attr(, “validate”)= logi TRUE

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/home/mbb-wzy/miniconda3/envs/SQANTI3.env/bin/pandoc +RTS -K512m -RTS SQANTI3_report.knit.md –to html4 –from markdown+autolink_bare_uris+tex_math_single_backslash –output /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/temp_SQANTI3_report.html –lua-filter /home/mbb-wzy/miniconda3/envs/SQANTI3.env/lib/R/library/rmarkdown/rmarkdown/lua/pagebreak.lua –lua-filter /home/mbb-wzy/miniconda3/envs/SQANTI3.env/lib/R/library/rmarkdown/rmarkdown/lua/latex-div.lua –self-contained –variable bs3=TRUE –standalone –section-divs –table-of-contents –toc-depth 3 –variable toc_float=1 –variable toc_selectors=h1,h2,h3 –variable toc_collapsed=1 –variable toc_smooth_scroll=1 –variable toc_print=1 –template /home/mbb-wzy/miniconda3/envs/SQANTI3.env/lib/R/library/rmarkdown/rmd/h/default.html –no-highlight –variable highlightjs=1 –variable theme=bootstrap –css style.css –mathjax –variable ‘mathjax-url=https://mathjax.rstudio.com/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML’ –include-in-header /tmp/Rtmp1H1RZf/rmarkdown-str714b612ef330.html Write arguments to /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/temp.params.txt… **** Running SQANTI3… **** Parsing provided files…. Reading genome fasta /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genome.fa…. Skipping aligning of sequences because GTF file was provided.

Indels will be not calculated since you ran SQANTI3 without alignment step (SQANTI3 with gtf format as transcriptome input). **** Predicting ORF sequences… **** Parsing Reference Transcriptome…. Splice Junction Coverage files not provided. **** TSS ratio will not be calculated since SR information was not provided **** Performing Classification of Isoforms…. Number of classified isoforms: 291051

[2022年 5月 16日月曜日 01:50:38 JST] Make GenePred flat file for CIRCexplorer2.

[2022年 5月 16日月曜日 01:50:47 JST] Extract transcript sequences using StringTie merged GTF.

[2022年 5月 16日月曜日 01:50:56 JST] Re-Building salmon decoy with StringTie merged GTF **************** *** getDecoy ************* -j = 128 -a = /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/stdout_final.gtf -g = /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genome.fa -t = /mnt/Disk2/02.Metformin_OGlcNAc/01.HEK293_TMG_OSMI2/genomeTx_stdout.fa -o

[2022年 5月 16日月曜日 02:39:43 JST] Re-Building salmon index with StringTie merged GTF Threads = 128 Vertex length = 31 Hash functions = 5 Filter size = 8589934592 Capacity = 2 Files: salmon_index/ref_k31_fixed.fa ——————————————————————————– Round 0, 0:8589934592 Pass Filling Filtering 1 28 21
2 23 1 True junctions count = 2601380 False junctions count = 537988 Hash table size = 3139368 Candidate marks count = 34399780 ——————————————————————————– Reallocating bifurcations time: 1 True marks count: 33493119 Edges construction time: 24 ——————————————————————————– Distinct junctions = 2601380

for info, total work write each : 2.331 total work inram from level 3 : 4.322 total work raw : 25.000 Bitarray 1770115776 bits (100.00 %) (array + ranks ) final hash 0 bits (0.00 %) (nb in final hash 0)

[2022年 5月 16日月曜日 02:42:41 JST] Please Check Output Folder: HEK293_DMSO_2hA_1

[2022年 5月 16日月曜日 02:42:41 JST] Please Check Read1 file(s): HEK293_DMSO_2hA_1.L1.R1.fastq.gz,HEK293_DMSO_2hA_1.L2.R1.fastq.gz,HEK293_DMSO_2hA_1.L3.R1.fastq.gz,HEK293_DMSO_2hA_1.L4.R1.fastq.gz [2022年 5月 16日月曜日 02:42:41 JST] Please Check Read2 file(s): HEK293_DMSO_2hA_1.L1.R2.fastq.gz,HEK293_DMSO_2hA_1.L2.R2.fastq.gz,HEK293_DMSO_2hA_1.L3.R2.fastq.gz,HEK293_DMSO_2hA_1.L4.R2.fastq.gz

HEK293_DMSO_2hA_1.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_1.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 16日月曜日 02:42:41 JST] Using Salmon to Re-Map sample 1 with 128 CPU cores: HEK293_DMSO_2hA_1.L1.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L2.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L3.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_1.L1.R2_val_2.fq.gz HEK293_DMSO_2hA_1.L2.R2_val_2.fq.gz HEK293_DMSO_2hA_1.L3.R2_val_2.fq.gz HEK293_DMSO_2hA_1.L4.R2_val_2.fq.gz

[2022年 5月 16日月曜日 02:44:38 JST] Run CIRCexplorer2 on HEK293_DMSO_2hA_1.STAR.Chimeric.out.junction

[2022年 5月 16日月曜日 02:44:47 JST] Please Check Output Folder: HEK293_DMSO_2hA_2

[2022年 5月 16日月曜日 02:44:47 JST] Please Check Read1 file(s): HEK293_DMSO_2hA_2.L1.R1.fastq.gz,HEK293_DMSO_2hA_2.L2.R1.fastq.gz,HEK293_DMSO_2hA_2.L3.R1.fastq.gz,HEK293_DMSO_2hA_2.L4.R1.fastq.gz [2022年 5月 16日月曜日 02:44:47 JST] Please Check Read2 file(s): HEK293_DMSO_2hA_2.L1.R2.fastq.gz,HEK293_DMSO_2hA_2.L2.R2.fastq.gz,HEK293_DMSO_2hA_2.L3.R2.fastq.gz,HEK293_DMSO_2hA_2.L4.R2.fastq.gz

HEK293_DMSO_2hA_2.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_2.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 16日月曜日 02:44:47 JST] Using Salmon to Re-Map sample 2 with 128 CPU cores: HEK293_DMSO_2hA_2.L1.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L2.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L3.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_2.L1.R2_val_2.fq.gz HEK293_DMSO_2hA_2.L2.R2_val_2.fq.gz HEK293_DMSO_2hA_2.L3.R2_val_2.fq.gz HEK293_DMSO_2hA_2.L4.R2_val_2.fq.gz

[2022年 5月 16日月曜日 02:46:44 JST] Run CIRCexplorer2 on HEK293_DMSO_2hA_2.STAR.Chimeric.out.junction

[2022年 5月 16日月曜日 02:46:53 JST] Please Check Output Folder: HEK293_DMSO_2hA_3

[2022年 5月 16日月曜日 02:46:53 JST] Please Check Read1 file(s): HEK293_DMSO_2hA_3.L1.R1.fastq.gz,HEK293_DMSO_2hA_3.L2.R1.fastq.gz,HEK293_DMSO_2hA_3.L3.R1.fastq.gz,HEK293_DMSO_2hA_3.L4.R1.fastq.gz [2022年 5月 16日月曜日 02:46:53 JST] Please Check Read2 file(s): HEK293_DMSO_2hA_3.L1.R2.fastq.gz,HEK293_DMSO_2hA_3.L2.R2.fastq.gz,HEK293_DMSO_2hA_3.L3.R2.fastq.gz,HEK293_DMSO_2hA_3.L4.R2.fastq.gz

HEK293_DMSO_2hA_3.L1.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L2.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L3.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L4.R1.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L1.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L2.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L3.R2.fastq.gz are encoded with: –Phred33

HEK293_DMSO_2hA_3.L4.R2.fastq.gz are encoded with: –Phred33

[2022年 5月 16日月曜日 02:46:53 JST] Using Salmon to Re-Map sample 3 with 128 CPU cores: HEK293_DMSO_2hA_3.L1.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L2.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L3.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L4.R1_val_1.fq.gz HEK293_DMSO_2hA_3.L1.R2_val_2.fq.gz HEK293_DMSO_2hA_3.L2.R2_val_2.fq.gz HEK293_DMSO_2hA_3.L3.R2_val_2.fq.gz HEK293_DMSO_2hA_3.L4.R2_val_2.fq.gz

[2022年 5月 16日月曜日 02:48:32 JST] Run CIRCexplorer2 on HEK293_DMSO_2hA_3.STAR.Chimeric.out.junction

[2022年 5月 16日月曜日 02:48:41 JST] Please Check Output Folder: HEK293_OSMI2_2hA_1

[2022年 5月 16日月曜日 02:48:41 JST] Please Check Read1 file(s): HEK293_OSMI2_2hA_1.L1.R1.fastq.gz,HEK293_OSMI2_2hA_1.L2.R1.fastq.gz,HEK293_OSMI2_2hA_1.L3.R1.fastq.gz,HEK293_OSMI2_2hA_1.L4.R1.fastq.gz [2022年 5月 16日月曜日 02:48:41 JST] Please Check Read2 file(s): HEK293_OSMI2_2hA_1.L1.R2.fastq.gz,HEK293_OSMI2_2hA_1.L2.R2.fastq.gz,HEK293_OSMI2_2hA_1.L3.R2.fastq.gz,HEK293_OSMI2_2hA_1.L4.R2.fastq.gz