ENSG00000286176

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000661896 ENSG00000286176 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA lncRNA 0.4226862 0.3875835 0.7918669 0.05207137 0.07330036 1.012105 0.033599080 0.0000000 0.19197422 0.00000000 0.19197422 4.3360992 0.049741667 0.0000000 0.20553333 0.20553333 0.94930503 0.04468873   FALSE
ENST00000665800 ENSG00000286176 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA lncRNA 0.4226862 0.3875835 0.7918669 0.05207137 0.07330036 1.012105 0.005971007 0.0000000 0.04776806 0.00000000 0.03131468 2.5302720 0.008079167 0.0000000 0.06463333 0.06463333 0.75384405 0.04468873   FALSE
MSTRG.24523.3 ENSG00000286176 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA   0.4226862 0.3875835 0.7918669 0.05207137 0.07330036 1.012105 0.184846092 0.2366039 0.16739673 0.02431869 0.02373364 -0.4752162 0.485266667 0.6234333 0.21903333 -0.40440000 0.04468873 0.04468873 FALSE TRUE
MSTRG.24523.4 ENSG00000286176 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA   0.4226862 0.3875835 0.7918669 0.05207137 0.07330036 1.012105 0.186180231 0.1509796 0.38472790 0.04767495 0.12278413 1.2939804 0.428833333 0.3765667 0.51076667 0.13420000 0.85829883 0.04468873 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000286176 E001 2.669758 2.331468008 0.586332523 0.76119553 4 6292369 6292559 191 - 0.779 0.000 -14.039
ENSG00000286176 E002 3.025725 2.433674498 0.595329676 0.76725229 4 6292560 6292659 100 - 0.825 0.000 -14.225
ENSG00000286176 E003 4.511963 0.021741289 0.163801197 0.38233037 4 6300031 6300312 282 - 0.636 0.816 0.739
ENSG00000286176 E004 1.518738 0.013696863 0.046025330 0.17011634 4 6300655 6300701 47 - 0.205 0.535 2.009
ENSG00000286176 E005 5.928566 0.046213934 0.321910057 0.56136833 4 6300702 6300956 255 - 0.802 0.898 0.374
ENSG00000286176 E006 1.911343 0.010993753 0.006499644 0.04220184 4 6300957 6301397 441 - 0.205 0.649 2.520
ENSG00000286176 E007 18.034316 0.509472813 0.320912058 0.56050857 4 6301398 6302885 1488 - 1.214 1.345 0.460
ENSG00000286176 E008 7.214592 0.074619593 0.894361720 0.94889038 4 6302886 6303338 453 - 0.928 0.912 -0.060
ENSG00000286176 E009 1.279935 0.296333899 0.316155226   4 6303862 6303914 53 - 0.455 0.222 -1.471
ENSG00000286176 E010 5.077410 0.005451498 0.606913255 0.77482375 4 6303915 6304077 163 - 0.827 0.740 -0.348
ENSG00000286176 E011 13.973506 0.005914401 0.110056196 0.29909382 4 6307267 6308636 1370 - 1.251 1.093 -0.563