ENSG00000280594

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000626994 ENSG00000280594 HEK293_OSMI2_6hA HEK293_TMG_6hB BTG3-AS1 lncRNA lncRNA 1.196099 1.118318 1.634274 0.07684583 0.3615268 0.5432764 0.6805559 0.5341428 1.2346759 0.05862295 0.3633064 1.1937127 0.5018250 0.4840667 0.7498667 0.26580000 0.391317536 0.003085157   FALSE
ENST00000630155 ENSG00000280594 HEK293_OSMI2_6hA HEK293_TMG_6hB BTG3-AS1 lncRNA retained_intron 1.196099 1.118318 1.634274 0.07684583 0.3615268 0.5432764 0.1959909 0.3768222 0.1100858 0.02476658 0.0411654 -1.6876049 0.2081750 0.3432000 0.0610000 -0.28220000 0.003085157 0.003085157   FALSE
ENST00000690626 ENSG00000280594 HEK293_OSMI2_6hA HEK293_TMG_6hB BTG3-AS1 lncRNA lncRNA 1.196099 1.118318 1.634274 0.07684583 0.3615268 0.5432764 0.3195520 0.2073534 0.2895123 0.11981619 0.1731080 0.4625727 0.2899917 0.1727333 0.1891000 0.01636667 0.998491872 0.003085157   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000280594 E001 0.8102147 0.02090195 0.42543904   21 17611708 17611743 36 + 0.323 0.195 -0.958
ENSG00000280594 E002 2.3288507 0.01259594 0.16642481 0.3857745 21 17611744 17611772 29 + 0.634 0.431 -0.959
ENSG00000280594 E003 10.3394859 0.00389711 0.11587066 0.3089332 21 17611773 17612087 315 + 1.118 1.003 -0.418
ENSG00000280594 E004 8.5771683 0.00646813 0.00727240 0.0459423 21 17612088 17613282 1195 + 0.788 1.091 1.139
ENSG00000280594 E005 11.8734744 0.09821145 0.10909012 0.2975827 21 17613283 17614114 832 + 0.943 1.191 0.901
ENSG00000280594 E006 3.8619670 0.09703702 0.06504995 0.2140016 21 17626893 17627049 157 + 0.832 0.519 -1.330
ENSG00000280594 E007 2.4089151 0.01219891 0.15848237 0.3745175 21 17633149 17633199 51 + 0.634 0.431 -0.960