ENSG00000277258

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000620225 ENSG00000277258 HEK293_OSMI2_6hA HEK293_TMG_6hB PCGF2 protein_coding protein_coding 21.47689 32.70599 16.39304 6.862504 0.700525 -0.9960331 7.278860 10.9476015 2.858121 1.8729994 1.47971222 -1.933754 0.3249042 0.343233333 0.1670333 -0.1762000 0.7551359 0.0107865 FALSE TRUE
MSTRG.14245.10 ENSG00000277258 HEK293_OSMI2_6hA HEK293_TMG_6hB PCGF2 protein_coding   21.47689 32.70599 16.39304 6.862504 0.700525 -0.9960331 1.736172 0.1246871 4.442065 0.1246871 1.00956985 5.046791 0.1005875 0.006566667 0.2773667 0.2708000 0.0107865 0.0107865 FALSE FALSE
MSTRG.14245.9 ENSG00000277258 HEK293_OSMI2_6hA HEK293_TMG_6hB PCGF2 protein_coding   21.47689 32.70599 16.39304 6.862504 0.700525 -0.9960331 10.775570 19.0678706 7.044950 5.1963354 0.07522003 -1.435192 0.4958958 0.561400000 0.4315667 -0.1298333 0.4616795 0.0107865 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000277258 E001 0.9289411 0.018583428 0.163874121   17 38733898 38733899 2 - 0.000 0.330 10.403
ENSG00000277258 E002 103.9995651 0.442947500 0.248835069 0.48661846 17 38733900 38734253 354 - 1.792 2.007 0.721
ENSG00000277258 E003 56.5969337 0.227035423 0.177322694 0.40020713 17 38734254 38734279 26 - 1.521 1.750 0.778
ENSG00000277258 E004 199.6615662 0.970646628 0.427124434 0.64880904 17 38734280 38735043 764 - 2.117 2.278 0.537
ENSG00000277258 E005 26.8096718 0.022292863 0.371861624 0.60553136 17 38735044 38735108 65 - 1.288 1.417 0.448
ENSG00000277258 E006 10.2887689 0.003281453 0.222279526 0.45620439 17 38735109 38735111 3 - 0.846 1.044 0.744
ENSG00000277258 E007 23.9612266 0.050501003 0.187472065 0.41370482 17 38735112 38735135 24 - 1.175 1.393 0.766
ENSG00000277258 E008 265.0751061 1.031127523 0.369575575 0.60371132 17 38735136 38735491 356 - 2.184 2.412 0.762
ENSG00000277258 E009 145.3277897 0.517077040 0.206146590 0.43720099 17 38735492 38735564 73 - 1.886 2.160 0.918
ENSG00000277258 E010 105.9719475 0.406555267 0.191519486 0.41879983 17 38735565 38735574 10 - 1.756 2.023 0.900
ENSG00000277258 E011 124.2659530 0.495624527 0.255459777 0.49410537 17 38735575 38735600 26 - 1.860 2.085 0.756
ENSG00000277258 E012 176.7076590 0.001623172 0.870362301 0.93613063 17 38736090 38736170 81 - 2.153 2.210 0.190
ENSG00000277258 E013 193.9902162 0.006381889 0.186771022 0.41275200 17 38738353 38738448 96 - 2.227 2.233 0.017
ENSG00000277258 E014 6.6211476 0.124942605 0.781352802 0.88547808 17 38738449 38738451 3 - 0.812 0.820 0.032
ENSG00000277258 E015 174.1490736 0.032244801 0.076049987 0.23687319 17 38738541 38738595 55 - 2.235 2.164 -0.236
ENSG00000277258 E016 5.0706052 0.349256434 0.129404902 0.33085796 17 38738749 38738752 4 - 0.938 0.633 -1.219
ENSG00000277258 E017 196.5598789 0.046623260 0.123824802 0.32176487 17 38738753 38738861 109 - 2.286 2.217 -0.232
ENSG00000277258 E018 139.2200601 0.049330330 0.110788200 0.30028065 17 38739068 38739118 51 - 2.149 2.063 -0.286
ENSG00000277258 E019 129.9718808 0.037640688 0.061364318 0.20584428 17 38739198 38739253 56 - 2.125 2.032 -0.312
ENSG00000277258 E020 139.7003752 0.031090790 0.036112138 0.14451238 17 38739586 38739682 97 - 2.162 2.061 -0.341
ENSG00000277258 E021 100.8793103 0.019153243 0.005475177 0.03709451 17 38740291 38740442 152 - 2.047 1.911 -0.456
ENSG00000277258 E022 5.1008973 0.159701504 0.358475378 0.59457054 17 38746468 38746628 161 - 0.846 0.695 -0.607
ENSG00000277258 E023 0.6370325 0.091004877 1.000000000   17 38747470 38747552 83 - 0.190 0.196 0.058
ENSG00000277258 E024 8.3083904 0.100695553 0.364461716 0.59943423 17 38747879 38747971 93 - 1.046 0.881 -0.618
ENSG00000277258 E025 10.5718691 0.053493996 0.131779719 0.33460262 17 38747972 38748195 224 - 1.148 0.959 -0.689
ENSG00000277258 E026 5.4883248 0.019764619 0.543536486 0.73159227 17 38748196 38748308 113 - 0.811 0.758 -0.208
ENSG00000277258 E027 0.4458772 0.021768165 0.688866307   17 38748309 38748378 70 - 0.189 0.140 -0.525
ENSG00000277258 E028 11.6311305 0.188811951 0.268328673 0.50797472 17 38749362 38751483 2122 - 0.835 1.104 1.002