ENSG00000274523

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000610322 ENSG00000274523 HEK293_OSMI2_6hA HEK293_TMG_6hB RCC1L protein_coding protein_coding 68.3335 99.53259 37.70565 17.54519 1.043296 -1.40015 58.412661 86.242707 25.726584 17.168602 1.814253 -1.7447491 0.8184667 0.8584 0.6807333 -0.1776667 0.04459517 0.0234463 FALSE TRUE
MSTRG.30067.3 ENSG00000274523 HEK293_OSMI2_6hA HEK293_TMG_6hB RCC1L protein_coding   68.3335 99.53259 37.70565 17.54519 1.043296 -1.40015 6.224567 9.706095 8.458866 1.583238 2.000200 -0.1982078 0.1106417 0.1053 0.2275667 0.1222667 0.44794802 0.0234463 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000274523 E001 53.926369 6.481096e-01 8.171116e-01 9.064707e-01 7 75027122 75028079 958 - 1.747 1.636 -0.375
ENSG00000274523 E002 853.637824 1.937071e-03 7.501841e-04 7.862650e-03 7 75042183 75042670 488 - 2.786 2.874 0.293
ENSG00000274523 E003 989.442924 8.583236e-05 3.197886e-10 2.037803e-08 7 75042671 75042973 303 - 2.850 2.940 0.301
ENSG00000274523 E004 603.869981 2.138458e-04 7.565593e-02 2.361135e-01 7 75042974 75043109 136 - 2.678 2.714 0.120
ENSG00000274523 E005 576.335638 1.947556e-04 6.577869e-02 2.156804e-01 7 75052711 75052796 86 - 2.709 2.681 -0.093
ENSG00000274523 E006 9.691538 3.851573e-03 4.564184e-01 6.706041e-01 7 75055575 75055900 326 - 1.019 0.934 -0.316
ENSG00000274523 E007 776.660715 9.570491e-05 3.257409e-04 3.991795e-03 7 75055901 75056074 174 - 2.852 2.805 -0.157
ENSG00000274523 E008 1.224759 2.078826e-01 2.086727e-02   7 75056517 75056724 208 - 0.650 0.131 -3.296
ENSG00000274523 E009 540.919372 1.773747e-04 3.083660e-02 1.298543e-01 7 75057529 75057616 88 - 2.686 2.652 -0.113
ENSG00000274523 E010 758.643882 9.428416e-04 5.885700e-02 2.001817e-01 7 75058588 75058769 182 - 2.831 2.795 -0.120
ENSG00000274523 E011 466.509567 1.270257e-04 8.644648e-01 9.328479e-01 7 75061207 75061291 85 - 2.588 2.597 0.029
ENSG00000274523 E012 300.131376 2.041595e-04 5.537854e-01 7.389435e-01 7 75063292 75063313 22 - 2.411 2.402 -0.029
ENSG00000274523 E013 334.310033 1.756923e-04 5.642354e-01 7.462861e-01 7 75063314 75063343 30 - 2.457 2.449 -0.026
ENSG00000274523 E014 512.697329 3.128549e-04 3.901964e-02 1.522454e-01 7 75064582 75064648 67 - 2.661 2.626 -0.119
ENSG00000274523 E015 1.601943 1.979348e-02 8.508170e-01 9.254892e-01 7 75064649 75064728 80 - 0.339 0.382 0.252
ENSG00000274523 E016 610.791066 2.239657e-04 1.829628e-04 2.482282e-03 7 75066664 75066792 129 - 2.756 2.696 -0.200
ENSG00000274523 E017 453.817519 1.267307e-04 1.114804e-01 3.014784e-01 7 75070640 75070769 130 - 2.603 2.578 -0.085
ENSG00000274523 E018 11.418683 6.973667e-03 9.970156e-01 1.000000e+00 7 75073180 75073413 234 - 1.019 1.022 0.009
ENSG00000274523 E019 326.724025 3.220838e-03 7.426141e-04 7.801605e-03 7 75073414 75073881 468 - 2.340 2.467 0.424
ENSG00000274523 E020 0.862492 3.521069e-01 9.012424e-01   7 75073993 75074228 236 - 0.200 0.239 0.327