ENSG00000274015

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000620835 ENSG00000274015 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA lncRNA 13.51976 15.31715 8.32038 3.260982 0.5925188 -0.8796357 0.4050001 0.2725235 0.5978844 0.1150924 0.1140621 1.105426 0.04064167 0.01610000 0.07173333 0.05563333 0.01178394 0.01178394    
ENST00000686344 ENSG00000274015 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA lncRNA 13.51976 15.31715 8.32038 3.260982 0.5925188 -0.8796357 11.5039114 13.4092884 4.0808883 3.0421128 2.1350150 -1.713822 0.80991667 0.87123333 0.51940000 -0.35183333 0.78006964 0.01178394    
MSTRG.9697.2 ENSG00000274015 HEK293_OSMI2_6hA HEK293_TMG_6hB lncRNA   13.51976 15.31715 8.32038 3.260982 0.5925188 -0.8796357 1.2977783 1.2400556 3.4306028 0.2404311 2.6519401 1.460669 0.11988750 0.08293333 0.38290000 0.29996667 0.90974228 0.01178394    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000274015 E001 2.196372 0.013972128 0.83108992 0.9144094 14 63642035 63642045 11 + 0.514 0.478 -0.178
ENSG00000274015 E002 2.875531 0.008715082 0.49852405 0.6997748 14 63642046 63642060 15 + 0.644 0.529 -0.516
ENSG00000274015 E003 9.915291 0.007955197 0.79624559 0.8941244 14 63642061 63642072 12 + 0.978 1.004 0.097
ENSG00000274015 E004 29.256325 0.001570110 0.78303976 0.8864502 14 63642073 63642142 70 + 1.450 1.422 -0.096
ENSG00000274015 E005 28.999153 0.001922202 0.59681585 0.7681884 14 63642143 63642152 10 + 1.459 1.413 -0.157
ENSG00000274015 E006 34.429453 0.001251125 0.82163348 0.9089102 14 63642153 63642185 33 + 1.515 1.492 -0.080
ENSG00000274015 E007 30.315769 0.001479044 0.60196637 0.7714535 14 63642186 63642197 12 + 1.476 1.431 -0.152
ENSG00000274015 E008 4.480766 0.312307505 0.04836692 0.1758659 14 63642198 63642696 499 + 0.987 0.519 -1.917
ENSG00000274015 E009 32.892033 0.001492564 0.55174389 0.7374955 14 63651332 63651419 88 + 1.459 1.486 0.095
ENSG00000274015 E010 11.736029 0.004106993 0.02578360 0.1146316 14 63651420 63651426 7 + 0.859 1.116 0.952
ENSG00000274015 E011 10.460622 0.073005347 0.22589676 0.4604310 14 63651427 63651456 30 + 0.855 1.053 0.743
ENSG00000274015 E012 5.020698 0.051197721 0.01469585 0.0769492 14 63651457 63651508 52 + 0.328 0.825 2.330
ENSG00000274015 E013 0.484247 0.269578973 0.02894519   14 63657849 63657886 38 + 0.433 0.000 -12.021
ENSG00000274015 E014 4.671281 0.282216633 0.26986211 0.5098064 14 63664924 63665593 670 + 0.863 0.624 -0.974