ENSG00000273344

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000608317 ENSG00000273344 HEK293_OSMI2_6hA HEK293_TMG_6hB PAXIP1-DT lncRNA lncRNA 4.974281 4.846925 4.314943 0.2989045 0.08778922 -0.167362 4.0691975 4.0884319 3.79725236 0.4137247 0.009972626 -0.1063218 0.82449583 0.83980000 0.8808333 0.04103333 0.86777859 0.01839542   FALSE
MSTRG.30966.3 ENSG00000273344 HEK293_OSMI2_6hA HEK293_TMG_6hB PAXIP1-DT lncRNA   4.974281 4.846925 4.314943 0.2989045 0.08778922 -0.167362 0.3552216 0.3911503 0.02553856 0.0620614 0.025538556 -3.4966861 0.06935417 0.08273333 0.0061000 -0.07663333 0.01839542 0.01839542   FALSE
MSTRG.30966.4 ENSG00000273344 HEK293_OSMI2_6hA HEK293_TMG_6hB PAXIP1-DT lncRNA   4.974281 4.846925 4.314943 0.2989045 0.08778922 -0.167362 0.1973489 0.1974525 0.49215209 0.1086328 0.116177035 1.2753432 0.04592500 0.04230000 0.1131000 0.07080000 0.50263640 0.01839542   FALSE
MSTRG.30966.5 ENSG00000273344 HEK293_OSMI2_6hA HEK293_TMG_6hB PAXIP1-DT lncRNA   4.974281 4.846925 4.314943 0.2989045 0.08778922 -0.167362 0.3390347 0.1319119 0.00000000 0.1319119 0.000000000 -3.8269236 0.05776667 0.02730000 0.0000000 -0.02730000 0.90332100 0.01839542   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000273344 E001 0.3751086 0.027442404 3.658828e-01   7 155003197 155003245 49 + 0.000 0.181 9.285
ENSG00000273344 E002 4.4768486 0.006410043 3.070826e-01 5.476988e-01 7 155003448 155003471 24 + 0.625 0.790 0.683
ENSG00000273344 E003 23.8068849 0.125807163 3.585336e-02 1.438312e-01 7 155003472 155003548 77 + 1.141 1.489 1.218
ENSG00000273344 E004 36.9842219 0.217622742 3.512188e-02 1.419162e-01 7 155003549 155003708 160 + 1.280 1.685 1.394
ENSG00000273344 E005 36.5449292 0.009402052 1.019776e-06 2.890692e-05 7 155003709 155003876 168 + 1.289 1.678 1.338
ENSG00000273344 E006 33.5689683 0.001256077 3.327397e-01 5.714228e-01 7 155003877 155003961 85 + 1.477 1.555 0.270
ENSG00000273344 E007 73.1042718 0.004149723 7.528177e-03 4.715802e-02 7 155003962 155004134 173 + 1.918 1.820 -0.331
ENSG00000273344 E008 57.3878091 0.001350883 1.713243e-03 1.511026e-02 7 155004135 155004340 206 + 1.821 1.701 -0.408
ENSG00000273344 E009 126.9858689 0.016785285 1.166908e-02 6.511184e-02 7 155004341 155005703 1363 + 2.153 2.052 -0.337
ENSG00000273344 E010 0.4514866 0.023268735 3.227182e-01   7 155007560 155007692 133 + 0.256 0.100 -1.641