ENSG00000271254

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000614336 ENSG00000271254 HEK293_OSMI2_6hA HEK293_TMG_6hB protein_coding protein_coding 23.93505 28.38831 23.53244 3.305363 1.088572 -0.270541 12.102872 15.405953 7.641694 1.9094236 0.6298788 -1.0105731 0.5178625 0.5422000 0.3241000 -0.2181 2.119367e-05 2.119367e-05 FALSE TRUE
MSTRG.33771.1 ENSG00000271254 HEK293_OSMI2_6hA HEK293_TMG_6hB protein_coding   23.93505 28.38831 23.53244 3.305363 1.088572 -0.270541 4.547095 4.494878 7.223541 0.4098201 0.3243291 0.6832138 0.1886292 0.1594667 0.3093667 0.1499 2.811404e-03 2.119367e-05 FALSE TRUE
MSTRG.33771.7 ENSG00000271254 HEK293_OSMI2_6hA HEK293_TMG_6hB protein_coding   23.93505 28.38831 23.53244 3.305363 1.088572 -0.270541 3.530719 5.417710 4.032856 0.8127224 0.1331322 -0.4249690 0.1406417 0.1894000 0.1725000 -0.0169 8.776589e-01 2.119367e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000271254 E001 0.0000000       KI270711.1 4612 4674 63 -      
ENSG00000271254 E002 0.0000000       KI270711.1 4675 5258 584 -      
ENSG00000271254 E003 0.1426347 0.0314319579 3.118535e-01   KI270711.1 5259 5511 253 - 0.143 0.000 -8.861
ENSG00000271254 E004 20.4794044 0.1584916987 7.449432e-02 2.338074e-01 KI270711.1 5512 5854 343 - 1.101 1.429 1.156
ENSG00000271254 E005 18.6723015 0.1910409139 2.711502e-01 5.111588e-01 KI270711.1 5855 5890 36 - 1.161 1.356 0.686
ENSG00000271254 E006 18.3450008 0.2677835629 5.666351e-01 7.477305e-01 KI270711.1 5891 5898 8 - 1.235 1.308 0.256
ENSG00000271254 E007 21.6003691 0.2860580351 4.104508e-01 6.364033e-01 KI270711.1 5899 5899 1 - 1.262 1.396 0.467
ENSG00000271254 E008 47.5563136 0.5244823641 3.185438e-01 5.584082e-01 KI270711.1 5900 5934 35 - 1.542 1.750 0.705
ENSG00000271254 E009 84.6777583 0.6922002382 2.858864e-01 5.265222e-01 KI270711.1 5935 6071 137 - 1.748 2.012 0.888
ENSG00000271254 E010 25.9531654 0.2882852926 1.837045e-01 4.087314e-01 KI270711.1 6072 6100 29 - 1.236 1.513 0.961
ENSG00000271254 E011 33.4775542 0.2955971010 9.975660e-02 2.813319e-01 KI270711.1 6101 6370 270 - 1.272 1.643 1.276
ENSG00000271254 E012 24.1933357 0.2199597216 1.350688e-01 3.398230e-01 KI270711.1 6991 7102 112 - 1.192 1.490 1.038
ENSG00000271254 E013 135.2471664 0.0004225216 1.820702e-02 8.970915e-02 KI270711.1 7817 7989 173 - 2.054 2.168 0.381
ENSG00000271254 E014 164.0172153 0.0042607889 1.361769e-01 3.416352e-01 KI270711.1 8604 8655 52 - 2.144 2.250 0.353
ENSG00000271254 E015 167.9171435 0.0058828415 8.358428e-01 9.170839e-01 KI270711.1 9365 9457 93 - 2.186 2.241 0.184
ENSG00000271254 E016 157.3119731 0.0003342778 3.436735e-01 5.813345e-01 KI270711.1 9458 9537 80 - 2.184 2.197 0.043
ENSG00000271254 E017 131.2999747 0.0005163352 5.941877e-01 7.664599e-01 KI270711.1 10140 10191 52 - 2.101 2.123 0.077
ENSG00000271254 E018 112.6417410 0.0004748240 6.286513e-01 7.896505e-01 KI270711.1 10911 11003 93 - 2.035 2.059 0.078
ENSG00000271254 E019 30.5243922 0.0088055330 4.266070e-01 6.485068e-01 KI270711.1 11004 11074 71 - 1.420 1.527 0.367
ENSG00000271254 E020 6.8108007 0.0047186448 8.080984e-01 9.010866e-01 KI270711.1 11075 11077 3 - 0.835 0.901 0.250
ENSG00000271254 E021 6.6276554 0.0051217280 6.136476e-01 7.793377e-01 KI270711.1 11078 11083 6 - 0.882 0.854 -0.107
ENSG00000271254 E022 25.0175232 0.0055912431 4.120746e-02 1.578859e-01 KI270711.1 11707 11758 52 - 1.474 1.359 -0.396
ENSG00000271254 E023 49.2850455 0.0010415675 1.823789e-03 1.586768e-02 KI270711.1 12801 12964 164 - 1.762 1.643 -0.403
ENSG00000271254 E024 2.5190833 0.0088930327 3.568272e-01 5.931803e-01 KI270711.1 14816 14899 84 - 0.615 0.496 -0.547
ENSG00000271254 E025 17.5307150 0.0019141484 5.927688e-02 2.011666e-01 KI270711.1 16871 16922 52 - 1.327 1.210 -0.411
ENSG00000271254 E026 29.8220450 0.0012608650 2.992496e-03 2.335882e-02 KI270711.1 18227 18432 206 - 1.571 1.421 -0.515
ENSG00000271254 E027 30.7860431 0.0011713836 7.416385e-04 7.794249e-03 KI270711.1 18897 18969 73 - 1.593 1.421 -0.590
ENSG00000271254 E028 26.5769525 0.0015423398 2.465896e-04 3.182112e-03 KI270711.1 20007 20221 215 - 1.547 1.339 -0.718
ENSG00000271254 E029 15.8273770 0.0036692030 8.758215e-02 2.592270e-01 KI270711.1 21057 21159 103 - 1.277 1.165 -0.395
ENSG00000271254 E030 56.7076362 0.0021004425 1.415838e-03 1.301303e-02 KI270711.1 22933 23142 210 - 1.820 1.698 -0.411
ENSG00000271254 E031 51.2060563 0.0008191062 6.812116e-01 8.234197e-01 KI270711.1 24492 24703 212 - 1.700 1.718 0.063
ENSG00000271254 E032 0.4873834 0.3865298059 9.132420e-01   KI270711.1 24704 24706 3 - 0.141 0.188 0.497
ENSG00000271254 E033 38.5198409 0.0076224704 5.950799e-01 7.670805e-01 KI270711.1 25167 25239 73 - 1.581 1.588 0.023
ENSG00000271254 E034 61.3145656 0.0076150906 1.101517e-01 2.992356e-01 KI270711.1 26291 26477 187 - 1.816 1.762 -0.181
ENSG00000271254 E035 12.0401811 0.0064133760 9.046133e-01 9.542642e-01 KI270711.1 26478 26505 28 - 1.090 1.115 0.088
ENSG00000271254 E036 11.9858468 0.0313422181 1.646323e-01 3.835502e-01 KI270711.1 27337 27439 103 - 1.187 1.054 -0.481
ENSG00000271254 E037 19.8473944 0.0023646966 1.555749e-07 5.512247e-06 KI270711.1 29279 29488 210 - 1.496 1.124 -1.304
ENSG00000271254 E038 11.3430746 0.0316120524 9.861998e-02 2.793838e-01 KI270711.1 29591 29745 155 - 1.174 0.998 -0.636
ENSG00000271254 E039 1.2311538 0.0136968632 5.175729e-01   KI270711.1 29746 29826 81 - 0.408 0.315 -0.546
ENSG00000271254 E040 1.6421143 0.4902939689 9.872977e-01 9.954986e-01 KI270711.1 32025 32095 71 - 0.456 0.408 -0.255