ENSG00000269439

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000595116 ENSG00000269439 HEK293_OSMI2_6hA HEK293_TMG_6hB PGLS-DT lncRNA lncRNA 1.744516 2.766031 1.507592 0.4132336 0.2292549 -0.8712396 0.3975232 0.3774709 0.56563986 0.002704134 0.12474329 0.5710785 0.2275417 0.1442 0.37586667 0.2316667 0.03129162 0.01969608   FALSE
ENST00000596643 ENSG00000269439 HEK293_OSMI2_6hA HEK293_TMG_6hB PGLS-DT lncRNA lncRNA 1.744516 2.766031 1.507592 0.4132336 0.2292549 -0.8712396 0.2287228 0.6932131 0.03254756 0.216904469 0.03254756 -4.0468135 0.1262333 0.2357 0.02283333 -0.2128667 0.01969608 0.01969608   FALSE
ENST00000598141 ENSG00000269439 HEK293_OSMI2_6hA HEK293_TMG_6hB PGLS-DT lncRNA lncRNA 1.744516 2.766031 1.507592 0.4132336 0.2292549 -0.8712396 0.3758508 0.7165202 0.19355424 0.082189154 0.04989541 -1.8355895 0.2245042 0.2815 0.14176667 -0.1397333 0.57577774 0.01969608   FALSE
ENST00000615939 ENSG00000269439 HEK293_OSMI2_6hA HEK293_TMG_6hB PGLS-DT lncRNA lncRNA 1.744516 2.766031 1.507592 0.4132336 0.2292549 -0.8712396 0.6933423 0.8942617 0.69165185 0.292114746 0.18881700 -0.3659851 0.3903542 0.3026 0.44253333 0.1399333 0.69975829 0.01969608   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000269439 E001 2.595423 0.032119884 0.052919191 0.18652297 19 17488990 17489095 106 - 0.203 0.577 2.217
ENSG00000269439 E002 5.333531 0.032237936 0.103801652 0.28855164 19 17489800 17490120 321 - 0.529 0.773 1.048
ENSG00000269439 E003 9.176125 0.005084342 0.089441116 0.26275184 19 17491076 17491197 122 - 0.803 0.980 0.679
ENSG00000269439 E004 2.769890 0.012279967 0.508828932 0.70702511 19 17491387 17492355 969 - 0.445 0.538 0.458
ENSG00000269439 E005 4.007014 0.007902404 0.976005424 0.98980132 19 17492356 17492392 37 - 0.659 0.636 -0.100
ENSG00000269439 E006 4.359191 0.008156012 0.006234067 0.04088229 19 17503436 17503622 187 - 0.941 0.538 -1.658
ENSG00000269439 E007 13.770353 0.104573542 0.092741107 0.26885580 19 17510100 17510850 751 - 1.283 1.025 -0.923
ENSG00000269439 E008 17.615096 0.002901520 0.495532870 0.69782939 19 17511343 17511889 547 - 1.184 1.205 0.077