ENSG00000268543

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000596296 ENSG00000268543 HEK293_OSMI2_6hA HEK293_TMG_6hB   TEC TEC 3.014194 2.94253 2.167837 0.1985811 0.1639051 -0.4390556 0.7322373 0.4330102 1.2780085 0.4330102 0.2057494 1.5397304 0.25187500 0.15513333 0.5949667 0.43983333 0.21677083 0.01763052   FALSE
MSTRG.17839.1 ENSG00000268543 HEK293_OSMI2_6hA HEK293_TMG_6hB   TEC   3.014194 2.94253 2.167837 0.1985811 0.1639051 -0.4390556 0.7254205 0.7873634 0.0000000 0.3820251 0.0000000 -6.3171655 0.25157500 0.27286667 0.0000000 -0.27286667 0.01763052 0.01763052   FALSE
MSTRG.17839.3 ENSG00000268543 HEK293_OSMI2_6hA HEK293_TMG_6hB   TEC   3.014194 2.94253 2.167837 0.1985811 0.1639051 -0.4390556 1.0238932 1.3086893 0.7272073 0.3875384 0.2861981 -0.8389623 0.34643333 0.43170000 0.3386333 -0.09306667 0.86828794 0.01763052   FALSE
MSTRG.17839.6 ENSG00000268543 HEK293_OSMI2_6hA HEK293_TMG_6hB   TEC   3.014194 2.94253 2.167837 0.1985811 0.1639051 -0.4390556 0.3109362 0.2797853 0.1626216 0.1448734 0.1626216 -0.7473716 0.08317083 0.09076667 0.0664000 -0.02436667 0.82261462 0.01763052   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000268543 E001 7.2246681 0.005496212 0.376848168 0.60971472 19 58418560 58418665 106 + 0.949 0.861 -0.334
ENSG00000268543 E002 10.7903164 0.003043191 0.716003139 0.84561364 19 58418666 58418799 134 + 1.072 1.052 -0.074
ENSG00000268543 E003 5.5188953 0.005681596 0.276185667 0.51619394 19 58418800 58418800 1 + 0.883 0.760 -0.483
ENSG00000268543 E004 6.0304815 0.005164221 0.308316281 0.54895610 19 58418801 58418806 6 + 0.906 0.796 -0.426
ENSG00000268543 E005 11.8149871 0.003493190 0.227658995 0.46256052 19 58418807 58418981 175 + 1.155 1.061 -0.339
ENSG00000268543 E006 9.2265222 0.005071726 0.070894971 0.22650618 19 58419238 58419327 90 + 0.859 1.070 0.785
ENSG00000268543 E007 10.1134200 0.005566678 0.572372158 0.75195294 19 58421199 58421442 244 + 0.988 1.061 0.264
ENSG00000268543 E008 0.8563349 0.018657687 0.422665815   19 58427019 58427055 37 + 0.151 0.302 1.269
ENSG00000268543 E009 3.0743117 0.309854870 0.678573129 0.82183816 19 58428142 58428312 171 + 0.486 0.645 0.727
ENSG00000268543 E010 1.4460641 0.012347689 0.007955269 0.04911611 19 58429387 58429425 39 + 0.000 0.512 11.042
ENSG00000268543 E011 5.4006984 0.010338024 0.762239653 0.87397193 19 58433353 58433424 72 + 0.777 0.828 0.204