ENSG00000256463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000537592 ENSG00000256463 HEK293_OSMI2_6hA HEK293_TMG_6hB SALL3 protein_coding protein_coding 2.425873 4.132386 1.275685 0.6703453 0.1391512 -1.687925 0.1516984 0.1932707 0.01216368 0.05917854 0.01216368 -3.1971329 0.07174583 0.04656667 0.008966667 -0.0376000 0.37261669 0.04056348 FALSE TRUE
ENST00000573324 ENSG00000256463 HEK293_OSMI2_6hA HEK293_TMG_6hB SALL3 protein_coding protein_coding 2.425873 4.132386 1.275685 0.6703453 0.1391512 -1.687925 1.1622819 2.2397635 0.36820015 0.46659058 0.06417991 -2.5725515 0.42797917 0.53353333 0.287000000 -0.2465333 0.04416904 0.04056348 FALSE TRUE
ENST00000575389 ENSG00000256463 HEK293_OSMI2_6hA HEK293_TMG_6hB SALL3 protein_coding protein_coding 2.425873 4.132386 1.275685 0.6703453 0.1391512 -1.687925 1.0730894 1.6263761 0.83598175 0.16015028 0.05118441 -0.9518059 0.48202500 0.40320000 0.663566667 0.2603667 0.04056348 0.04056348 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000256463 E001 2.2348961 0.303578608 0.2820701519 0.522423024 18 78979818 78980274 457 + 0.201 0.503 1.889
ENSG00000256463 E002 4.7308063 0.168693853 0.5074646302 0.705993101 18 78980275 78980356 82 + 0.595 0.720 0.532
ENSG00000256463 E003 0.0000000       18 78985010 78985070 61 +      
ENSG00000256463 E004 1.0116638 0.016349321 0.3127575934   18 78991682 78992073 392 + 0.441 0.232 -1.322
ENSG00000256463 E005 84.1269452 0.009631084 0.2740121145 0.513967166 18 78992074 78994667 2594 + 1.929 1.835 -0.317
ENSG00000256463 E006 13.1856645 0.002538401 0.3475102131 0.584934652 18 78994668 78994797 130 + 1.041 1.097 0.201
ENSG00000256463 E007 11.2348232 0.003722240 0.1409688623 0.348989234 18 78994798 78994905 108 + 0.906 1.038 0.491
ENSG00000256463 E008 0.5171231 0.967399336 0.8407950338   18 78994906 78995121 216 + 0.000 0.190 9.279
ENSG00000256463 E009 21.6681954 0.002545741 0.0163471153 0.083063322 18 78995122 78995462 341 + 1.144 1.315 0.603
ENSG00000256463 E010 104.1125625 0.017392405 0.0465442060 0.171407310 18 78996891 78998969 2079 + 2.052 1.908 -0.483
ENSG00000256463 E011 21.2858768 0.003690500 0.0005010598 0.005685653 18 79002289 79002677 389 + 1.042 1.335 1.043