ENSG00000253549

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000517697 ENSG00000253549 HEK293_OSMI2_6hA HEK293_TMG_6hB   lncRNA lncRNA 0.7336524 0.7763593 0.6606152 0.1073992 0.04160912 -0.2297035 0.04493966 0.02254420 0.10337541 0.02254420 0.10337541 1.800636 0.05921667 0.02276667 0.13896667 0.11620000 0.96683495 0.02675494 FALSE FALSE
ENST00000521761 ENSG00000253549 HEK293_OSMI2_6hA HEK293_TMG_6hB   lncRNA lncRNA 0.7336524 0.7763593 0.6606152 0.1073992 0.04160912 -0.2297035 0.01670657 0.00000000 0.09774896 0.00000000 0.05048366 3.429602 0.02174583 0.00000000 0.14643333 0.14643333 0.26893254 0.02675494   FALSE
ENST00000524052 ENSG00000253549 HEK293_OSMI2_6hA HEK293_TMG_6hB   lncRNA lncRNA 0.7336524 0.7763593 0.6606152 0.1073992 0.04160912 -0.2297035 0.55179886 0.68611402 0.31996632 0.08630524 0.02019254 -1.077005 0.73704167 0.88860000 0.48890000 -0.39970000 0.02675494 0.02675494 TRUE FALSE
ENST00000660227 ENSG00000253549 HEK293_OSMI2_6hA HEK293_TMG_6hB   lncRNA lncRNA 0.7336524 0.7763593 0.6606152 0.1073992 0.04160912 -0.2297035 0.05469188 0.03801305 0.11350255 0.03801305 0.07219893 1.363042 0.07660417 0.05863333 0.18356667 0.12493333 0.73536230 0.02675494 TRUE FALSE
ENST00000666645 ENSG00000253549 HEK293_OSMI2_6hA HEK293_TMG_6hB   lncRNA lncRNA 0.7336524 0.7763593 0.6606152 0.1073992 0.04160912 -0.2297035 0.04226435 0.02968800 0.02602194 0.02968800 0.02602194 -0.139827 0.05607500 0.03000000 0.04213333 0.01213333 1.00000000 0.02675494   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000253549 E001 1.5275719 0.034781723 2.335711e-02 1.070601e-01 8 85440596 85440911 316 - 0.597 0.175 -2.575
ENSG00000253549 E002 4.8359144 0.008653605 2.865046e-09 1.517152e-07 8 85440912 85441892 981 - 1.092 0.241 -3.933
ENSG00000253549 E003 0.6434498 0.020433569 1.269587e-02   8 85441893 85442022 130 - 0.428 0.000 -13.111
ENSG00000253549 E004 0.6847684 0.019748815 1.536025e-01   8 85442023 85442104 82 - 0.354 0.096 -2.343
ENSG00000253549 E005 0.8216059 0.250471858 9.538115e-01   8 85442105 85442237 133 - 0.262 0.245 -0.129
ENSG00000253549 E006 0.1308682 0.032649190 6.944850e-01   8 85444007 85444120 114 - 0.000 0.096 9.067
ENSG00000253549 E007 2.7610431 0.032244815 3.529881e-01 5.900089e-01 8 85456485 85456574 90 - 0.491 0.622 0.603
ENSG00000253549 E008 10.0329084 0.003413441 1.405423e-03 1.293972e-02 8 85456575 85456863 289 - 0.838 1.118 1.045
ENSG00000253549 E009 7.4652565 0.005585513 1.742117e-01 3.961378e-01 8 85462736 85462801 66 - 0.837 0.951 0.433
ENSG00000253549 E010 0.4231520 0.027050377 8.459860e-01   8 85462802 85462931 130 - 0.152 0.174 0.234
ENSG00000253549 E011 3.8484599 0.134272207 8.720092e-01 9.370059e-01 8 85462932 85463177 246 - 0.713 0.666 -0.197
ENSG00000253549 E012 8.3769072 0.007362031 2.394371e-01 4.759567e-01 8 85463178 85463801 624 - 0.910 0.996 0.320
ENSG00000253549 E013 1.6130893 0.012034484 1.883454e-01 4.148344e-01 8 85464228 85464420 193 - 0.265 0.474 1.238
ENSG00000253549 E014 0.4929928 0.024176504 3.497384e-01   8 85464582 85464915 334 - 0.265 0.096 -1.759