ENSG00000251322

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000445220 ENSG00000251322 HEK293_OSMI2_6hA HEK293_TMG_6hB SHANK3 protein_coding protein_coding 9.079987 12.96688 7.042249 1.634012 0.3986008 -0.8797886 2.5445402 3.971513 0.1373941 0.4224930 0.1373941 -4.755565811 0.2667875 0.3080000 0.01756667 -0.290433333 0.01486026 0.01486026 FALSE TRUE
MSTRG.22385.16 ENSG00000251322 HEK293_OSMI2_6hA HEK293_TMG_6hB SHANK3 protein_coding   9.079987 12.96688 7.042249 1.634012 0.3986008 -0.8797886 2.7004244 3.410697 3.3971054 0.8875854 0.6870909 -0.005743681 0.3044333 0.2535333 0.47853333 0.225000000 0.32835002 0.01486026 FALSE TRUE
MSTRG.22385.5 ENSG00000251322 HEK293_OSMI2_6hA HEK293_TMG_6hB SHANK3 protein_coding   9.079987 12.96688 7.042249 1.634012 0.3986008 -0.8797886 1.9950467 2.666037 1.4155478 0.2564376 0.7279669 -0.908581382 0.2245542 0.2076333 0.20160000 -0.006033333 0.93873287 0.01486026 FALSE TRUE
MSTRG.22385.9 ENSG00000251322 HEK293_OSMI2_6hA HEK293_TMG_6hB SHANK3 protein_coding   9.079987 12.96688 7.042249 1.634012 0.3986008 -0.8797886 0.7161981 1.757684 0.5853583 0.0966513 0.3174299 -1.570030067 0.0746500 0.1384000 0.08193333 -0.056466667 0.76544460 0.01486026 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000251322 E001 0.3032425 0.024441170 1.000000e+00   22 50674415 50674704 290 + 0.000 0.144 20.315
ENSG00000251322 E002 0.0000000       22 50674881 50674891 11 +      
ENSG00000251322 E003 2.4891853 0.009651563 3.498096e-01 0.5870768039 22 50674892 50675013 122 + 0.313 0.552 1.279
ENSG00000251322 E004 2.2047239 0.040737565 5.084243e-01 0.7067897109 22 50675014 50675021 8 + 0.313 0.502 1.043
ENSG00000251322 E005 2.7107006 0.028138744 3.006752e-01 0.5411415141 22 50675022 50675047 26 + 0.313 0.576 1.390
ENSG00000251322 E006 2.6803407 0.009203593 2.916088e-01 0.5322628482 22 50675048 50675051 4 + 0.313 0.575 1.386
ENSG00000251322 E007 3.1200609 0.008049069 4.479958e-01 0.6642443172 22 50675052 50675064 13 + 0.413 0.618 0.991
ENSG00000251322 E008 10.9107221 0.003446335 6.941908e-01 0.8318266757 22 50675065 50675162 98 + 0.924 1.058 0.498
ENSG00000251322 E009 20.2967881 0.001887375 4.324211e-02 0.1631817367 22 50675163 50675251 89 + 1.335 1.258 -0.266
ENSG00000251322 E010 21.9554724 0.003306344 1.820384e-03 0.0158421204 22 50676622 50676693 72 + 1.421 1.263 -0.550
ENSG00000251322 E011 24.7132002 0.001744883 4.753430e-04 0.0054524939 22 50678585 50678693 109 + 1.478 1.312 -0.576
ENSG00000251322 E012 28.1405431 0.015145002 1.269865e-01 0.3269885640 22 50678769 50678920 152 + 1.444 1.403 -0.144
ENSG00000251322 E013 37.3056115 0.049178622 2.147900e-01 0.4472709777 22 50679019 50679186 168 + 1.553 1.525 -0.095
ENSG00000251322 E014 27.8322248 0.026113558 2.075523e-01 0.4388708046 22 50679312 50679428 117 + 1.422 1.402 -0.068
ENSG00000251322 E015 21.1928365 0.033992249 1.167405e-01 0.3103634471 22 50683340 50683417 78 + 1.346 1.274 -0.250
ENSG00000251322 E016 20.0099887 0.002203022 5.975069e-02 0.2022141476 22 50684585 50684651 67 + 1.324 1.258 -0.230
ENSG00000251322 E017 0.5530924 0.027886131 6.646376e-01   22 50688766 50694068 5303 + 0.184 0.145 -0.421
ENSG00000251322 E018 26.7147580 0.024472094 2.897205e-02 0.1242768627 22 50694775 50695048 274 + 1.470 1.361 -0.378
ENSG00000251322 E019 0.6412370 0.222252641 3.612915e-03   22 50696023 50696044 22 + 0.493 0.000 -24.196
ENSG00000251322 E020 1.2482695 0.429139787 6.015096e-01   22 50697564 50697564 1 + 0.404 0.299 -0.629
ENSG00000251322 E021 1.9569805 0.112827077 9.656028e-01 0.9847060877 22 50697565 50697581 17 + 0.410 0.446 0.195
ENSG00000251322 E022 1.3573224 0.013453568 4.645182e-01   22 50697582 50697584 3 + 0.184 0.377 1.387
ENSG00000251322 E023 28.1647776 0.155392112 6.380546e-01 0.7957521722 22 50697585 50697715 131 + 1.404 1.415 0.039
ENSG00000251322 E024 0.6479912 0.206740043 4.234219e-01   22 50697999 50698122 124 + 0.000 0.253 21.316
ENSG00000251322 E025 0.5171231 0.863981662 1.000000e+00   22 50698123 50698143 21 + 0.000 0.207 20.947
ENSG00000251322 E026 0.9927399 0.502505552 4.034979e-01   22 50698144 50698342 199 + 0.000 0.342 21.916
ENSG00000251322 E027 0.6536007 0.051316128 9.409021e-01   22 50698343 50698689 347 + 0.185 0.202 0.158
ENSG00000251322 E028 64.4011788 0.099245611 3.498261e-01 0.5870889780 22 50698690 50698803 114 + 1.779 1.757 -0.075
ENSG00000251322 E029 79.7521021 0.078005382 1.309289e-01 0.3332643738 22 50703860 50703935 76 + 1.910 1.832 -0.263
ENSG00000251322 E030 82.0850150 0.054950167 1.677770e-02 0.0846451057 22 50704166 50704248 83 + 1.971 1.822 -0.502
ENSG00000251322 E031 88.3752351 0.038342817 4.786546e-03 0.0334595204 22 50704738 50704862 125 + 2.004 1.855 -0.501
ENSG00000251322 E032 80.7166655 0.017494815 4.857980e-02 0.1763454240 22 50704964 50705096 133 + 1.898 1.849 -0.165
ENSG00000251322 E033 6.5480253 0.126672970 2.105369e-01 0.4424671924 22 50705376 50705495 120 + 0.925 0.790 -0.520
ENSG00000251322 E034 9.1593662 0.089149259 4.136652e-02 0.1582841778 22 50705496 50706071 576 + 1.121 0.889 -0.854
ENSG00000251322 E035 47.2616666 0.016820955 4.275850e-01 0.6491623126 22 50706072 50706152 81 + 1.630 1.641 0.038
ENSG00000251322 E036 0.5227326 0.432014746 6.148589e-01   22 50711615 50711638 24 + 0.186 0.146 -0.418
ENSG00000251322 E037 62.7900887 0.313876055 4.767757e-01 0.6848475823 22 50714917 50715047 131 + 1.669 1.780 0.378
ENSG00000251322 E038 45.2676840 0.103302714 7.734715e-01 0.8807793692 22 50715669 50715753 85 + 1.572 1.630 0.200
ENSG00000251322 E039 376.8277430 1.163868097 3.955506e-01 0.6248414601 22 50720184 50722437 2254 + 2.326 2.579 0.842
ENSG00000251322 E040 64.4057727 0.251929927 2.748964e-01 0.5148978274 22 50723398 50723551 154 + 1.599 1.811 0.718
ENSG00000251322 E041 10.9822388 0.013011192 6.307708e-01 0.7908816850 22 50724127 50724153 27 + 1.020 1.043 0.083
ENSG00000251322 E042 37.9747540 0.166184811 3.642823e-01 0.5992449782 22 50729572 50729707 136 + 1.379 1.588 0.716
ENSG00000251322 E043 22.3521408 0.001676257 2.890420e-03 0.0227471074 22 50730721 50730802 82 + 1.021 1.398 1.336
ENSG00000251322 E044 11.9566286 0.002696937 7.031349e-06 0.0001544142 22 50730803 50730946 144 + 0.412 1.176 3.141
ENSG00000251322 E045 8.8431727 0.003542884 2.132642e-03 0.0179621934 22 50730947 50730982 36 + 0.493 1.043 2.249
ENSG00000251322 E046 82.5046967 0.243457461 1.092010e-01 0.2977800283 22 50730983 50731710 728 + 1.592 1.939 1.174
ENSG00000251322 E047 306.7634742 1.039801847 3.415637e-01 0.5794318414 22 50731711 50733027 1317 + 2.207 2.495 0.962
ENSG00000251322 E048 42.8019005 0.165580061 4.869372e-01 0.6918454953 22 50733028 50733212 185 + 1.498 1.620 0.416