ENSG00000250479

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000484558 ENSG00000250479 HEK293_OSMI2_6hA HEK293_TMG_6hB CHCHD10 protein_coding protein_coding 265.8975 385.8393 155.0239 99.90882 6.899281 -1.315454 109.3612 197.4802 38.02435 57.14704 1.248841 -2.3764063 0.3978833 0.5015 0.2468000 -0.2547000 1.800684e-06 2.532178e-07 FALSE TRUE
ENST00000520222 ENSG00000250479 HEK293_OSMI2_6hA HEK293_TMG_6hB CHCHD10 protein_coding protein_coding 265.8975 385.8393 155.0239 99.90882 6.899281 -1.315454 151.9390 183.4019 114.71644 42.79581 7.395084 -0.6768895 0.5824208 0.4841 0.7387667 0.2546667 2.532178e-07 2.532178e-07 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000250479 E001 566.929731 2.518241e-03 3.674305e-01 6.019394e-01 22 23765834 23765873 40 - 2.654 2.634 -0.066
ENSG00000250479 E002 1298.291083 4.632639e-04 2.617860e-09 1.400229e-07 22 23765874 23765934 61 - 3.064 2.985 -0.261
ENSG00000250479 E003 1790.738115 7.963680e-05 1.216989e-10 8.477140e-09 22 23765935 23766026 92 - 3.186 3.134 -0.173
ENSG00000250479 E004 1680.840451 8.068432e-05 1.062102e-01 2.927027e-01 22 23766128 23766275 148 - 3.121 3.115 -0.022
ENSG00000250479 E005 7.984269 6.132942e-01 1.000000e+00 1.000000e+00 22 23766276 23766296 21 - 0.765 0.866 0.396
ENSG00000250479 E006 32.788219 3.838583e-02 1.489036e-01 3.607312e-01 22 23766297 23766464 168 - 1.561 1.382 -0.616
ENSG00000250479 E007 581.125138 1.741134e-03 9.711582e-11 6.903516e-09 22 23767374 23767540 167 - 2.472 2.680 0.694
ENSG00000250479 E008 369.783099 2.751176e-03 9.404095e-22 3.390725e-19 22 23767541 23767593 53 - 2.086 2.504 1.394
ENSG00000250479 E009 283.549500 4.111780e-03 3.565322e-14 4.809163e-12 22 23767834 23767972 139 - 2.002 2.387 1.289