ENSG00000229809

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000223459 ENSG00000229809 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF688 protein_coding protein_coding 14.03423 23.18787 3.751792 4.304105 0.2762004 -2.6245 10.3440041 17.8632290 2.3419627 3.2519428 0.25554782 -2.9258632 0.7132750 0.77120000 0.6216333 -0.14956667 6.266225e-02 5.304933e-06 FALSE TRUE
ENST00000563276 ENSG00000229809 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF688 protein_coding protein_coding 14.03423 23.18787 3.751792 4.304105 0.2762004 -2.6245 0.4556314 0.6880525 0.5996173 0.2264180 0.01235763 -0.1954318 0.0455750 0.02823333 0.1620000 0.13376667 5.304933e-06 5.304933e-06 FALSE TRUE
ENST00000563665 ENSG00000229809 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF688 protein_coding retained_intron 14.03423 23.18787 3.751792 4.304105 0.2762004 -2.6245 1.6117102 2.2766454 0.6413599 0.2301137 0.09110030 -1.8117057 0.1331625 0.10200000 0.1715667 0.06956667 3.046568e-01 5.304933e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000229809 E001 0.4646582 0.0222263722 2.129624e-01   16 30569672 30569685 14 - 0.290 0.086 -2.116
ENSG00000229809 E002 5.9139345 0.0057022293 3.914861e-03 0.0286677367 16 30569686 30569725 40 - 1.021 0.609 -1.632
ENSG00000229809 E003 150.4585493 0.0858457252 4.333175e-01 0.6536965312 16 30569726 30570177 452 - 1.911 1.969 0.195
ENSG00000229809 E004 69.2058806 0.0007326715 9.354973e-01 0.9699019300 16 30570178 30570219 42 - 1.611 1.644 0.113
ENSG00000229809 E005 153.0301311 0.0003554152 2.200977e-01 0.4536342532 16 30570220 30570436 217 - 1.907 1.987 0.269
ENSG00000229809 E006 2.0598763 0.0261525444 1.000000e+00 1.0000000000 16 30570734 30570898 165 - 0.290 0.344 0.349
ENSG00000229809 E007 2.6663614 0.0091320969 6.952389e-01 0.8324140330 16 30570899 30570940 42 - 0.290 0.423 0.793
ENSG00000229809 E008 107.8676207 0.0015366445 4.395320e-01 0.6583076824 16 30571010 30571123 114 - 1.840 1.826 -0.048
ENSG00000229809 E009 10.8968577 0.0114645058 5.326379e-02 0.1873811872 16 30571124 30571224 101 - 1.093 0.847 -0.915
ENSG00000229809 E010 10.2220679 0.1532543377 2.995248e-01 0.5399521693 16 30571225 30571292 68 - 0.982 0.839 -0.544
ENSG00000229809 E011 15.4634150 0.0026552301 6.137143e-01 0.7793813907 16 30571293 30571433 141 - 1.059 1.022 -0.135
ENSG00000229809 E012 93.2410145 0.0990585117 4.756052e-01 0.6839183326 16 30571434 30571714 281 - 1.574 1.775 0.683
ENSG00000229809 E013 7.2274358 0.0045118785 3.347071e-05 0.0005927107 16 30572122 30572734 613 - 1.183 0.653 -2.024