ENSG00000227345

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000614063 ENSG00000227345 HEK293_OSMI2_6hA HEK293_TMG_6hB PARG protein_coding nonsense_mediated_decay 10.60084 5.422684 17.28529 0.9218875 0.786433 1.670642 1.656213 0.2248225 3.674926 0.1398385 0.4481185 3.971993 0.1068500 0.0551000 0.2114667 0.15636667 0.2703816957 0.0007952825 FALSE TRUE
ENST00000616448 ENSG00000227345 HEK293_OSMI2_6hA HEK293_TMG_6hB PARG protein_coding protein_coding 10.60084 5.422684 17.28529 0.9218875 0.786433 1.670642 5.357783 3.5192209 7.822498 0.6497797 0.3674178 1.150123 0.5454167 0.6447333 0.4525667 -0.19216667 0.0007952825 0.0007952825 FALSE TRUE
MSTRG.3998.4 ENSG00000227345 HEK293_OSMI2_6hA HEK293_TMG_6hB PARG protein_coding   10.60084 5.422684 17.28529 0.9218875 0.786433 1.670642 2.593945 1.3474462 4.999230 0.3228193 0.2420619 1.883694 0.2306458 0.2410000 0.2906667 0.04966667 0.7677445624 0.0007952825 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000227345 E001 26.2869980 0.0014706122 8.881619e-02 2.615902e-01 10 49818279 49818501 223 - 1.360 1.475 0.398
ENSG00000227345 E002 69.0793847 0.0007366125 5.353343e-05 8.828987e-04 10 49818502 49818629 128 - 1.747 1.920 0.583
ENSG00000227345 E003 90.3465284 0.0008263834 3.384192e-06 8.238440e-05 10 49818630 49818889 260 - 1.859 2.037 0.597
ENSG00000227345 E004 64.4120521 0.0096739401 2.960685e-02 1.261681e-01 10 49818890 49818991 102 - 1.728 1.870 0.481
ENSG00000227345 E005 61.5925154 0.0007565403 2.004573e-03 1.709807e-02 10 49818992 49819066 75 - 1.711 1.851 0.473
ENSG00000227345 E006 43.1755686 0.0009232176 2.040651e-01 4.347071e-01 10 49819067 49819144 78 - 1.592 1.658 0.226
ENSG00000227345 E007 52.2202781 0.0010163556 3.620881e-01 5.974107e-01 10 49819145 49819275 131 - 1.681 1.723 0.142
ENSG00000227345 E008 18.9535531 0.0057801792 5.517348e-01 7.374949e-01 10 49819276 49819277 2 - 1.255 1.302 0.164
ENSG00000227345 E009 101.8188963 0.0004175095 7.833399e-01 8.866068e-01 10 49819278 49819494 217 - 1.975 1.980 0.016
ENSG00000227345 E010 47.6139039 0.0008977759 8.540433e-02 2.553485e-01 10 49820165 49820209 45 - 1.624 1.711 0.296
ENSG00000227345 E011 64.6503694 0.0006264088 9.630948e-01 9.835035e-01 10 49820210 49820293 84 - 1.784 1.776 -0.025
ENSG00000227345 E012 76.5195284 0.0008698721 3.583714e-01 5.945016e-01 10 49832803 49832908 106 - 1.842 1.877 0.118
ENSG00000227345 E013 1.8401644 0.0110616422 2.879797e-01 5.287603e-01 10 49832909 49833274 366 - 0.357 0.538 0.943
ENSG00000227345 E014 84.1564242 0.0005961825 6.261227e-01 7.880268e-01 10 49841950 49842058 109 - 1.887 1.902 0.050
ENSG00000227345 E015 74.1874155 0.0006256546 7.206883e-01 8.486315e-01 10 49843554 49843632 79 - 1.848 1.827 -0.073
ENSG00000227345 E016 0.1614157 0.0332502471 1.000000e+00   10 49857295 49857305 11 - 0.084 0.000 -8.705
ENSG00000227345 E017 86.6162077 0.0047178566 4.377851e-01 6.570510e-01 10 49857306 49857453 148 - 1.925 1.877 -0.161
ENSG00000227345 E018 57.3981107 0.0044491443 2.114983e-01 4.435491e-01 10 49861588 49861663 76 - 1.759 1.679 -0.271
ENSG00000227345 E019 38.4862191 0.0022815940 5.963583e-02 2.019449e-01 10 49865321 49865381 61 - 1.603 1.474 -0.441
ENSG00000227345 E020 0.4820342 0.0213247385 3.180047e-01   10 49867072 49867434 363 - 0.214 0.000 -10.277
ENSG00000227345 E021 52.2363916 0.0012969061 2.636980e-01 5.029819e-01 10 49869476 49869555 80 - 1.711 1.644 -0.228
ENSG00000227345 E022 73.4163667 0.0007017581 2.737097e-01 5.136097e-01 10 49879673 49879830 158 - 1.850 1.794 -0.189
ENSG00000227345 E023 45.8293395 0.0008515444 2.327995e-01 4.683819e-01 10 49885203 49885295 93 - 1.617 1.677 0.203
ENSG00000227345 E024 1.1254840 0.0143064023 5.179409e-02   10 49908455 49908541 87 - 0.396 0.000 -11.463
ENSG00000227345 E025 52.0903036 0.0006921051 1.236259e-01 3.214659e-01 10 49915917 49915991 75 - 1.666 1.739 0.249
ENSG00000227345 E026 47.7613750 0.0009408948 9.079082e-02 2.651984e-01 10 49922336 49922419 84 - 1.622 1.707 0.290
ENSG00000227345 E027 58.8069034 0.0042832411 7.074794e-01 8.402347e-01 10 49922547 49922669 123 - 1.736 1.754 0.059
ENSG00000227345 E028 101.4884699 0.0003843660 1.411638e-02 7.480997e-02 10 49932100 49932283 184 - 2.005 1.905 -0.338
ENSG00000227345 E029 256.8328071 0.0020442109 7.369500e-08 2.825081e-06 10 49933177 49934163 987 - 2.425 2.251 -0.579
ENSG00000227345 E030 27.7552403 0.0031093317 1.943882e-03 1.669171e-02 10 49935076 49935142 67 - 1.488 1.234 -0.883
ENSG00000227345 E031 51.7950016 0.0110987796 9.261856e-01 9.652301e-01 10 49941509 49941785 277 - 1.695 1.679 -0.055
ENSG00000227345 E032 25.6658899 0.0051889237 6.900968e-02 2.227902e-01 10 49941786 49941824 39 - 1.437 1.280 -0.546
ENSG00000227345 E033 34.8216122 0.0010920960 3.929069e-01 6.227333e-01 10 49941825 49941934 110 - 1.537 1.475 -0.210
ENSG00000227345 E034 20.4758708 0.0023424561 1.931768e-01 4.210197e-01 10 49941935 49942027 93 - 1.327 1.209 -0.414
ENSG00000227345 E035 0.0000000       10 49970167 49970203 37 -