ENSG00000226328

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000426282 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA lncRNA 4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.6403484 0.59667211 0.21080696 0.13000165 0.09220913 -1.4581313 0.13169583 0.08916667 0.15253333 0.06336667 0.909674483 0.002534981 FALSE TRUE
ENST00000432502 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA lncRNA 4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.2072609 0.53506706 0.00000000 0.53506706 0.00000000 -5.7683618 0.03251250 0.07203333 0.00000000 -0.07203333 1.000000000 0.002534981   FALSE
ENST00000609284 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA lncRNA 4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.1240356 0.08283799 0.19934235 0.08283799 0.19934235 1.1730770 0.03115833 0.01073333 0.07486667 0.06413333 0.844179892 0.002534981   FALSE
MSTRG.22310.11 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA   4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.7878998 1.05093809 0.51504292 0.24254407 0.26032723 -1.0148332 0.15530417 0.14573333 0.24920000 0.10346667 0.932987163 0.002534981   FALSE
MSTRG.22310.2 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA   4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 1.3030181 1.61944656 0.22845134 0.41690903 0.22845134 -2.7726152 0.24530833 0.23606667 0.08580000 -0.15026667 0.516501656 0.002534981   FALSE
MSTRG.22310.4 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA   4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.1423335 0.17954877 0.21134495 0.10132069 0.21134495 0.2237273 0.05592083 0.02816667 0.22320000 0.19503333 0.977444150 0.002534981   FALSE
MSTRG.22310.8 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA   4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.8979197 1.50324062 0.05444529 0.23155136 0.05444529 -4.5534227 0.16930833 0.21126667 0.02046667 -0.19080000 0.002534981 0.002534981   FALSE
MSTRG.22310.9 ENSG00000226328 HEK293_OSMI2_6hA HEK293_TMG_6hB NUP50-DT lncRNA   4.940048 7.023386 1.747483 0.5538752 0.4984738 -2.000709 0.3159103 0.55536303 0.07014289 0.36042652 0.07014289 -2.8185311 0.06683333 0.07590000 0.02633333 -0.04956667 0.803331435 0.002534981   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000226328 E001 7.6585975 0.005881017 0.5038061681 0.703438969 22 45133020 45133700 681 - 0.761 0.844 0.326
ENSG00000226328 E002 0.9574634 0.015612700 0.4138182280   22 45133701 45133705 5 - 0.373 0.207 -1.155
ENSG00000226328 E003 32.2504058 0.065105859 0.1338027939 0.337734719 22 45133706 45134289 584 - 1.554 1.371 -0.633
ENSG00000226328 E004 8.4125842 0.089800181 0.9479615959 0.976244714 22 45134290 45134295 6 - 0.850 0.877 0.106
ENSG00000226328 E005 25.5076821 0.001519415 0.3051387097 0.545757821 22 45134296 45134519 224 - 1.256 1.323 0.237
ENSG00000226328 E006 10.0303360 0.022618714 0.0700101609 0.224829426 22 45134520 45134520 1 - 0.707 0.974 1.041
ENSG00000226328 E007 40.4537138 0.013222346 0.1090002457 0.297404424 22 45134521 45134797 277 - 1.408 1.524 0.398
ENSG00000226328 E008 14.1021784 0.014838282 0.2884384874 0.529157072 22 45138824 45138916 93 - 0.962 1.080 0.433
ENSG00000226328 E009 3.2000704 0.083100157 0.6867781300 0.827033338 22 45138917 45139757 841 - 0.483 0.567 0.395
ENSG00000226328 E010 0.1426347 0.031267812 0.0980688014   22 45141696 45141822 127 - 0.225 0.000 -11.151
ENSG00000226328 E011 2.2604833 0.009030272 0.0441022759 0.165375173 22 45152405 45152449 45 - 0.706 0.347 -1.738
ENSG00000226328 E012 2.9520059 0.008131106 0.3744000425 0.607691950 22 45152450 45152623 174 - 0.644 0.476 -0.774
ENSG00000226328 E013 9.8884413 0.004284858 0.0446316564 0.166691924 22 45158060 45158174 115 - 1.122 0.871 -0.930
ENSG00000226328 E014 5.1433524 0.007911950 0.0003142454 0.003874408 22 45158175 45158185 11 - 1.050 0.538 -2.060
ENSG00000226328 E015 9.7338001 0.032295819 0.2163714792 0.449130800 22 45163518 45163571 54 - 1.078 0.886 -0.711
ENSG00000226328 E016 6.5741130 0.005036823 0.9395029560 0.971960198 22 45163572 45163576 5 - 0.809 0.781 -0.111
ENSG00000226328 E017 18.0613023 0.002139587 0.4478274243 0.664104014 22 45163577 45163662 86 - 1.122 1.178 0.201
ENSG00000226328 E018 12.1597587 0.002931634 0.5526162304 0.738121219 22 45163663 45163666 4 - 0.962 1.015 0.194
ENSG00000226328 E019 24.0933700 0.001935258 0.0765546045 0.237866489 22 45163667 45163810 144 - 1.165 1.307 0.503