ENSG00000225921

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000451315 ENSG00000225921 HEK293_OSMI2_6hA HEK293_TMG_6hB NOL7 protein_coding protein_coding 78.28263 56.10958 92.83602 12.47153 4.683557 0.7263359 51.5951 39.94330 47.98871 9.363698 1.297810 0.2646809 0.6521167 0.7084667 0.5198000 -0.1886667 0.004441543 0.004441543 FALSE TRUE
ENST00000474485 ENSG00000225921 HEK293_OSMI2_6hA HEK293_TMG_6hB NOL7 protein_coding processed_transcript 78.28263 56.10958 92.83602 12.47153 4.683557 0.7263359 25.7553 15.74404 43.37761 3.156765 4.808773 1.4615616 0.3358625 0.2831667 0.4643333 0.1811667 0.004698666 0.004441543 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000225921 E001 558.780791 0.0024920098 8.165195e-11 5.893064e-09 6 13615335 13615546 212 + 2.660 2.835 0.580
ENSG00000225921 E002 482.560007 0.0002419522 5.153387e-09 2.584653e-07 6 13615547 13615588 42 + 2.639 2.743 0.346
ENSG00000225921 E003 489.179730 0.0002292619 1.080602e-06 3.040275e-05 6 13615589 13615624 36 + 2.654 2.741 0.289
ENSG00000225921 E004 525.407945 0.0002816223 3.795942e-01 6.118725e-01 6 13615712 13615772 61 + 2.718 2.739 0.069
ENSG00000225921 E005 497.848017 0.0001983636 5.768914e-01 7.550455e-01 6 13616463 13616521 59 + 2.697 2.712 0.051
ENSG00000225921 E006 441.447316 0.0001685849 1.157715e-01 3.088036e-01 6 13617770 13617801 32 + 2.661 2.640 -0.069
ENSG00000225921 E007 11.131096 0.0093980297 2.168072e-02 1.015440e-01 6 13617802 13618057 256 + 1.186 0.937 -0.907
ENSG00000225921 E008 721.281910 0.0001460351 1.339948e-02 7.202369e-02 6 13618058 13618139 82 + 2.876 2.848 -0.093
ENSG00000225921 E009 3.427358 0.0842045183 4.538152e-02 1.685252e-01 6 13618140 13618217 78 + 0.784 0.381 -1.856
ENSG00000225921 E010 1040.324447 0.0001846009 1.810057e-07 6.310598e-06 6 13620208 13620329 122 + 3.048 2.992 -0.185
ENSG00000225921 E011 687.092863 0.0010988375 6.308829e-03 4.124198e-02 6 13620408 13620485 78 + 2.866 2.810 -0.186
ENSG00000225921 E012 470.756968 0.0005043700 5.050254e-04 5.725678e-03 6 13620754 13620856 103 + 2.706 2.645 -0.206
ENSG00000225921 E013 206.005054 0.0027581083 3.454214e-04 4.192665e-03 6 13620857 13621712 856 + 2.372 2.246 -0.422
ENSG00000225921 E014 11.054148 0.4368672331 9.705462e-01 9.871416e-01 6 13632393 13632739 347 + 1.135 1.012 -0.446