ENSG00000224877

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000431388 ENSG00000224877 HEK293_OSMI2_6hA HEK293_TMG_6hB NDUFAF8 protein_coding protein_coding 278.3759 369.3783 99.62444 86.13776 6.929997 -1.890422 241.976667 352.380198 44.31736 83.799418 5.964304 -2.990904 0.8142042 0.95026667 0.4578333 -0.4924333 1.911770e-06 8.415596e-23   FALSE
ENST00000576002 ENSG00000224877 HEK293_OSMI2_6hA HEK293_TMG_6hB NDUFAF8 protein_coding protein_coding 278.3759 369.3783 99.62444 86.13776 6.929997 -1.890422 26.116957 7.238018 32.10079 4.331176 8.015538 2.147400 0.1292917 0.02133333 0.3147000 0.2933667 2.610795e-01 8.415596e-23   FALSE
ENST00000577158 ENSG00000224877 HEK293_OSMI2_6hA HEK293_TMG_6hB NDUFAF8 protein_coding processed_transcript 278.3759 369.3783 99.62444 86.13776 6.929997 -1.890422 3.324593 0.000000 19.12260 0.000000 5.550352 10.901817 0.0256625 0.00000000 0.1857667 0.1857667 8.415596e-23 8.415596e-23   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000224877 E001 1.689707 0.1072441580 6.264890e-02 2.086941e-01 17 81239191 81239267 77 + 0.685 0.220 -2.547
ENSG00000224877 E002 1.904328 0.0171347674 2.439816e-01 4.809984e-01 17 81239268 81239304 37 + 0.591 0.294 -1.582
ENSG00000224877 E003 3.674566 0.2504107563 9.279030e-01 9.660048e-01 17 81239305 81239310 6 + 0.584 0.491 -0.439
ENSG00000224877 E004 6.210509 0.1761041112 7.110469e-01 8.424913e-01 17 81239311 81239317 7 + 0.825 0.655 -0.691
ENSG00000224877 E005 273.128125 0.0096420725 2.653885e-14 3.673675e-12 17 81239318 81239403 86 + 1.825 2.243 1.403
ENSG00000224877 E006 432.622285 0.0062765203 1.969991e-16 3.620668e-14 17 81239404 81239447 44 + 2.120 2.437 1.056
ENSG00000224877 E007 446.152744 0.0027181560 2.329073e-16 4.221240e-14 17 81239568 81239594 27 + 2.227 2.446 0.730
ENSG00000224877 E008 474.731529 0.0002199828 9.858605e-10 5.761245e-08 17 81239595 81239610 16 + 2.404 2.464 0.200
ENSG00000224877 E009 541.183367 0.0001436897 2.103989e-08 9.185236e-07 17 81239611 81239628 18 + 2.487 2.520 0.110
ENSG00000224877 E010 843.819467 0.0001342338 2.940798e-01 5.346743e-01 17 81239629 81239678 50 + 2.783 2.699 -0.280
ENSG00000224877 E011 13.070611 0.0024613637 7.590370e-01 8.719842e-01 17 81239679 81239682 4 + 1.066 0.936 -0.482
ENSG00000224877 E012 11.804995 0.0238301620 1.619947e-01 3.797163e-01 17 81239683 81239771 89 + 1.164 0.860 -1.122
ENSG00000224877 E013 25.001394 0.0288305693 3.880134e-03 2.847483e-02 17 81239772 81239913 142 + 1.578 1.126 -1.574
ENSG00000224877 E014 14.770756 0.0727308571 2.470454e-02 1.113133e-01 17 81239914 81239943 30 + 1.397 0.911 -1.743
ENSG00000224877 E015 37.683012 0.0554034721 9.620933e-06 2.027368e-04 17 81239944 81240229 286 + 1.922 1.227 -2.378
ENSG00000224877 E016 34.799037 0.0345310876 2.257994e-08 9.793701e-07 17 81240230 81240439 210 + 1.912 1.185 -2.493
ENSG00000224877 E017 2094.740706 0.0099515999 3.947088e-08 1.612864e-06 17 81240987 81241310 324 + 3.329 3.061 -0.892