ENSG00000223959

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000423684 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene retained_intron 18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 3.7693483 2.1975663 3.6016280 1.1219813 0.86999494 0.71019253 0.20820417 0.11813333 0.18023333 0.062100000 8.409356e-01 3.450296e-08 FALSE  
ENST00000427658 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene retained_intron 18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 1.1514531 1.1001443 0.7825201 0.5447103 0.05919975 -0.48622779 0.05910833 0.05630000 0.03943333 -0.016866667 9.194090e-01 3.450296e-08 TRUE  
ENST00000436447 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene processed_transcript 18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 0.4421218 0.8868149 0.0000000 0.1668237 0.00000000 -6.48673827 0.02671667 0.05283333 0.00000000 -0.052833333 3.450296e-08 3.450296e-08 TRUE  
ENST00000450026 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene retained_intron 18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 0.9962588 1.4983627 0.1801361 1.0863186 0.18013606 -2.98787927 0.04967500 0.07123333 0.00890000 -0.062333333 6.316835e-01 3.450296e-08 FALSE  
ENST00000458301 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene processed_transcript 18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 1.2279713 0.8235065 1.7373200 0.4424105 0.87357766 1.06787846 0.06066667 0.04180000 0.08756667 0.045766667 9.552877e-01 3.450296e-08    
ENST00000690928 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene processed_transcript 18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 1.4574456 1.6644864 1.5088605 0.3880614 0.39127953 -0.14072928 0.08425833 0.10206667 0.07550000 -0.026566667 9.015283e-01 3.450296e-08 FALSE  
MSTRG.13292.12 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene   18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 1.5673113 1.5714451 1.6569835 0.2725852 0.16315059 0.07599629 0.08681250 0.09000000 0.08316667 -0.006833333 9.628832e-01 3.450296e-08 TRUE  
MSTRG.13292.17 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene   18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 0.1798807 0.7666026 0.0000000 0.3835772 0.00000000 -6.27910461 0.01168333 0.05160000 0.00000000 -0.051600000 3.420623e-01 3.450296e-08 TRUE  
MSTRG.13292.25 ENSG00000223959 HEK293_OSMI2_6hA HEK293_TMG_6hB AFG3L1P transcribed_unitary_pseudogene   18.46252 17.54901 19.88428 2.660269 0.2397104 0.1801417 1.3062642 0.8537012 1.8843034 0.5650447 0.56288710 1.13306318 0.07608333 0.05483333 0.09520000 0.040366667 8.126770e-01 3.450296e-08 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000223959 E001 0.7614362 0.8281578859 0.9098461628   16 89969087 89969191 105 + 0.243 0.211 -0.261
ENSG00000223959 E002 0.4054685 0.7893016189 0.4664730984   16 89969192 89969275 84 + 0.000 0.211 10.939
ENSG00000223959 E003 0.0000000       16 89969276 89969478 203 +      
ENSG00000223959 E004 0.1779838 0.0352052845 0.3900594834   16 89969479 89969570 92 + 0.134 0.000 -12.256
ENSG00000223959 E005 0.0000000       16 89969571 89969623 53 +      
ENSG00000223959 E006 0.1779838 0.0352052845 0.3900594834   16 89969624 89969755 132 + 0.134 0.000 -12.256
ENSG00000223959 E007 0.0000000       16 89969756 89969779 24 +      
ENSG00000223959 E008 0.0000000       16 89969780 89969824 45 +      
ENSG00000223959 E009 0.9408952 0.0160314948 0.6060739117   16 89969825 89972580 2756 + 0.236 0.333 0.677
ENSG00000223959 E010 0.2027342 0.0489879780 0.6008337818   16 89972581 89972581 1 + 0.000 0.110 11.065
ENSG00000223959 E011 0.5834523 0.5662871668 0.8119887487   16 89972582 89972585 4 + 0.136 0.208 0.744
ENSG00000223959 E012 0.5834523 0.5662871668 0.8119887487   16 89972586 89972588 3 + 0.136 0.208 0.744
ENSG00000223959 E013 0.5834523 0.5662871668 0.8119887487   16 89972589 89972589 1 + 0.136 0.208 0.744
ENSG00000223959 E014 1.5874112 0.2193836393 0.5731442066 0.752414318 16 89972590 89972592 3 + 0.323 0.443 0.684
ENSG00000223959 E015 1.5874112 0.2193836393 0.5731442066 0.752414318 16 89972593 89972593 1 + 0.323 0.443 0.684
ENSG00000223959 E016 1.5874112 0.2193836393 0.5731442066 0.752414318 16 89972594 89972595 2 + 0.323 0.443 0.684
ENSG00000223959 E017 5.3049173 0.0089583416 0.0346804377 0.140737699 16 89972596 89972607 12 + 0.590 0.899 1.263
ENSG00000223959 E018 9.6453904 0.0034320302 0.0001039173 0.001544509 16 89972608 89972615 8 + 0.696 1.164 1.774
ENSG00000223959 E019 9.6453904 0.0034320302 0.0001039173 0.001544509 16 89972616 89972616 1 + 0.696 1.164 1.774
ENSG00000223959 E020 15.0799088 0.0086584475 0.0037381688 0.027680932 16 89972617 89972630 14 + 1.001 1.304 1.087
ENSG00000223959 E021 17.6130871 0.0172899415 0.0061700630 0.040562374 16 89972631 89972647 17 + 1.072 1.369 1.050
ENSG00000223959 E022 42.3156053 0.0208806316 0.1009685484 0.283539733 16 89972648 89972765 118 + 1.543 1.689 0.497
ENSG00000223959 E023 5.4424353 0.1142806876 0.3991260898 0.627516047 16 89977605 89977638 34 + 0.669 0.875 0.823
ENSG00000223959 E024 30.0931595 0.0012045459 0.1036777055 0.288334518 16 89977639 89977677 39 + 1.419 1.537 0.406
ENSG00000223959 E025 31.3532727 0.0011874319 0.7822758051 0.886002834 16 89977678 89977693 16 + 1.485 1.515 0.103
ENSG00000223959 E026 32.2234332 0.0011223536 0.8940682713 0.948729713 16 89977694 89977714 21 + 1.506 1.526 0.072
ENSG00000223959 E027 62.1169118 0.0010651324 0.5100223628 0.707987382 16 89977715 89977802 88 + 1.807 1.790 -0.059
ENSG00000223959 E028 35.2503525 0.0915481238 0.9089950433 0.956575112 16 89977803 89978517 715 + 1.479 1.606 0.433
ENSG00000223959 E029 55.3588167 0.0146266026 0.0232316929 0.106689629 16 89978810 89978878 69 + 1.829 1.672 -0.530
ENSG00000223959 E030 13.4483655 0.0624898881 0.2492229417 0.487017196 16 89979302 89979714 413 + 1.208 1.106 -0.364
ENSG00000223959 E031 29.6514920 0.0519869184 0.2947610991 0.535296848 16 89979715 89980241 527 + 1.516 1.456 -0.207
ENSG00000223959 E032 48.6871546 0.0298418292 0.0385328108 0.150948249 16 89980242 89980244 3 + 1.790 1.594 -0.663
ENSG00000223959 E033 86.4004767 0.0083623991 0.0269779802 0.118244823 16 89980245 89980336 92 + 1.997 1.882 -0.387
ENSG00000223959 E034 91.3908258 0.0140106778 0.1859640384 0.411810023 16 89981773 89981918 146 + 2.004 1.924 -0.269
ENSG00000223959 E035 30.1944220 0.0635030218 0.2956868627 0.536186801 16 89981919 89981923 5 + 1.561 1.420 -0.484
ENSG00000223959 E036 58.8911588 0.0229920212 0.2541231423 0.492546673 16 89984531 89984599 69 + 1.823 1.730 -0.314
ENSG00000223959 E037 26.6212357 0.0013565214 0.0053307412 0.036342560 16 89984600 89984606 7 + 1.529 1.355 -0.601
ENSG00000223959 E038 26.3934907 0.0014462594 0.0104698503 0.060149089 16 89984607 89984617 11 + 1.520 1.360 -0.550
ENSG00000223959 E039 16.8813696 0.0019693089 0.3261402645 0.565320222 16 89984618 89984622 5 + 1.288 1.220 -0.240
ENSG00000223959 E040 20.6333828 0.0019680919 0.3740997986 0.607414007 16 89984623 89984648 26 + 1.362 1.308 -0.187
ENSG00000223959 E041 14.0129166 0.0275228873 0.4908871098 0.694577796 16 89984649 89984656 8 + 1.199 1.154 -0.160
ENSG00000223959 E042 18.2615293 0.0816520211 0.0782736598 0.241351587 16 89984657 89984703 47 + 1.375 1.191 -0.646
ENSG00000223959 E043 25.9918376 0.0050925846 0.1493528691 0.361314457 16 89985446 89985570 125 + 1.470 1.379 -0.314
ENSG00000223959 E044 18.5127931 0.4856602223 0.6376944805 0.795521885 16 89987138 89987176 39 + 1.201 1.345 0.506
ENSG00000223959 E045 5.5587970 0.0419303751 0.8408776708 0.919938223 16 89988496 89988509 14 + 0.782 0.833 0.202
ENSG00000223959 E046 21.2477149 0.0730651041 0.3545631495 0.591364641 16 89988510 89988629 120 + 1.382 1.314 -0.235
ENSG00000223959 E047 17.0430654 0.1172824594 0.1208646635 0.317213237 16 89988630 89988681 52 + 1.340 1.170 -0.599
ENSG00000223959 E048 24.3625751 0.1004658315 0.1509509398 0.363662616 16 89988682 89988851 170 + 1.464 1.344 -0.416
ENSG00000223959 E049 48.4908373 0.0012420120 0.9243837302 0.964516592 16 89988852 89988951 100 + 1.684 1.692 0.028
ENSG00000223959 E050 11.1942430 0.0212991251 0.2729151723 0.512736451 16 89990332 89990865 534 + 0.986 1.146 0.580
ENSG00000223959 E051 53.8064991 0.0040327934 0.1830806802 0.407926085 16 89990866 89991003 138 + 1.684 1.769 0.289
ENSG00000223959 E052 5.3875674 0.0329396426 0.1085323683 0.296618694 16 89991229 89991383 155 + 0.627 0.901 1.103
ENSG00000223959 E053 46.3228433 0.0011163386 0.0622653518 0.207873100 16 89992721 89992828 108 + 1.602 1.714 0.383
ENSG00000223959 E054 30.3238866 0.0014068852 0.7491650851 0.866263097 16 89993768 89993785 18 + 1.470 1.504 0.117
ENSG00000223959 E055 32.4567437 0.0013571031 0.6888172938 0.828332856 16 89993786 89993805 20 + 1.496 1.534 0.132
ENSG00000223959 E056 47.5570927 0.0009999471 0.6224847505 0.785523966 16 89993806 89993893 88 + 1.664 1.702 0.130
ENSG00000223959 E057 12.5788101 0.0082597640 0.1949889870 0.423339488 16 89994693 89994758 66 + 1.045 1.189 0.517
ENSG00000223959 E058 228.3858117 0.0075593763 0.1988644483 0.428249521 16 89994759 89995816 1058 + 2.385 2.338 -0.155
ENSG00000223959 E059 94.5653541 1.1499641787 0.4716185150 0.681284157 16 89995817 89996043 227 + 1.873 2.044 0.576
ENSG00000223959 E060 168.7384408 1.3252552894 0.4079623132 0.634427478 16 89996044 89996626 583 + 2.064 2.321 0.859
ENSG00000223959 E061 0.1426347 0.0312871433 0.3914815292   16 89996783 89996892 110 + 0.134 0.000 -12.271
ENSG00000223959 E062 1.2471745 0.6103487624 0.5120909090   16 89998606 89999195 590 + 0.458 0.255 -1.223
ENSG00000223959 E063 0.1308682 0.0320641978 0.6027616793   16 89999196 89999311 116 + 0.000 0.110 11.136
ENSG00000223959 E064 0.4397201 0.1985078558 0.7730232664   16 89999312 89999823 512 + 0.135 0.194 0.632
ENSG00000223959 E065 1.2978584 0.0136508195 0.0108009958   16 89999824 90000024 201 + 0.547 0.110 -3.130
ENSG00000223959 E066 2.0629987 0.0856682608 0.1049815114 0.290606308 16 90000103 90000297 195 + 0.631 0.330 -1.526
ENSG00000223959 E067 2.7424945 0.0117993814 0.0967432669 0.276028760 16 90000449 90002161 1713 + 0.388 0.676 1.375