ENSG00000223508

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000606507 ENSG00000223508 HEK293_OSMI2_6hA HEK293_TMG_6hB   transcribed_processed_pseudogene processed_transcript 3.072669 2.824395 3.014208 0.3326004 0.2986772 0.09351644 0.8903499 1.69048466 0.2002801 0.51685338 0.11973623 -3.0155621 0.3052167 0.57940000 0.06776667 -0.51163333 0.04223745 0.04223745   FALSE
ENST00000606975 ENSG00000223508 HEK293_OSMI2_6hA HEK293_TMG_6hB   transcribed_processed_pseudogene processed_transcript 3.072669 2.824395 3.014208 0.3326004 0.2986772 0.09351644 0.9352576 0.47361574 1.4215359 0.04873504 0.34358145 1.5656308 0.3024750 0.16880000 0.49926667 0.33046667 0.23012375 0.04223745 FALSE TRUE
MSTRG.31013.1 ENSG00000223508 HEK293_OSMI2_6hA HEK293_TMG_6hB   transcribed_processed_pseudogene   3.072669 2.824395 3.014208 0.3326004 0.2986772 0.09351644 0.6819520 0.53757437 0.8444937 0.32265683 0.47590879 0.6420149 0.2124000 0.20183333 0.26150000 0.05966667 0.98480316 0.04223745   FALSE
MSTRG.31013.3 ENSG00000223508 HEK293_OSMI2_6hA HEK293_TMG_6hB   transcribed_processed_pseudogene   3.072669 2.824395 3.014208 0.3326004 0.2986772 0.09351644 0.3513234 0.08500082 0.3852584 0.08500082 0.06249911 2.0567844 0.1042375 0.03863333 0.12643333 0.08780000 0.39105884 0.04223745 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000223508 E001 1.358130 0.055016564 4.926530e-01   8 86422 86580 159 - 0.288 0.426 0.825
ENSG00000223508 E002 5.101972 0.007157059 1.581328e-01 3.739725e-01 8 213186 213250 65 - 0.863 0.696 -0.664
ENSG00000223508 E003 10.179400 0.045273766 4.461721e-02 1.666510e-01 8 213251 213525 275 - 1.167 0.913 -0.931
ENSG00000223508 E004 9.520140 0.005331787 3.261092e-03 2.496908e-02 8 213526 213710 185 - 1.152 0.881 -0.997
ENSG00000223508 E005 0.000000       8 216374 216407 34 -      
ENSG00000223508 E006 4.900712 0.075615464 3.194546e-01 5.592225e-01 8 219290 219417 128 - 0.821 0.702 -0.482
ENSG00000223508 E007 1.773475 0.285824047 3.333269e-01 5.719319e-01 8 223785 223906 122 - 0.507 0.366 -0.740
ENSG00000223508 E008 4.612263 0.009605125 4.041071e-06 9.579381e-05 8 230008 230170 163 - 0.289 0.995 3.231
ENSG00000223508 E009 6.261032 0.212289448 1.512738e-01 3.641925e-01 8 230804 230907 104 - 0.586 1.040 1.803
ENSG00000223508 E010 12.520428 0.006894720 1.197015e-01 3.153693e-01 8 232129 232297 169 - 1.055 1.209 0.553