ENSG00000213588

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395064 ENSG00000213588 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB9 protein_coding protein_coding 17.11534 19.73346 10.87744 2.406385 0.5088488 -0.8587099 13.7008799 16.9647038 9.5523317 1.78977692 0.4833396 -0.827952 0.8257792 0.864700000 0.87800000 0.01330000 1.00000000 0.04449492 FALSE TRUE
ENST00000612407 ENSG00000213588 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB9 protein_coding protein_coding 17.11534 19.73346 10.87744 2.406385 0.5088488 -0.8587099 2.4291350 2.0548819 0.0000000 1.04329112 0.0000000 -7.689915 0.1059875 0.095266667 0.00000000 -0.09526667 0.36837426 0.04449492 FALSE TRUE
MSTRG.28148.3 ENSG00000213588 HEK293_OSMI2_6hA HEK293_TMG_6hB ZBTB9 protein_coding   17.11534 19.73346 10.87744 2.406385 0.5088488 -0.8587099 0.4334879 0.1695212 0.8055756 0.09156723 0.1180429 2.183664 0.0317750 0.009566667 0.07473333 0.06516667 0.04449492 0.04449492 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000213588 E001 3.928508 0.1064977033 0.785445386 0.887812897 6 33453970 33454231 262 + 0.715 0.655 -0.248
ENSG00000213588 E002 1.248199 0.5215273444 0.931215207   6 33454543 33454570 28 + 0.295 0.335 0.258
ENSG00000213588 E003 1.861102 0.0835757197 0.403814282 0.631370634 6 33454571 33454575 5 + 0.527 0.369 -0.824
ENSG00000213588 E004 34.538189 0.0011666762 0.113356603 0.304751356 6 33454576 33454631 56 + 1.444 1.548 0.354
ENSG00000213588 E005 70.538455 0.0007953713 0.278627436 0.518711120 6 33454632 33454775 144 + 1.787 1.837 0.167
ENSG00000213588 E006 163.238239 0.0003170879 0.000559635 0.006218195 6 33455030 33455405 376 + 2.241 2.146 -0.318
ENSG00000213588 E007 75.015703 0.0005241268 0.060427139 0.203791442 6 33455406 33455487 82 + 1.896 1.817 -0.265
ENSG00000213588 E008 91.904366 0.0017288634 0.458440702 0.672074442 6 33455488 33455599 112 + 1.949 1.920 -0.100
ENSG00000213588 E009 794.681138 0.0002174054 0.004304718 0.030861199 6 33455600 33457544 1945 + 2.846 2.872 0.086