ENSG00000204138

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373839 ENSG00000204138 HEK293_OSMI2_6hA HEK293_TMG_6hB PHACTR4 protein_coding protein_coding 22.21966 26.08856 18.74085 2.508356 0.5962249 -0.4770143 10.6292170 14.044116 8.781990 1.1862711 0.4926051 -0.6767310 0.47955833 0.53966667 0.4681667 -0.0715000 4.083845e-01 4.443047e-27 FALSE TRUE
ENST00000632964 ENSG00000204138 HEK293_OSMI2_6hA HEK293_TMG_6hB PHACTR4 protein_coding processed_transcript 22.21966 26.08856 18.74085 2.508356 0.5962249 -0.4770143 4.1437410 5.898483 0.000000 0.9689482 0.0000000 -9.2066440 0.16512500 0.22316667 0.0000000 -0.2231667 4.443047e-27 4.443047e-27   FALSE
MSTRG.684.4 ENSG00000204138 HEK293_OSMI2_6hA HEK293_TMG_6hB PHACTR4 protein_coding   22.21966 26.08856 18.74085 2.508356 0.5962249 -0.4770143 2.4708018 2.206407 3.634037 0.2569103 0.3857953 0.7173147 0.12186250 0.08763333 0.1943333 0.1067000 8.748550e-02 4.443047e-27 TRUE TRUE
MSTRG.684.7 ENSG00000204138 HEK293_OSMI2_6hA HEK293_TMG_6hB PHACTR4 protein_coding   22.21966 26.08856 18.74085 2.508356 0.5962249 -0.4770143 0.6763911 0.000000 2.068483 0.0000000 1.2184210 7.6993872 0.03397917 0.00000000 0.1068000 0.1068000 2.429779e-01 4.443047e-27 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000204138 E001 0.2027342 0.0355461678 6.831903e-01   1 28369470 28369581 112 + 0.000 0.103 8.333
ENSG00000204138 E002 0.3336024 0.0244411696 3.262390e-01   1 28369582 28369608 27 + 0.000 0.187 11.418
ENSG00000204138 E003 0.3336024 0.0244411696 3.262390e-01   1 28369609 28369616 8 + 0.000 0.187 11.418
ENSG00000204138 E004 0.8513456 0.0172671820 3.353877e-01   1 28369617 28369622 6 + 0.142 0.317 1.470
ENSG00000204138 E005 2.3129482 0.0642334391 7.279788e-01 8.530288e-01 1 28369623 28369663 41 + 0.468 0.536 0.329
ENSG00000204138 E006 2.9801530 0.0087321216 2.717200e-01 5.115721e-01 1 28369664 28369686 23 + 0.468 0.652 0.848
ENSG00000204138 E007 5.7768007 0.0053275990 9.584785e-01 9.811197e-01 1 28369687 28369709 23 + 0.809 0.822 0.053
ENSG00000204138 E008 16.4183756 0.0023020027 5.819148e-02 1.987021e-01 1 28369710 28369738 29 + 1.318 1.167 -0.534
ENSG00000204138 E009 16.7468866 0.0035825225 1.889603e-02 9.207186e-02 1 28369739 28369739 1 + 1.349 1.158 -0.671
ENSG00000204138 E010 17.4260455 0.0030263389 1.705330e-02 8.565640e-02 1 28369740 28369743 4 + 1.364 1.174 -0.666
ENSG00000204138 E011 57.9730432 0.0012647497 2.762182e-02 1.201820e-01 1 28369744 28369825 82 + 1.823 1.726 -0.329
ENSG00000204138 E012 10.4773438 0.0061610687 9.802011e-01 9.919176e-01 1 28392842 28392941 100 + 1.045 1.049 0.015
ENSG00000204138 E013 69.6325897 0.0014593972 2.951988e-03 2.311488e-02 1 28407410 28407463 54 + 1.912 1.786 -0.426
ENSG00000204138 E014 3.4193207 0.1955287694 9.732029e-01 9.885650e-01 1 28432931 28433046 116 + 0.615 0.638 0.099
ENSG00000204138 E015 0.3088520 0.0269401021 7.739209e-01   1 28438151 28438170 20 + 0.143 0.103 -0.537
ENSG00000204138 E016 1.1197889 0.3591191595 3.873332e-01   1 28438171 28438272 102 + 0.144 0.383 1.850
ENSG00000204138 E017 5.2164925 0.0183589893 8.804524e-01 9.414795e-01 1 28438273 28438430 158 + 0.782 0.768 -0.056
ENSG00000204138 E018 25.8459411 0.0019641207 2.305230e-01 4.657340e-01 1 28457832 28457855 24 + 1.467 1.391 -0.262
ENSG00000204138 E019 6.7218395 0.0384505725 4.650854e-01 6.766035e-01 1 28457856 28457859 4 + 0.940 0.840 -0.382
ENSG00000204138 E020 143.6634329 0.0020394648 4.302275e-07 1.356275e-05 1 28459085 28459258 174 + 2.249 2.076 -0.578
ENSG00000204138 E021 114.3881781 0.0004032746 5.852425e-03 3.901379e-02 1 28460212 28460292 81 + 2.104 2.020 -0.282
ENSG00000204138 E022 152.9493982 0.0052259115 1.643196e-02 8.339882e-02 1 28465685 28465849 165 + 2.238 2.138 -0.332
ENSG00000204138 E023 219.6888800 0.0057176942 2.229031e-01 4.568625e-01 1 28466382 28466768 387 + 2.363 2.318 -0.153
ENSG00000204138 E024 1.6176690 0.0122995852 6.358467e-02 2.107900e-01 1 28466769 28467241 473 + 0.570 0.256 -1.757
ENSG00000204138 E025 330.0110125 0.0002332416 4.641876e-05 7.807243e-04 1 28473554 28474151 598 + 2.553 2.481 -0.242
ENSG00000204138 E026 229.4105411 0.0002321975 8.215529e-04 8.455671e-03 1 28476107 28476291 185 + 2.396 2.326 -0.235
ENSG00000204138 E027 241.5302747 0.0009210543 1.106523e-03 1.071800e-02 1 28480451 28480604 154 + 2.420 2.344 -0.256
ENSG00000204138 E028 156.5508858 0.0048744750 1.237247e-01 3.216031e-01 1 28489170 28489225 56 + 2.222 2.162 -0.200
ENSG00000204138 E029 7.8673821 0.0366783474 1.428927e-01 3.518052e-01 1 28489774 28489894 121 + 1.046 0.841 -0.769
ENSG00000204138 E030 150.6077714 0.0038183129 3.266820e-01 5.658549e-01 1 28490951 28491012 62 + 2.189 2.157 -0.107
ENSG00000204138 E031 119.0525249 0.0047730574 1.418116e-01 3.501835e-01 1 28491650 28491694 45 + 2.105 2.047 -0.193
ENSG00000204138 E032 136.5169901 0.0009973218 3.198364e-01 5.595390e-01 1 28491695 28491787 93 + 2.144 2.120 -0.081
ENSG00000204138 E033 111.9384986 0.0004112873 2.267530e-01 4.614597e-01 1 28493015 28493091 77 + 2.013 2.064 0.169
ENSG00000204138 E034 0.8855973 0.0172671820 7.315439e-01   1 28494230 28494322 93 + 0.249 0.316 0.462
ENSG00000204138 E035 0.4820342 0.0213209717 3.978711e-02   1 28494874 28494939 66 + 0.335 0.000 -13.546
ENSG00000204138 E036 454.0823289 0.0005084107 2.353656e-01 4.712808e-01 1 28496534 28497271 738 + 2.659 2.644 -0.048
ENSG00000204138 E037 69.9231345 0.0028143201 1.402025e-01 3.478140e-01 1 28497272 28497458 187 + 1.794 1.876 0.276
ENSG00000204138 E038 173.4941509 0.0003978151 1.373931e-05 2.760591e-04 1 28497459 28497925 467 + 2.151 2.283 0.443
ENSG00000204138 E039 572.5770544 0.0005803731 3.022505e-19 7.912962e-17 1 28497926 28499419 1494 + 2.641 2.809 0.560
ENSG00000204138 E040 538.8254382 0.0022005689 5.146899e-11 3.864111e-09 1 28499420 28500364 945 + 2.610 2.786 0.588