ENSG00000197894

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000296412 ENSG00000197894 HEK293_OSMI2_6hA HEK293_TMG_6hB ADH5 protein_coding protein_coding 78.19651 21.32767 120.8642 1.647754 5.236024 2.502032 22.196853 3.484012 33.17413 0.4895745 2.572904 3.247537 0.2611042 0.1623333 0.2736000 0.11126667 0.02621401 1.072069e-05 FALSE TRUE
ENST00000502590 ENSG00000197894 HEK293_OSMI2_6hA HEK293_TMG_6hB ADH5 protein_coding nonsense_mediated_decay 78.19651 21.32767 120.8642 1.647754 5.236024 2.502032 9.079558 2.877093 13.40603 1.4439797 1.226967 2.216269 0.1125500 0.1354667 0.1118667 -0.02360000 0.97527992 1.072069e-05 FALSE FALSE
ENST00000512621 ENSG00000197894 HEK293_OSMI2_6hA HEK293_TMG_6hB ADH5 protein_coding retained_intron 78.19651 21.32767 120.8642 1.647754 5.236024 2.502032 18.314737 5.268361 32.32495 0.8355110 1.822050 2.614932 0.2289417 0.2455000 0.2671667 0.02166667 0.90733744 1.072069e-05 FALSE TRUE
ENST00000626055 ENSG00000197894 HEK293_OSMI2_6hA HEK293_TMG_6hB ADH5 protein_coding protein_coding 78.19651 21.32767 120.8642 1.647754 5.236024 2.502032 25.709877 7.826848 37.48807 0.5437240 1.495534 2.258471 0.3502375 0.3674667 0.3104000 -0.05706667 0.28944281 1.072069e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000197894 E001 317.3370945 0.0011236343 2.515384e-09 1.353946e-07 4 99070978 99072151 1174 - 2.403 2.204 -0.664
ENSG00000197894 E002 35.0947429 0.0066946664 1.084643e-01 2.964710e-01 4 99072152 99072185 34 - 1.453 1.303 -0.521
ENSG00000197894 E003 442.9993875 0.0001969372 4.714252e-01 6.811692e-01 4 99072186 99072441 256 - 2.521 2.504 -0.058
ENSG00000197894 E004 8.9029435 0.0049620059 1.627426e-01 3.808202e-01 4 99072442 99072572 131 - 0.843 1.023 0.678
ENSG00000197894 E005 537.8753508 0.0001444251 3.156714e-01 5.556815e-01 4 99072573 99072711 139 - 2.601 2.620 0.063
ENSG00000197894 E006 1.8050028 0.1126808733 1.362711e-01 3.417857e-01 4 99074913 99074913 1 - 0.297 0.601 1.605
ENSG00000197894 E007 515.3409165 0.0001915251 1.563329e-02 8.047812e-02 4 99074914 99075049 136 - 2.578 2.626 0.160
ENSG00000197894 E008 24.0103531 0.0470152019 1.898106e-01 4.167372e-01 4 99075225 99076291 1067 - 1.239 1.425 0.647
ENSG00000197894 E009 483.2576537 0.0001530215 3.424039e-01 5.801404e-01 4 99076292 99076391 100 - 2.555 2.574 0.064
ENSG00000197894 E010 359.8253089 0.0001807455 3.174627e-01 5.574151e-01 4 99076392 99076434 43 - 2.426 2.450 0.079
ENSG00000197894 E011 522.8640884 0.0001160027 5.527448e-02 1.919637e-01 4 99076435 99076552 118 - 2.585 2.622 0.124
ENSG00000197894 E012 413.4796625 0.0015037947 2.303979e-01 4.655521e-01 4 99076704 99076796 93 - 2.483 2.516 0.110
ENSG00000197894 E013 344.6363786 0.0002972947 9.480058e-01 9.762547e-01 4 99076797 99076852 56 - 2.411 2.412 0.002
ENSG00000197894 E014 414.4092629 0.0002015453 2.848701e-01 5.254369e-01 4 99076853 99076923 71 - 2.494 2.468 -0.087
ENSG00000197894 E015 1.2567121 0.0135782434 1.508425e-01   4 99079820 99079991 172 - 0.233 0.505 1.632
ENSG00000197894 E016 0.4466850 0.0227943689 8.797455e-01   4 99079992 99080023 32 - 0.154 0.000 -9.838
ENSG00000197894 E017 3.9700552 0.0066687811 5.588820e-01 7.424494e-01 4 99080449 99080725 277 - 0.568 0.669 0.441
ENSG00000197894 E018 369.9292099 0.0007583568 7.328544e-02 2.313816e-01 4 99081365 99081420 56 - 2.448 2.398 -0.167
ENSG00000197894 E019 315.5870879 0.0002092205 3.061733e-01 5.468294e-01 4 99081421 99081452 32 - 2.378 2.350 -0.095
ENSG00000197894 E020 0.1426347 0.0315874057 1.000000e+00   4 99081674 99081918 245 - 0.058 0.000 -8.254
ENSG00000197894 E021 1.7148037 0.0113389826 9.937400e-02 2.806536e-01 4 99081919 99081974 56 - 0.408 0.000 -11.713
ENSG00000197894 E022 321.6429570 0.0004790483 2.397686e-01 4.763806e-01 4 99081975 99082001 27 - 2.387 2.354 -0.112
ENSG00000197894 E023 466.4457444 0.0005914998 9.503310e-01 9.771935e-01 4 99082002 99082116 115 - 2.542 2.540 -0.006
ENSG00000197894 E024 313.7642454 0.0002652273 5.612552e-01 7.441700e-01 4 99085115 99085216 102 - 2.367 2.381 0.048
ENSG00000197894 E025 0.7437705 0.0172671820 2.097921e-01   4 99085414 99085633 220 - 0.154 0.391 1.778
ENSG00000197894 E026 139.2935009 0.0003206863 1.840147e-01 4.091696e-01 4 99088689 99088801 113 - 2.011 2.062 0.170