ENSG00000196865

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369301 ENSG00000196865 HEK293_OSMI2_6hA HEK293_TMG_6hB NHLRC2 protein_coding protein_coding 5.902954 1.843623 12.92841 0.2807014 0.117342 2.803241 3.488882 1.794845 6.736448 0.2554019 0.2168417 1.902254 0.7319542 0.9775333 0.5209000 -0.4566333 1.205902e-09 4.83779e-18 FALSE TRUE
MSTRG.4667.1 ENSG00000196865 HEK293_OSMI2_6hA HEK293_TMG_6hB NHLRC2 protein_coding   5.902954 1.843623 12.92841 0.2807014 0.117342 2.803241 2.271902 0.000000 6.089544 0.0000000 0.1967753 9.252558 0.2208167 0.0000000 0.4710667 0.4710667 4.837790e-18 4.83779e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000196865 E001 18.6376202 0.084806687 0.3482161435 0.585681703 10 113854661 113854823 163 + 1.136 1.252 0.413
ENSG00000196865 E002 38.8994463 0.060328730 0.1441523156 0.353599184 10 113854824 113855050 227 + 1.445 1.525 0.274
ENSG00000196865 E003 49.9670755 0.047488027 0.0080945878 0.049765059 10 113858528 113858680 153 + 1.570 1.513 -0.197
ENSG00000196865 E004 0.8217936 0.016997914 0.4104142186   10 113858681 113858852 172 + 0.146 0.409 1.970
ENSG00000196865 E005 109.4324417 0.104560389 0.0091136904 0.054326731 10 113876521 113876976 456 + 1.912 1.794 -0.398
ENSG00000196865 E006 53.7062773 0.052529486 0.0039910210 0.029106436 10 113879574 113879695 122 + 1.605 1.519 -0.294
ENSG00000196865 E007 63.9304337 0.079456427 0.0239121176 0.108822691 10 113884251 113884380 130 + 1.674 1.620 -0.183
ENSG00000196865 E008 58.1699877 0.073101265 0.0121170750 0.066921136 10 113898110 113898209 100 + 1.638 1.555 -0.284
ENSG00000196865 E009 87.9258094 0.076042766 0.0069093818 0.044223116 10 113901666 113901897 232 + 1.817 1.712 -0.356
ENSG00000196865 E010 53.8582583 0.084044715 0.0315702257 0.131974952 10 113902471 113902593 123 + 1.603 1.550 -0.181
ENSG00000196865 E011 72.8417173 0.067859425 0.0092866575 0.055035177 10 113903527 113903736 210 + 1.734 1.659 -0.255
ENSG00000196865 E012 60.7103560 0.001186897 0.0001965421 0.002636268 10 113904817 113905036 220 + 1.646 1.680 0.116
ENSG00000196865 E013 48.3662260 0.784573813 0.6551717826 0.806808529 10 113908280 113908523 244 + 1.521 1.687 0.567
ENSG00000196865 E014 641.4253583 1.952533159 0.3164205298 0.556443725 10 113908524 113917095 8572 + 2.542 3.036 1.646
ENSG00000196865 E015 10.1486001 0.142733963 0.0386149280 0.151151387 10 113917096 113917194 99 + 0.769 1.352 2.140
ENSG00000196865 E016 0.0000000       10 113921049 113921118 70 +      
ENSG00000196865 E017 0.0000000       10 113926268 113926338 71 +