ENSG00000196646

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343979 ENSG00000196646 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF136 protein_coding protein_coding 1.88585 0.4942044 4.264356 0.02578491 0.008560896 3.083626 1.3529598 0.27500811 3.34220554 0.03478659 0.04685909 3.5560358 0.65085000 0.5592000 0.78376667 0.22456667 0.168323010 0.005057345 FALSE TRUE
ENST00000425827 ENSG00000196646 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF136 protein_coding protein_coding 1.88585 0.4942044 4.264356 0.02578491 0.008560896 3.083626 0.2590390 0.14862996 0.26560351 0.03226478 0.01513057 0.7969290 0.13946250 0.2978000 0.06233333 -0.23546667 0.005057345 0.005057345 FALSE TRUE
ENST00000439995 ENSG00000196646 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF136 protein_coding protein_coding 1.88585 0.4942044 4.264356 0.02578491 0.008560896 3.083626 0.2126287 0.00000000 0.55397673 0.00000000 0.10791593 5.8175637 0.13872500 0.0000000 0.12986667 0.12986667 0.076410943 0.005057345 FALSE TRUE
ENST00000652580 ENSG00000196646 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF136 protein_coding protein_coding 1.88585 0.4942044 4.264356 0.02578491 0.008560896 3.083626 0.0478250 0.05703563 0.06877656 0.01802609 0.06877656 0.2328383 0.05469583 0.1125333 0.01616667 -0.09636667 0.339538169 0.005057345 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000196646 E001 1.9981995 0.0119902721 0.0125194897 0.068507636 19 12163064 12163069 6 + 0.268 0.722 2.319
ENSG00000196646 E002 4.3915419 0.0059308393 0.0193007572 0.093509999 19 12163070 12163095 26 + 0.521 0.894 1.559
ENSG00000196646 E003 8.1680566 0.0044351976 0.2676690699 0.507342483 19 12163096 12163115 20 + 0.779 0.939 0.618
ENSG00000196646 E004 14.0727360 0.0023906755 0.9725114658 0.988234320 19 12163116 12163206 91 + 1.012 1.016 0.017
ENSG00000196646 E005 0.0000000       19 12163760 12163816 57 +      
ENSG00000196646 E006 0.1308682 0.0326491905 0.0481014052   19 12164466 12164628 163 + 0.000 0.267 11.455
ENSG00000196646 E007 0.5117739 0.0217681645 0.3153747458   19 12164629 12164874 246 + 0.095 0.268 1.805
ENSG00000196646 E008 0.6600180 0.0196221008 0.8880402482   19 12169282 12169391 110 + 0.173 0.000 -9.868
ENSG00000196646 E009 0.6653672 0.0234456916 0.0690797174   19 12184618 12185784 1167 + 0.095 0.433 2.807
ENSG00000196646 E010 10.7117386 0.0030465710 0.2029534934 0.433281418 19 12185785 12185828 44 + 0.887 1.051 0.609
ENSG00000196646 E011 15.4075120 0.0023734572 0.3076648533 0.548339911 19 12185829 12185911 83 + 1.069 0.938 -0.484
ENSG00000196646 E012 0.1779838 0.0332876249 0.7865959726   19 12185912 12186113 202 + 0.050 0.000 -7.966
ENSG00000196646 E013 16.8537025 0.0020641028 0.0002949573 0.003678788 19 12186114 12186174 61 + 1.133 0.551 -2.297
ENSG00000196646 E014 177.3149833 0.0005079354 0.5627825465 0.745258343 19 12186570 12189871 3302 + 2.077 2.065 -0.042