ENSG00000196455

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356763 ENSG00000196455 HEK293_OSMI2_6hA HEK293_TMG_6hB PIK3R4 protein_coding protein_coding 18.13525 9.675914 28.16402 3.230174 0.5467513 1.540406 13.095411 5.657165 24.595189 1.5536906 0.9123124 2.1182634 0.6546083 0.6290667 0.8729667 0.24390000 0.02730638 0.02730638 FALSE TRUE
ENST00000511760 ENSG00000196455 HEK293_OSMI2_6hA HEK293_TMG_6hB PIK3R4 protein_coding retained_intron 18.13525 9.675914 28.16402 3.230174 0.5467513 1.540406 1.785106 2.151625 0.000000 1.1940928 0.0000000 -7.7559725 0.1367583 0.1648333 0.0000000 -0.16483333 0.09241445 0.02730638 FALSE TRUE
ENST00000512362 ENSG00000196455 HEK293_OSMI2_6hA HEK293_TMG_6hB PIK3R4 protein_coding retained_intron 18.13525 9.675914 28.16402 3.230174 0.5467513 1.540406 2.692858 1.867123 3.344251 0.5320014 0.6597040 0.8374672 0.1683083 0.2061333 0.1190667 -0.08706667 0.38825913 0.02730638 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000196455 E001 16.5205923 0.0021093544 3.377410e-01 5.760911e-01 3 130678934 130679009 76 - 1.157 1.249 0.325
ENSG00000196455 E002 190.5763526 0.0024292543 5.215011e-04 5.868793e-03 3 130679010 130679485 476 - 2.183 2.315 0.439
ENSG00000196455 E003 4.4316952 0.1976444174 7.860484e-01 8.881200e-01 3 130680521 130680603 83 - 0.658 0.707 0.204
ENSG00000196455 E004 3.3390417 0.0511128743 6.017392e-01 7.712913e-01 3 130680604 130680612 9 - 0.550 0.650 0.443
ENSG00000196455 E005 124.5717532 0.0005818344 4.614898e-05 7.768202e-04 3 130680613 130680721 109 - 1.995 2.142 0.491
ENSG00000196455 E006 109.1728030 0.0005178963 3.386394e-02 1.384952e-01 3 130680977 130681065 89 - 1.956 2.039 0.279
ENSG00000196455 E007 0.1308682 0.0326491905 1.378723e-01   3 130681066 130681071 6 - 0.000 0.197 11.920
ENSG00000196455 E008 129.0037676 0.0003164752 6.334787e-02 2.102469e-01 3 130681491 130681591 101 - 2.033 2.099 0.222
ENSG00000196455 E009 107.7457757 0.0005382525 5.952780e-02 2.017360e-01 3 130684250 130684305 56 - 1.951 2.025 0.250
ENSG00000196455 E010 115.4783241 0.0003569020 1.094968e-01 2.982417e-01 3 130684306 130684381 76 - 1.985 2.046 0.204
ENSG00000196455 E011 206.2989468 0.0002393685 3.623690e-01 5.976362e-01 3 130686211 130686422 212 - 2.245 2.271 0.089
ENSG00000196455 E012 175.5125027 0.0007432855 1.444476e-02 7.604808e-02 3 130690490 130690654 165 - 2.161 2.240 0.263
ENSG00000196455 E013 0.3040503 0.0274424043 9.115857e-01   3 130690655 130690657 3 - 0.134 0.000 -9.602
ENSG00000196455 E014 153.6245161 0.0023939579 2.873514e-02 1.235759e-01 3 130703723 130703888 166 - 2.101 2.186 0.281
ENSG00000196455 E015 164.1736219 0.0003883164 2.129601e-01 4.452246e-01 3 130705561 130705771 211 - 2.142 2.182 0.136
ENSG00000196455 E016 171.7974029 0.0003156317 3.832638e-01 6.149508e-01 3 130706948 130707135 188 - 2.165 2.194 0.094
ENSG00000196455 E017 94.2574045 0.0004292819 9.306320e-01 9.673203e-01 3 130708291 130708321 31 - 1.915 1.913 -0.007
ENSG00000196455 E018 167.4440173 0.0003045167 8.535747e-01 9.268780e-01 3 130708322 130708492 171 - 2.160 2.167 0.025
ENSG00000196455 E019 71.0035061 0.0006135653 5.358534e-01 7.262183e-01 3 130716396 130716416 21 - 1.786 1.817 0.105
ENSG00000196455 E020 66.1585065 0.0006507088 6.815156e-01 8.236085e-01 3 130716417 130716423 7 - 1.759 1.781 0.074
ENSG00000196455 E021 150.4859105 0.0007105378 5.642330e-01 7.462861e-01 3 130716424 130716599 176 - 2.111 2.133 0.073
ENSG00000196455 E022 120.9423914 0.0047378872 1.535775e-01 3.674259e-01 3 130718389 130718530 142 - 2.036 1.970 -0.223
ENSG00000196455 E023 48.3664873 0.0012324565 5.024997e-02 1.802825e-01 3 130718531 130718534 4 - 1.657 1.537 -0.409
ENSG00000196455 E024 122.1163659 0.0004035529 4.553896e-02 1.688877e-01 3 130723414 130723587 174 - 2.042 1.967 -0.251
ENSG00000196455 E025 0.1614157 0.0378537200 1.000000e+00   3 130723694 130723810 117 - 0.072 0.000 -8.603
ENSG00000196455 E026 0.0000000       3 130723811 130723893 83 -      
ENSG00000196455 E027 0.0000000       3 130725899 130725958 60 -      
ENSG00000196455 E028 136.8941345 0.0003429902 2.312246e-01 4.665564e-01 3 130728463 130728684 222 - 2.083 2.042 -0.140
ENSG00000196455 E029 104.3695393 0.0006249703 2.740254e-01 5.139863e-01 3 130730308 130730442 135 - 1.967 1.922 -0.152
ENSG00000196455 E030 250.4721461 0.0012786849 1.519750e-03 1.374786e-02 3 130733548 130734130 583 - 2.358 2.260 -0.329
ENSG00000196455 E031 97.8246932 0.0004696738 2.447473e-07 8.236964e-06 3 130735869 130736002 134 - 1.978 1.742 -0.796
ENSG00000196455 E032 289.7063215 0.0001803489 2.198856e-10 1.449598e-08 3 130744486 130745264 779 - 2.432 2.272 -0.533
ENSG00000196455 E033 134.9031507 0.0019456039 2.328851e-03 1.923788e-02 3 130746318 130746829 512 - 2.099 1.968 -0.438