ENSG00000196177

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358776 ENSG00000196177 HEK293_OSMI2_6hA HEK293_TMG_6hB ACADSB protein_coding protein_coding 8.771126 2.326712 16.15451 0.334303 0.645534 2.790277 5.7330408 1.9355305 8.9996472 0.49712663 0.07158419 2.2113071 0.73054583 0.8011333 0.55900000 -0.24213333 3.909480e-01 1.788825e-13 FALSE TRUE
ENST00000411816 ENSG00000196177 HEK293_OSMI2_6hA HEK293_TMG_6hB ACADSB protein_coding processed_transcript 8.771126 2.326712 16.15451 0.334303 0.645534 2.790277 2.1532080 0.0000000 5.6970584 0.00000000 0.61970110 9.1566035 0.13915000 0.0000000 0.35093333 0.35093333 1.788825e-13 1.788825e-13 FALSE FALSE
ENST00000541070 ENSG00000196177 HEK293_OSMI2_6hA HEK293_TMG_6hB ACADSB protein_coding retained_intron 8.771126 2.326712 16.15451 0.334303 0.645534 2.790277 0.2689757 0.1928185 0.3312226 0.07276464 0.01733308 0.7505237 0.06062917 0.0957000 0.02053333 -0.07516667 2.308585e-01 1.788825e-13   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000196177 E001 0.1614157 0.0336103186 1.000000e+00   10 123008974 123008978 5 + 0.063 0.000 -7.846
ENSG00000196177 E002 0.8346052 0.1710911073 1.000000e+00   10 123008979 123008988 10 + 0.212 0.223 0.094
ENSG00000196177 E003 2.1370981 0.1194390914 2.798307e-01 0.5199252459 10 123008989 123009005 17 + 0.476 0.223 -1.571
ENSG00000196177 E004 16.3649412 0.0023678036 9.528685e-01 0.9784267231 10 123009006 123009067 62 + 1.144 1.176 0.115
ENSG00000196177 E005 16.1979160 0.0023884620 7.270858e-01 0.8524632859 10 123009068 123009071 4 + 1.134 1.195 0.218
ENSG00000196177 E006 0.3751086 0.0315713072 1.964702e-02   10 123027534 123027541 8 + 0.000 0.369 12.466
ENSG00000196177 E007 0.8148287 0.0170302841 4.344165e-03   10 123027542 123027592 51 + 0.063 0.565 4.098
ENSG00000196177 E008 53.6711244 0.0007045796 8.892380e-02 0.2617755254 10 123034356 123034515 160 + 1.653 1.576 -0.260
ENSG00000196177 E009 54.3299428 0.0008728489 2.050027e-03 0.0173969030 10 123037747 123037847 101 + 1.671 1.501 -0.579
ENSG00000196177 E010 92.7737734 0.0067324800 5.336212e-03 0.0363648257 10 123040466 123040672 207 + 1.895 1.753 -0.481
ENSG00000196177 E011 77.2704975 0.0005161883 5.355129e-02 0.1879946378 10 123041209 123041379 171 + 1.806 1.736 -0.238
ENSG00000196177 E012 60.3132543 0.0048449322 7.936375e-02 0.2435371156 10 123043046 123043171 126 + 1.704 1.613 -0.309
ENSG00000196177 E013 51.0885649 0.0010890400 4.972571e-02 0.1789786141 10 123044393 123044485 93 + 1.634 1.536 -0.335
ENSG00000196177 E014 47.1674906 0.0008358050 6.641520e-01 0.8124875222 10 123047209 123047298 90 + 1.584 1.584 -0.001
ENSG00000196177 E015 61.2336539 0.0013230525 7.255150e-01 0.8514518708 10 123051049 123051186 138 + 1.695 1.702 0.023
ENSG00000196177 E016 6.0626035 0.0107566132 4.818490e-02 0.1754406892 10 123052761 123053060 300 + 0.690 0.986 1.154
ENSG00000196177 E017 68.9514497 0.0006360058 6.541897e-01 0.8062041458 10 123053061 123053160 100 + 1.747 1.751 0.014
ENSG00000196177 E018 641.0019019 0.0023795788 3.639224e-06 0.0000875388 10 123053695 123058290 4596 + 2.685 2.782 0.322