ENSG00000188659

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000562097 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding processed_transcript 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 0.26418188 0.31579306 0.00000000 0.31579306 0.00000000 -5.02588398 0.06110000 0.049733333 0.00000000 -0.04973333 0.91824502 0.01868443 FALSE TRUE
ENST00000565006 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding processed_transcript 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 1.15552995 2.25507088 0.98254137 0.35125797 0.50477300 -1.19035706 0.26590833 0.510333333 0.29773333 -0.21260000 0.78160761 0.01868443   FALSE
ENST00000565501 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding processed_transcript 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 0.04982014 0.00000000 0.18141660 0.00000000 0.09100950 4.25864404 0.01421250 0.000000000 0.05593333 0.05593333 0.19088215 0.01868443 FALSE TRUE
ENST00000566205 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding protein_coding 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 0.52182018 0.19247960 0.18431220 0.19247960 0.18431220 -0.05940004 0.11651667 0.030333333 0.07486667 0.04453333 0.99562215 0.01868443   FALSE
ENST00000566861 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding protein_coding 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 0.50663418 0.90206777 0.09240288 0.39369816 0.09240288 -3.15488467 0.12380000 0.213033333 0.03753333 -0.17550000 0.37892850 0.01868443   FALSE
ENST00000569120 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding protein_coding 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 1.01494050 0.58336795 0.94661090 0.30058238 0.08746633 0.68900523 0.24997083 0.120866667 0.32050000 0.19963333 0.57910900 0.01868443   FALSE
ENST00000682753 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding protein_coding 4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 0.26351719 0.03013983 0.53086706 0.03013983 0.14529840 3.75216749 0.07055417 0.008233333 0.18733333 0.17910000 0.01868443 0.01868443 FALSE TRUE
MSTRG.11383.3 ENSG00000188659 HEK293_OSMI2_6hA HEK293_TMG_6hB SAXO2 protein_coding   4.178103 4.552402 3.012017 0.8977595 0.3295033 -0.5942817 0.19985297 0.13905094 0.00000000 0.10156568 0.00000000 -3.89773352 0.05013750 0.038066667 0.00000000 -0.03806667 0.53798631 0.01868443 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000188659 E001 2.4765638 0.531036869 2.492336e-01 4.870222e-01 15 82262810 82262810 1 + 0.308 0.668 1.828
ENSG00000188659 E002 2.9528008 0.046213934 4.725274e-02 1.731587e-01 15 82262811 82262817 7 + 0.366 0.712 1.653
ENSG00000188659 E003 3.2981697 0.155928232 1.721515e-01 3.933985e-01 15 82262818 82262825 8 + 0.424 0.746 1.467
ENSG00000188659 E004 3.9360101 0.044912899 2.050144e-01 4.359432e-01 15 82262826 82262826 1 + 0.560 0.764 0.868
ENSG00000188659 E005 5.4171337 0.005549384 5.078947e-02 1.815721e-01 15 82262827 82262843 17 + 0.665 0.901 0.943
ENSG00000188659 E006 5.6198680 0.005785851 3.594156e-02 1.440640e-01 15 82262844 82262844 1 + 0.665 0.918 1.008
ENSG00000188659 E007 8.9871039 0.004027340 6.972122e-02 2.242262e-01 15 82262845 82262932 88 + 0.898 1.070 0.637
ENSG00000188659 E008 10.3586272 0.015983787 2.118374e-01 4.438964e-01 15 82262933 82263138 206 + 0.996 1.112 0.421
ENSG00000188659 E009 3.2148104 0.007252361 9.806758e-01 9.921086e-01 15 82263139 82263143 5 + 0.633 0.619 -0.058
ENSG00000188659 E010 5.0867397 0.006315089 5.697277e-01 7.500513e-01 15 82263144 82263188 45 + 0.749 0.805 0.224
ENSG00000188659 E011 5.1692364 0.006093334 9.793548e-04 9.751391e-03 15 82263359 82263438 80 + 0.519 0.950 1.780
ENSG00000188659 E012 8.2197980 0.004232338 2.242847e-03 1.868283e-02 15 82264673 82264889 217 + 0.773 1.093 1.206
ENSG00000188659 E013 5.7905262 0.366340588 2.351241e-01 4.710554e-01 15 82265569 82265720 152 + 0.971 0.650 -1.270
ENSG00000188659 E014 2.3823837 0.034594111 5.686809e-03 3.817952e-02 15 82265721 82265748 28 + 0.722 0.213 -2.752
ENSG00000188659 E015 5.1735100 0.039142179 4.484007e-04 5.208886e-03 15 82271603 82271802 200 + 0.997 0.411 -2.502
ENSG00000188659 E016 0.8878102 0.022975622 4.131544e-01   15 82271803 82272094 292 + 0.220 0.354 0.936
ENSG00000188659 E017 27.6857676 0.005707971 2.706279e-10 1.749968e-08 15 82282119 82284930 2812 + 1.631 1.200 -1.491