ENSG00000188176

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000338859 ENSG00000188176 HEK293_OSMI2_6hA HEK293_TMG_6hB SMTNL2 protein_coding protein_coding 2.215829 2.54597 0.9723412 0.1945756 0.06751509 -1.379575 1.1178290 1.2543762 0.63838110 0.37637852 0.31925814 -0.9635119 0.4861208 0.4790667 0.62243333 0.14336667 0.8546732069 3.48565e-05 FALSE TRUE
ENST00000389313 ENSG00000188176 HEK293_OSMI2_6hA HEK293_TMG_6hB SMTNL2 protein_coding protein_coding 2.215829 2.54597 0.9723412 0.1945756 0.06751509 -1.379575 0.4032530 0.5625938 0.12674963 0.28844106 0.12674963 -2.0659750 0.2055833 0.2281667 0.14786667 -0.08030000 0.7968516497 3.48565e-05 FALSE TRUE
ENST00000572951 ENSG00000188176 HEK293_OSMI2_6hA HEK293_TMG_6hB SMTNL2 protein_coding protein_coding 2.215829 2.54597 0.9723412 0.1945756 0.06751509 -1.379575 0.2660455 0.0000000 0.15896186 0.00000000 0.15896186 4.0786257 0.1035583 0.0000000 0.18546667 0.18546667 0.7854664267 3.48565e-05 FALSE FALSE
MSTRG.13453.2 ENSG00000188176 HEK293_OSMI2_6hA HEK293_TMG_6hB SMTNL2 protein_coding   2.215829 2.54597 0.9723412 0.1945756 0.06751509 -1.379575 0.1915866 0.0000000 0.04824859 0.00000000 0.04824859 2.5422231 0.1009417 0.0000000 0.04423333 0.04423333 0.6603999497 3.48565e-05 FALSE TRUE
MSTRG.13453.4 ENSG00000188176 HEK293_OSMI2_6hA HEK293_TMG_6hB SMTNL2 protein_coding   2.215829 2.54597 0.9723412 0.1945756 0.06751509 -1.379575 0.2276347 0.7290003 0.00000000 0.06872965 0.00000000 -6.2075031 0.1005375 0.2928000 0.00000000 -0.29280000 0.0000348565 3.48565e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000188176 E001 0.1723744 0.043104088 1.0000000000   17 4583999 4584000 2 + 0.000 0.068 8.934
ENSG00000188176 E002 0.8100271 0.016827630 0.3357163879   17 4584001 4584098 98 + 0.348 0.178 -1.275
ENSG00000188176 E003 3.4543645 0.625664960 0.9628546603 0.983400192 17 4584529 4584863 335 + 0.458 0.619 0.755
ENSG00000188176 E004 4.5345771 0.019892070 0.0009714124 0.009686915 17 4584864 4585004 141 + 0.000 0.748 13.694
ENSG00000188176 E005 0.0000000       17 4592355 4592360 6 +      
ENSG00000188176 E006 8.5289372 0.003883578 0.7640032873 0.875056291 17 4592361 4592448 88 + 0.923 0.900 -0.087
ENSG00000188176 E007 8.3509966 0.005612682 0.0118613696 0.065855866 17 4592929 4593171 243 + 1.103 0.829 -1.024
ENSG00000188176 E008 11.8696761 0.002958943 0.3101637557 0.550649614 17 4593822 4593897 76 + 1.103 1.013 -0.329
ENSG00000188176 E009 0.1779838 0.257311152 0.1209895443   17 4595066 4595144 79 + 0.210 0.000 -12.702
ENSG00000188176 E010 16.4396751 0.003347046 0.9994049607 1.000000000 17 4595145 4595327 183 + 1.144 1.162 0.068
ENSG00000188176 E011 12.5330475 0.003562917 0.9813816871 0.992485001 17 4596860 4596977 118 + 1.035 1.054 0.071
ENSG00000188176 E012 16.6800621 0.002174525 0.3019448599 0.542495128 17 4597172 4597323 152 + 1.230 1.152 -0.275
ENSG00000188176 E013 18.3157903 0.003042671 0.5988128979 0.769434937 17 4607361 4607588 228 + 1.230 1.197 -0.116
ENSG00000188176 E014 22.7825703 0.001827842 0.1286856689 0.329744372 17 4607589 4607919 331 + 1.180 1.325 0.510
ENSG00000188176 E015 19.7795858 0.002052013 0.1545232162 0.368788238 17 4607920 4608319 400 + 1.123 1.269 0.517