ENSG00000186866

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000331343 ENSG00000186866 HEK293_OSMI2_6hA HEK293_TMG_6hB POFUT2 protein_coding protein_coding 23.69575 28.49886 21.22836 1.434699 0.6921027 -0.424738 1.5509533 1.7750442 1.568729 0.49609599 0.1196658 -0.1771959 0.06623750 0.06443333 0.07430000 0.009866667 9.015693e-01 8.414068e-09 FALSE TRUE
ENST00000349485 ENSG00000186866 HEK293_OSMI2_6hA HEK293_TMG_6hB POFUT2 protein_coding protein_coding 23.69575 28.49886 21.22836 1.434699 0.6921027 -0.424738 15.1101425 19.6111314 11.983931 1.91869514 0.1619995 -0.7101036 0.63739167 0.68466667 0.56526667 -0.119400000 2.666496e-01 8.414068e-09 FALSE TRUE
ENST00000451615 ENSG00000186866 HEK293_OSMI2_6hA HEK293_TMG_6hB POFUT2 protein_coding protein_coding 23.69575 28.49886 21.22836 1.434699 0.6921027 -0.424738 0.8289878 0.4730758 1.918251 0.07816233 0.0704714 1.9969717 0.03613333 0.01653333 0.09073333 0.074200000 8.414068e-09 8.414068e-09 FALSE FALSE
ENST00000476653 ENSG00000186866 HEK293_OSMI2_6hA HEK293_TMG_6hB POFUT2 protein_coding processed_transcript 23.69575 28.49886 21.22836 1.434699 0.6921027 -0.424738 1.4509080 1.2048238 1.889416 0.13999093 0.3195762 0.6448090 0.06182500 0.04263333 0.08830000 0.045666667 1.368930e-01 8.414068e-09 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000186866 E001 2.358953 0.0109984224 2.818731e-01 5.221946e-01 21 45263928 45263928 1 - 0.595 0.438 -0.758
ENSG00000186866 E002 2.964841 0.0077061949 3.147821e-02 1.317070e-01 21 45263929 45263934 6 - 0.749 0.437 -1.409
ENSG00000186866 E003 31.648248 0.0013867882 5.030577e-01 7.028916e-01 21 45263935 45263978 44 - 1.511 1.498 -0.046
ENSG00000186866 E004 673.378083 0.0178829008 1.669031e-02 8.432448e-02 21 45263979 45265373 1395 - 2.706 2.864 0.525
ENSG00000186866 E005 87.582356 0.0023013708 9.703974e-04 9.677931e-03 21 45265374 45265399 26 - 1.806 1.988 0.613
ENSG00000186866 E006 243.957287 0.0037237853 1.964660e-01 4.251217e-01 21 45265400 45265635 236 - 2.322 2.398 0.256
ENSG00000186866 E007 25.441932 0.0646287822 5.913005e-01 7.645999e-01 21 45265636 45266021 386 - 1.413 1.402 -0.040
ENSG00000186866 E008 32.739468 0.0384739727 9.486380e-01 9.765632e-01 21 45266022 45266227 206 - 1.488 1.529 0.141
ENSG00000186866 E009 74.024091 0.0179482050 3.676248e-01 6.021175e-01 21 45266228 45267525 1298 - 1.878 1.848 -0.101
ENSG00000186866 E010 15.512152 0.0362564157 6.842285e-01 8.254498e-01 21 45267526 45267589 64 - 1.207 1.198 -0.031
ENSG00000186866 E011 151.811690 0.0006642733 7.969760e-01 8.945313e-01 21 45267590 45267697 108 - 2.142 2.180 0.127
ENSG00000186866 E012 87.568602 0.0004453969 8.732292e-01 9.377361e-01 21 45267698 45267713 16 - 1.914 1.937 0.079
ENSG00000186866 E013 77.129704 0.0006316865 6.543112e-01 8.062642e-01 21 45269839 45269840 2 - 1.867 1.878 0.036
ENSG00000186866 E014 85.834946 0.0006166758 3.778205e-01 6.104057e-01 21 45269841 45269850 10 - 1.879 1.945 0.221
ENSG00000186866 E015 179.539479 0.0017513096 1.643547e-01 3.831905e-01 21 45269851 45270019 169 - 2.191 2.267 0.256
ENSG00000186866 E016 4.420623 0.0170797940 5.483771e-01 7.351825e-01 21 45270020 45270094 75 - 0.640 0.757 0.490
ENSG00000186866 E017 185.722038 0.0007437260 4.262893e-01 6.482479e-01 21 45277017 45277142 126 - 2.252 2.260 0.026
ENSG00000186866 E018 14.251371 0.1919572239 2.409327e-01 4.776325e-01 21 45277233 45277773 541 - 1.264 1.100 -0.583
ENSG00000186866 E019 172.259153 0.0006381367 1.591441e-03 1.426267e-02 21 45278103 45278169 67 - 2.261 2.200 -0.203
ENSG00000186866 E020 129.253806 0.0030398126 8.639839e-03 5.222162e-02 21 45282349 45282368 20 - 2.144 2.070 -0.250
ENSG00000186866 E021 117.667275 0.0020625772 1.698717e-02 8.541770e-02 21 45282369 45282398 30 - 2.096 2.034 -0.208
ENSG00000186866 E022 142.631886 0.0003384436 1.069585e-04 1.582369e-03 21 45282399 45282459 61 - 2.193 2.106 -0.292
ENSG00000186866 E023 13.064116 0.0028323118 2.433571e-07 8.195050e-06 21 45282804 45282938 135 - 1.358 0.901 -1.650
ENSG00000186866 E024 10.703165 0.0600473848 8.930893e-01 9.481933e-01 21 45283352 45283382 31 - 1.056 1.058 0.009
ENSG00000186866 E025 210.202955 0.0002763850 1.365277e-02 7.297708e-02 21 45283383 45283527 145 - 2.327 2.295 -0.107
ENSG00000186866 E026 5.528206 0.0047045662 1.845708e-02 9.056189e-02 21 45285269 45285328 60 - 0.953 0.674 -1.100
ENSG00000186866 E027 9.730971 0.0048494972 1.433464e-02 7.563855e-02 21 45285483 45285677 195 - 1.147 0.913 -0.857
ENSG00000186866 E028 230.733750 0.0072190739 2.408233e-01 4.775217e-01 21 45285678 45285928 251 - 2.363 2.337 -0.084
ENSG00000186866 E029 86.701794 0.0128030873 9.743216e-01 9.890505e-01 21 45287741 45287898 158 - 1.906 1.932 0.089