ENSG00000186687

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379380 ENSG00000186687 HEK293_OSMI2_6hA HEK293_TMG_6hB LYRM7 protein_coding protein_coding 6.210324 1.964027 10.02289 0.1139836 0.1086004 2.345523 5.663046 1.3223393 9.693779 0.02652483 0.2623421 2.864585 0.7947250 0.6769333 0.9668333 0.2899000 5.794670e-05 8.416689e-18 FALSE FALSE
ENST00000521227 ENSG00000186687 HEK293_OSMI2_6hA HEK293_TMG_6hB LYRM7 protein_coding processed_transcript 6.210324 1.964027 10.02289 0.1139836 0.1086004 2.345523 0.374483 0.6221056 0.000000 0.09865606 0.0000000 -5.982094 0.1800708 0.3132667 0.0000000 -0.3132667 8.416689e-18 8.416689e-18   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000186687 E001 11.26899 0.0028777083 1.724405e-01 3.937581e-01 5 131170944 131170988 45 + 0.990 0.801 -0.720
ENSG00000186687 E002 28.04429 0.0030714419 5.012647e-01 7.016846e-01 5 131170989 131171038 50 + 1.341 1.284 -0.198
ENSG00000186687 E003 38.68190 0.0009650337 6.482732e-01 8.023650e-01 5 131180095 131180167 73 + 1.470 1.441 -0.099
ENSG00000186687 E004 47.95766 0.0008495501 8.261476e-02 2.499445e-01 5 131182229 131182299 71 + 1.572 1.453 -0.408
ENSG00000186687 E005 45.99651 0.0009598148 1.105950e-01 2.999678e-01 5 131187028 131187109 82 + 1.552 1.441 -0.381
ENSG00000186687 E006 365.23547 0.0002609851 3.837460e-04 4.576789e-03 5 131199531 131202639 3109 + 2.435 2.364 -0.238
ENSG00000186687 E007 49.49615 0.0007448260 9.864457e-01 9.950649e-01 5 131202640 131202813 174 + 1.567 1.574 0.024
ENSG00000186687 E008 163.69084 0.0003393234 7.210077e-01 8.488083e-01 5 131202814 131203590 777 + 2.079 2.072 -0.022
ENSG00000186687 E009 262.92189 0.0009590037 5.022140e-09 2.524045e-07 5 131203591 131205428 1838 + 2.254 2.410 0.521