ENSG00000186432

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334256 ENSG00000186432 HEK293_OSMI2_6hA HEK293_TMG_6hB KPNA4 protein_coding protein_coding 23.38246 8.501563 38.11633 2.110588 1.641326 2.163292 1.945770 1.3414600 2.673877 0.3399574 0.2227528 0.9897995 0.1133708 0.16130000 0.07006667 -0.09123333 0.01569206 0.01569206 FALSE TRUE
ENST00000483437 ENSG00000186432 HEK293_OSMI2_6hA HEK293_TMG_6hB KPNA4 protein_coding protein_coding 23.38246 8.501563 38.11633 2.110588 1.641326 2.163292 5.521719 0.3568828 10.659040 0.1788946 1.0211722 4.8619674 0.1543250 0.05806667 0.27863333 0.22056667 0.25171849 0.01569206 FALSE TRUE
ENST00000676799 ENSG00000186432 HEK293_OSMI2_6hA HEK293_TMG_6hB KPNA4 protein_coding nonsense_mediated_decay 23.38246 8.501563 38.11633 2.110588 1.641326 2.163292 8.442381 3.8941474 12.858202 1.2226276 0.9747793 1.7207310 0.3996000 0.44133333 0.33896667 -0.10236667 0.69245684 0.01569206 FALSE TRUE
ENST00000678765 ENSG00000186432 HEK293_OSMI2_6hA HEK293_TMG_6hB KPNA4 protein_coding protein_coding 23.38246 8.501563 38.11633 2.110588 1.641326 2.163292 6.288979 2.6138483 10.526764 1.0693439 0.4946608 2.0056757 0.2717792 0.28776667 0.27613333 -0.01163333 1.00000000 0.01569206 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000186432 E001 241.8271967 0.0124240787 8.296690e-07 2.412205e-05 3 160495007 160500172 5166 - 2.217 2.518 1.003
ENSG00000186432 E002 9.9657400 0.0031184339 1.097136e-04 1.615249e-03 3 160500173 160500192 20 - 0.832 1.271 1.608
ENSG00000186432 E003 28.8249543 0.0018532208 5.818011e-06 1.315097e-04 3 160500193 160500208 16 - 1.296 1.640 1.185
ENSG00000186432 E004 804.6686193 0.0014559944 1.553978e-07 5.508270e-06 3 160500209 160502039 1831 - 2.794 2.926 0.441
ENSG00000186432 E005 152.0929975 0.0012898372 6.746200e-02 2.193723e-01 3 160502040 160502202 163 - 2.114 2.076 -0.129
ENSG00000186432 E006 0.5530924 0.0337203539 1.099790e-01   3 160504001 160504067 67 - 0.068 0.347 2.841
ENSG00000186432 E007 167.9513665 0.0007033580 1.400993e-01 3.476720e-01 3 160504958 160505052 95 - 2.153 2.134 -0.061
ENSG00000186432 E008 2.5463882 0.0088776160 6.807611e-01 8.231863e-01 3 160505053 160507354 2302 - 0.508 0.452 -0.278
ENSG00000186432 E009 206.4847431 0.0003151164 6.596269e-01 8.097613e-01 3 160508107 160508269 163 - 2.235 2.254 0.063
ENSG00000186432 E010 148.3254334 0.0004240386 9.148811e-01 9.595934e-01 3 160509800 160509871 72 - 2.091 2.126 0.119
ENSG00000186432 E011 1.4570612 0.0123970655 3.690268e-01 6.032444e-01 3 160513250 160514076 827 - 0.374 0.207 -1.162
ENSG00000186432 E012 162.6537290 0.0002710383 5.174312e-02 1.838513e-01 3 160514077 160514181 105 - 2.142 2.109 -0.112
ENSG00000186432 E013 190.2696274 0.0002722118 3.553529e-04 4.289919e-03 3 160515452 160515580 129 - 2.218 2.137 -0.273
ENSG00000186432 E014 201.6175351 0.0002534562 6.081329e-03 4.013171e-02 3 160521779 160521910 132 - 2.238 2.186 -0.173
ENSG00000186432 E015 0.4820342 0.0213142014 5.191991e-01   3 160521911 160522861 951 - 0.180 0.001 -8.299
ENSG00000186432 E016 0.3150090 0.0415841104 3.711467e-01   3 160525021 160525132 112 - 0.068 0.207 1.842
ENSG00000186432 E017 127.4273586 0.0003257113 7.353048e-02 2.318320e-01 3 160525800 160525844 45 - 2.038 2.002 -0.122
ENSG00000186432 E018 189.9172293 0.0010860235 3.905639e-02 1.523422e-01 3 160525938 160526107 170 - 2.213 2.173 -0.133
ENSG00000186432 E019 132.9537894 0.0020250470 1.630407e-01 3.812627e-01 3 160527953 160528039 87 - 2.057 2.031 -0.089
ENSG00000186432 E020 143.0817773 0.0003349472 2.628870e-02 1.161703e-01 3 160530855 160530940 86 - 2.092 2.044 -0.161
ENSG00000186432 E021 158.0204601 0.0003409062 1.247025e-04 1.795604e-03 3 160531462 160531557 96 - 2.145 2.042 -0.346
ENSG00000186432 E022 128.8095963 0.0003595547 8.802172e-04 8.931712e-03 3 160535513 160535565 53 - 2.057 1.960 -0.324
ENSG00000186432 E023 102.9968630 0.0004392556 3.405012e-07 1.104317e-05 3 160535661 160535690 30 - 1.978 1.779 -0.670
ENSG00000186432 E024 136.4399043 0.0082677926 6.175342e-02 2.067738e-01 3 160535808 160535897 90 - 2.079 1.996 -0.279
ENSG00000186432 E025 101.5446439 0.0005476362 3.362298e-02 1.378317e-01 3 160536796 160536840 45 - 1.948 1.887 -0.203
ENSG00000186432 E026 2.1068900 0.0095665790 1.877271e-02 9.163396e-02 3 160564209 160564504 296 - 0.306 0.722 2.060
ENSG00000186432 E027 1.4171323 0.0127181000 6.423290e-03 4.179236e-02 3 160564505 160564520 16 - 0.179 0.668 2.839
ENSG00000186432 E028 3.3989358 0.0169519678 5.604485e-05 9.196594e-04 3 160564521 160564640 120 - 0.341 0.980 2.843
ENSG00000186432 E029 107.8817186 0.0005460107 7.424113e-01 8.619044e-01 3 160565214 160565571 358 - 1.959 1.977 0.061