ENSG00000185728

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000517371 ENSG00000185728 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF3 protein_coding protein_coding 18.47482 3.281414 31.85402 0.9016178 1.968 3.275149 0.5955368 0.0000000 0.03836183 0.0000000 0.01922516 2.2738687 0.1123333 0.0000000 0.001266667 0.001266667 1.000000e+00 3.457498e-07   FALSE
ENST00000522282 ENSG00000185728 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF3 protein_coding retained_intron 18.47482 3.281414 31.85402 0.9016178 1.968 3.275149 2.6749910 0.0000000 4.28843253 0.0000000 1.29201350 8.7476669 0.0797125 0.0000000 0.131000000 0.131000000 2.567760e-05 3.457498e-07   FALSE
ENST00000539294 ENSG00000185728 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF3 protein_coding protein_coding 18.47482 3.281414 31.85402 0.9016178 1.968 3.275149 10.8537063 2.6937845 20.64242463 0.9680488 0.69440309 2.9332591 0.5584583 0.7614667 0.650433333 -0.111033333 7.656238e-01 3.457498e-07 FALSE TRUE
ENST00000617200 ENSG00000185728 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF3 protein_coding protein_coding 18.47482 3.281414 31.85402 0.9016178 1.968 3.275149 0.4178501 0.3822298 0.51712185 0.1927107 0.14070761 0.4264375 0.0601750 0.1799667 0.016366667 -0.163600000 5.998568e-01 3.457498e-07 FALSE TRUE
ENST00000619337 ENSG00000185728 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF3 protein_coding protein_coding 18.47482 3.281414 31.85402 0.9016178 1.968 3.275149 2.1155879 0.0000000 4.92401722 0.0000000 1.01178913 8.9466189 0.0625375 0.0000000 0.153433333 0.153433333 3.457498e-07 3.457498e-07 FALSE TRUE
ENST00000621890 ENSG00000185728 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF3 protein_coding protein_coding 18.47482 3.281414 31.85402 0.9016178 1.968 3.275149 0.1580720 0.0000000 0.00000000 0.0000000 0.00000000 0.0000000 0.0525500 0.0000000 0.000000000 0.000000000   3.457498e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000185728 E001 0.6316833 0.0360223817 0.61911464   8 63167948 63168552 605 + 0.138 0.263 1.147
ENSG00000185728 E002 0.4514866 0.0217681645 0.41562919   8 63168553 63168561 9 + 0.097 0.263 1.736
ENSG00000185728 E003 9.8416667 0.0054179839 0.46451735 0.67624328 8 63168562 63168620 59 + 0.867 1.041 0.652
ENSG00000185728 E004 29.1593226 0.0394250852 0.80406273 0.89863316 8 63168621 63168651 31 + 1.312 1.368 0.195
ENSG00000185728 E005 34.7326124 0.0322306403 0.32661247 0.56579799 8 63168652 63168653 2 + 1.396 1.369 -0.095
ENSG00000185728 E006 35.9753451 0.0329312732 0.56317406 0.74553345 8 63168654 63168654 1 + 1.404 1.426 0.075
ENSG00000185728 E007 39.5748561 0.0335496402 0.48743754 0.69224038 8 63168655 63168660 6 + 1.445 1.454 0.029
ENSG00000185728 E008 45.7095566 0.0350935116 0.41333275 0.63860552 8 63168661 63168686 26 + 1.507 1.509 0.008
ENSG00000185728 E009 78.1497360 0.0262093952 0.40863880 0.63498357 8 63168687 63168814 128 + 1.731 1.756 0.086
ENSG00000185728 E010 66.4408075 0.0276827689 0.03440457 0.13997827 8 63168815 63168901 87 + 1.678 1.566 -0.381
ENSG00000185728 E011 0.0000000       8 63168961 63169002 42 +      
ENSG00000185728 E012 0.0000000       8 63169003 63169008 6 +      
ENSG00000185728 E013 0.1614157 0.0340798527 1.00000000   8 63169009 63169019 11 + 0.051 0.000 -14.228
ENSG00000185728 E014 0.1614157 0.0340798527 1.00000000   8 63169020 63169021 2 + 0.051 0.000 -14.228
ENSG00000185728 E015 0.1614157 0.0340798527 1.00000000   8 63169022 63169105 84 + 0.051 0.000 -14.228
ENSG00000185728 E016 0.0000000       8 63169106 63169111 6 +      
ENSG00000185728 E017 46.7357179 0.0356493723 0.08404876 0.25286846 8 63169387 63169388 2 + 1.528 1.426 -0.349
ENSG00000185728 E018 54.5329127 0.0198117458 0.03440373 0.13997827 8 63169389 63169411 23 + 1.594 1.489 -0.359
ENSG00000185728 E019 0.6622309 0.2076978779 0.71249645   8 63169412 63169491 80 + 0.177 0.000 -16.241
ENSG00000185728 E020 3.1405095 0.0107556951 0.60443400 0.77317989 8 63172653 63172798 146 + 0.477 0.637 0.738
ENSG00000185728 E021 0.4929928 0.0297199314 0.41070284   8 63173651 63173681 31 + 0.097 0.263 1.740
ENSG00000185728 E022 42.7426493 0.0089235601 0.05214009 0.18473224 8 63175331 63175336 6 + 1.490 1.414 -0.263
ENSG00000185728 E023 73.8091885 0.0167101951 0.01003153 0.05829268 8 63175337 63175416 80 + 1.725 1.601 -0.419
ENSG00000185728 E024 0.5963342 0.0217681645 0.60803863   8 63175417 63175700 284 + 0.138 0.263 1.152
ENSG00000185728 E025 586.3080543 0.0589531571 0.05101211 0.18209207 8 63186147 63187529 1383 + 2.609 2.548 -0.203
ENSG00000185728 E026 117.4156550 0.0005209747 0.96093374 0.98240967 8 63187530 63187745 216 + 1.904 1.989 0.286
ENSG00000185728 E027 0.3337900 0.0576305715 0.21504385   8 63188735 63189042 308 + 0.051 0.264 2.740
ENSG00000185728 E028 632.7729383 1.9947822706 0.45518864 0.66962813 8 63209683 63212786 3104 + 2.583 2.871 0.960