ENSG00000185716

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000542527 ENSG00000185716 HEK293_OSMI2_6hA HEK293_TMG_6hB MOSMO protein_coding protein_coding 7.914327 4.283498 10.4907 0.2816092 0.6102199 1.290259 2.6770548 0.8416524 4.805437 0.02419056 0.42224270 2.499330 0.29682500 0.19900000 0.4565000 0.2575000 7.170561e-05 3.874718e-05 FALSE TRUE
ENST00000562695 ENSG00000185716 HEK293_OSMI2_6hA HEK293_TMG_6hB MOSMO protein_coding protein_coding 7.914327 4.283498 10.4907 0.2816092 0.6102199 1.290259 0.6762805 0.2556820 1.379081 0.05821836 0.08321749 2.386359 0.07881667 0.06203333 0.1315333 0.0695000 2.508841e-01 3.874718e-05 FALSE TRUE
MSTRG.12254.6 ENSG00000185716 HEK293_OSMI2_6hA HEK293_TMG_6hB MOSMO protein_coding   7.914327 4.283498 10.4907 0.2816092 0.6102199 1.290259 3.9548223 2.9902824 3.734129 0.33622183 0.27181047 0.319532 0.55479167 0.69410000 0.3556667 -0.3384333 3.874718e-05 3.874718e-05 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000185716 E001 0.0000000       16 22007638 22007696 59 +      
ENSG00000185716 E002 0.0000000       16 22008111 22008208 98 +      
ENSG00000185716 E003 0.3503582 0.0300748470 4.477279e-01   16 22008209 22008282 74 + 0.072 0.198 1.678
ENSG00000185716 E004 3.4021756 0.0191616617 8.199929e-01 9.080596e-01 16 22008283 22008310 28 + 0.569 0.650 0.354
ENSG00000185716 E005 34.6987272 0.0011631812 2.377057e-03 1.954956e-02 16 22008311 22008407 97 + 1.416 1.653 0.811
ENSG00000185716 E006 0.5657866 0.0325257147 3.156250e-01   16 22064338 22064438 101 + 0.134 0.333 1.677
ENSG00000185716 E007 0.6238610 0.0197987629 3.124706e-01   16 22075294 22075486 193 + 0.134 0.333 1.681
ENSG00000185716 E008 69.6179700 0.0008789882 1.278182e-02 6.955421e-02 16 22075487 22075699 213 + 1.738 1.898 0.541
ENSG00000185716 E009 13.7956514 0.0028000010 9.565221e-02 2.741779e-01 16 22075700 22076378 679 + 1.140 1.010 -0.469
ENSG00000185716 E010 229.8245247 0.0010739091 4.283656e-29 3.190629e-26 16 22080696 22083675 2980 + 2.341 2.125 -0.723
ENSG00000185716 E011 21.2863037 0.0023519555 5.836503e-01 7.593725e-01 16 22083676 22083868 193 + 1.287 1.290 0.011
ENSG00000185716 E012 45.7618105 0.0008115315 6.220591e-01 7.852012e-01 16 22083869 22084654 786 + 1.585 1.664 0.267
ENSG00000185716 E013 0.6294705 0.0511692517 1.000000e+00   16 22085131 22085207 77 + 0.188 0.197 0.089
ENSG00000185716 E014 3.1704916 0.1232852737 2.795204e-01 5.196274e-01 16 22085208 22085275 68 + 0.516 0.746 1.001
ENSG00000185716 E015 24.9759722 0.0293428508 8.275547e-05 1.277849e-03 16 22085644 22085750 107 + 1.197 1.643 1.547
ENSG00000185716 E016 40.1108641 0.1663142588 1.708497e-03 1.507388e-02 16 22087057 22087534 478 + 1.313 1.913 2.047