ENSG00000185305

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000504924 ENSG00000185305 HEK293_OSMI2_6hA HEK293_TMG_6hB ARL15 protein_coding protein_coding 6.384931 1.22582 12.85852 0.2923481 0.2773795 3.380305 4.6776792 1.22582 9.207080 0.2923481 0.07925597 2.898841 0.8220542 1 0.7169667 -0.2830333 3.350693e-08 3.350693e-08 FALSE TRUE
ENST00000507646 ENSG00000185305 HEK293_OSMI2_6hA HEK293_TMG_6hB ARL15 protein_coding protein_coding 6.384931 1.22582 12.85852 0.2923481 0.2773795 3.380305 0.9401352 0.00000 2.598775 0.0000000 0.02748080 8.027228 0.0852625 0 0.2023333 0.2023333 6.177083e-07 3.350693e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000185305 E001 1.4874211 0.0122827461 1.000000e+00 1.000000e+00 5 53883942 53883944 3 - 0.291 0.273 -0.129
ENSG00000185305 E002 318.6023049 0.0006990308 4.417975e-07 1.389653e-05 5 53883945 53886444 2500 - 2.309 2.407 0.328
ENSG00000185305 E003 30.2817741 0.0012113517 9.977497e-01 1.000000e+00 5 53886445 53886521 77 - 1.318 1.324 0.019
ENSG00000185305 E004 47.8771048 0.0010465635 9.394782e-01 9.719602e-01 5 53886522 53886713 192 - 1.510 1.511 0.002
ENSG00000185305 E005 0.0000000       5 53887365 53887383 19 -      
ENSG00000185305 E006 0.7381610 0.0190175092 1.192662e-02   5 54113133 54113201 69 - 0.093 0.558 3.453
ENSG00000185305 E007 52.3888451 0.0007506822 3.216409e-03 2.470407e-02 5 54113202 54113410 209 - 1.570 1.341 -0.788
ENSG00000185305 E008 0.8855476 0.9215462802 5.126661e-01   5 54125412 54125477 66 - 0.193 0.276 0.669
ENSG00000185305 E009 0.0000000       5 54154391 54154579 189 -      
ENSG00000185305 E010 35.7662133 0.0012416818 1.498452e-02 7.802527e-02 5 54154580 54154639 60 - 1.406 1.175 -0.809
ENSG00000185305 E011 44.6664577 0.0009430702 5.122314e-03 3.525377e-02 5 54171784 54171928 145 - 1.504 1.266 -0.824
ENSG00000185305 E012 0.9287192 0.0165272808 5.317056e-01   5 54282271 54282327 57 - 0.234 0.000 -9.489
ENSG00000185305 E013 1.1442650 0.0271901639 4.278933e-01   5 54307888 54307946 59 - 0.264 0.000 -9.584
ENSG00000185305 E014 0.6434498 0.0196015304 1.000000e+00   5 54310295 54310431 137 - 0.169 0.000 -8.912
ENSG00000185305 E015 23.7049526 0.0018936864 1.214872e-02 6.705663e-02 5 54310432 54310582 151 - 1.248 0.947 -1.089