ENSG00000185246

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355765 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding protein_coding 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 2.7608357 0.8769381 4.722083 0.45826938 1.05717075 2.415570 0.15194167 0.18670000 0.11783333 -0.06886667 1.000000e+00 1.053958e-08 FALSE TRUE
ENST00000424478 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding nonsense_mediated_decay 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 1.7643362 0.2748787 3.630855 0.14472598 0.16860945 3.675858 0.08248333 0.05403333 0.09256667 0.03853333 7.289125e-01 1.053958e-08 FALSE TRUE
ENST00000477626 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding retained_intron 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 4.4814205 1.1001176 9.618248 0.34664218 0.04419354 3.116561 0.20469167 0.23126667 0.24456667 0.01330000 9.650730e-01 1.053958e-08 FALSE TRUE
ENST00000553605 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding protein_coding 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 1.8966790 0.0000000 2.553785 0.00000000 1.66503900 8.002131 0.05429583 0.00000000 0.06066667 0.06066667 5.963074e-01 1.053958e-08 FALSE FALSE
ENST00000554081 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding nonsense_mediated_decay 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 0.2703865 0.8072247 0.000000 0.26088188 0.00000000 -6.352661 0.05905417 0.17940000 0.00000000 -0.17940000 1.053958e-08 1.053958e-08 FALSE TRUE
ENST00000554785 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding retained_intron 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 1.6833945 0.5117986 3.273943 0.03204022 0.29397763 2.653864 0.10270000 0.11016667 0.08386667 -0.02630000 7.638495e-01 1.053958e-08 FALSE TRUE
ENST00000556718 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding retained_intron 20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 1.8470949 0.2011845 3.547286 0.20118446 1.05554653 4.074201 0.07180000 0.04290000 0.08750000 0.04460000 5.187302e-01 1.053958e-08 FALSE FALSE
MSTRG.9459.1 ENSG00000185246 HEK293_OSMI2_6hA HEK293_TMG_6hB PRPF39 protein_coding   20.3731 4.730631 39.54777 0.6147202 2.123657 3.06081 2.5156143 0.5755942 6.012543 0.30537978 1.68649700 3.362400 0.12061250 0.11330000 0.15550000 0.04220000 8.699934e-01 1.053958e-08 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000185246 E001 0.0000000       14 45084076 45084088 13 +      
ENSG00000185246 E002 2.0926887 0.0116801241 0.9204717444 0.962475752 14 45084089 45084106 18 + 0.388 0.413 0.136
ENSG00000185246 E003 3.8969717 0.0065941378 0.3687022961 0.602969194 14 45084107 45084115 9 + 0.590 0.413 -0.866
ENSG00000185246 E004 4.0396064 0.0062524191 0.3309370183 0.569836670 14 45084116 45084117 2 + 0.604 0.413 -0.930
ENSG00000185246 E005 8.1411653 0.0066782217 0.0649115346 0.213701620 14 45084118 45084127 10 + 0.856 0.529 -1.377
ENSG00000185246 E006 79.1838534 0.0004691678 0.0408386401 0.156905470 14 45084128 45084249 122 + 1.770 1.657 -0.384
ENSG00000185246 E007 69.6194865 0.0006425463 0.1204554228 0.316561092 14 45095221 45095238 18 + 1.714 1.625 -0.303
ENSG00000185246 E008 99.3828948 0.0005484479 0.1031143399 0.287384284 14 45095239 45095305 67 + 1.865 1.787 -0.262
ENSG00000185246 E009 52.3637724 0.0007595328 0.1041008748 0.288967176 14 45095306 45095308 3 + 1.592 1.482 -0.377
ENSG00000185246 E010 106.2140164 0.0003758847 0.0126374268 0.068993866 14 45095309 45095405 97 + 1.896 1.776 -0.407
ENSG00000185246 E011 89.0692546 0.0004386021 0.0396155102 0.153748993 14 45095406 45095445 40 + 1.820 1.714 -0.361
ENSG00000185246 E012 107.6478732 0.0003826845 0.0377167693 0.148879949 14 45095446 45095533 88 + 1.900 1.803 -0.327
ENSG00000185246 E013 56.0479865 0.0007486354 0.2187715691 0.451926726 14 45095534 45095563 30 + 1.621 1.545 -0.261
ENSG00000185246 E014 0.5941213 0.0212151477 0.5582367152   14 45095564 45095733 170 + 0.144 0.253 1.009
ENSG00000185246 E015 98.1348704 0.0005646530 0.0035272464 0.026505932 14 45096103 45096197 95 + 1.866 1.714 -0.513
ENSG00000185246 E016 85.9455740 0.0042711617 0.0298363488 0.126846871 14 45096198 45096228 31 + 1.806 1.671 -0.457
ENSG00000185246 E017 47.8566095 0.0207657819 0.5100224504 0.707987382 14 45096229 45096423 195 + 1.547 1.492 -0.191
ENSG00000185246 E018 40.2666730 0.0126884510 0.4294353119 0.650596758 14 45096424 45096492 69 + 1.476 1.408 -0.233
ENSG00000185246 E019 52.5963045 0.0519522787 0.8710137854 0.936499190 14 45096493 45096595 103 + 1.579 1.581 0.008
ENSG00000185246 E020 53.6998642 0.0096534081 0.8059326012 0.899675879 14 45096596 45096648 53 + 1.591 1.573 -0.061
ENSG00000185246 E021 68.6838759 0.0094323787 0.9187098742 0.961543141 14 45096649 45096758 110 + 1.692 1.701 0.032
ENSG00000185246 E022 40.0128319 0.0506364865 0.4628704880 0.675056187 14 45096759 45096886 128 + 1.475 1.408 -0.229
ENSG00000185246 E023 77.6107986 0.0005692123 0.4595038999 0.672779346 14 45096887 45096905 19 + 1.749 1.714 -0.121
ENSG00000185246 E024 127.2700398 0.0007587013 0.0961911338 0.275081957 14 45096906 45097005 100 + 1.967 1.896 -0.240
ENSG00000185246 E025 146.3512734 0.0003909525 0.0590335483 0.200585319 14 45102529 45102696 168 + 2.028 1.953 -0.250
ENSG00000185246 E026 179.7188832 0.0003891206 0.8319614357 0.914971356 14 45107451 45107616 166 + 2.106 2.104 -0.007
ENSG00000185246 E027 176.5176409 0.0006266736 0.4297799827 0.650869258 14 45108415 45108522 108 + 2.094 2.130 0.119
ENSG00000185246 E028 160.7071811 0.0008694427 0.2460465785 0.483697541 14 45109616 45109731 116 + 2.052 2.103 0.173
ENSG00000185246 E029 75.1262165 0.0006926067 0.7819623337 0.885797309 14 45109732 45109742 11 + 1.730 1.752 0.075
ENSG00000185246 E030 98.6322430 0.0005278836 0.9623118895 0.983143475 14 45109743 45109780 38 + 1.846 1.850 0.013
ENSG00000185246 E031 36.1263421 0.0010109001 0.2183881678 0.451480703 14 45109781 45109893 113 + 1.433 1.335 -0.342
ENSG00000185246 E032 77.7205631 0.0005248238 0.1287456255 0.329825281 14 45109894 45109993 100 + 1.732 1.819 0.296
ENSG00000185246 E033 32.1589212 0.0031435663 0.5939478715 0.766297471 14 45109994 45110070 77 + 1.378 1.335 -0.149
ENSG00000185246 E034 23.2636959 0.0015791218 0.6602619631 0.810124179 14 45110071 45110093 23 + 1.244 1.206 -0.135
ENSG00000185246 E035 160.8983352 0.0003060946 0.1729320529 0.394378611 14 45110094 45110220 127 + 2.049 2.107 0.194
ENSG00000185246 E036 119.7036355 0.0005513438 0.4349631063 0.654948870 14 45110549 45110602 54 + 1.923 1.964 0.139
ENSG00000185246 E037 93.0305440 0.0003997744 0.0405730576 0.156244776 14 45110603 45110628 26 + 1.807 1.913 0.356
ENSG00000185246 E038 211.6737451 0.0002242140 0.0015763242 0.014152583 14 45110629 45110817 189 + 2.160 2.267 0.358
ENSG00000185246 E039 230.5929726 0.0003457228 0.0221872344 0.103230211 14 45112318 45112502 185 + 2.203 2.281 0.262
ENSG00000185246 E040 140.6378716 0.0003976208 0.5364380472 0.726628979 14 45114183 45114257 75 + 1.998 2.030 0.108
ENSG00000185246 E041 207.0407360 0.0003276558 0.4768854358 0.684887997 14 45114494 45114614 121 + 2.172 2.155 -0.059
ENSG00000185246 E042 13.6969943 0.0025695511 0.1211189071 0.317642832 14 45114615 45114856 242 + 1.002 1.183 0.654
ENSG00000185246 E043 331.9900337 0.0048050853 0.0009902158 0.009837453 14 45114857 45116282 1426 + 2.351 2.482 0.438