ENSG00000185238

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330796 ENSG00000185238 HEK293_OSMI2_6hA HEK293_TMG_6hB PRMT3 protein_coding nonsense_mediated_decay 20.83319 4.437067 34.47618 1.037066 1.290269 2.955092 1.245756 0.7349955 1.016603 0.1319720 0.1020759 0.4625753 0.05964167 0.1714667 0.02940000 -0.142066667 7.560794e-10 7.560794e-10 FALSE TRUE
ENST00000331079 ENSG00000185238 HEK293_OSMI2_6hA HEK293_TMG_6hB PRMT3 protein_coding protein_coding 20.83319 4.437067 34.47618 1.037066 1.290269 2.955092 13.132146 2.3116615 24.067380 0.8006472 1.4259577 3.3744489 0.62733333 0.4830667 0.69693333 0.213866667 3.939885e-01 7.560794e-10 FALSE TRUE
ENST00000526583 ENSG00000185238 HEK293_OSMI2_6hA HEK293_TMG_6hB PRMT3 protein_coding nonsense_mediated_decay 20.83319 4.437067 34.47618 1.037066 1.290269 2.955092 1.341677 0.2318537 2.073360 0.0724666 0.2527819 3.1067052 0.05982083 0.0550000 0.06083333 0.005833333 9.811064e-01 7.560794e-10 FALSE FALSE
MSTRG.5286.2 ENSG00000185238 HEK293_OSMI2_6hA HEK293_TMG_6hB PRMT3 protein_coding   20.83319 4.437067 34.47618 1.037066 1.290269 2.955092 4.507284 1.1341865 6.165552 0.2229192 0.4657258 2.4322458 0.21177917 0.2833667 0.17986667 -0.103500000 6.709427e-01 7.560794e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000185238 E001 0.1426347 0.0330693198 8.906500e-01   11 20387558 20387558 1 + 0.052 0.001 -5.826
ENSG00000185238 E002 8.1359979 0.0172691772 1.145602e-05 0.0002355607 11 20387559 20387639 81 + 0.686 1.255 2.141
ENSG00000185238 E003 23.9288705 0.0083959090 2.147328e-03 0.0180613875 11 20387640 20387715 76 + 1.191 1.479 1.003
ENSG00000185238 E004 39.3350516 0.0016638468 2.110467e-02 0.0996817626 11 20387716 20387746 31 + 1.423 1.593 0.582
ENSG00000185238 E005 49.1430019 0.0040395045 5.327749e-01 0.7240478139 11 20387747 20387774 28 + 1.539 1.584 0.153
ENSG00000185238 E006 8.6033056 0.0035639959 8.769676e-01 0.9397172734 11 20387775 20388018 244 + 0.847 0.825 -0.084
ENSG00000185238 E007 85.1666822 0.0004789844 2.021980e-01 0.4323722146 11 20388019 20388154 136 + 1.787 1.717 -0.235
ENSG00000185238 E008 81.4121217 0.0007427319 5.264285e-03 0.0360032455 11 20389744 20389826 83 + 1.779 1.610 -0.571
ENSG00000185238 E009 56.2247948 0.0007591325 1.966596e-01 0.4253784949 11 20392211 20392260 50 + 1.613 1.525 -0.299
ENSG00000185238 E010 85.0253751 0.0005253585 1.607291e-01 0.3778604604 11 20392897 20392999 103 + 1.788 1.711 -0.261
ENSG00000185238 E011 0.3393995 0.0245941750 1.000000e+00   11 20393791 20393854 64 + 0.099 0.000 -8.883
ENSG00000185238 E012 129.7936376 0.0028580291 3.147517e-01 0.5549116005 11 20395803 20395962 160 + 1.954 2.003 0.165
ENSG00000185238 E013 156.5426419 0.0003273917 6.636497e-01 0.8121859729 11 20397577 20397721 145 + 2.043 2.028 -0.052
ENSG00000185238 E014 127.1793510 0.0004861219 9.295501e-01 0.9667980228 11 20402919 20402984 66 + 1.950 1.948 -0.006
ENSG00000185238 E015 7.7173305 0.0044347396 8.966074e-01 0.9501392571 11 20405446 20407910 2465 + 0.805 0.825 0.081
ENSG00000185238 E016 149.9494650 0.0010270517 6.871176e-01 0.8272313680 11 20407911 20408032 122 + 2.022 2.007 -0.050
ENSG00000185238 E017 130.1401244 0.0004033652 7.108525e-01 0.8423648156 11 20426766 20426865 100 + 1.962 1.948 -0.047
ENSG00000185238 E018 130.7096569 0.0006261119 2.066377e-01 0.4376816080 11 20452130 20452208 79 + 1.967 1.911 -0.189
ENSG00000185238 E019 178.0991120 0.0002916686 5.612012e-01 0.7441172540 11 20461980 20462167 188 + 2.097 2.078 -0.066
ENSG00000185238 E020 96.4154346 0.0014756353 2.688277e-01 0.5085735496 11 20464460 20464546 87 + 1.843 1.785 -0.193
ENSG00000185238 E021 81.9641508 0.0041754813 9.461025e-01 0.9753948245 11 20493919 20493969 51 + 1.765 1.772 0.025
ENSG00000185238 E022 124.1905228 0.0006221651 2.440710e-01 0.4811277644 11 20494167 20494254 88 + 1.949 1.897 -0.175
ENSG00000185238 E023 419.6657789 0.0001779009 7.338337e-03 0.0462676249 11 20508304 20509338 1035 + 2.458 2.518 0.202