ENSG00000185163

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329073 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding retained_intron 38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 2.393240 2.704323 2.4320113 0.5874744 0.4319317 -0.1525226 0.06590417 0.05973333 0.082366667 0.022633333 0.78154769 0.03014644 FALSE FALSE
ENST00000397333 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding protein_coding 38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 11.135564 12.937336 8.1595262 0.9530083 1.1188313 -0.6643310 0.29019167 0.28063333 0.276866667 -0.003766667 1.00000000 0.03014644 FALSE TRUE
ENST00000462829 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding retained_intron 38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 1.717806 1.379505 3.3840033 0.8076941 0.4349850 1.2884172 0.05045000 0.03150000 0.114433333 0.082933333 0.14941990 0.03014644   FALSE
ENST00000541489 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding retained_intron 38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 10.466539 12.848633 6.2210347 1.2312806 0.6779034 -1.0451936 0.25332917 0.27536667 0.210666667 -0.064700000 0.47977962 0.03014644 FALSE TRUE
ENST00000545991 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding retained_intron 38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 2.777054 3.031368 3.9819596 0.8756289 0.4382927 0.3923770 0.07935000 0.06666667 0.134833333 0.068166667 0.30890682 0.03014644 FALSE FALSE
ENST00000546058 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding retained_intron 38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 3.249531 3.635847 2.7121886 0.5497318 0.6603968 -0.4214871 0.08877500 0.07860000 0.092166667 0.013566667 0.94652424 0.03014644 TRUE FALSE
MSTRG.8340.4 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding   38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 2.179195 2.582942 1.8896696 1.6762561 0.9654444 -0.4488414 0.05478750 0.05076667 0.063500000 0.012733333 0.99537173 0.03014644 TRUE TRUE
MSTRG.8340.5 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding   38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 2.193869 3.544742 0.0576445 1.4956801 0.0576445 -5.7156286 0.04747500 0.07320000 0.001933333 -0.071266667 0.03014644 0.03014644 TRUE TRUE
MSTRG.8340.7 ENSG00000185163 HEK293_OSMI2_6hA HEK293_TMG_6hB DDX51 protein_coding   38.98385 46.62959 29.51992 3.992598 0.2816373 -0.6593773 2.834370 3.943649 0.6416552 0.9304511 0.5509634 -2.6010041 0.06871250 0.08300000 0.021800000 -0.061200000 0.33233330 0.03014644 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000185163 E001 0.0000000       12 132135329 132135424 96 -      
ENSG00000185163 E002 0.0000000       12 132135566 132135635 70 -      
ENSG00000185163 E003 0.0000000       12 132135949 132135983 35 -      
ENSG00000185163 E004 0.0000000       12 132136232 132136369 138 -      
ENSG00000185163 E005 0.1723744 0.0362228642 1.0000000000   12 132136370 132136593 224 - 0.000 0.085 8.670
ENSG00000185163 E006 22.2536624 0.0254731840 0.4439995171 0.661387291 12 132136594 132136691 98 - 1.376 1.304 -0.252
ENSG00000185163 E007 198.3247704 0.0010209710 0.0003997016 0.004728563 12 132136692 132137320 629 - 2.326 2.235 -0.302
ENSG00000185163 E008 97.0858628 0.0106210359 0.0019594433 0.016796677 12 132137321 132137434 114 - 2.071 1.894 -0.595
ENSG00000185163 E009 427.1195755 0.0114180803 0.0002421120 0.003132901 12 132137435 132138400 966 - 2.712 2.532 -0.599
ENSG00000185163 E010 98.0348380 0.0123651168 0.4635226287 0.675500969 12 132138401 132138579 179 - 1.985 1.948 -0.123
ENSG00000185163 E011 84.9175106 0.0039159590 0.0394128867 0.153255245 12 132138580 132138707 128 - 1.957 1.870 -0.294
ENSG00000185163 E012 162.0084310 0.0003714135 0.0000932171 0.001410346 12 132138708 132138872 165 - 2.247 2.148 -0.330
ENSG00000185163 E013 367.2100128 0.0001495762 0.0273811758 0.119434757 12 132138873 132139112 240 - 2.553 2.528 -0.086
ENSG00000185163 E014 370.2838869 0.0087707296 0.1103812705 0.299646384 12 132139113 132139298 186 - 2.474 2.559 0.282
ENSG00000185163 E015 231.6704035 1.2481353660 0.4155842194 0.640282319 12 132139635 132139769 135 - 2.149 2.391 0.808
ENSG00000185163 E016 8.2536192 0.2433069670 0.6766488749 0.820631320 12 132139770 132139854 85 - 1.037 0.882 -0.578
ENSG00000185163 E017 5.2165348 0.2675243746 0.9905303858 0.996988210 12 132139855 132139860 6 - 0.760 0.760 -0.002
ENSG00000185163 E018 216.3249719 1.2380665303 0.4387166500 0.657782538 12 132139861 132139924 64 - 2.139 2.356 0.723
ENSG00000185163 E019 4.0939701 0.1255763471 0.4982436766 0.699634744 12 132139925 132139973 49 - 0.585 0.713 0.549
ENSG00000185163 E020 259.6649015 1.3283725048 0.4839182940 0.689728461 12 132140098 132140199 102 - 2.244 2.428 0.613
ENSG00000185163 E021 1.2018237 0.0147352914 0.0352871384   12 132140422 132140422 1 - 0.000 0.434 11.663
ENSG00000185163 E022 290.1587017 0.0340162800 0.1522833445 0.365699720 12 132140423 132140539 117 - 2.342 2.461 0.399
ENSG00000185163 E023 263.5198719 0.0002663124 0.0923658314 0.268129478 12 132140620 132140735 116 - 2.351 2.409 0.195
ENSG00000185163 E024 3.3364959 0.1128473446 0.2768790614 0.516892100 12 132140736 132140821 86 - 0.730 0.532 -0.860
ENSG00000185163 E025 294.2935294 0.0046110210 0.1108988480 0.300427116 12 132140831 132141005 175 - 2.377 2.460 0.276
ENSG00000185163 E026 123.6927822 0.0105451558 0.0898876992 0.263553155 12 132141006 132141020 15 - 1.972 2.098 0.422
ENSG00000185163 E027 23.3155499 0.0836514162 0.3108700964 0.551319703 12 132141021 132141102 82 - 1.443 1.302 -0.489
ENSG00000185163 E028 57.2095576 0.0569084227 0.1606637550 0.377774713 12 132141103 132141274 172 - 1.828 1.677 -0.511
ENSG00000185163 E029 260.9228006 0.0058969173 0.1750435466 0.397225732 12 132141275 132141420 146 - 2.328 2.406 0.258
ENSG00000185163 E030 220.9242989 0.0010299600 0.2601814565 0.499155439 12 132141498 132141606 109 - 2.278 2.329 0.170
ENSG00000185163 E031 83.9147729 0.0746378151 0.0201037363 0.096236239 12 132141607 132141849 243 - 2.070 1.785 -0.958
ENSG00000185163 E032 218.3686291 0.0003535208 0.9815095075 0.992544153 12 132141850 132141956 107 - 2.298 2.315 0.057
ENSG00000185163 E033 72.1134682 0.0146379011 0.0008514826 0.008700965 12 132141957 132142118 162 - 1.974 1.746 -0.765
ENSG00000185163 E034 197.4876944 0.0003634121 0.6310009435 0.791053448 12 132142119 132142190 72 - 2.244 2.275 0.103
ENSG00000185163 E035 168.6686788 0.0028840549 0.8895759420 0.946498931 12 132142277 132142317 41 - 2.181 2.202 0.071
ENSG00000185163 E036 217.9689175 0.0062658370 0.9798478894 0.991731877 12 132142318 132142422 105 - 2.296 2.311 0.049
ENSG00000185163 E037 218.9411655 0.0003229114 0.3186201039 0.558464035 12 132142728 132142878 151 - 2.281 2.325 0.148
ENSG00000185163 E038 80.4348851 0.1513520202 0.0084369298 0.051321181 12 132142879 132143639 761 - 2.121 1.700 -1.416
ENSG00000185163 E039 116.9411770 0.0248471721 0.0188686640 0.091990774 12 132143695 132143909 215 - 1.867 2.093 0.759
ENSG00000185163 E040 58.1624840 0.1015073099 0.1635825511 0.382072090 12 132143993 132144319 327 - 1.586 1.788 0.686