ENSG00000184924

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000328379 ENSG00000184924 HEK293_OSMI2_6hA HEK293_TMG_6hB PTRHD1 protein_coding protein_coding 66.30783 75.3497 41.44076 15.89467 1.396374 -0.8623947 44.61955 55.26100 24.78931 10.606326 1.086656 -1.1562225 0.6739333 0.7402000 0.5977667 -0.1424333 0.01742141 0.0169172 FALSE TRUE
ENST00000467797 ENSG00000184924 HEK293_OSMI2_6hA HEK293_TMG_6hB PTRHD1 protein_coding processed_transcript 66.30783 75.3497 41.44076 15.89467 1.396374 -0.8623947 21.22997 19.29442 16.47012 5.612108 0.408833 -0.2282048 0.3184208 0.2480333 0.3978333 0.1498000 0.01691720 0.0169172   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000184924 E001 28.3121483 0.0648683619 1.697291e-03 0.0149991726 2 24789728 24790204 477 - 1.707 1.234 -1.630
ENSG00000184924 E002 17.6853343 0.0792227680 4.010855e-03 0.0292244967 2 24790205 24790262 58 - 1.518 1.036 -1.694
ENSG00000184924 E003 12.6031385 0.1354125401 3.216728e-02 0.1337301609 2 24790263 24790265 3 - 1.356 0.920 -1.568
ENSG00000184924 E004 15.1029928 0.0864896697 2.574812e-02 0.1145235194 2 24790266 24790274 9 - 1.400 1.013 -1.374
ENSG00000184924 E005 775.7260456 0.0005690708 8.949683e-01 0.9491949084 2 24790275 24790581 307 - 2.816 2.802 -0.047
ENSG00000184924 E006 0.8993890 1.4384701147 2.728991e-01   2 24791502 24791606 105 - 0.343 0.214 -0.923
ENSG00000184924 E007 0.1723744 6.7692096088 7.428544e-01   2 24792384 24792433 50 - 0.000 0.071 8.378
ENSG00000184924 E008 0.3150090 3.7981982223 3.933506e-01   2 24792434 24792584 151 - 0.199 0.070 -1.724
ENSG00000184924 E009 0.1723744 6.7692096088 7.428544e-01   2 24792585 24792685 101 - 0.000 0.071 8.378
ENSG00000184924 E010 0.3447487 6.9924209580 7.860762e-01   2 24792686 24792766 81 - 0.000 0.132 9.373
ENSG00000184924 E011 5.4576868 0.1800893485 1.000000e+00 1.0000000000 2 24792767 24793125 359 - 0.606 0.765 0.668
ENSG00000184924 E012 642.4691271 0.0010358966 2.465473e-05 0.0004562428 2 24793126 24793391 266 - 2.676 2.736 0.199