ENSG00000183530

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000327423 ENSG00000183530 HEK293_OSMI2_6hA HEK293_TMG_6hB PRR14L protein_coding protein_coding 11.9335 13.53427 12.71526 3.00987 0.4994848 -0.0899883 8.3520559 8.6246346 10.0103208 1.816457562 0.7067415 0.2147217 0.69134583 0.641833333 0.7855 0.14366667 1.281619e-01 3.631317e-26 FALSE TRUE
ENST00000431684 ENSG00000183530 HEK293_OSMI2_6hA HEK293_TMG_6hB PRR14L protein_coding nonsense_mediated_decay 11.9335 13.53427 12.71526 3.00987 0.4994848 -0.0899883 0.2331804 0.0117191 0.7569706 0.005983573 0.3606686 5.1421347 0.01805417 0.001033333 0.0620 0.06096667 1.808550e-02 3.631317e-26 TRUE TRUE
ENST00000432485 ENSG00000183530 HEK293_OSMI2_6hA HEK293_TMG_6hB PRR14L protein_coding nonsense_mediated_decay 11.9335 13.53427 12.71526 3.00987 0.4994848 -0.0899883 2.1270054 4.2877482 0.0000000 1.163701869 0.0000000 -8.7474371 0.19333333 0.310800000 0.0000 -0.31080000 3.631317e-26 3.631317e-26 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000183530 E001 1.1797865 0.0153787590 4.965270e-01   22 31676256 31677018 763 - 0.330 0.322 -0.054
ENSG00000183530 E002 1.3040497 0.0133666148 4.929735e-03   22 31681347 31681349 3 - 0.000 0.594 12.268
ENSG00000183530 E003 241.1701620 1.1476423263 2.054818e-01 4.364584e-01 22 31681350 31682014 665 - 1.980 2.626 2.156
ENSG00000183530 E004 111.0332905 0.6249001292 1.138922e-01 3.055969e-01 22 31682015 31682134 120 - 1.665 2.286 2.087
ENSG00000183530 E005 592.4514869 1.5541790215 3.148570e-01 5.550023e-01 22 31682135 31683628 1494 - 2.449 2.989 1.797
ENSG00000183530 E006 436.6435942 1.4989350582 3.969182e-01 6.258492e-01 22 31683629 31685041 1413 - 2.385 2.830 1.481
ENSG00000183530 E007 248.5924560 1.3362801553 4.513459e-01 6.666108e-01 22 31685042 31685803 762 - 2.195 2.559 1.215
ENSG00000183530 E008 49.1313889 0.0166192349 4.500735e-02 1.676122e-01 22 31688156 31688227 72 - 1.509 1.861 1.196
ENSG00000183530 E009 67.2866199 0.0006065136 2.871951e-01 5.279593e-01 22 31701656 31701762 107 - 1.701 1.955 0.857
ENSG00000183530 E010 62.4878323 0.0218644614 4.765426e-01 6.846746e-01 22 31703550 31703721 172 - 1.722 1.879 0.530
ENSG00000183530 E011 0.5365243 0.0256194836 7.129996e-01   22 31704444 31704654 211 - 0.109 0.239 1.363
ENSG00000183530 E012 50.1832896 0.0219740167 2.671892e-01 5.068062e-01 22 31704655 31704726 72 - 1.646 1.774 0.431
ENSG00000183530 E013 1054.0305061 0.0884960466 3.372084e-03 2.559917e-02 22 31712083 31716726 4644 - 3.043 2.991 -0.172
ENSG00000183530 E014 82.5608916 0.0149800561 9.301367e-08 3.480031e-06 22 31716727 31716985 259 - 1.984 1.824 -0.536
ENSG00000183530 E015 65.6012409 0.0166149982 2.840210e-07 9.393621e-06 22 31716986 31717181 196 - 1.888 1.719 -0.572
ENSG00000183530 E016 43.7293285 0.0201022763 3.629655e-06 8.734575e-05 22 31717182 31717291 110 - 1.719 1.535 -0.627
ENSG00000183530 E017 21.3823156 0.0230055537 7.407577e-06 1.616729e-04 22 31725538 31725543 6 - 1.440 1.184 -0.894
ENSG00000183530 E018 31.0997870 0.0067446472 2.157240e-10 1.426244e-08 22 31725544 31725610 67 - 1.602 1.339 -0.906
ENSG00000183530 E019 76.8553830 0.0296838272 1.953436e-09 1.079175e-07 22 31738387 31738911 525 - 2.008 1.668 -1.147
ENSG00000183530 E020 4.5244876 0.0483965147 1.124296e-01 3.031715e-01 22 31748124 31748247 124 - 0.765 0.675 -0.372
ENSG00000183530 E021 4.6736365 0.0058269170 6.695090e-01 8.159755e-01 22 31749391 31749474 84 - 0.698 0.812 0.459
ENSG00000183530 E022 12.3643825 0.0191495275 7.967652e-02 2.441708e-01 22 31749475 31749770 296 - 1.129 1.127 -0.006
ENSG00000183530 E023 16.0739503 0.0539496034 9.693429e-03 5.679768e-02 22 31749993 31750071 79 - 1.291 1.138 -0.541
ENSG00000183530 E024 6.9944614 0.0957118567 2.539703e-02 1.134284e-01 22 31750072 31750165 94 - 0.983 0.760 -0.858