ENSG00000182628

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330137 ENSG00000182628 HEK293_OSMI2_6hA HEK293_TMG_6hB SKA2 protein_coding protein_coding 47.662 27.43202 57.99239 5.102895 3.035723 1.079726 11.668032 4.237274 16.784417 0.8423074 0.6366108 1.9833725 0.2203958 0.15406667 0.2899000 0.13583333 0.0002567791 0.0002567791 FALSE TRUE
ENST00000578105 ENSG00000182628 HEK293_OSMI2_6hA HEK293_TMG_6hB SKA2 protein_coding protein_coding 47.662 27.43202 57.99239 5.102895 3.035723 1.079726 3.236478 2.557420 3.144564 0.7538369 0.6546187 0.2971211 0.0691125 0.08866667 0.0533000 -0.03536667 0.5246162413 0.0002567791   FALSE
ENST00000581068 ENSG00000182628 HEK293_OSMI2_6hA HEK293_TMG_6hB SKA2 protein_coding protein_coding 47.662 27.43202 57.99239 5.102895 3.035723 1.079726 19.630891 11.834937 22.801933 1.9011480 1.2672085 0.9455181 0.4143125 0.43596667 0.3939667 -0.04200000 0.7671322575 0.0002567791   FALSE
ENST00000583927 ENSG00000182628 HEK293_OSMI2_6hA HEK293_TMG_6hB SKA2 protein_coding processed_transcript 47.662 27.43202 57.99239 5.102895 3.035723 1.079726 6.343661 4.909391 9.091470 0.6188808 0.9360237 0.8876199 0.1521292 0.18476667 0.1578000 -0.02696667 0.8379661441 0.0002567791   FALSE
ENST00000583976 ENSG00000182628 HEK293_OSMI2_6hA HEK293_TMG_6hB SKA2 protein_coding nonsense_mediated_decay 47.662 27.43202 57.99239 5.102895 3.035723 1.079726 2.766537 1.953340 2.709996 0.3645663 0.5428168 0.4702942 0.0596375 0.07143333 0.0461000 -0.02533333 0.3804816537 0.0002567791   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000182628 E001 474.676939 0.0025098913 1.078677e-15 1.806549e-13 17 59109857 59111885 2029 - 2.722 2.486 -0.787
ENSG00000182628 E002 33.845065 0.0034694746 2.863287e-01 5.270042e-01 17 59111886 59111887 2 - 1.554 1.462 -0.315
ENSG00000182628 E003 32.407536 0.0071562605 3.473669e-01 5.848471e-01 17 59111888 59111889 2 - 1.534 1.446 -0.302
ENSG00000182628 E004 42.345934 0.0015077600 6.613978e-01 8.108125e-01 17 59111890 59111942 53 - 1.632 1.585 -0.159
ENSG00000182628 E005 30.842167 0.0014345014 1.263405e-01 3.260024e-01 17 59111943 59111955 13 - 1.524 1.397 -0.435
ENSG00000182628 E006 91.219575 0.0005151050 3.840871e-01 6.156495e-01 17 59111956 59112046 91 - 1.964 1.906 -0.193
ENSG00000182628 E007 101.137183 0.0005156946 6.353765e-01 7.938223e-01 17 59112047 59112090 44 - 1.990 1.984 -0.020
ENSG00000182628 E008 300.157733 0.0002355170 6.401946e-03 4.169110e-02 17 59112091 59112261 171 - 2.447 2.480 0.108
ENSG00000182628 E009 291.360118 0.0002397472 7.177314e-04 7.592805e-03 17 59112262 59112345 84 - 2.431 2.479 0.157
ENSG00000182628 E010 1.493698 0.0126555126 6.315578e-01 7.914010e-01 17 59119299 59119318 20 - 0.376 0.448 0.390
ENSG00000182628 E011 135.927844 0.0011582391 2.876555e-01 5.283880e-01 17 59119319 59119386 68 - 2.115 2.127 0.042
ENSG00000182628 E012 148.471595 0.0008021964 7.324531e-02 2.313194e-01 17 59119387 59119495 109 - 2.146 2.180 0.113
ENSG00000182628 E013 1.042211 0.0152252534 5.757979e-01   17 59124307 59124428 122 - 0.283 0.372 0.561
ENSG00000182628 E014 201.305358 0.0002265330 8.152400e-05 1.261472e-03 17 59131281 59131367 87 - 2.260 2.336 0.253
ENSG00000182628 E015 0.000000       17 59151769 59151801 33 -      
ENSG00000182628 E016 24.526222 0.0017627217 5.001922e-02 1.797223e-01 17 59154956 59155130 175 - 1.350 1.469 0.413
ENSG00000182628 E017 168.345598 0.0003315331 1.073505e-06 3.024820e-05 17 59155131 59155260 130 - 2.169 2.282 0.375