ENSG00000182544

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329548 ENSG00000182544 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD5 protein_coding protein_coding 40.58526 52.04709 22.3447 3.328337 0.8134997 -1.219517 31.270342 46.811395 13.732127 3.6770237 0.6946809 -1.768563 0.7629875 0.89803333 0.6181000 -0.2799333 0.000562657 0.000562657 FALSE TRUE
ENST00000552097 ENSG00000182544 HEK293_OSMI2_6hA HEK293_TMG_6hB MFSD5 protein_coding processed_transcript 40.58526 52.04709 22.3447 3.328337 0.8134997 -1.219517 6.633802 1.798328 6.565594 0.9119444 1.2155530 1.862465 0.1670000 0.03626667 0.2906333 0.2543667 0.334811252 0.000562657 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000182544 E001 1.035009 2.0711857280 0.2289330231   12 53251065 53251250 186 + 0.545 0.159 -2.498
ENSG00000182544 E002 15.361376 0.0318599784 0.0339626382 0.138757386 12 53251251 53251585 335 + 1.323 1.052 -0.962
ENSG00000182544 E003 8.410620 0.0133510969 1.0000000000 1.000000000 12 53251586 53251624 39 + 0.890 0.873 -0.066
ENSG00000182544 E004 1.791331 0.8402909014 0.1502351766 0.362659992 12 53251625 53251652 28 + 0.631 0.304 -1.692
ENSG00000182544 E005 2.530402 0.0100487836 0.2008138378 0.430732417 12 53251653 53251704 52 + 0.642 0.430 -1.003
ENSG00000182544 E006 17.549260 0.0071851558 0.0355782210 0.143141441 12 53251705 53252035 331 + 1.328 1.114 -0.755
ENSG00000182544 E007 1.648986 1.5038740025 0.7691863730 0.878162064 12 53252095 53252104 10 + 0.226 0.380 1.035
ENSG00000182544 E008 116.199220 0.0041074617 0.0003928662 0.004661663 12 53252105 53252268 164 + 1.799 1.993 0.653
ENSG00000182544 E009 3.459531 0.0079357901 0.2995186650 0.539946905 12 53252449 53252483 35 + 0.704 0.538 -0.727
ENSG00000182544 E010 116.564602 0.0004957422 0.0019424272 0.016685322 12 53252818 53252866 49 + 1.858 1.986 0.431
ENSG00000182544 E011 1379.324508 0.0006136721 0.0041742089 0.030131910 12 53252867 53254406 1540 + 3.048 3.027 -0.070