ENSG00000182185

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000471583 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding protein_coding 5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 1.32099724 0.6438960 1.4742581 0.09230721 0.13021169 1.1826088 0.29073333 0.29286667 0.31070000 0.01783333 0.96730286 0.00339764 FALSE TRUE
ENST00000479335 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding nonsense_mediated_decay 5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 0.03530173 0.1123420 0.0000000 0.02836526 0.00000000 -3.6128483 0.00883750 0.05253333 0.00000000 -0.05253333 0.00339764 0.00339764 FALSE TRUE
ENST00000487270 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding protein_coding 5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 0.55423285 0.2055049 0.5011318 0.01042810 0.12463938 1.2459747 0.10372917 0.09470000 0.10593333 0.01123333 0.99007278 0.00339764 FALSE TRUE
ENST00000488612 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding protein_coding 5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 0.26127141 0.2552844 0.0713168 0.04923307 0.04089976 -1.7059146 0.05477917 0.11860000 0.01490000 -0.10370000 0.01874268 0.00339764 FALSE TRUE
ENST00000554575 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding protein_coding 5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 0.36865975 0.1729652 0.3398461 0.17296525 0.33984611 0.9351508 0.06442500 0.07853333 0.06440000 -0.01413333 0.97465410 0.00339764   FALSE
MSTRG.9774.4 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding   5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 0.79960829 0.2408459 0.4639452 0.08722004 0.23263568 0.9179190 0.13782917 0.10816667 0.09446667 -0.01370000 0.94193032 0.00339764 FALSE TRUE
MSTRG.9774.5 ENSG00000182185 HEK293_OSMI2_6hA HEK293_TMG_6hB RAD51B protein_coding   5.377892 2.179403 4.77329 0.09377247 0.2531021 1.127466 1.16834162 0.2856767 1.5294366 0.12819096 0.09220689 2.3803099 0.19074583 0.12676667 0.32360000 0.19683333 0.20872695 0.00339764 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000182185 E001 0.4458772 0.023220523 0.2683234348   14 67819779 67819797 19 + 0.092 0.275 1.912
ENSG00000182185 E002 0.7912460 0.018783037 0.2580446737   14 67819798 67819800 3 + 0.167 0.367 1.500
ENSG00000182185 E003 0.7912460 0.018783037 0.2580446737   14 67819801 67819807 7 + 0.167 0.367 1.500
ENSG00000182185 E004 0.9338807 0.093972231 0.4567296461   14 67819808 67819811 4 + 0.230 0.368 0.930
ENSG00000182185 E005 5.1541457 0.006442200 0.7692294916 0.878181721 14 67819812 67819828 17 + 0.774 0.735 -0.154
ENSG00000182185 E006 8.6651344 0.036209507 0.9678237690 0.985774616 14 67819829 67819853 25 + 0.962 0.959 -0.011
ENSG00000182185 E007 0.3503582 0.027013616 0.6479001592   14 67822165 67822330 166 + 0.092 0.159 0.912
ENSG00000182185 E008 0.1426347 0.033674569 0.8908954662   14 67823520 67823541 22 + 0.092 0.000 -10.237
ENSG00000182185 E009 15.9115748 0.002566474 0.6957069800 0.832713427 14 67823542 67823627 86 + 1.192 1.229 0.131
ENSG00000182185 E010 22.8159391 0.002853455 0.4717261296 0.681332317 14 67825464 67825577 114 + 1.341 1.396 0.193
ENSG00000182185 E011 25.4756884 0.002922856 0.4907240477 0.694486486 14 67835080 67835196 117 + 1.389 1.440 0.177
ENSG00000182185 E012 0.0000000       14 67843433 67843500 68 +      
ENSG00000182185 E013 0.0000000       14 67857892 67858057 166 +      
ENSG00000182185 E014 0.6368449 0.019100555 0.0074609835   14 67864788 67864885 98 + 0.000 0.442 14.027
ENSG00000182185 E015 15.2287296 0.003051835 0.9859219886 0.994792367 14 67865003 67865031 29 + 1.192 1.194 0.005
ENSG00000182185 E016 23.2588061 0.007265538 0.3284379164 0.567455259 14 67865032 67865139 108 + 1.389 1.313 -0.265
ENSG00000182185 E017 0.0000000       14 67885733 67885868 136 +      
ENSG00000182185 E018 22.7324799 0.005987567 0.4616162106 0.674290447 14 67885869 67885988 120 + 1.368 1.313 -0.193
ENSG00000182185 E019 0.0000000       14 67886598 67886629 32 +      
ENSG00000182185 E020 0.0000000       14 67886756 67886857 102 +      
ENSG00000182185 E021 13.1085773 0.002980695 0.9842789171 0.994009737 14 67887021 67887066 46 + 1.122 1.127 0.020
ENSG00000182185 E022 13.9938671 0.002756328 0.9385368612 0.971577519 14 67887067 67887101 35 + 1.144 1.155 0.039
ENSG00000182185 E023 13.8334493 0.002372979 0.8245154523 0.910615400 14 67887102 67887129 28 + 1.144 1.168 0.085
ENSG00000182185 E024 15.1970304 0.002403690 0.9752812797 0.989481381 14 67887130 67887204 75 + 1.193 1.193 0.002
ENSG00000182185 E025 0.0000000       14 67887205 67887550 346 +      
ENSG00000182185 E026 0.1308682 0.033901760 0.2642920024   14 67908305 67908853 549 + 0.000 0.159 12.025
ENSG00000182185 E027 0.0000000       14 68000279 68000313 35 +      
ENSG00000182185 E028 0.0000000       14 68069569 68069647 79 +      
ENSG00000182185 E029 18.2228510 0.002196400 0.6286454900 0.789650461 14 68291884 68291980 97 + 1.276 1.240 -0.126
ENSG00000182185 E030 0.1779838 0.033006925 0.8903655985   14 68387137 68387186 50 + 0.092 0.000 -10.240
ENSG00000182185 E031 18.4053626 0.002507268 0.9164581391 0.960421852 14 68411424 68411509 86 + 1.259 1.272 0.045
ENSG00000182185 E032 13.6487427 0.005523041 0.0830270245 0.250812003 14 68411510 68411527 18 + 1.200 1.030 -0.609
ENSG00000182185 E033 20.2766172 0.021534971 0.4635032481 0.675478521 14 68468172 68468250 79 + 1.330 1.260 -0.246
ENSG00000182185 E034 0.0000000       14 68468555 68468599 45 +      
ENSG00000182185 E035 2.1847408 0.025910405 0.7688082862 0.877918840 14 68470523 68470717 195 + 0.494 0.444 -0.254
ENSG00000182185 E036 0.1779838 0.033006925 0.8903655985   14 68472033 68472119 87 + 0.092 0.000 -10.240
ENSG00000182185 E037 11.3242566 0.003047425 0.2596212954 0.498587263 14 68477648 68478101 454 + 1.027 1.141 0.414
ENSG00000182185 E038 7.4346658 0.004384055 0.2468791549 0.484556409 14 68497124 68497881 758 + 0.943 0.801 -0.544
ENSG00000182185 E039 0.2735028 0.027968687 0.6484139804   14 68540200 68540249 50 + 0.092 0.159 0.911
ENSG00000182185 E040 0.3453689 0.027040388 0.6468108144   14 68545562 68545624 63 + 0.092 0.159 0.917
ENSG00000182185 E041 0.1779838 0.033006925 0.8903655985   14 68563550 68563618 69 + 0.092 0.000 -10.240
ENSG00000182185 E042 13.2987687 0.032247399 0.8213350546 0.908766709 14 68594485 68595996 1512 + 1.142 1.112 -0.105
ENSG00000182185 E043 2.0605143 0.287742283 0.1333415506 0.337016945 14 68611006 68611565 560 + 0.285 0.714 2.169
ENSG00000182185 E044 0.1723744 0.033161816 0.2629488006   14 68650781 68650856 76 + 0.000 0.159 12.030
ENSG00000182185 E045 4.3957463 0.006506998 0.0650639014 0.214030933 14 68682937 68683171 235 + 0.791 0.507 -1.224
ENSG00000182185 E046 2.2449449 0.010231984 0.0001117727 0.001639247 14 68729808 68730218 411 + 0.167 0.800 3.497