ENSG00000180448

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000313093 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding protein_coding 1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.27377603 0.62636710 0.00000000 0.13310065 0.00000000 -5.9917874 0.19106250 0.36610000 0.0000000 -0.36610000 1.100378e-05 5.669303e-10 FALSE TRUE
ENST00000543365 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding protein_coding 1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.03948903 0.10685121 0.04744830 0.10685121 0.04744830 -1.0243365 0.06418333 0.04833333 0.1185000 0.07016667 1.000000e+00 5.669303e-10 FALSE TRUE
ENST00000586378 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding retained_intron 1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.21496850 0.64967176 0.00000000 0.12937323 0.00000000 -6.0436764 0.14969167 0.36906667 0.0000000 -0.36906667 5.669303e-10 5.669303e-10 FALSE TRUE
ENST00000586866 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding protein_coding 1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.02708124 0.00000000 0.10312883 0.00000000 0.05371049 3.4998948 0.05985000 0.00000000 0.1723667 0.17236667 5.883426e-01 5.669303e-10 FALSE TRUE
ENST00000590577 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding protein_coding 1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.15298839 0.20527843 0.02747714 0.06890204 0.01624357 -2.5221209 0.17054583 0.14303333 0.0230000 -0.12003333 2.834450e-01 5.669303e-10 FALSE TRUE
ENST00000591169 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding nonsense_mediated_decay 1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.69262568 0.00000000 5.10881926 0.00000000 4.99334635 8.9996673 0.09518333 0.00000000 0.5182000 0.51820000 1.973472e-01 5.669303e-10 FALSE TRUE
MSTRG.16036.12 ENSG00000180448 HEK293_OSMI2_6hA HEK293_TMG_6hB ARHGAP45 protein_coding   1.670134 1.742788 5.375884 0.3099543 5.048585 1.619532 0.07434358 0.07619979 0.04196576 0.07619979 0.04196576 -0.7301229 0.09237917 0.03446667 0.1648667 0.13040000 1.000000e+00 5.669303e-10 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000180448 E001 0.6071052 0.070949372 1.000000e+00   19 1065923 1066163 241 + 0.252 0.144 -0.995
ENSG00000180448 E002 0.0000000       19 1066509 1066641 133 +      
ENSG00000180448 E003 1.2865538 0.163836468 9.555072e-02   19 1067167 1067316 150 + 0.000 0.292 27.892
ENSG00000180448 E004 1.1141795 0.217085939 1.602312e-01   19 1067317 1067318 2 + 0.000 0.261 27.678
ENSG00000180448 E005 1.3759158 0.025522190 3.922625e-02   19 1067319 1067326 8 + 0.000 0.314 28.039
ENSG00000180448 E006 2.5298837 0.011516449 1.843315e-03 1.601213e-02 19 1067327 1067495 169 + 0.000 0.475 28.944
ENSG00000180448 E007 0.3503582 0.026335917 5.638495e-01   19 1067498 1067674 177 + 0.253 0.054 -2.578
ENSG00000180448 E008 0.0000000       19 1068333 1068413 81 +      
ENSG00000180448 E009 4.3059188 0.062483201 1.685984e-02 8.493547e-02 19 1068414 1068744 331 + 0.412 0.612 0.967
ENSG00000180448 E010 0.0000000       19 1071221 1071358 138 +      
ENSG00000180448 E011 0.0000000       19 1071513 1071766 254 +      
ENSG00000180448 E012 2.8399761 0.236273997 5.666906e-01 7.477746e-01 19 1073149 1073223 75 + 0.525 0.460 -0.319
ENSG00000180448 E013 3.7858606 0.021752274 3.374212e-01 5.757825e-01 19 1073224 1073292 69 + 0.694 0.529 -0.728
ENSG00000180448 E014 4.6970161 0.252272814 7.199860e-01 8.481880e-01 19 1073506 1073582 77 + 0.861 0.577 -1.170
ENSG00000180448 E015 2.5489952 0.374403769 8.232566e-01 9.098053e-01 19 1073583 1073590 8 + 0.613 0.410 -0.981
ENSG00000180448 E016 0.0000000       19 1073591 1073673 83 +      
ENSG00000180448 E017 3.8293920 0.119534389 4.082127e-01 6.346285e-01 19 1073674 1073746 73 + 0.619 0.548 -0.317
ENSG00000180448 E018 3.6078768 0.066279994 3.260238e-01 5.652453e-01 19 1073948 1074014 67 + 0.618 0.529 -0.401
ENSG00000180448 E019 3.7789659 0.255393062 5.112914e-01 7.089528e-01 19 1074104 1074241 138 + 0.614 0.555 -0.264
ENSG00000180448 E020 3.1837471 0.258893412 2.343370e-01 4.700774e-01 19 1074343 1074407 65 + 0.407 0.532 0.632
ENSG00000180448 E021 3.1831269 0.016755348 3.377672e-03 2.562687e-02 19 1074614 1074724 111 + 0.252 0.559 1.735
ENSG00000180448 E022 3.3840236 0.179814399 2.831618e-01 5.235962e-01 19 1074799 1074879 81 + 0.525 0.536 0.049
ENSG00000180448 E023 0.4873834 0.821901197 7.163850e-01   19 1077487 1077856 370 + 0.248 0.104 -1.505
ENSG00000180448 E024 7.3351190 0.291444586 6.679851e-01 8.149147e-01 19 1077857 1078045 189 + 0.860 0.776 -0.329
ENSG00000180448 E025 4.1891788 0.058590744 3.040108e-01 5.445822e-01 19 1079703 1079757 55 + 0.695 0.575 -0.517
ENSG00000180448 E026 5.5541359 0.086631219 2.917689e-01 5.324401e-01 19 1079758 1079840 83 + 0.760 0.672 -0.362
ENSG00000180448 E027 6.8694046 0.006113754 6.422299e-02 2.121895e-01 19 1079928 1080118 191 + 0.864 0.739 -0.492
ENSG00000180448 E028 6.3087973 0.005382027 4.694804e-03 3.297681e-02 19 1080255 1080379 125 + 0.694 0.749 0.226
ENSG00000180448 E029 0.0000000       19 1080380 1080463 84 +      
ENSG00000180448 E030 6.1356151 0.005355901 2.775725e-04 3.504438e-03 19 1080464 1080547 84 + 0.527 0.759 1.003
ENSG00000180448 E031 5.6349876 0.005739947 9.361073e-06 1.980719e-04 19 1080682 1080786 105 + 0.252 0.749 2.547
ENSG00000180448 E032 5.4827318 0.006352092 1.306977e-06 3.583963e-05 19 1080892 1081064 173 + 0.000 0.729 30.081
ENSG00000180448 E033 0.0000000       19 1081415 1081549 135 +      
ENSG00000180448 E034 5.4902466 0.041076087 1.916543e-03 1.651728e-02 19 1081550 1081738 189 + 0.411 0.701 1.348
ENSG00000180448 E035 5.2923318 0.005758650 1.863281e-03 1.614676e-02 19 1081824 1081961 138 + 0.527 0.695 0.742
ENSG00000180448 E036 0.5061644 0.860863295 7.034371e-01   19 1082521 1082657 137 + 0.253 0.104 -1.544
ENSG00000180448 E037 0.4341107 0.026852359 4.170007e-01   19 1082784 1082839 56 + 0.000 0.145 26.615
ENSG00000180448 E038 4.0524458 0.007166976 2.276048e-05 4.267697e-04 19 1082840 1082840 1 + 0.000 0.646 29.731
ENSG00000180448 E039 6.8817707 0.111414934 1.875610e-02 9.156609e-02 19 1082841 1082938 98 + 0.526 0.797 1.161
ENSG00000180448 E040 7.0945537 0.305389007 2.099506e-01 4.417872e-01 19 1082939 1083066 128 + 0.614 0.800 0.769
ENSG00000180448 E041 0.6011358 0.074028311 4.326366e-01   19 1083067 1083142 76 + 0.412 0.101 -2.596
ENSG00000180448 E042 7.0952914 0.058807534 2.451687e-02 1.107321e-01 19 1083143 1083275 133 + 0.696 0.791 0.387
ENSG00000180448 E043 8.5058864 0.106795568 1.272060e-01 3.274100e-01 19 1083276 1083353 78 + 0.815 0.845 0.120
ENSG00000180448 E044 9.9846773 0.107351748 4.235752e-01 6.462903e-01 19 1084238 1084346 109 + 1.020 0.878 -0.533
ENSG00000180448 E045 88.6925963 0.688024376 4.212251e-01 6.445581e-01 19 1085660 1086628 969 + 2.494 1.339 -3.900