ENSG00000179965

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000319296 ENSG00000179965 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF771 protein_coding protein_coding 13.54784 18.17119 6.989113 4.043166 0.07119796 -1.377203 9.7622947 13.2821254 3.9989257 3.1396453 0.12983079 -1.7292842 0.69407083 0.72666667 0.57210000 -0.15456667 0.02446778 0.02446778 FALSE TRUE
ENST00000566625 ENSG00000179965 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF771 protein_coding protein_coding 13.54784 18.17119 6.989113 4.043166 0.07119796 -1.377203 0.7581316 0.6068023 1.0970825 0.1044168 0.27232455 0.8438826 0.07664167 0.03873333 0.15700000 0.11826667 0.07555932 0.02446778   FALSE
MSTRG.12449.1 ENSG00000179965 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF771 protein_coding   13.54784 18.17119 6.989113 4.043166 0.07119796 -1.377203 0.7344543 1.1266321 0.3530675 0.2372089 0.07150644 -1.6464558 0.05483750 0.06323333 0.05043333 -0.01280000 0.82164918 0.02446778   FALSE
MSTRG.12449.2 ENSG00000179965 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF771 protein_coding   13.54784 18.17119 6.989113 4.043166 0.07119796 -1.377203 1.6470242 1.8558558 1.4433204 0.3817658 0.15658541 -0.3604847 0.13003333 0.10783333 0.20690000 0.09906667 0.22403368 0.02446778 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000179965 E001 0.3503582 0.0458458926 0.3741293133   16 30403115 30403240 126 + 0.200 0.071 -1.716
ENSG00000179965 E002 12.6294130 0.0149014338 0.6463471979 0.801092607 16 30406947 30407349 403 + 1.104 1.069 -0.125
ENSG00000179965 E003 16.5891281 0.0063702282 0.8889757897 0.946155274 16 30407350 30407413 64 + 1.160 1.199 0.141
ENSG00000179965 E004 39.2703895 0.0261890852 0.4425867655 0.660452003 16 30407414 30407605 192 + 1.455 1.560 0.358
ENSG00000179965 E005 106.5451407 0.0005321570 0.7189084874 0.847522702 16 30407606 30407628 23 + 1.936 1.975 0.130
ENSG00000179965 E006 227.4145331 0.0003147277 0.2508538727 0.488910841 16 30407629 30407664 36 + 2.298 2.291 -0.024
ENSG00000179965 E007 1.2427327 0.0139430539 0.0938367975   16 30408031 30408031 1 + 0.523 0.233 -1.715
ENSG00000179965 E008 32.8922017 0.0012697078 0.0786337881 0.242039908 16 30408032 30408044 13 + 1.536 1.439 -0.332
ENSG00000179965 E009 319.4660297 0.0002689760 0.0037967612 0.028000928 16 30408045 30408194 150 + 2.468 2.430 -0.127
ENSG00000179965 E010 215.5424908 0.0262295819 0.2812673994 0.521544724 16 30413587 30417554 3968 + 2.319 2.241 -0.258
ENSG00000179965 E011 595.7890390 0.0090623254 0.0002485024 0.003203145 16 30417555 30420305 2751 + 2.580 2.732 0.506
ENSG00000179965 E012 65.9811404 0.1719080558 0.3425197419 0.580238310 16 30428880 30431108 2229 + 1.940 1.676 -0.894