ENSG00000179454

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355081 ENSG00000179454 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL28 protein_coding protein_coding 3.222087 1.424356 5.287945 0.2039206 0.4347562 1.88503 0.5711013 0.19483157 1.02611373 0.10881875 0.05696803 2.3386724 0.15090417 0.16633333 0.19496667 0.02863333 0.864293306 0.002218746 FALSE TRUE
ENST00000396128 ENSG00000179454 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL28 protein_coding protein_coding 3.222087 1.424356 5.287945 0.2039206 0.4347562 1.88503 1.4623836 0.33755957 3.00106697 0.07040057 0.08188418 3.1149426 0.39148750 0.24866667 0.57340000 0.32473333 0.031817281 0.002218746 FALSE TRUE
ENST00000556500 ENSG00000179454 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL28 protein_coding protein_coding 3.222087 1.424356 5.287945 0.2039206 0.4347562 1.88503 0.1998928 0.11343700 0.32977798 0.05698267 0.04454176 1.4608175 0.05612917 0.07466667 0.06266667 -0.01200000 0.935653231 0.002218746 FALSE FALSE
ENST00000579157 ENSG00000179454 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL28 protein_coding protein_coding 3.222087 1.424356 5.287945 0.2039206 0.4347562 1.88503 0.1044369 0.05166415 0.05955883 0.05166415 0.03068648 0.1738017 0.05347083 0.02930000 0.01113333 -0.01816667 0.933434893 0.002218746 FALSE FALSE
MSTRG.9456.5 ENSG00000179454 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL28 protein_coding   3.222087 1.424356 5.287945 0.2039206 0.4347562 1.88503 0.2122123 0.47070218 0.05578831 0.12306185 0.05578831 -2.8692402 0.10555833 0.31953333 0.01146667 -0.30806667 0.002218746 0.002218746   FALSE
MSTRG.9456.6 ENSG00000179454 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL28 protein_coding   3.222087 1.424356 5.287945 0.2039206 0.4347562 1.88503 0.4559297 0.25616136 0.71904111 0.12840442 0.24328073 1.4536990 0.18595833 0.16146667 0.13063333 -0.03083333 0.975970739 0.002218746 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000179454 E001 162.7732062 0.0007837281 5.974849e-09 2.935142e-07 14 44924324 44928812 4489 - 2.106 1.916 -0.637
ENSG00000179454 E002 10.5118552 0.0036489636 1.374315e-02 7.333089e-02 14 44928813 44928938 126 - 0.978 0.601 -1.508
ENSG00000179454 E003 19.1154574 0.0021145131 5.348345e-03 3.643435e-02 14 44928939 44929191 253 - 1.209 0.888 -1.173
ENSG00000179454 E004 19.4391617 0.0063139520 2.166997e-04 2.859854e-03 14 44931333 44931541 209 - 1.228 0.739 -1.826
ENSG00000179454 E005 34.6715700 0.0014098051 1.057033e-03 1.034631e-02 14 44934115 44934558 444 - 1.456 1.182 -0.958
ENSG00000179454 E006 4.1256532 0.0565592016 3.704076e-05 6.459616e-04 14 44937703 44937812 110 - 0.403 1.104 2.934
ENSG00000179454 E007 18.3618183 0.0273443779 8.958476e-02 2.630511e-01 14 44945030 44945115 86 - 1.123 1.336 0.753
ENSG00000179454 E008 26.3553374 0.0073874360 2.672847e-02 1.174657e-01 14 44945116 44945232 117 - 1.269 1.477 0.722
ENSG00000179454 E009 33.8984918 0.0028970111 9.747365e-03 5.703303e-02 14 44945233 44945440 208 - 1.370 1.576 0.708
ENSG00000179454 E010 13.7186589 0.1083735534 7.365879e-01 8.583240e-01 14 44945441 44945495 55 - 1.020 1.125 0.380
ENSG00000179454 E011 11.2667011 0.0745875730 2.627419e-02 1.161298e-01 14 44945496 44945525 30 - 0.873 1.249 1.372
ENSG00000179454 E012 30.0913462 0.0208750164 7.812538e-02 2.410816e-01 14 44945526 44945676 151 - 1.326 1.512 0.645
ENSG00000179454 E013 31.7756349 0.0017373009 5.176744e-01 7.133944e-01 14 44945677 44945791 115 - 1.377 1.446 0.236
ENSG00000179454 E014 18.1657940 0.0020086345 8.454944e-01 9.224055e-01 14 44945792 44945808 17 - 1.162 1.160 -0.007
ENSG00000179454 E015 34.6328450 0.0016828496 1.784326e-02 8.851165e-02 14 44945809 44945928 120 - 1.390 1.575 0.636
ENSG00000179454 E016 0.4702677 0.0217681645 4.430535e-01   14 44953623 44953709 87 - 0.107 0.242 1.421
ENSG00000179454 E017 0.1614157 0.0333070922 1.000000e+00   14 44959341 44959514 174 - 0.057 0.001 -5.956
ENSG00000179454 E018 0.6544085 0.0190815585 6.393378e-01   14 44960887 44961132 246 - 0.152 0.243 0.840
ENSG00000179454 E019 3.1555186 0.0073147583 4.532313e-02 1.683954e-01 14 44961597 44961845 249 - 0.449 0.794 1.531
ENSG00000179454 E020 22.7331648 0.0147279845 3.713967e-02 1.472881e-01 14 44961846 44962190 345 - 1.203 1.421 0.761
ENSG00000179454 E021 0.0000000       14 44963851 44964092 242 -      
ENSG00000179454 E022 0.0000000       14 44978198 44978298 101 -      
ENSG00000179454 E023 0.0000000       14 45042116 45042322 207 -