ENSG00000177885

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316804 ENSG00000177885 HEK293_OSMI2_6hA HEK293_TMG_6hB GRB2 protein_coding protein_coding 77.69708 83.21479 67.9568 10.14127 5.383607 -0.2921831 60.990221 74.90023683 41.858261 8.37040247 1.981115 -0.8393059 0.7854958 0.9023667 0.62506667 -0.27730000 1.912259e-02 2.890474e-26 FALSE TRUE
ENST00000392564 ENSG00000177885 HEK293_OSMI2_6hA HEK293_TMG_6hB GRB2 protein_coding protein_coding 77.69708 83.21479 67.9568 10.14127 5.383607 -0.2921831 9.934295 6.01359301 12.089707 3.30803055 2.900562 1.0062759 0.1208375 0.0695000 0.17496667 0.10546667 7.604973e-01 2.890474e-26 FALSE TRUE
ENST00000462266 ENSG00000177885 HEK293_OSMI2_6hA HEK293_TMG_6hB GRB2 protein_coding processed_transcript 77.69708 83.21479 67.9568 10.14127 5.383607 -0.2921831 1.928143 0.07859057 4.240716 0.07859057 2.241925 5.5844088 0.0264000 0.0010000 0.05763333 0.05663333 4.556608e-01 2.890474e-26 FALSE TRUE
ENST00000582582 ENSG00000177885 HEK293_OSMI2_6hA HEK293_TMG_6hB GRB2 protein_coding protein_coding 77.69708 83.21479 67.9568 10.14127 5.383607 -0.2921831 1.833081 0.00000000 8.036934 0.00000000 1.677758 9.6522954 0.0272125 0.0000000 0.11563333 0.11563333 2.890474e-26 2.890474e-26 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000177885 E001 0.9513064 0.2712144140 9.120742e-01   17 75289729 75289849 121 - 0.267 0.295 0.191
ENSG00000177885 E002 38.6915318 0.0219795777 3.757805e-01 6.088367e-01 17 75318076 75318120 45 - 1.504 1.613 0.373
ENSG00000177885 E003 122.7137644 0.0128708719 1.413374e-01 3.494756e-01 17 75318121 75318178 58 - 1.992 2.114 0.408
ENSG00000177885 E004 342.1254467 0.0043715596 3.513489e-04 4.252841e-03 17 75318179 75318297 119 - 2.415 2.567 0.508
ENSG00000177885 E005 1240.4211586 0.0012344878 1.193956e-04 1.731374e-03 17 75318298 75318754 457 - 3.022 3.105 0.277
ENSG00000177885 E006 423.0127870 0.0009683116 5.968748e-01 7.682280e-01 17 75318755 75318796 42 - 2.595 2.617 0.073
ENSG00000177885 E007 1578.5252224 0.0011006191 5.600250e-06 1.272309e-04 17 75318797 75319478 682 - 3.123 3.212 0.296
ENSG00000177885 E008 707.2615620 0.0001125207 7.186495e-03 4.551494e-02 17 75319479 75319830 352 - 2.800 2.846 0.152
ENSG00000177885 E009 542.1069314 0.0006669644 7.804248e-01 8.850238e-01 17 75319831 75320010 180 - 2.705 2.720 0.049
ENSG00000177885 E010 838.0232788 0.0018351024 3.957530e-01 6.250275e-01 17 75320011 75320340 330 - 2.911 2.903 -0.027
ENSG00000177885 E011 217.1756088 0.0043642918 5.382820e-02 1.886859e-01 17 75320341 75320344 4 - 2.363 2.295 -0.227
ENSG00000177885 E012 812.8180694 0.0008111747 1.860264e-03 1.612615e-02 17 75320345 75320550 206 - 2.920 2.874 -0.152
ENSG00000177885 E013 345.6171965 0.0001947009 5.404882e-04 6.041423e-03 17 75320551 75320553 3 - 2.558 2.496 -0.208
ENSG00000177885 E014 694.2834590 0.0001522661 2.065086e-06 5.368118e-05 17 75321659 75321794 136 - 2.858 2.797 -0.202
ENSG00000177885 E015 430.2413747 0.0007900468 7.187373e-04 7.602499e-03 17 75321795 75321827 33 - 2.653 2.589 -0.212
ENSG00000177885 E016 703.7785916 0.0018687181 7.783592e-04 8.100956e-03 17 75325898 75326020 123 - 2.872 2.803 -0.228
ENSG00000177885 E017 0.5885118 0.0343910614 6.084021e-01   17 75326239 75326338 100 - 0.263 0.175 -0.745
ENSG00000177885 E018 649.6141434 0.0009846487 6.802721e-06 1.502678e-04 17 75332700 75332797 98 - 2.842 2.763 -0.264
ENSG00000177885 E019 0.3503582 0.0306671245 6.981994e-01   17 75387490 75387794 305 - 0.152 0.097 -0.747
ENSG00000177885 E020 1.9835839 0.0259472161 7.942561e-01 8.928853e-01 17 75393404 75393550 147 - 0.427 0.477 0.258
ENSG00000177885 E021 709.6345849 0.0001371191 2.720559e-03 2.168670e-02 17 75393551 75393765 215 - 2.852 2.817 -0.117
ENSG00000177885 E022 4.4603353 0.0072098768 5.175807e-01 7.133372e-01 17 75393766 75393864 99 - 0.638 0.740 0.426
ENSG00000177885 E023 0.0000000       17 75393919 75394033 115 -      
ENSG00000177885 E024 0.8507255 0.0238765657 6.454106e-02   17 75404841 75405488 648 - 0.001 0.352 9.896
ENSG00000177885 E025 220.3602879 0.0023081872 1.750801e-06 4.639199e-05 17 75405489 75405709 221 - 2.208 2.384 0.587