ENSG00000177034

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000512528 ENSG00000177034 HEK293_OSMI2_6hA HEK293_TMG_6hB MTX3 protein_coding protein_coding 6.0246 2.355125 11.72012 0.2656469 0.2443732 2.31023 3.7481028 0.8061778 8.373097 0.1544275 0.1342201 3.36052795 0.51028750 0.33703333 0.7150000 0.3779667 7.387848e-07 1.245092e-14 FALSE TRUE
ENST00000617335 ENSG00000177034 HEK293_OSMI2_6hA HEK293_TMG_6hB MTX3 protein_coding protein_coding 6.0246 2.355125 11.72012 0.2656469 0.2443732 2.31023 1.4929485 1.4571180 1.475649 0.1202311 0.1502225 0.01810853 0.40491250 0.62353333 0.1255667 -0.4979667 1.245092e-14 1.245092e-14 FALSE TRUE
MSTRG.26477.1 ENSG00000177034 HEK293_OSMI2_6hA HEK293_TMG_6hB MTX3 protein_coding   6.0246 2.355125 11.72012 0.2656469 0.2443732 2.31023 0.6486691 0.0324331 1.844243 0.0324331 0.1464442 5.44949681 0.06761667 0.01686667 0.1570333 0.1401667 1.532796e-03 1.245092e-14 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000177034 E001 0.3393995 0.08535989 0.8149414651   5 79975582 79975685 104 - 0.085 0.000 -7.954
ENSG00000177034 E002 0.8628224 0.02978096 0.7824539482   5 79976716 79976730 15 - 0.187 0.293 0.837
ENSG00000177034 E003 415.9406808 1.51208456 0.2330804492 0.468708361 5 79976731 79979760 3030 - 2.271 2.924 2.174
ENSG00000177034 E004 8.1968489 0.05095321 0.0866768747 0.257667385 5 79979761 79979776 16 - 0.672 1.263 2.226
ENSG00000177034 E005 49.0451084 0.44398940 0.2388681084 0.475292904 5 79979777 79980263 487 - 1.387 1.941 1.885
ENSG00000177034 E006 220.3584111 1.30609058 0.5867522538 0.761431929 5 79980264 79982434 2171 - 2.073 2.448 1.255
ENSG00000177034 E007 133.6737768 1.09994606 0.7572770985 0.871031691 5 79982435 79983794 1360 - 1.886 2.110 0.750
ENSG00000177034 E008 8.6293144 0.04512240 0.0261364202 0.115747393 5 79985571 79985659 89 - 0.807 0.765 -0.170
ENSG00000177034 E009 43.4690345 0.08622137 0.0005891566 0.006483239 5 79986950 79987107 158 - 1.449 1.343 -0.366
ENSG00000177034 E010 36.6106229 0.24998582 0.0003739715 0.004478139 5 79988239 79988315 77 - 1.399 0.919 -1.720
ENSG00000177034 E011 37.9283529 0.31254097 0.0066625712 0.043009763 5 79988462 79988538 77 - 1.405 1.113 -1.026
ENSG00000177034 E012 35.0881200 0.20760097 0.0100446949 0.058346567 5 79988539 79988644 106 - 1.362 1.224 -0.485
ENSG00000177034 E013 30.3055262 0.21552438 0.0219900002 0.102545729 5 79989152 79989244 93 - 1.298 1.190 -0.382
ENSG00000177034 E014 33.6515488 0.24848981 0.0401895689 0.155171411 5 79990160 79990236 77 - 1.338 1.264 -0.260
ENSG00000177034 E015 25.6856063 0.27559637 0.0209233635 0.099090002 5 79990594 79990663 70 - 1.236 1.060 -0.627
ENSG00000177034 E016 13.0326380 0.35147180 0.0135277596 0.072466239 5 79991158 79991262 105 - 0.976 0.597 -1.517