ENSG00000176473

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335290 ENSG00000176473 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR25 protein_coding protein_coding 13.55581 19.59087 6.417178 2.989452 0.4871161 -1.608661 0.4916354 1.182577 0.0000000 0.3543285 0.0000000 -6.897938 0.03186667 0.06363333 0.00000000 -0.06363333 0.0005906258 0.0005906258 FALSE TRUE
ENST00000402312 ENSG00000176473 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR25 protein_coding protein_coding 13.55581 19.59087 6.417178 2.989452 0.4871161 -1.608661 2.1145483 2.947294 1.5000621 0.5125863 0.4561199 -0.969670 0.17489167 0.15343333 0.24486667 0.09143333 0.7982119154 0.0005906258 FALSE TRUE
ENST00000554998 ENSG00000176473 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR25 protein_coding protein_coding 13.55581 19.59087 6.417178 2.989452 0.4871161 -1.608661 2.2436459 3.118772 1.2470909 0.5731568 0.3711501 -1.315507 0.16245000 0.16120000 0.18850000 0.02730000 0.9365052606 0.0005906258 FALSE TRUE
ENST00000555775 ENSG00000176473 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR25 protein_coding processed_transcript 13.55581 19.59087 6.417178 2.989452 0.4871161 -1.608661 6.5126764 10.055308 2.9361814 1.8005627 0.3173138 -1.772473 0.46307500 0.50813333 0.45793333 -0.05020000 0.8118546811 0.0005906258 FALSE TRUE
MSTRG.10189.11 ENSG00000176473 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR25 protein_coding   13.55581 19.59087 6.417178 2.989452 0.4871161 -1.608661 1.3958298 1.332445 0.3747315 0.2229840 0.1873797 -1.802939 0.10677917 0.06806667 0.05716667 -0.01090000 0.8895569756 0.0005906258 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000176473 E001 2.0000843 0.0108647759 0.22312138 0.45710907 14 100376418 100376432 15 + 0.215 0.470 1.608
ENSG00000176473 E002 23.8477305 0.1650154760 0.39730744 0.62618390 14 100376433 100376484 52 + 1.225 1.321 0.337
ENSG00000176473 E003 27.2297677 0.1788661921 0.37573983 0.60880313 14 100376485 100376493 9 + 1.269 1.377 0.376
ENSG00000176473 E004 27.7651944 0.1985019327 0.43487202 0.65490725 14 100376494 100376495 2 + 1.297 1.381 0.294
ENSG00000176473 E005 7.3119553 0.0043522021 0.93012430 0.96706623 14 100376496 100376499 4 + 0.829 0.846 0.065
ENSG00000176473 E006 8.5335555 0.0115846388 0.72123812 0.84890930 14 100376500 100376504 5 + 0.938 0.893 -0.170
ENSG00000176473 E007 1.8084964 0.2249707374 0.40279165 0.63059251 14 100376505 100376540 36 + 0.215 0.436 1.433
ENSG00000176473 E008 5.9890838 0.0066842502 0.01362095 0.07286660 14 100376541 100376704 164 + 0.358 0.816 2.116
ENSG00000176473 E009 0.8682213 0.0463357216 0.22875246   14 100376755 100376809 55 + 0.000 0.294 9.804
ENSG00000176473 E010 0.3088520 0.0273081633 0.31306513   14 100377514 100377545 32 + 0.215 0.065 -1.978
ENSG00000176473 E011 122.7745756 0.0026177021 0.01228305 0.06757408 14 100380910 100381746 837 + 2.071 1.974 -0.328
ENSG00000176473 E012 0.0000000       14 100382000 100382151 152 +      
ENSG00000176473 E013 3.6540099 0.0292071123 0.87268630 0.93741199 14 100397185 100397259 75 + 0.622 0.594 -0.123
ENSG00000176473 E014 0.5233527 0.0218246747 0.08213055   14 100454459 100455512 1054 + 0.358 0.066 -2.971
ENSG00000176473 E015 85.3603652 0.0083988042 0.75229233 0.86809410 14 100468021 100468168 148 + 1.825 1.849 0.081
ENSG00000176473 E016 0.2027342 0.0358742924 1.00000000   14 100476474 100476581 108 + 0.000 0.066 7.381
ENSG00000176473 E017 95.9251021 0.0013128859 0.23250911 0.46806936 14 100483994 100484124 131 + 1.931 1.886 -0.152
ENSG00000176473 E018 0.0000000       14 100503700 100503787 88 +      
ENSG00000176473 E019 0.0000000       14 100504497 100504697 201 +      
ENSG00000176473 E020 76.4523348 0.0006388995 0.83888202 0.91877324 14 100525870 100525945 76 + 1.781 1.797 0.053
ENSG00000176473 E021 95.5597612 0.0007027139 0.61369426 0.77937946 14 100525946 100526040 95 + 1.865 1.892 0.092
ENSG00000176473 E022 116.5630306 0.0006352871 0.40177679 0.62985902 14 100529068 100529208 141 + 1.941 1.980 0.129
ENSG00000176473 E023 2.9835832 0.0083056508 0.31113269 0.55153203 14 100529209 100529624 416 + 0.358 0.557 1.028
ENSG00000176473 E024 168.8817205 0.0008778867 0.96782375 0.98577462 14 100529820 100530303 484 + 2.129 2.134 0.015